Labshake search
Citations for New England Biolabs :
5951 - 6000 of 9547 citations for QuantiChrom Phosphate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: pals-17::gfp and pals-20::wrmScarlet translational reporter constructs were assembled using NEBuilder HiFi kit (New England Biolabs) by fusing PCR products and gene fragments ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: DNA for WGS was prepared by PCR-free library preparation according to the NEBNext DNA Ultra II kit (NEB). Sequencing was performed by Illumina NextSeq 2000 P3 150PE.
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bioRxiv - Immunology 2023Quote: 1 μg of total RNA was subjected to rRNA depletion using the NEBNext rRNA Depletion Kit (New England Biolabs), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... of ATG13 and ATG13.11-198 referred to as ATG13.1HORMA were introduced into linearised pBMN-HA-C1 using Gibson Cloning kit (New England Biolabs) to create pBMN-HA-ATG13 and ATG13.1HORMA (Addgene #186223 and #18622 respectively) ...
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bioRxiv - Molecular Biology 2023Quote: DNA templates were transcribed and translated in vitro using a custom PURExpress ΔRF-123 ΔRibosome Kit (New England Biolabs) and a final concentration of 2.2 μM ribosomes ...
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bioRxiv - Biochemistry 2023Quote: ... Sequencing libraries were prepared using NEBNext Ultra II RNA library prep kit using the manufacturer’s instructions (NEB, Ipswich, MA). Sequencing libraries were validated on the Agilent TapeStation and quantified using Qubit 2.0 Fluorometer as well as via quantitative PCR (KAPA Biosystems ...
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bioRxiv - Cancer Biology 2023Quote: ... mRNA expression was determined using SYBR green Luna Universal One-step RT-PCR kit (New England Biolabs, Cat #E3005L) with a BioRad CFX96 thermocycler ...
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bioRxiv - Plant Biology 2023Quote: ... Poly-A enriched libraries were prepared using the NEBNext UltraTM RNA Library Prep Kit (New England Biolabs, Ipswich, USA) for Illumina ...
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bioRxiv - Physiology 2023Quote: ... cDNA was generated from 500ng of RNA using the LunaScript RT Supermix kit (New England Biolabs, Cat. No. E3010L) according to the manufacturer’s directions ...
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bioRxiv - Systems Biology 2023Quote: ... metatranscriptomic libraries were constructed according to the NEBNext® UltraTM RNA Library Prep Kit (Illumina, New England Biolabs, MA) and sequenced on the Illumina Hiseq 2500 platform (Illumina ...
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bioRxiv - Systems Biology 2023Quote: ... Dual-indexed sequencing libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) with 10 ng or 10 uL of input DNA (whichever was lower volume) ...
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bioRxiv - Immunology 2023Quote: ... A 140-500 ng aliquot of total RNA was rRNA depleted using NEB’s Human/Mouse/Rat RNAse-H based Depletion kit (New England BioLabs). Following rRNA removal ...
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bioRxiv - Genomics 2023Quote: ... Preparing the MLI1 DNA library was performed using NEBNext Ultra II DNA Library Prep kit (New England Biolabs, USA) and sequencing on an Illumina MiSeq with paired-end 300 bp reads at the “Molecular and Cellular Biology” core facility of the IMCB SB RAS (Novosibirsk ...
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bioRxiv - Cell Biology 2023Quote: ... APOE3-C-mEm ΔSS were created from APOE3-mEm using Q5 Site-Directed Mutagenesis Kit (New England Biolabs, E0554S). The N-terminal domain constructs consist of residues 19-209 with or without residues 1-18 of the N-terminal signal peptide ...
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bioRxiv - Developmental Biology 2023Quote: ... Eluted DNA fragments were amplified using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ChIP sequencing libraries were prepared using NEBNext® Ultra™ II DNA Library Prep Kit reagents (NEB, Ipswitch, MA) according to the procedure recommended by the manufacturer ...
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bioRxiv - Microbiology 2023Quote: ... which was subsequently cloned into linearized pUHE-21 (106) using NEBuilder HiFi DNA Assembly Cloning Kit (New England BioLabs). Construct was verified by Sanger DNA sequencing with primers 146 and 156.
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bioRxiv - Microbiology 2023Quote: The pGEX-6P-PrkA1-338 plasmid (Table 2) was constructed by a two-part ligation (NEB Quick Ligation kit) of BamHI- and NotI-linearized pGEX-6P and PCR-generated prkA1-338 amplified with primers AS21 and AS81 (Table 3 ...
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bioRxiv - Molecular Biology 2023Quote: ... 15 µL 100 µM dNTPs, 0.3 µL 50 mg/mL BSA, 6 µL blunting enzyme mix from quick blunting kit, NEB). Prior to ligation ...
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bioRxiv - Immunology 2023Quote: ... RNA sequencing libraries were produced by using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770L). Quantification of the library was performed using a dsDNA HS Assay Kit and Qubit (Molecular Probes ...
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bioRxiv - Molecular Biology 2023Quote: ... The sequencing library was generated using a NEBNext Ultra DNA Library Prep Kit for Illumina (New England BioLabs, USA). The concentration and quality of the library was analysed with a Qubit 2.0 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... low range ssRNA ladder (N0364S) and the Monarch RNA Cleanup Kit (T2030L) were purchased from NEB (Ipswich, MA, USA). T4 DNA ligase (EL0013) ...
