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Citations for New England Biolabs :
551 - 600 of 1359 citations for F actin uncapping protein LRRC16A CARMIL1 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Protein A magnetic beads (New England Biolabs, Ipswich, MA, USA) were then added to the cell lysate and antibody mixture ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant hACE2 protein was digested using EndoH (New England Biolabs) and thrombin (Sigma-Aldrich ...
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bioRxiv - Genetics 2022Quote: ... and 2 μL of lambda protein phosphatase (NEB, Catalog #P0753S) were added to the washed FRQ-coupled V5 resin ...
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bioRxiv - Microbiology 2022Quote: ... the PURExpress In Vitro Protein Synthesis Kit (New England Biolabs) was used to translate the synthetic transcriptome described above ...
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bioRxiv - Microbiology 2022Quote: ... A pre-stained protein standard (New England Biolabs, MA, USA) was used to estimate molecular weight ...
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bioRxiv - Microbiology 2024Quote: ... 50 μL of protein G beads (New England Biolabs (NEB), S1430S ...
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bioRxiv - Microbiology 2024Quote: ... 50 μL of protein G beads (New England Biolabs (NEB), S1430S ...
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bioRxiv - Cell Biology 2024Quote: ... STAT1 proteins were dephosphorylated by lambda phosphatase (NEB, Ipswich MA) followed by the addition of protease and phosphatase inhibitors (Thermo Fisher) ...
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bioRxiv - Plant Biology 2023Quote: ... and Lambda Protein Phosphatase (Lambda PP) was purchased from NEB. After kinase reaction in kinase buffer ...
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bioRxiv - Biochemistry 2023Quote: ... The proteins were expressed in BL21(DE3) (New England Biolabs) in LB grown in 2 L baffled shake flasks ...
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bioRxiv - Microbiology 2023Quote: ... proteins were removed using the Monarch RNA Cleanup Kit (NEB) and 5’-mono-phosphorylated v51_mut_S templates were specifically digested with a terminator 5’-phosphate-dependent exonuclease (Biosearch technologies ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The unbound fraction was dephosphorylated using lambda protein phosphatase (NEB), calf intestinal phosphatase (NEB) ...
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bioRxiv - Cell Biology 2023Quote: ... kit and co-injected with Cas9 protein (New England Biolabs) into one-cell stage zebrafish eggs ...
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bioRxiv - Cell Biology 2023Quote: ... in the presence of protein kinase buffer (New England Biolabs) with 3 μCi [γ-32P]ATP ...
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bioRxiv - Biochemistry 2023Quote: ... and 5 µl of prestained protein ladder (New England Biolabs). The gel was electrophoresed at 150 V ...
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bioRxiv - Plant Biology 2024Quote: ... 400 U Lambda Protein Phosphatase (New England Biolabs, Frankfurt, Germany) in NEBuffer ...
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bioRxiv - Microbiology 2024Quote: Experiments with PURExpress in vitro protein synthesis kit (NEB, E6800) were performed as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: Experiments with PURExpress In Vitro Protein Synthesis Kit (NEB, E6800) were performed as per the manufacturer’s instructions using 10 ng/µL of DHFR-Strep reporter plasmid with the addition of 0.8 U/µL RNase Inhibitor Murine (NEB ...
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bioRxiv - Neuroscience 2024Quote: ... The mix consisted of 1 µL Cas9 protein (BioLabs, M0369M), 1 µL Fast Green FCF dye (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... supplemented with extreme thermostable ssDNA-binding protein (ET SSB, NEB), under the manufacturer’s recommended reaction conditions (1X standard Taq reaction buffer ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were purified on pre-equilibrated Ni-NTA beads (NEB) and exchanged into a protein storage buffer (10 mM HEPES-KOH pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... The proteins were expressed in BL21(DE3) (New England Biolabs) in Luria Broth (10 g Tryptone ...
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bioRxiv - Genetics 2024Quote: ... Injection solution contained 300 ng/μL spyCas9 protein (NEB, USA), 100 ng/μL kmo-sgRNA ...
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bioRxiv - Developmental Biology 2024Quote: ... together with 2 µM of Cas9 protein (NEB, Cat# M0646T). Tol2-plasmids were diluted in 0.05% Phenol red to a final concentration of 100 ng/µL ...
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bioRxiv - Cancer Biology 2024Quote: ... Bound antibodies were detected by incubation with DyLight 800-conjugated secondary antibodies (New England BioLabs), and analysed using an InnoScan 710-IR scanner (Innopsys) ...
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bioRxiv - Cell Biology 2023Quote: ... The protein dephosphorylation was performed on total WT and MLP knockout heart lysates by using Alkaline Phosphatase (rSAP)(M0371S NEB, 1U of rSAP/10mg of protein). The protein lysate containing phosphatases ...
