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Citations for New England Biolabs :
551 - 600 of 10000+ citations for Mouse Protein Jagged 2 JAG2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... H1 was transfected with pre-assembled Cas9 protein (NEB) + crRNA and tracrRNA (Synthego) ...
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bioRxiv - Immunology 2021Quote: ... 90 nM of EnGen® Sau Cas9 protein (NEB), NEB buffer 3.1 (NEB ...
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bioRxiv - Genetics 2020Quote: ... MEFs were protein transfected with recombinant RNase H (NEB) using Project Reagent Transfection Kit (Thermo ...
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bioRxiv - Synthetic Biology 2021Quote: ... The supernatants were used with lambda protein phosphatase (NEB) treatment according to its protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... using a commercially available Cas9 protein (New England Biolabs). The efficiency of creating deletion after co-injection of the sgRNA pair was determined by PCR on genomic DNA extracted from injected embryos using the following primers:
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bioRxiv - Molecular Biology 2022Quote: ... The eluted protein was applied to Amylose resin (NEB) equilibrated with buffer D (20 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... 30 μg of Protein G magnetic beads (NEB #S1430) was washed with 250 μl of Immunoprecipitation Reaction Buffer (150 mM NaCl ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... according to the manufacturer’s instructions (Lambda protein phosphatase, Biolabs). Western blot analysis was followed with an antibody against GFP (1:1000 ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... according to the manufacturer’s instructions (Lambda protein phosphatase, Biolabs). In parallel ...
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bioRxiv - Bioengineering 2023Quote: ... Tagless protein was expressed in Lemo21(DE3) cells (NEB) in LB (10 g Tryptone ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was first purified on amylose resin (NEB) in a buffer containing 20 mM Tris pH 8 ...
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bioRxiv - Cell Biology 2023Quote: ... Biotinylated proteins were separated on magnetic streptavidin resin (NEB) and eluted with 2× SDS loading buffer supplemented with 4% SDS and 10 mM Β-mercaptoethanol ...
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bioRxiv - Immunology 2023Quote: ... color prestained protein standard (New England Biolabs GmbH, Germany) was included on each gel ...
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bioRxiv - Biophysics 2023Quote: ... The sample was phosphorylated using protein kinase A (NEB) and then further purified on size exclusion chromatography.
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bioRxiv - Developmental Biology 2023Quote: ... Synthesized sgRNA was mixed with Cas9 protein (NEB# M0646T) just before microinjection into zebrafish embryo.
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bioRxiv - Biophysics 2023Quote: ... Proteins were cleaved with TEV protease (New England Biolabs) and further purified via reverse IMAC ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were subsequently purified on amylose resin (E8021L, NEB): 3 ml resin per column and 45 ml of extract ...
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bioRxiv - Neuroscience 2023Quote: ... Protein A beads (10 µl, New England Biolabs, #S1425) were washed twice in coIP-solution and consecutively incubated for 1 hour at 4°C with an anti-synaptopodin antibody (rabbit anti-Synaptopodin ...
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bioRxiv - Cell Biology 2024Quote: ... RRID:Addgene_119754).CAPS-GST protein was expressed in BL21Star bacteria (NEB); the protein was isolated by binding to glutathione-Sepharose (Pierce ...
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bioRxiv - Neuroscience 2024Quote: ... 25-50 ul Protein G Magnetic Beads (NEB #S1430S) were incubated with 3ug GFP (D5.1 ...
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bioRxiv - Cell Biology 2022Quote: ... and protein samples and molecular weight markers (PageRuler Plus Pre-stained Protein Ladder, ThermoFisher Scientific 26619, or Colour pre-stained protein standards NEB P7712 or P7719) were separated using 8% ...
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bioRxiv - Cell Biology 2023Quote: ... 10 % SDS-PAGE gel was prepared and 50 μg of protein lysate was loaded along with the protein standard ladder (Cat.No.: P7719S, New England Biolabs, Ipswich, Massachusetts, United States), and electrophoresis was performed for 1.5 hr in ice-cold 1x Laemmli electrophoresis running buffer ...
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bioRxiv - Biophysics 2020Quote: For the insertion of an ATTO647N-labeled oligonucleotide complementary to the position 14711 bp from the biotinylated end of the λ DNA we employed the previously described strategy14 and followed the more recently described procedure.15 2 μg of λ DNA was incubated for 2 hours with the nicking enzyme Nt.BstNBI (20 units, NEB) at 50 °C in the nickase buffer ...
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bioRxiv - Neuroscience 2021Quote: ... PCR product (2 μg) and pEYFP-C1 vector DNA (2 μg) were digested with BamHI-HF and HindIII-HF (New England Biolabs) according to the manufacturer ...
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bioRxiv - Synthetic Biology 2021Quote: ... The annealing was carried out in 96-well PCR plates by mixing 2 μL of each oligonucleotide at 100 μM with 2 μL of 10x T4 DNA Ligase Reaction Buffer (NEB) and 14 μL of water ...