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bioRxiv - Plant Biology 2023Quote: ... the cDNA libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). The resulting libraries were measured using the Qubit dsDNA High Sensitivity Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... mRNA was reverse transcribed into cDNA using ProtoScript® II First Strand cDNA Synthesis Kit (BioLabs, Ipswich, MA, USA). Ribosomal Protein L32 (RPL32 ...
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bioRxiv - Cell Biology 2023Quote: ... Envelope gene mutations were introduced into WT cDNA sequence using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). All cloned were sequenced (Eurofins).
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bioRxiv - Genomics 2023Quote: ... libraries were constructed with the NEBNext Small RNA Library Preparation Kit according to manufacturer’s recommendations (NEB, cat. no. E7330). See Supplemental File 6 for detailed protocol.
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bioRxiv - Cancer Biology 2023Quote: Kinase-dead mutant (K37R) of Nek2A was derived from Nek2A in pJP1563 using Q5 Site-Directed Mutagenesis Kit (NEB) following the instructions of manufacturer ...
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bioRxiv - Developmental Biology 2023Quote: RNA was isolated from 17 hpf Ciona robusta larvae by Monarch Total RNA miniprep kit (New England BioLabs Inc.). cDNA was produced by Omniscript reverse transcription kit (QIAGEN) ...
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bioRxiv - Cell Biology 2023Quote: ... Library preparation followed the NEBNext Ultra II DNA Library Prep Kit for Illumina protocol (New England Biolabs, MA, US). Size selection of adapter-ligated DNA was performed as described in the protocol ...
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bioRxiv - Microbiology 2023Quote: ... The complementation plasmid was constructed by Gibson assembly using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs). In brief ...
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bioRxiv - Plant Biology 2023Quote: ... the SQK-RNA002 kit (Oxford Nanopore Technologies) was used together with NEBNext® Quick Ligation Reaction Buffer (NEB B6058), T4 DNA Ligase 2M U/ml (NEB M0202) ...
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bioRxiv - Microbiology 2023Quote: ... and DNA libraries were prepared using the NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs, NEB). DNA purifications and size selections were made using Ampure XP beads (Beckman Coulter ...
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bioRxiv - Microbiology 2023Quote: ... and DNA libraries were prepared using the NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs, NEB). DNA purifications and size selections were made using Ampure XP beads (Beckman Coulter ...
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bioRxiv - Cell Biology 2023Quote: ... 200ng of cleaned DNA was used as an input for conversion using a Enzymatic Methyl-seq Kit (NEB E7120S) according to the manufacturer’s recommended protocol ...
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bioRxiv - Genetics 2023Quote: Whole genome DNA methylation sequencing was performed using the NEBNext Enzymatic Methyl-seq (EM-seq) Kit (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Non-β-lactamase genes used as controls were cloned into the pBAD18 using the Gibson Assembly Cloning Kit (NEB). pBAD (RBS)_fwd/rev primer pairs were used to amplify the plasmid backbone ...
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bioRxiv - Microbiology 2023Quote: cDNA was generated starting from 150 ng of RNA using ProtoScript® II First Strand cDNA Synthesis Kit (NEB) using random hexamers in agreement with the manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2023Quote: ... and RNA libraries were prepared using NEBNext® Ultra TM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s protocols ...
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bioRxiv - Synthetic Biology 2023Quote: ... In vitro transcription of sgRNAs was performed using the HiScribe™ T7 Quick High Yield RNA Synthesis Kit (NEB). Then ...
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bioRxiv - Microbiology 2023Quote: ... and RNA-Seq libraries were generated using the NEBNext® Ultra II Directional RNA Library Prep Kit (NEB # E7760L). Two biological replicates were sequenced using the Illumina NextSeq 550 platform to generate 150 bp PE reads.
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bioRxiv - Molecular Biology 2023Quote: ... and cDNA libraries were prepared using NEBNext® UltraII RNA library preparation kit (NEB, New England Biolabs, MA, USA) using the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... and cDNA libraries were prepared using NEBNext® UltraII RNA library preparation kit (NEB, New England Biolabs, MA, USA) using the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequencing libraries were prepared using 50ng RNA according to the manufacturer’s recommendation for the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) and sequenced on the Illumina NextSeq 500.
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bioRxiv - Molecular Biology 2023Quote: ... The PCR product was cleaned and concentrated with the Zymo Research kit following the manufacturer’s protocol and then digested by DpnI (New England BioLabs) overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... The pNH605-RIM11 was amplified by making use of the Q5® Site-Directed Mutagenesis Kit (New England Biolabs) following the instructions of the manufacturer to generate pNH605-RIM11 Y199F ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA fragments were then constructed into libraries using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645). 100M reads/read pairs per library were requested ...
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bioRxiv - Plant Biology 2023Quote: ... ChIP DNA libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... This was followed by two sets of enzymatic conversion steps using the Enzymatic Methyl-seq kit (NEB, Cat# E7120L) to differentiate cytosines from 5mC and 5hmC ...
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bioRxiv - Neuroscience 2023Quote: ... followed by preparation of RNA libraries using the NEBNext Ultra RNA Prep kit (NEB #E7530) (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Neuroscience 2023Quote: ... followed by preparation of RNA libraries using the NEBNext Ultra RNA Prep kit (NEB #E7530) (New England Biolabs (NEB), Ipswich ...