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Citations for New England Biolabs :
551 - 600 of 1440 citations for 6 Cyclohexylcarbamoylcyclohex 3 enecarboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... and poly(A)-tailed at their 3’ ends with poly(A) polymerase (NEB), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... We ligated a 3’ adapter ligation using T4 RNA Ligase 1 (NEB, M0204L). We performed a second bead binding followed by a 5’ decapping with RppH (NEB ...
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bioRxiv - Genomics 2022Quote: ... then perform A-tailing with Klenow Fragment (3’-> 5’ exo-) (NEB, Cat. M0212S) provided with dATP ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was removed 3’phosphoryl groups using T4 Polynucleotide Kinase (New England Biolabs) in the buffer (70 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 ng of DNA was used per qPCR reaction (qPCR Master Mix, NEB). PCR was performed using an Applied Biosystems 7500 instrument (software version 2.3) ...
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bioRxiv - Genomics 2019Quote: ... and 3 µl of NEBNext Ultra II End Prep Enzyme Mix (E7545L, NEB) were mixed gently in 1.5 ml Eppendorf tube ...
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bioRxiv - Molecular Biology 2021Quote: ... 5’ phosphorylation and 3’ dephosphorylation were performed with T4 PNK (NEB, Cat: M0201S) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and A-tailed using Klenow 3’-5’exo-enzyme (NEB, Cat. No. M0212). Illumina sequencing library adapters were subsequently ligated to DNA ends ...
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Glucocorticoid receptor collaborates with pioneer factors and AP-1 to execute genome-wide regulationbioRxiv - Genomics 2021Quote: ... In-line barcoded 3′ adapters were ligated with T4 RNA ligase I (NEB) for 12 hours at 20°C ...
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bioRxiv - Genomics 2022Quote: ... and 3’-adenylated with NEBNext Ultra II End Repair/dA-Tailing Module (NEB). The DNA was then purified with AMPure XP beads (Beckmann Coulter ...
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bioRxiv - Molecular Biology 2022Quote: ... pUC19-3’ITR-3xCTCF- rHA was linearized with KpnI and HindIII (NEB, R3104L) and the Neomycin resistance gene was amplified from pEN113 (Addgene ...
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bioRxiv - Genomics 2022Quote: ... and 3 µL 5U/µL DNA Polymerase I Large (Klenow) Fragment (NEB, #M0210V) was added and incubated at 37°C for 45min with rotation ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 mM MgCl2 and 2 μL (10 U) RNase H (NEB, Cat# M0297S) in order to digest poly(A ...
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bioRxiv - Molecular Biology 2022Quote: ... Next the 3’ primer regions were truncated using the BciVI restriction endonuclease (NEB), followed by 2.0x AMPure XP cleanup ...
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bioRxiv - Molecular Biology 2023Quote: Hi-C libraries were treated with 3 U of T4 DNA polymerase (NEB) in the presence of 0.1 mM dGTP (NEB ...
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bioRxiv - Genomics 2023Quote: ... and A-tailed with Klenow Fragment (3’-to-5’ exo-, New Englands Biolabs) following manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... mRNA was radiolabelled at the 3’ end using T4 RNA ligase I (NEB) and [32P] pCp (Perkin Elmer ...
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bioRxiv - Cell Biology 2023Quote: ... which were then degraded by the 5’-3’ ssDNA exonuclease RecJ (NEB, M0264S). After rRNA reduction using the riboPOOL rRNA depletion kit (siTOOLs Biotech ...
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bioRxiv - Genomics 2023Quote: ... Nicked DNA was then extended with 3 U of Vent (exo-) Polymerase (NEB), 100-300 µM dNTPs ...
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bioRxiv - Genetics 2024Quote: ... the slides were treated with 3 U/µl exonuclease III (New England Biolabs) in 1x NEB cutsmart buffer and incubated at 37°C for 15 min to digest nicked BrdU-positive strands ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA was then ligated to adaptors at the 3’ end by NEB 3’ SR adaptor and 5’ end by T4 RNA ligase ...
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bioRxiv - Molecular Biology 2021Quote: ... 6×50 µl PCR reactions were set up using NEBNext Ultra II Q5 master mix (New England Biolabs) with 5 nM oligos as template ...
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bioRxiv - Genetics 2022Quote: ... we PCR amplified NatMX from Addgene plasmid #35121 with the primers listed in Supplementary Table 6 using Phusion Hot Start Flex DNA polymerase (NEB). The NatMX cassette was transformed into the BY strain using the methods described above and transformants were plated onto YPD medium containing clonNAT ...
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bioRxiv - Molecular Biology 2022Quote: ... The final cDNA libraries were amplified (cycle number of 6) using NEBNext Multiplex Oligos for Illumina (NEB, E7335) and purified using SPRIselect size selection beads (Beckman Coulter ...
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bioRxiv - Biochemistry 2023Quote: ... and transformed 1 µL of the reaction mixture into 6 µL of BL21 competent cells (New England Biolabs). After heat shock and recovery in SOC media ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.6 mM of A, C, and T, NEB-N0446S; 0.1 µM Clean.G; 800 U/ml Bst Polymerase, NEB-M0374L ...
