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Citations for New England Biolabs :
551 - 600 of 9263 citations for QuantiChrom Hyaluronidase Inhibitor Screening Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: EnGen sgRNA synthesis kit (NEB)
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bioRxiv - Genomics 2022Quote: Monarch RNA Cleanup kit (NEB)
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bioRxiv - Microbiology 2022Quote: ... LunaScriptTMSuperMix Kit (New England BioLabs), SYBR qPCR Master Mix (ChamQTMUniversal ...
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bioRxiv - Microbiology 2022Quote: ... or NEBase Changer–Kit (NEB) and primers listed in supplementary Table 1 ...
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bioRxiv - Genetics 2022Quote: ... Gibson assembly cloning kit (NEB) was applied to ligate vector and GFP∷PAB-1∷3xFLAG fragment to generate plasmid pQZ2 ...
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bioRxiv - Microbiology 2021Quote: ... The A-Tailing Kit (NEB) was then used to add additional deoxyadenosine (A ...
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bioRxiv - Neuroscience 2022Quote: ... Quick Ligation Kit (M2200L, NEB) was used for the ligation of these appropriate clone ...
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bioRxiv - Biochemistry 2022Quote: ... using Q5 mutagenesis kit (NEB). N-(-42D ...
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bioRxiv - Cell Biology 2022Quote: ... using Gibson Assembly kit (NEB). To test the interaction of KRP4 and KRP5 ...
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bioRxiv - Microbiology 2022Quote: ... LunaScript RT SuperMix Kit (NEB) was used to generate cDNA using 1μg RNA ...
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bioRxiv - Plant Biology 2023Quote: ... blunting (Quick Blunting kit, NEB), and re-ligating using T4 DNA Ligase (NEB).
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bioRxiv - Biophysics 2023Quote: ... Gibson Assembly Kit (#E5510S, NEB) was used following the manufacturer’s instruction ...
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bioRxiv - Microbiology 2023Quote: ... Q5 DNA polymerase kit (NEB) was used to make amplicons ...
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bioRxiv - Genomics 2023Quote: ... or RNA cleanup kit (NEB) according to manufacturer instructions ...
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bioRxiv - Synthetic Biology 2023Quote: Monarch Plasmid MiniPrep kit (NEB) for isolating template plasmid and assembled plasmid from E ...
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bioRxiv - Molecular Biology 2020Quote: ... 40 μg/ml phenylmethylsulfonyl fluoride and protease inhibitor) and resuspended again in 1 ml MNase digestion buffer with 1,250 Units MNase (NEB Biolabs). Chromatin-MNase mix was incubated at 37 °C for 30 min ...
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bioRxiv - Molecular Biology 2020Quote: ... The beads were resuspended in Elution Buffer (Wash Buffer supplemented with TEV protease and Murine RNase Inhibitor (New England Biolabs)) and the bound Enp1-TAP containing complexes were eluted for 1.5 – 2 hours ...
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bioRxiv - Biochemistry 2020Quote: ... was incubated for 30 minutes shaking at 1.500 rpm at room temperature with protein extract (500 µg of whole cell protein extract or 150 µg of CPE) and murine RNase Inhibitor (NEB) in the corresponding protein extraction buffer (without glycerol) ...
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bioRxiv - Microbiology 2019Quote: ... followed by size exclusion chromatography (HiLoad 16/60 Superdex 200) using the following buffers: lysis buffer (wash buffer supplemented with protease inhibitor, DNase I [NEB]), wash buffer (20mM HEPES-KOH pH 8.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... The 5’ end phosphorylation was performed by treating 10 μg total RNA with 0.5 μl of RNase inhibitor (20U final, NEB M0307L), 1 μl of 10x T4 PNK reaction buffer ...
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bioRxiv - Microbiology 2020Quote: ... Cell pellets were resuspended in 1 ml of iCLIP lysis buffer B (same as buffer A but with 1% v/v NP-40 and 11 μl of Murine RNase inhibitor (NEB) per 1 ml ...
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bioRxiv - Microbiology 2021Quote: ... by removing media and washing cells with cold PBS and subsequently scraped into 50 – 200 μl (depending on dish size) lysis buffer containing protease inhibitor (#5872, NEB). The sample was then left on ice for at least 20 mins and subsequently spun down at 20,000 x g for 10 mins at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... 10 mM sodium ascorbate before collecting them by scraping in 1 mL of the same solution with Murine RNase Inhibitor (1:1000, NEB). Cells were collected in a microcentrifuge tube ...
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bioRxiv - Biochemistry 2021Quote: ... 5 μl RT mastermix (2x reaction buffer, 20 mM DTT, 4U murine RNase inhibitor [NEB], 30 U ProtoScript II Reverse Transcriptase [NEB]) were added ...
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bioRxiv - Biochemistry 2021Quote: ... per 1g of wet cell weight and 1 Unit of 30 Units/μL RNase Inhibitor (from human placenta; New England Biolabs (NEB), No ...
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bioRxiv - Molecular Biology 2021Quote: The 5’ end phosphorylation was performed by treating 10 μg of total RNA extract with 0.5 μl of RNase inhibitor (20U final, NEB M0307L), 1 μl of 10x T4 PNK reaction buffer ...
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bioRxiv - Immunology 2020Quote: ... Single antigen specific memory B cells were sorted on BD FACS Aria II into 96-well PCR plates (Axygen) containing 10 μl per well of lysis buffer (10 mM DPBS, 4 U Mouse RNase Inhibitor, NEB). Plates were immediately frozen on dry ice and stored at 80 C or processed for cDNA synthesis.
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bioRxiv - Genomics 2021Quote: ... and the cells was washed once with wash buffer (PBS with 1% BSA and 40 units/ml of RNase inhibitor, murine (NEB)) ...
