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Citations for New England Biolabs :
551 - 600 of 6189 citations for 1 3 Bis 2 4 hydroxyphenyl 2 propyl benzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2022Quote: ... and assembled into the backbone vector at a 2:1 molar ratio via Golden Gate Assembly81 (NEB #R3733). The assembly mix was then transformed into electrocompetent DH10B E ...
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bioRxiv - Cell Biology 2023Quote: ... 100 mM NaCl, 2 mM EDTA, 1% NP40, 0.1%SDS, 0.5 mM DTT, 40 U RNase inhibitor [New England Biolabs, cat#M0314L] ...
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bioRxiv - Bioengineering 2024Quote: ... DNA fragments encoding CDRs 1 and 2 were digested overnight with BsaI-HFv2 and BbsI-HFv2 respectively (NEB). Reactions were cleaned up with Macherey Nagel Gel cleanup columns ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 µL of each reaction was combined with 2 µL of 6X Purple Gel Loading Dye (NEB B7024S) and 11 µL H2O and run on a 1.2% agarose gel containing 1X GelGreen Nucleic Acid Stain (Biotium 41005 ...
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bioRxiv - Microbiology 2022Quote: ... 1 μl of methylation-sensitive restriction enzyme DpnI and 2 μl CutSmart® buffer (both from NEB inc.) were added to the assembled reaction and incubated at 37 °C for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: We then set up the digestion reaction (1.5 μg of pX459, 2 μL of 10X NEBuffer 2.1, 1 μL of BbsI (NEB), and added H2O to a final volume of 20 μL) ...
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bioRxiv - Genomics 2024Quote: ... were assembled by Gibson assembly using a 2:1 insert:vector ratio with Gibson Assembly Master Mix (NEB E2611S). Assemblies were transformed into NEB 5-alpha E.coli competent cells and single colonies were picked and sequence validated by Sanger sequencing ...
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bioRxiv - Genomics 2021Quote: ... 5 μl of 100 ng/μl cDNA diluted from the previous step was combined with 2 μl block mix and 2 μl nuclease free water (NEB, catalog no. AM9937), then the cDNA-block oligo mix was incubated on a thermocycler under the following condition to allow block oligo mix to bind to 5’ and 3’ end of the cDNA molecule ...
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bioRxiv - Evolutionary Biology 2024Quote: ... was prepared by combining 2 µL of 100 µM guide RNA (gRNA) with 2 µL of 20 µM EnGen SpyCas9 NLS (NEB, Cat. No. M0646T), and incubated at room temperature for 2-4 hours ...
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bioRxiv - Cell Biology 2023Quote: ... 250 μL of 2 mg mL-1 Y289L GalT,46 50 μL of 500 kU mL-1 PNGase F (New England Biolabs, 5 U μg-1 of protein), and 62.5 μL of Lambda Protein Phosphatase (New England Biolabs ...
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bioRxiv - Immunology 2023Quote: ... We then used a 2:1 insert to vector ratio in a 1 hour Hifi assembly reaction using NEBuilder HiFi DNA Assembly kit (NEB, Cat. No. E2621). The Hifi assembly products were Ampure bead purified and eluted into 20 µL of H2O ...
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bioRxiv - Microbiology 2023Quote: ... We then used a 2:1 insert to vector ratio in a 1 hour HiFi assembly reaction using NEBuilder HiFi DNA Assembly kit (NEB, Cat. No. E2621). HiFi assembly products were Ampure bead purified and eluted into 20uL of H2O for higher electroporation efficiency.
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bioRxiv - Molecular Biology 2021Quote: ... 5 µL of linker ligation mixture was added (38% PEG-8000, 1x NEB T4 ligase buffer, 2 µM oBZ407_preA preadenylated linker, 100 units NEB T4 RNA ligase 2, truncated) and they were further incubated at 37 °C for 3 hrs ...
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bioRxiv - Microbiology 2020Quote: ... then quenched with 2 μL 6X purple loading dye (NEB) at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 µL of 10x uracil DNA glycosylase buffer (NEB M0280S), 2.5 µL RNase A (M0280S ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 units of 2’-O-Me-transferase (New England Biolabs) and 25 units RNasin Plus RNase inhibitor (Promega) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 200 U/uL of T4 RNA Ligase 2 (NEB M0351S), 1X T4 RNA Ligase Reaction Buffer (NEB B0216L) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 2 uL of 10X T4 RNA ligation buffer (NEB B0216L), 2 uL of 10mM ATP ...
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bioRxiv - Developmental Biology 2020Quote: ... a mix containing 2 μl of RNAse H (NEB, #M0297S), 1 μl of E ...
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bioRxiv - Genomics 2020Quote: ... 2 μL of NEB buffer 3.1(1X, New England Biolabs) and 1 μL of each DNA barcode B and ligation linker mix (B1-B50 ...
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bioRxiv - Microbiology 2022Quote: ... PEG 8000 (17.5% final) and T4 RNA ligase 2 (NEB) in 1x T4 RNA ligase buffer at 25°C for 1 h ...
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bioRxiv - Molecular Biology 2021Quote: ... and 0.5 units/µl T4 RNA Ligase 2 (NEB, M0239S), with the enzyme added after the other components had been mixed and incubated at 23°C for 5 min ...