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bioRxiv - Genetics 2020Quote: Total RNA was extracted using the standard hot acid phenol method and treated with DNase 1 (New England Biolabs). qRT-PCR was performed using amfiRivert cDNA synthesis Platinum Master Mix from Gendepot ...
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bioRxiv - Biochemistry 2021Quote: ... The alkyne-PAR was purified to remove excess alkyne-PEG1-amine using the Monarch Nucleic Acid Cleanup Kit (NEB) following the recommended protocol ...
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bioRxiv - Biophysics 2019Quote: ... 115 amino acids) fused to the C-terminus of maltose binding protein (MBP) in pMALX vector (New England Biolabs), MBP-5-HT3A-ICD-pMALX ...
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bioRxiv - Biochemistry 2021Quote: ... 70 and 80 amino acids deletion mutants were carried out with the Q5 mutagenesis kit (New England Biolabs, MA). All AtAtm3 constructs were overexpressed in Escherichia coli BL21-gold (DE3 ...
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bioRxiv - Synthetic Biology 2022Quote: Genomic DNA was purified from single-humanized Hsα1 and quintuple-humanized Hsα1,α2,α3,α4,α7 strains using the Monarch Nucleic acid purification kit according to the manufacturer’s protocol (NEB). Spheroplasts were obtained before the genomic DNA extraction for a high-quality DNA prep ...
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bioRxiv - Biochemistry 2023Quote: ... Single or multiple amino acid mutations in full-length GPR161 were generated using Q5 site-directed mutagenesis kit (NEB).
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bioRxiv - Biochemistry 2023Quote: ... The extracted plasmid DNA was reconstituted in cacodylic acid (10 mM, pH 6.5) and subjected to linearization by HINDIII-HF restriction digestion (New England Biolabs). Following digestion ...
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bioRxiv - Biophysics 2023Quote: ... Single-amino acid exchanges were generated by Q5® site-directed mutagenesis (New England Biolabs GmbH, Frankfurt am Main) using the appropriate primer pairs which were designed by using NEBaseChanger ...
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bioRxiv - Microbiology 2023Quote: 20 ng of nucleic acid was enriched for viral genomic sequences using NEBNext Microbiome DNA Enrichment Kit (NEB, E2612) to reduce host genomic DNA contamination ...
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bioRxiv - Cell Biology 2020Quote: ... 3 µg of purified DNA was digested with 10 units I-SceI (NEB, R0694), 10 units I-CeuI (NEB ...
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bioRxiv - Genomics 2020Quote: ... and followed by incubation with 3 μl (1 U/μl) of USER enzyme (NEB) at 37°C for 20 min ...
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bioRxiv - Neuroscience 2021Quote: ... with 3’-O-Me-m7GpppG anti-reverse cap analog (ARCA) or ApppG cap (NEB) added at 8:1 to GTP for a capping efficiency of ∼90% ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 μg of RNA was resuspended in 6x Loading dye (Biolabs, cat. No B7024S) and separated on a 20% PA gel without urea run at 160 volts at 4°C ...
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bioRxiv - Genomics 2020Quote: ... 2 μl 10 mM dATP and 5 μl Klenow Fragment (3’ −> 5’ exo-) (NEB) for A-tailing at 37°C for 30 min ...
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bioRxiv - Molecular Biology 2019Quote: ... Precipitated RNA 3’ends were first dephosphorylated by shrimp alkaline phosphatase (SAP) (NEB M0371), and 3’ RNA linker (20 µM ...
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Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: ... 5’-gaattcgatgtgtaggctggag-3’ using Gibson assembly technique (kit was purchased from New England Biolabs), to yield pIJ773-Δspt9 ...
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bioRxiv - Cancer Biology 2019Quote: ... KRAS and BRAF 3’ homology arms using high-fidelity Phusion Polymerase (New England BioLabs). The 5’ homology arm of KRAS spans the region Chr12:25245385-25245994 ...
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bioRxiv - Plant Biology 2020Quote: ... adaptor-ligated fragments were treated with 3 µl of USER® Enzyme (NEB, USA) before selective PCR enrichment using Phusion® High-Fidelity DNA polymerase using Universal and index primers ...
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bioRxiv - Plant Biology 2021Quote: ... and ligated at a 3:1 amplicon:plasmid ratio with t4 DNA ligase (NEB, US) overnight at 16°C ...
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bioRxiv - Plant Biology 2021Quote: ... for 3 hours at 55°C and M-2 was digested with XhoI (NEB) overnight at 37°C ...
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bioRxiv - Biophysics 2020Quote: ... and 7U/mL Klenow Fragment of DNA Polymerase I (3’-5 exo-) (NEB, M0212). Reactions were incubated at 37°C and incorporation of labeled dCMP was monitored by an acid precipitation assay ...
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bioRxiv - Genetics 2022Quote: ... and ligating into the BbsI ligated U6:3 vector (T4 ligase NEB, BbsI NEB) (S3 Table) ...
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bioRxiv - Genetics 2022Quote: ... and ligating into the BbsI ligated U6:3 vector (T4 ligase NEB, BbsI NEB) (S3 Table) ...
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bioRxiv - Microbiology 2022Quote: ... 15 μl 20% SDS and 3 μl 20 mg/ml Proteinase K (NEB #P8107) was added to the sample and the mixture was incubated at 37 °C for 30 min with agitation at 1400 rpm to completely lyze the cells ...