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bioRxiv - Molecular Biology 2022Quote: ... The lysate obtained from lysis with 1% NP-40 lysis buffer phosphatase inhibitor were mixed with lambda phosphatase (New England Biolabs) and incubated at 30°C for 2 hours ...
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bioRxiv - Microbiology 2023Quote: ... 200 μl reactions were incubated with 100 μl Ni-NTA beads equilibrated in 800 μl Ni-NTA buffer A with 10 mM imidazole and 40 U murine RNase inhibitor (NEB) at 4 °C for 1 h ...
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bioRxiv - Genetics 2023Quote: ... 0.1% v/v Tween-20 in DPBS) supplemented with 0.1% v/v murine RNase inhibitor (MRI) (New England Biolabs, M0314L) for 30 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... or A3H Hap VII were added to the reactions together with 40 U of murine RNase inhibitor (NEB, Ipswich, Massachusetts). Activity of cell lysates was evaluated in reactions containing 43 nt or 85 nt linear or 21 nt hairpin substrates (Table S3) ...
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bioRxiv - Genomics 2023Quote: ... of RNA from each sample were pooled into a single sample and treated with DNaseI with added RNase Inhibitor (New England Biolabs), then purified using MonarchR RNA Cleanup Kit (New England Biolabs) ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were treated with DNase I (20 units) in the presence of RNase inhibitor at 300 U/ml in x1 buffer # 2 (NEB) at 37°C for 30 min ...
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bioRxiv - Biochemistry 2019Quote: ... Protein concentration was calculated to be 6.2 mg/L using the Bradford assay with bovine serum albumin (New England Biolabs) as a standard.
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bioRxiv - Biochemistry 2019Quote: ... Methylation assays were performed using 100 ng of an unmethylated pUC19 plasmid purified from dam- /dcm- E.coli strain (C2925I, NEB) or a 1-kb fragment containing 14 CpG sites amplified from the Meis1 enhancer were used as DNA substrates for filter binding assays ...
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bioRxiv - Biochemistry 2020Quote: ... The toeprinting assay was performed as previously described (Orelle et al., 2013) using the PURExpress system (New England Biolabs). When needed ...
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bioRxiv - Biochemistry 2022Quote: Native 3’ end extension assays experiments where set-up as describe in the D-loop assays with the exception that dNTP’s (1 mM) and Klenow (exo-)(NEB) were added after 10 minutes of D-loop formation ...
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bioRxiv - Neuroscience 2020Quote: ... soluble supernatants were immunopreciptated with monoclonal anti-GFP antibody precoupled to Protein G Sepharose beads and immunoprecipitates were washed extensively with IPB containing 0.5M NaCl followed by further washes with IPB and then with lambda phosphatase assay buffer (NEB). Equal volumes of each immunoprecipitated sample were then aliquoted into two tubes and beads were then resuspended with 100 microliters of lambda phosphatase assay buffer containing 400 units of lambda protein phosphatase (NEB) ...
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bioRxiv - Biochemistry 2020Quote: The total volume of CRISPR Cas12a-based cleavage assay was 20 μL including LbCas12a protein (New England BioLabs, Inc.), crRNA ...
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bioRxiv - Systems Biology 2020Quote: ... The cleavage activities of the gRNAs were validated using the T7E1 assay according to the manufacturer’s instructions (NEB, #E3321). The sgRNA VIM-AS3 (5’-CTAAATTATCCTATATATCA-3’ ...
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bioRxiv - Biochemistry 2021Quote: Recombinant SARS-CoV-2 S protein constructs HexaFurin and HexaPro were concentrated to 0.5 mg/mL in Assay Buffer and incubated with the indicated concentrations of furin protease (NEB) or dasTMPRSS2 ...
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bioRxiv - Microbiology 2021Quote: RNase III assay: 1 μg double-stranded (ds) RNA substrates were digested with either ShortCut® RNase III (NEB) or RNase III (6xHis ...
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bioRxiv - Microbiology 2022Quote: ... a product flanking the sgRNA target sites was PCR-amplified and subjected to a T7 Endonuclease I assay (NEB) to detect indels ...
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bioRxiv - Microbiology 2022Quote: ... Conventional PCR assays were carried out with Q5 Hot Start High-Fidelity 2× Master Mix (New England Biolabs, Australia). For some samples ...
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bioRxiv - Genetics 2023Quote: ... and F2 crispants were fin clipped at 5 weeks and genotyped by a T7EI assay (M0302, New England Biolabs). We extracted DNA using the Quick-DNA Microprep Kit (D3021 ...
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bioRxiv - Developmental Biology 2023Quote: ... The sgRNA target region was amplified from transfected cells and analyzed by T7 endo1 nuclease assay (New England Biolabs). sgRNA ...
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Mechanism of RanGTP priming H2A-H2B release from Kap114 in an atypical RanGTP•Kap114•H2A-H2B complexbioRxiv - Biochemistry 2023Quote: Pull-down binding assays were performed by immobilizing purified His6MBP or His6MBP-Kap114 on amylose resin (New England BioLabs). Resin was stored in Binding Assay (BA ...
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bioRxiv - Molecular Biology 2023Quote: LAMP assays were developed with the Warm Start Colorimetric LAMP 2X Master Mix (DNA & RNA) (New England Biolabs, USA), using a mixture of LAMP oligonucleotides at a final concentration of 1.6 μM FIP and BIP ...
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bioRxiv - Cell Biology 2019Quote: ... stained with oligo-conjugated WGA at a concentration of 2-5 μg/mL in 1× HBSS with 2000× diluted murine RNase inhibitor (New England Biolabs, M0314L) at 37 °C for 20 min ...