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Citations for New England Biolabs :
551 - 600 of 10000+ citations for rno mir 542 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: ... The PCR products from all reactions were pooled and purified with the Monarch PCR & DNA Cleanup Kit (NEB) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2020Quote: ... Sequenced PCR products showing overlapping peaks in their chromatograms were cloned using NEB PCR cloning kit (NEB #E1202S) and then sequenced to define the identity of mutations carried by F1 heterozygous animals ...
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bioRxiv - Microbiology 2019Quote: ... the PCR products were purified using Monarch® PCR & DNA Cleanup Kit (5 μg; New England Biolabs GmbH). For the second PCR ...
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bioRxiv - Biophysics 2022Quote: ... PCR cleanup was carried out on each amplicon sample using the Monarch PCR cleanup kit (New England Biolabs). The amplicons were then dual-indexed for paired-end read Illumina sequencing using Nextera primers N701 and N702 for the PEL and SUP of the 5.5 h time point and N705 and N706 for the PEL and SUP of the 9.0 h time point ...
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bioRxiv - Microbiology 2021Quote: ... Reverse transcription and qRT-PCR analysis was performed by Luna universal one- step qRT-PCR kit (NEB # E3005L) with primers list in Table 9.
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bioRxiv - Microbiology 2024Quote: ... The PCR product of the selected deletion mutant was purified using Monarch PCR and DNA cleanup kit (NEB) and sent for sequencing at Eurofins Genomics LLC (Louisville ...
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bioRxiv - Molecular Biology 2023Quote: The amplicons from the Q5 PCR step were cleaned up using Monarch® PCR & DNA Cleanup Kit (NEB). The purity and size of the DNA bands were visualized by running a 1% agarose gel ...
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bioRxiv - Synthetic Biology 2024Quote: ... and PCR products were purified with Monarch PCR and DNA Cleanup kit (New England Biolabs, Ipswich, Massachusetts, USA).
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bioRxiv - Zoology 2024Quote: ... PCR products were purified with a Monarch PCR & DNA clean-up kit (New England Biolabs, Whitby, ON, Canada) and used as template to reamplify the products to incorporate a Kozak translation initiation sequence prior to the start codon (Kozak ...
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bioRxiv - Molecular Biology 2023Quote: ... three 25 μl PCR reactions were pooled and purified using Monarch PCR & DNA Cleanup Kit (5 μg; NEB), according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... and amplified PCR fragments were purified by column purification using the Monarch PCR Cleanup Kit (New England Biolabs) following the manufacturer’s protocols.
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bioRxiv - Biochemistry 2024Quote: ... PCR products were analyzed by agarose gel electrophoresis and purified with Monarch PCR and DNA Cleanup Kit (NEB). On a passivated glass coverslip ...
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bioRxiv - Microbiology 2024Quote: ... the PCR amplicons were purified used the Monarch PCR and DNA Cleanup Kit (New England Biolabs, Whitby, Canada) and sent for Sanger sequencing at the McMaster Genomics facility ...
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bioRxiv - Physiology 2021Quote: ... The full length ayRhp1 sequence was obtained following two rounds of RT-PCR using Phusion High Fidelity taq polymerase (New England Biolabs, Ipswitch, MA, USA) and NucleoSpin gel purification (Macherey-Nagel ...
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bioRxiv - Molecular Biology 2022Quote: ... RT-qPCR was performed by using Luna Universal One-Step RT-qPCR reagents (New England Biolabs). Primers targeting the MS2 stem-loop regions were used for quantifying repeat RNA expression levels ...
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bioRxiv - Neuroscience 2023Quote: ... RT-qPCR was performed by using Luna Universal One-Step RT-qPCR reagents (New England Biolabs), following the manufacturer’s instructions.
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bioRxiv - Evolutionary Biology 2021Quote: ... Combinations of indexed Ion Torrent primers were used to amplify barcodes in the library for each time-point (Figure S5 D) using Phusion High-Fidelity PCR (New England BioLabs). To avoid sampling bias during PCR amplification ...
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bioRxiv - Biochemistry 2023Quote: ... DNA synthesis was visualized by detecting biotin with the Phototope-Star detection kit (New England Biolabs Ref N7020S) and images acquired on a Chemidoc system (Biorad).
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bioRxiv - Genomics 2021Quote: ... DNA was purified using Monarch® PCR & DNA Cleanup Kit (NEB), using the following recommended protocol modification ...
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bioRxiv - Cell Biology 2021Quote: ... DNA was purified using the Monarch PCR & DNA Cleanup Kit (NEB), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and purified using Monarch PCR clean-up kit (New England Biolabs). Blunted DNA was A-tailed using NEBNext dA-tailing kit and column-purified ...
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bioRxiv - Biochemistry 2020Quote: ... purified with Monarch PCR & DNA Cleanup Kit (New England BioLabs, USA) and cloned into PET-24a (+ ...