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bioRxiv - Systems Biology 2021Quote: ... PCR reactions with plasmid and genomic DNA templates were performed using the Phusion High-Fidelity 2× Master Mix or Q5 High-Fidelity 2× Master Mix (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... was amplified and barcoded in parallel using the same PCR program in 50 μL PCR reaction (2 μL gap-repaired DNA, 25 μL 2× NEBNext High-Fidelity PCR Master Mix (NEB), 1.5 μL 10 μM i5 universal PCR primer ...
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bioRxiv - Synthetic Biology 2023Quote: ... we first mixed 10.5 uL of purified RNA with 2 uL of 2 uM RT primer (AGGGACATCGTAGAGAGTCGTACTTANNNNNNNNNNAGATGAACTTCAGGGTCAGC, where Ns comprise the UMI) and 2uL of 10mM dNTP mixture (NEB), incubated the mixture at 65C for 5 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... A repair template for editing endogenous plk-2 gene to express PLK-2::3xFLAG::TIR1CIP was assembled into a pCR-Blunt backbone using Gibson Assembly (NEB) and confirmed using Sanger sequencing and whole-plasmid Nanopore sequencing (Primordium) ...
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bioRxiv - Molecular Biology 2021Quote: ... 41.7 µL of Phusion 2× Master Mix (NEB), 2.5 µL of 10 µM primer mix (515F forward and 806R reverse rRNA gene V4 primers with Illumina MiSeq adaptors) ...
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bioRxiv - Genomics 2019Quote: ... 10 μl NEB Buffer 2 (New England Biolabs), 12 μl dNTP mix (2.5 mM) ...
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bioRxiv - Cell Biology 2019Quote: ... 2 mM ribonucleoside vanadyl complex (RVC) (NEB, S1402), 0.02% RNase-free BSA (Ambion ...
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bioRxiv - Evolutionary Biology 2020Quote: ... treated with 2 units of DNase I (NEB) at 37°C for 1 hour followed by DNase I heat inactivation ...
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bioRxiv - Genomics 2020Quote: ... 2 μl Antarctic Phosphatase [5k U/mL] (NEB) and 1 μl RNase inhibitor and incubating at 37 °C for 30 minutes with shaking ...
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bioRxiv - Genomics 2021Quote: ... 10 µL of 2% BSA (New England Biolabs), 930 µL of nuclease-free water and supplemented with 0.2 U/ul RNaseIN Plus (Promega ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 µl RNase Inhibitor (80U final, NEB M0307L) and 2 µl SuperScript III (400U final ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 µl RNase Inhibitor (80U final, NEB M0307L) and 1 µl AMV RT (12U final ...
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bioRxiv - Genetics 2021Quote: ... viewpoint 2: 10X NEBuffer™ DpnII (R0543M, NEB)) and 15 μl of 10% SDS buffer were added to the 450 μl sample ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... 2 μl 10x CutSmart buffer (New England Biolabs), 10 μl shrimp alkaline phosphatase (New England Biolabs) ...
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bioRxiv - Microbiology 2021Quote: ... 2 μl 10× T4 RNA ligase buffer (NEB), 20 U T4 RNA ligase enzyme ...
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bioRxiv - Microbiology 2021Quote: ... 2 μl of 10× Antarctic Phosphatase buffer (NEB), 1 U Antarctic Phosphatase ...
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bioRxiv - Neuroscience 2022Quote: ... and 2 µl T4 ligase (NEB cat #M0202M). For Capture-C ...
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bioRxiv - Molecular Biology 2022Quote: ... and 2 μl T4 Ligase Buffer (NEB, B0202) in a total volume of 30 μl for 18 hours at 16 °C ...
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bioRxiv - Immunology 2022Quote: ... dNTP mix (2 mM each) (New England Biolabs), 50U Superscript III RTase ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 units of T4 polynucleotide kinase (NEB, M0201S), 0.09 nM dNTPs and 0.045 μg/μL of BSA at 12°C for 30 min ...
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bioRxiv - Plant Biology 2021Quote: ... M-TM2^2 was digested with BslI (NEB) for 3 hours at 55°C and M-2 was digested with XhoI (NEB ...
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bioRxiv - Biophysics 2021Quote: ... 2 nM M13mp18 circular ssDNA (New England Biolabs) (Table S3 ...
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bioRxiv - Synthetic Biology 2022Quote: ... added Cut smart (2 ul, Cat # B7204; NEB), Bsa I (2ul ...
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bioRxiv - Microbiology 2022Quote: ... 2 μl of RNaseH (10 U, NEB, M0297) was added followed by an incubation of 20 minutes at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 μl 10x NEBuffer 1 (New England Biolabs), 2 μl 50 mM MnCl2 ...