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bioRxiv - Microbiology 2020Quote: ... The soluble proteins were mixed with amylose resin (New England Biolabs) according to the manufacturer’s protocols.
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bioRxiv - Molecular Biology 2020Quote: The protein was expressed in NiCo21 (DE3) cells (New England Biolabs) grown in Overnight Express Instant TB Medium (EMD Millipore ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with DTT and proteins were degraded using proteinase K (NEB). Total cellular RNA was purified using the RNeasy maxi kit (QIAGEN ...
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bioRxiv - Developmental Biology 2021Quote: ... Cas9 protein (New England Biolabs, #M0646M, EnGen Cas9 NLS 20 μM) and 300 pg of sgRNAs along with 5 ng of GFP cRNA were co-injected into the X ...
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bioRxiv - Developmental Biology 2021Quote: ... in equimolar ratios and EnGen®Spy Cas9 NLS protein (NEB) into 1-cell zebrafish embryos.
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bioRxiv - Immunology 2021Quote: ... Deglycosylation was performed on beads using Protein Deglycosylation Mix II (NEB) in denaturing conditions according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... associated with EnGen™ Spy Cas9 NLS protein (New England BioLabs), using the 4D-Nucleofector™ System (Lonza ...
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bioRxiv - Physiology 2020Quote: ... were co-injected with 0.5 μl Cas9 protein (NEB, M0386, 20μM), diluted 1:10 ...
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bioRxiv - Cell Biology 2021Quote: ... Molecular weights were identified using a protein standard (BioLabs, Cat# P7712S). Beta-actin was used as the loading control ...
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bioRxiv - Bioengineering 2021Quote: Recombinant RfxCas13d proteins were expressed in E.coli NiCo21(DE3) (NEB C2529). Cells were grown in 1L of lysogeny (Luria-Bertrani ...
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bioRxiv - Neuroscience 2021Quote: ... 1 µl of lambda protein phosphatase (New England BioLabs, Ipswich, MA). Each mixture was incubated at 30 °C for either 1 ...
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bioRxiv - Plant Biology 2020Quote: ... the purified proteins (0.125 mg) or RNase If (1.25 U; NEB) were incubated at 25°C for 60 minutes with a fluorescent-labeled RNA substrate (5’-fluorescein isothiocyanate (FITC)-CUUUUAG(A20) ...
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bioRxiv - Molecular Biology 2022Quote: ... and α1-3,4 fucosidase (New England Biolabs, 4 U/μg protein). All enzymatic reactions were performed as a 1-step reaction with 1x Glycobuffer 2 (New England Biolabs) ...
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bioRxiv - Microbiology 2020Quote: ... Molecular weights were determined using protein standards (New England Biolabs P7719).
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bioRxiv - Molecular Biology 2021Quote: ... The protein was then digested with LysC endoproteinase (0.25 µg; NEB) for 4 hours at 37°C ...
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bioRxiv - Bioengineering 2020Quote: IVPS was performed using PURExpress In Vitro Protein Synthesis Kit (NEB) with supplements (16 U RNase inhibitor (NEB) ...
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bioRxiv - Pathology 2021Quote: ... Soluble protein extracts were purified using amylose resin (New England Biolabs) and/or Ni-NTA agarose beads (Life Technologies).
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bioRxiv - Microbiology 2020Quote: ... Magnetic beads conjugated with IgG Protein A/G (New England Biolabs) were pre-blocked with 0.5 mg/mL of sonicated salmon sperm DNA (Thermo Scientific ...
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bioRxiv - Biophysics 2021Quote: ... 100μg/ml single stranded DNA binding protein (gp32) from T4 (NEB), 277μg/ml T4 DNA clamp ...
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bioRxiv - Biochemistry 2021Quote: ... the purified proteins were incubated with excess streptavidin (New England Biolabs) for 10 minutes before analysis via gel-shift using SDS-PAGE ...
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bioRxiv - Developmental Biology 2021Quote: ... Cas9 protein (2.12 ng) (New England BioLabs Inc. Ipswich, MA, USA,) and gRNA (133 pg ...
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bioRxiv - Developmental Biology 2021Quote: ... Purified sgRNA and Cas9-NLS protein (New England Biolabs® Inc.) were diluted to 300ng/ul.
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bioRxiv - Molecular Biology 2021Quote: ... Samples were then added to protein A magnetic beads (NEB, S1425S) and rotated at 4°C for 4 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... and proteins were purified using amylose resin column (New England Biolabs). Fractions were eluted and checked by silver staining ...