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Citations for New England Biolabs :
5801 - 5850 of 9358 citations for Rat Leptin RIA kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... cDNA fragments were then constructed into libraries using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645). 100M reads/read pairs per library were requested ...
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bioRxiv - Plant Biology 2023Quote: ... ChIP DNA libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... This was followed by two sets of enzymatic conversion steps using the Enzymatic Methyl-seq kit (NEB, Cat# E7120L) to differentiate cytosines from 5mC and 5hmC ...
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bioRxiv - Neuroscience 2023Quote: ... followed by preparation of RNA libraries using the NEBNext Ultra RNA Prep kit (NEB #E7530) (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Neuroscience 2023Quote: ... followed by preparation of RNA libraries using the NEBNext Ultra RNA Prep kit (NEB #E7530) (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Neuroscience 2023Quote: ... Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (#E7530L, NEB, USA) following the manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA-seq libraries were made using the NEBNext Ultra II Directional RNA Library Prep kit for Illumina (NEB, #E7760) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: Libraries in this paper were prepared using NEB’s rRNA Depletion Kit (Bacteria) for rRNA removal with beads (NEB #E7860). Post rRNA removal samples were prepared for either Illumina or Singular Sequencing using NEBNext Ultra II RNA Library Prep Kit for Illumina with Beads (NEB#E7775) ...
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bioRxiv - Genomics 2023Quote: ... Linearized destination vectors were gel purified using 0.7% agarose gels and extracted with the Monarch DNA Gel Extraction Kit (New England Biolabs), before further purification by isopropanol precipitation.
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bioRxiv - Genomics 2023Quote: Sample DNA concentrations were quantified using Qubit and libraries prepared using NEBNext Ultra II DNA library preparation kit (NEB) according to the manufacturer’s instructions with the following modifications ...
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bioRxiv - Genomics 2023Quote: ... Total (“steady-state”) RNA was converted to sequencing libraries using NEB polyA capture kit (product no) followed by NEB Ultra directional II RNA-seq library kits ...
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bioRxiv - Genomics 2023Quote: ... or 100 cGy) was processed for bisulfite sequencing library construction using NEBNext Ultra II DNA Library Prep Kit (NEB) along with methylated and indexed NEXTflex Bisulfite-seq Barcodes (Bio scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... An RNA-seq library was prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Sequencing libraries were generated using the NEBNext Ultra™ DNA Library Prep Kit for Illumina (NEB, Ipswich, MA, USA) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... RNA sequencing library preparation was performed utilizing the NEBNext Ultra RNA Library Prep Kit for Illumina by following the manufacturer’s recommendations (NEB). Sequencing libraries were validated on the Agilent 2100 Bioanalyzer System (Agilent Technologies ...
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bioRxiv - Microbiology 2023Quote: ... NEBNext® UltraTM II Non-Directional RNA Library Prep Kit for Illumina® (New England BioLabs Inc., MA, USA) was used for the remaining steps of library construction ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequencing libraries were generated using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, UK) following manufactures’ protocol ...
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bioRxiv - Microbiology 2023Quote: ... Removal of rRNA from the samples was carried out using NEBNext Ultra directional RNA library prep kit (NEB, MA). Sequencing libraries were generated using the NEBNext Ultra RNA library prep kit for Illumina (NEB) ...
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bioRxiv - Microbiology 2023Quote: ... a KOZAK sequence was added in front of the TY1 region with the Q5 Site-Directed Mutagenesis Kit (NEB) using primers F167 and F168 ...
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bioRxiv - Genomics 2023Quote: 500 nanograms of total RNA were used for NEBNext® Ultra II Directional RNA Library Prep Kit (NEB # E7760L). Poly-A selection (NEBNext Poly (A ...
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bioRxiv - Genomics 2023Quote: ... The remaining thirteen samples had paired-end sequencing libraries constructed according to the manufacturer’s specifications for the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB). Three samples (RMF031 ...
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bioRxiv - Molecular Biology 2023Quote: ... pcDNA3.1-ATP6V1H(Δ176-191)-FLAG and pcDNA3.1-ATP6V1H(Δ176-193)-FLAG were generated using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs). pENTR-ATP6V1H-FLAG was produced using the pENTR/D-TOPO Cloning Kit (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... Site-specific mutagenesis of efp was carried out with the Q5® Site-Directed Mutagenesis Kit (New England BioLabs) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... Replacement of the interchangeable sgRNA was accomplished using a Q5 site-directed mutagenesis kit (Table S3) (New England BioLabs). The TgLaforin-HA tagging construct was generated by amplifying the 3’ end of the TgLaforin-HA construct generated for complementation (see generation of COMP line below and Tables S2 and S3 ...
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bioRxiv - Molecular Biology 2023Quote: Sequencing libraries were prepared using the NEBNext Ultra II DNA library prep kit according to the manufacturer protocol (NEB). Sequencing libraries were pooled and analyzed on a NextSeq 550 platform using paired-end mode with 32 cycles on both ends ...
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PRC1 directs PRC2-H3K27me3 deposition to shield adult spermatogonial stem cells from differentiationbioRxiv - Developmental Biology 2023Quote: ... Libraries were constructed using NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® (NEB, E6420S) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries were prepared using the NEB Ultra II Directional RNA Library Prep Kit (New England BioLabs, Ipswich, United States), and all samples were run on one lane of the NovaSeq6000 S4 v1.5 (Illumina ...