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bioRxiv - Developmental Biology 2021Quote: ... The PCR product was transcribed using the T7 IVT Kit (NEB), followed by DNase I (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... and purified using the Monarch PCR & DNA Cleanup Kit (NEB, T1030L). The source of the 3xHA-Nluc starting CDS (“Nluc start” ...
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bioRxiv - Synthetic Biology 2021Quote: ... followed by purification with Monarch® PCR & DNA Cleanup Kit (NEB). The Mobius Assemblies were verified first by colony PCR using GoTaq® Green Master Mix (Promega ...
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bioRxiv - Plant Biology 2021Quote: ... Amplicons were purified with Exo-CIP Rapid PCR Cleanup Kit (NEB) following supplier’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... or the Exo-Cip Rapid PCR Cleanup Kit (New England Biolabs). Gene editing efficiency was analyzed using TIDE analysis software (Brinkman et al ...
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bioRxiv - Microbiology 2021Quote: ... cleaned up using the Monarch PCR Purification kit (New England Biolabs), and used in a Gibson assembly reaction (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: PCR amplicons and plasmids were purified using Monarch DNA kits (NEB). PCR ...
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bioRxiv - Genomics 2021Quote: ... and DNA cleaned with Monarch PCR & DNA Cleanup Kit (NEB T1030S). Resulting supercoiled plasmid is linearized at the 3’ end of the PolyA tail using BamHI-HF (NEB R3136S) ...
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bioRxiv - Molecular Biology 2022Quote: ... Reactions were quenched with Monarch PCR & DNA Cleanup Kit (NEB T1030S) and the purified DNA eluted in 20 µL EB buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Assembled constructs were purified using Monarch PCR purification kit (NEB, T1030S). The assembled plasmid library was electroporated into electrocompetent bacteria (NEB ...
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bioRxiv - Cell Biology 2022Quote: ... Assembled constructs were purified using Monarch PCR purification kit (NEB, T1030S) and used to electroporate into electrocompetent bacteria (NEB ...
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bioRxiv - Microbiology 2022Quote: ... PCR products and plasmids were purified with Monarch DNA kits (NEB). Overlapping primers for amplification were designed using the NEBuilder assembly tool (https://nebuilderv1.neb.com/ ...
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bioRxiv - Molecular Biology 2024Quote: ... and purified using a post-PCR cleanup kit (New England Biolabs) per manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... and Luna Universal one-step qRT-PCR kit (New England BioLabs) as previously described (13 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we used the kit Phusion High-Fidelity PCR (New England, Biolabs), containing Phusion Buffer 1X ...
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bioRxiv - Genetics 2023Quote: ... amplicons were purified using Monarch® PCR & DNA Cleanup Kit (NEB).
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted with the Monarch PCR & DNA Cleanup kit (NEB). Sequencing libraries were prepared with the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... PCRs were undertaken using the Q5 High-Fidelity Polymerase kit (NEB) and thermocycling conditions of 98°C ...
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bioRxiv - Neuroscience 2023Quote: ... and Sanger sequenced using the NEB PCR Cloning Kit (NEB, #E1202S).
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bioRxiv - Systems Biology 2024Quote: ... followed by purification using a Monarch PCR&DNA Cleanup Kit (NEB). Ring-ligation was carried out using T4 DNA ligase (NEB ...
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bioRxiv - Synthetic Biology 2024Quote: ... and purified with the Monarch® PCR & DNA Cleanup Kit (NEB). An aliquot (1 µg ...
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bioRxiv - Physiology 2020Quote: Input RNA (50-100 ng/µL) was reverse-transcribed using LunaScript® RT SuperMix Kit (New England BioLabs) or TaqMan MicroRNA Reverse Transcription Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... cDNAs were synthesized from RNA using the LunaScript™ RT SuperMix Kit from New England BioLabs (NEB, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... The qPCR was performed using the NEB Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) with cycling conditions of 10 min at 55°C for reverse transcription ...
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bioRxiv - Developmental Biology 2021Quote: ... 10 ng RNA were used for analysis with the LUNA one-step RT-qPCR kit (LUNA E3005L Biolabs). The relative expression levels of the mRNA of interest were determined by real-time PCR using Quantifast SYBR Green Mix (Qiagen ...
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bioRxiv - Neuroscience 2022Quote: ... Complementary DNA (cDNA) was synthesized from 200ng RNA using LunaScript® RT-SuperMix kit (New England Biolabs #E3010). RT-qPCR reactions were performed using Luna® Universal qPCR Master Mix (New England Biolabs #M3003) ...
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bioRxiv - Microbiology 2022Quote: ... viral cDNA was synthesized from the extracted RNA by using a LunarScript RT SuperMix Kit (New England BioLabs). The DNA was then amplified by performing a multiplexed PCR in two pools using the ARTIC-N5 primers and the Q5 Hot Start DNA polymerase (New England BioLabs ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA was then directly used in the Luna® Universal One-Step RT-qPCR Kit (New England BioLabs). Primers listed in supplemental table 10.