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bioRxiv - Genomics 2020Quote: ... 2 μL of NEB DNA Quick Ligase (New England Biolabs) and 3 μL of Illumina Indexed adapter were added to the beads and incubated for 15 minutes at room temperature ...
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bioRxiv - Genomics 2020Quote: ... 12.5 μL NEBNext High-Fidelity 2× PCR Master Mix (NEB) were added and samples were PCR-amplified (72 °C 5 min ...
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bioRxiv - Genomics 2020Quote: ... 2× LongAmp Taq Master Mix (25 μl; New England Biolabs) was used to synthesize second-strand cDNA using 2 μl of the PR2 primer (ONT) ...
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bioRxiv - Genomics 2021Quote: ... 12.5 µL NEBNext High-Fidelity 2× PCR Master Mix (NEB) were added and samples were PCR-amplified (72 °C 5 min ...
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bioRxiv - Genomics 2021Quote: ... 12.5 µL NEBNext High-Fidelity 2× PCR Master Mix (NEB) were added to each well and samples were PCR-amplified (72 °C 5 min ...
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bioRxiv - Microbiology 2021Quote: F(ab’)2 fragments were generated using IdeZ protease (NEB), purified using CaptureSelect LC-lambda affinity matrix (human ...
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bioRxiv - Neuroscience 2020Quote: ... 12.5 µL NEBNext High-Fidelity 2× PCR Master Mix (NEB) were added and samples were PCR-amplified (72 °C 5 min ...
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bioRxiv - Microbiology 2020Quote: ... 2 U of HindIII (New England Biolabs, Beverly, MA, USA), 2 U of EcoRI (New England Biolabs ...
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bioRxiv - Systems Biology 2021Quote: ... PCR amplicons were purified from a 2% agarose gel (NEB), digested with EcoRI-HF and NotI-HF (NEB) ...
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bioRxiv - Cell Biology 2021Quote: ... diluted 2-fold in 1x G3 buffer (New England Biolabs) and incubated with 160 U endoglycosidase H (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: ... and 2 μL T4 DNA Polymerase (#M0203S, New England Biolabs). The reaction was incubated at room temperature for 45 min with gentle mixing every 5 min ...
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bioRxiv - Microbiology 2021Quote: ... 2 µl of 20 µM SpCas9 (NEB, Cat. No. M0646M) was placed in the bottom of a 0.25 ml PCR tube ...
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bioRxiv - Neuroscience 2020Quote: ... 12.5 μL NEBNext High-Fidelity 2× PCR Master Mix (NEB) were added and samples were PCR-amplified (72 °C 5 min ...
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bioRxiv - Cell Biology 2021Quote: ... Clarified lysates were flowed over 2 mL amylose resin (NEB) equilibrated with MBP lysis buffer to capture MBP-tagged protein ...
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bioRxiv - Cell Biology 2021Quote: ... Then 2-5 unites of RNase H (New England Biolabs) in 5 µl of 1x RNase H buffer were added to the annealed RNA-chimeric oligo mixture and the RNase H cleavage reaction was performed at 37o C for 1 h ...
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bioRxiv - Molecular Biology 2022Quote: ... α2-3,6,8 neuraminidase (New England Biolabs, 25 U/μg protein) and α-N-acetyl-galactosaminidase (New England Biolabs ...
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bioRxiv - Synthetic Biology 2022Quote: ... 2 µl of T4 DNA Ligase Buffer (New England Biolabs), 1 µl of T4 or T7 DNA Ligase (New England Biolabs) ...
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bioRxiv - Synthetic Biology 2022Quote: ... 2 µl of T4 DNA Ligase Buffer (New England Biolabs), 1 µl of T4 or T7 DNA Ligase (New England Biolabs ...
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bioRxiv - Biophysics 2022Quote: ... 2 μL (1000 U) PNGase F (New England Biolabs Inc) were added and the reaction was incubated for 6 h at 37 °C ...
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bioRxiv - Biophysics 2022Quote: ... and 2 µM of SNAP-Surface Alexa Fluor 647 (NEB) in extracellular buffer for 30 minutes at 37 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... 100 units of 2′-O-Me-transferase (New England Biolabs) and 25 units RNasin Plus RNase inhibitor (Promega) ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 μl of 2.5 mM dNTP (New England Biolabs N0447S), 0.75 μl of 50 mM MgCl2 ...
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bioRxiv - Immunology 2022Quote: ... 2 mM ribonucleoside vanadyl complexes (New England Biolabs, Ipswich, MA), 0.02% ribonuclease-free bovine serum albumin (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2022Quote: ... 10x T4 DNA ligase buffer (2 ul; Cat # B0202S; NEB), Bsa I (1ul ...
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bioRxiv - Microbiology 2022Quote: ... 2 μl 10× T4 RNA ligase reaction buffer (NEB, M0242), 10 μl 50% PEG 8000 (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 μl T4 PNK (NEB, cat#M0201S, 10 U/μl), 1.5 μl T4 DNA Polymerase (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 μl T4 PNK (NEB, cat#M0201S, 10 U/μl) and 1.5 μl Klenow Fragment (3’->5’ exo- ...
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bioRxiv - Plant Biology 2020Quote: ... 2 µl of Taq 5× Master Mix (New England Biolabs) and Milli-Q water ...