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bioRxiv - Developmental Biology 2023Quote: ... About 1ng DNA was used for library construction using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB). Sequencing (2× 150bp ...
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bioRxiv - Bioengineering 2023Quote: ... 20 ng of each integrase amplicon was used per reaction using PURExpress® In Vitro Protein Synthesis Kit (NEB) in a total volume of 9 µl ...
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bioRxiv - Neuroscience 2023Quote: ... deposited by Bischof and Basler) at the EcoRI restriction site by NEBuilder HiFi DNA Assembly Cloning Kit (NEB #E5520S). The pUASTattB-Gs2 vector was injected to y1 w67c23 ...
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bioRxiv - Neuroscience 2023Quote: ... Sequencing libraries were generated using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2023Quote: Mutant variants of the mS29-containing construct were generated using the Q5 site-directed mutagenesis kit (New England Biolabs). Mutagenesis primers were designed using the NEBaseChanger tool ...
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bioRxiv - Evolutionary Biology 2023Quote: ... using the Monarch® HMW DNA Extraction Kit for Tissue (Cat. No. T3060S, New England BioLabs Inc., MA, USA). DNA from resequencing samples was isolated using either a modified protocol for the DNeasy Blood and Tissue Kit (Cat ...
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bioRxiv - Genetics 2023Quote: ... which was immediately used as a template for seven synonymous single-nucleotide substitutions into the terminal 21bp of the ZK938.12 gene fragment by Q5 site-directed mutagenesis kit (NEB). The resultant plasmid was named pDSP47.
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription of RNA and quantitative PCR was performed using the Luna Universal One-step RT-qPCR Kit (NEB) using 1 µg of RNA per reaction ...
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bioRxiv - Molecular Biology 2023Quote: ... The gel purified DNA fragments were cloned into the pMiniT 2.0 vector using the NEB PCR Cloning Kit (New England BioLabs) and then transformed into NEB 10-beta competent cells (New England BioLabs) ...
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bioRxiv - Microbiology 2023Quote: ... and directional cDNA libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB). cDNA library quality was assessed using a High Sensitivity DNA Kit (Agilent) ...
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bioRxiv - Molecular Biology 2023Quote: ... T7 transcription was performed for 16 h and then RNA was purified using the Monarch RNA Cleanup Kit (NEB). In vitro cleavage was performed with purified recombinant ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by strand-specific cDNA NGS library preparation (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina, NEB). The size of the resulting library was controlled by use of a D1000 ScreenTape (Agilent 2200 TapeStation ...
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bioRxiv - Microbiology 2023Quote: 20 ng of nucleic acid was enriched for viral genomic sequences using NEBNext Microbiome DNA Enrichment Kit (NEB, E2612) to reduce host genomic DNA contamination ...
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bioRxiv - Plant Biology 2023Quote: ... and each individual library was barcoded using the NEBNext® Multiplex Oligos for Illumina® kit (New England Biolabs).
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bioRxiv - Cancer Biology 2023Quote: ... and PCR enrichment were performed sequentially using the NEBNext Ultra™ II DNA Library Prep Kit for Illumina (NEB). Lastly ...
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bioRxiv - Synthetic Biology 2024Quote: ... then inserted the library cassette into the digested pMPRA1 backbone using the NEBuilder HiFi DNA Assembly kit (NEB E2621). This intermediate plasmid was purified with Kapa Pure Beads (Roche KK8002) ...
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bioRxiv - Genetics 2024Quote: ... Libraries were constructed with NEBNext® Ultra™ II FS DNA Library Prep Kit (New England Biolabs, Ipswich, MA).
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bioRxiv - Cell Biology 2024Quote: ... The pooled library was once again bead purified and quantified by qPCR using NEBNext Library Quant Kit (NEB, E7630). The library size was verified using a High Sensitivity DNA Kit on a 2100AB Bioanalyzer instrument (Agilent) ...
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bioRxiv - Developmental Biology 2024Quote: ... The RNA-sequencing (RNA-seq) libraries were generated using the NEBNext Ultra Directional RNA Library Prep Kit (NEB, E7420L), The yield of cDNA libraries was measured using the Qubit dsDNA HS Assay Kit ...
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bioRxiv - Immunology 2024Quote: ... Total RNA samples (5-500 ng) were hybridized with NEBNext rRNA Depletion Kit v2 (Cat# E7400; New England Biolabs) to diminish rRNA from the samples ...
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bioRxiv - Molecular Biology 2024Quote: ... Genomic DNAs (gDNAs) were extracted using the Monarch HMW DNA extraction kit for Tissue from New England Biolabs (NEB) with the following modifications ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were prepared using the NEBNext Ultra™ II Directional RNA Library Prep Kit for Illumina (New England Biolabs). sPOINT was performed in the same manner with the following difference ...
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bioRxiv - Biochemistry 2024Quote: ... a vector containing an N-terminal Thioredoxin-His10 tag using a Gibson Assembly® Cloning Kit (New England BioLabs). Plasmids were extracted and transformed into competent E ...