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Citations for New England Biolabs :
551 - 600 of 1813 citations for Primary Human Coronary Artery Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... coli 5-alpha cells (New England BioLabs). Following mutagenesis for fluorescent labeling and/or creating RTT mutations using the Q5 mutagenesis kit (New England BioLabs) ...
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bioRxiv - Bioengineering 2023Quote: ... coli BL21 (DE3) cells (New England Biolabs) harbouring a pETDuet-1 expression plasmid containing the codon-optimised TmEnc gene (UniProt ...
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bioRxiv - Cell Biology 2023Quote: ... The Oxiselect Comet Assay Kit (Cell-Biolabs) was used as a reference for the experiment ...
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bioRxiv - Plant Biology 2023Quote: ... coli NEB Express cells (New England BioLabs). Bacterial cultures were grown at 37 °C to an OD600 of ∼0.6 ...
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bioRxiv - Biochemistry 2023Quote: ... BL21(DE3) Escherichia coli competent cells (NEB) were transformed with Lpg2248 (LotA ...
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bioRxiv - Immunology 2023Quote: ... Novagen) into Shuffle T7 Express cells (NEB). The cells were grown in 2xYT media supplemented with 100 µg/ml ampicillin and 15 µg/ml of chloramphenicol to OD600 0.8 – 1.0 and then protein production was induced with 0.4 mM IPTG and proceeded for 16 h at 20 °C ...
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bioRxiv - Zoology 2023Quote: ... coli cells (New England Biolabs, Whitby, ON) and grown on LB plates containing Zeocin™ antibiotic overnight at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... coli BL21(DE3) cells (New England Biolabs), as previously descrived [6,10] ...
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bioRxiv - Molecular Biology 2023Quote: ... coli DH5α electrocompetent cells (New England Biolabs). Plasmids were extracted with Qiagen miniprep kits (Qiagen GmbH ...
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bioRxiv - Biophysics 2023Quote: ... coli cells (NEB® 5-alpha, NEB) as per the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... coli cells (NEB® 5-alpha, NEB) as per the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... transformed into electrocompetent DH5α cells (NEB, C2989K), and plated on 2XYT-carbenicillin-1% glucose ...
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bioRxiv - Biophysics 2023Quote: ... coli DH5alpha cells (New England Biolabs, C2987I). Transformants were grown to saturation in 5-8mL of LB media supplemented with ampicillin (100µg/mL ...
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bioRxiv - Biochemistry 2023Quote: ... coli T7 shuffle cells (New England Biolabs) after induction of protein expression by addition of isopropyl-β-D-thiogalacto-pyranoside (IPTG ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli NEB Turbo cells (New England Biolabs) were transformed with each of the Gibson products by heat shock and selected on lysogeny broth (LB ...
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bioRxiv - Microbiology 2023Quote: ... coli C2566 pRARE2 cells (New England Biolabs). Proteins were expressed overnight at 17°C in TB (with 25 µg/mL Kanamycin and 17 µg/mL Chloramphenicol) ...
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bioRxiv - Biochemistry 2023Quote: Escherichia coli BL21(DE3) cells (NEB, USA) were transformed with pET-21b plasmid encoding for N-terminally His-tagged NanoLuc gene ...
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bioRxiv - Cell Biology 2023Quote: ... coli SHuffle cells (New England Biolabs, C3026J) for disulfide bond formation and higher stability (Lobstein et al. ...
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bioRxiv - Systems Biology 2024Quote: ... was electroporated into 10beta electrocompetent cells (NEB). After recovery ...
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bioRxiv - Biochemistry 2024Quote: ... coli BL21 (DE3) cells (New England BioLabs).
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bioRxiv - Biophysics 2023Quote: Escherichia coli BL21 (DE3) cells (#C2527, NEB) were transformed with the appropriate plasmid and grown at 37°C to an OD600 of 0.8 - 1.0 in MOPS-buffered Terrific broth (TB) ...
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bioRxiv - Biochemistry 2024Quote: ... coli BL-21 cells (New England Biolabs) were used for the transformation of pET-28b(+ ...
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bioRxiv - Biochemistry 2024Quote: ... coli NEB C2566 cells (New England Biolabs). The cells were grown at 37°C in TB medium for HINT1 ...
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bioRxiv - Biophysics 2024Quote: ... coli chemically-competent cells (New England BioLabs). Single colonies of transformed cells from agar plates containing 100 μg/mL ampicillin and grown at 37 °C were used for starter cultures using 50 mL LB media in 250 mL shake flasks containing 100 μg/mL ampicillin ...
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bioRxiv - Genetics 2024Quote: ... and transformed into DH5a cells (NEB C2987l). A single cysteine sequence was added to the linker between Met4 and MBP to facilitate maleimide conjugation of fluorophores for imaging ...
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bioRxiv - Genetics 2024Quote: ... coli 10β cells (New England BioLabs, C3019I). Following heat-shock ...
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bioRxiv - Plant Biology 2020Quote: All recombinant CPK28 clones were transformed into lambda phosphatase-expressing BL21 (DE3) E.coli cells for the production of dephosphorylated proteins or into T7 Express cells (New England Biolabs) for production of hyperphosphorylated protein (53) ...
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bioRxiv - Cancer Biology 2020Quote: ... pre-existing histones were first quenched by incubating cells with 5 µM SNAP-cell Block (New England Biolabs; S9106S) for 30 min at 37 °C ...
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bioRxiv - Cell Biology 2019Quote: ... cells were collected and incubated in fresh medium containing 4 μM SNAP-Cell TMR-Star (New England Biolabs, Inc.) for 15 min at 25°C ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were spun down and washed three times in ice cold PBS and the cells incubated with DpnI (NEB) to remove any residual plasmid contamination ...
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bioRxiv - Synthetic Biology 2023Quote: ... Each library was split in two and transformed separately into 5-alpha cells (NEB, 3.5uL DNA in 35uL cells). Plasmid library isolation ...
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bioRxiv - Genomics 2023Quote: ... DNA was extracted from 4.3×10^6 cells using the Monarch HMW DNA Extraction Kit for Cells and Blood (New England Biolabs) with 1400 rpm lysis speed ...
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bioRxiv - Genomics 2023Quote: ... cells in the latter plate were labeled with HaloTag Oregon Green and SNAP-Cell 647-SiR (New England Biolabs) simultaneously according to the manufacturers’ protocols ...
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bioRxiv - Microbiology 2022Quote: ... SNAP labeling with SNAP-Cell 647-SIR was done as described in the manual for SNAP-Cell 647-SIR (NEB). Afterward ...
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bioRxiv - Microbiology 2022Quote: ... SNAP labeling with SNAP-Cell 647-SIR was done as described in the manual for SNAP-Cell 647-SIR (NEB) before imaging ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were grown on glass coverslips and pre-existing SNAP-tagged histones were first quenched by incubating cells with 10 µM of the non-fluorescent substrate SNAP-cell Block (New England Biolabs) for 30 min followed by a 30 min-wash in fresh medium and a 2 hr-chase ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNAs were isolated from Calu-3 infected cells 48h post infection using the Luna Cell Ready Lysis Module (New England Biolabs). Viral RNA were quantified by qRT-PCR in triplicate as described in [27] ...
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bioRxiv - Cell Biology 2021Quote: ... COS-7 cell lysates from FLAG-ACBD4/5 (mFFAT) expressing cells were treated for 1 h with λPP (New England BioLabs) as described above ...
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bioRxiv - Cell Biology 2019Quote: ... To visualize SNAP-tagged dCENP-A proteins cells were labelled with 3 µM SNAP-Cell TMR Star (New England Biolabs) for 30 min ...
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bioRxiv - Immunology 2020Quote: RNA was extracted from 0.25×106 cells at day 12 of cell culture using the Monarch total RNA miniprep kit (New England Biolabs, T2010S) and quantified using the Qubit RNA HS Assay kit (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... Dissociated cells were resuspended in 1ml of Cell Hashing Staining Buffer (1× PBS with 2% BSA (New England Biolabs, B9000S) and 0.02% Tween (Tween®-20 Solution ...
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bioRxiv - Cell Biology 2022Quote: Cytokinetic furrowing rate measurements for both the P0 cell and the AB cell were taken relative to nuclear envelope breakdown (NEB). For all analyses ...
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bioRxiv - Molecular Biology 2023Quote: ... pre-existing SNAP-tagged histones were first quenched by incubating cells with 10 μM of the non-fluorescent SNAP reagent (SNAP-cell Block, New England Biolabs) for 30 min at 37°C followed by a 30 min wash in fresh medium and a 2-hour chase ...
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bioRxiv - Cell Biology 2022Quote: ... 3 h) the cells were incubated in 3 µM SNAP-Cell TMR-Star (New England Biolabs Inc., Boston, MA, USA) in 10% FBS DMEM for 30 min and embedded using Vectashield (supplemented with DAPI).
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bioRxiv - Neuroscience 2022Quote: ... 100 cells were isolated directly into low protein binding eppendorfs containing 2 ul NEBNext Single Cell Lysis Buffer (NEB, E5530S). Samples were kept on dry ice until transfer to −80 C for overnight storage.
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bioRxiv - Molecular Biology 2023Quote: ... The SNAP-tagged histones neosynthesized during the chase time were then pulse-labelled by incubating cells with 2 μM of the red-fluorescent SNAP reagent SNAP-cell TMR star (New England Biolabs) for 15 min at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... coli cells (α-select, XL1-Blue, or BL21-DE3 cells were used, depending on application; all originally sourced from NEB and obtained from lab made stocks ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were labelled with 100 nM Halo-TMR ligand and 100 nM SNAP-Cell 647-SiR ligand (New England Biolabs) for 20 min ...
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bioRxiv - Cell Biology 2022Quote: ... coli cells (α-select, XL1-Blue, or BL21-DE3 cells were used, depending on application; all originally sourced from NEB) for 30 min on ice ...
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bioRxiv - Cell Biology 2023Quote: Poly(A)+ RNA was isolated from nuclei and whole cells (see C2C12 cell fractionation above) using the Magnetic mRNA Isolation Kit (New England Biolabs). Two rounds of binding ...