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Citations for New England Biolabs :
5651 - 5700 of 9547 citations for QuantiChrom Phosphate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: Illumina libraries were prepared using the NEBNext Ultra™ II DNA Library Prep Kit (New England Biolabs, Frankfurt, Germany) following the instructions of the manufacturer ...
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bioRxiv - Cell Biology 2021Quote: sgRNAs were generated by in vitro transcription using the Hiscribe T7 high yield RNA synthesis kit (New England Biolabs). Purified sgRNA (0.5 μg ...
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bioRxiv - Evolutionary Biology 2021Quote: ... see Supplementary Data 6) and cloned into pDHFR vector provided in the PURExpress In Vitro Protein Synthesis Kit (NEB). The translation into liposomes was done as described previously95 and the output was analyzed by Blue Native PAGE using 2% digitonin and NativePAGE Novex 4-16% Bis-Tris Protein Gel (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... The RNA-seq libraries were constructed by NEBNext Ultra II RNA Library Prep kit (NEB, Cat: E7775, E7335, E7500) and sequenced by Illumina NextSeq 500 system (Duan et al. ...
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bioRxiv - Genomics 2021Quote: ... Libraries were constructed using the NEBNext Ultra II DNA Library Preparation Kit (New England Biolabs, Frankfurt am Main, Germany) following the manufacturer’s description ...
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Revisiting the role of Toxoplasma gondii ERK7 in the maintenance and stability of the apical complexbioRxiv - Microbiology 2021Quote: ... ERK7-mAID-HA (TGGT1_233010) was obtained by transfecting a gRNA obtained by Q5 site-directed mutagenesis kit (New England Biolabs) on the pSAG1::Cas9-U6::sgUPRT vector (Shen et al ...
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bioRxiv - Immunology 2020Quote: ... Sequencing libraries were generated from sonicated DNA with the NEBNext Ultra II DNA Library Prep Kit (New England BioLabs). Ligation products were purified with SPRIselect beads (Beckman Coulter ...
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bioRxiv - Cell Biology 2020Quote: ... R972A mutations were introduced into the aforementioned IRS2 clones using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs) with the primers 5’ - AGA TTA TAT GAA TAA GTC CAC TGT CAG ATT ATA TG - 3’ and 5’ - GAC AGT GGA CTT GCC TGG CGA GAG TCT GAA CT - 3’ according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2019Quote: 5’-Phosphorylated linker (Oligonucleotide K, Supplementary Table 6) was adenylated using a 5’ DNA Adenylation Kit (New England Biolabs) at 20x scale and purified by TRIzol extraction as previously described56 ...
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bioRxiv - Microbiology 2019Quote: ... The pJ107106 promoter was converted to pJ107111 (Zucca et al., 2015) using the Q5 site-directed mutagenesis kit (NEB), with suggested protocols ...
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bioRxiv - Genomics 2021Quote: ... High-molecular weight DNA was extracted from blood with a Bionano Plant Tissue DNA isolation kit (Bionano Genomics) and digested with Nt.BspQI nickase (New England Biolabs). After labeling and staining ...
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bioRxiv - Genomics 2021Quote: ... and NEBNext® ultra™ II directional RNA library prep kit for Illumina (NEB #E7760, New England Biolabs, Inc.). Paired-end sequencing (75 bp ...
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bioRxiv - Genomics 2021Quote: ... and NEBNext® ultra™ II directional RNA library prep kit for Illumina (NEB #E7760, New England Biolabs, Inc.). Paired-end sequencing (75 bp ...
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bioRxiv - Genetics 2020Quote: ... TruSeq-barcoded RNA-seq libraries were generated with the NEBNext Ultra II RNA Library Prep Kit (New England Biolabs) and each library was quantified via Qubit 2.0 (dsDNA HS kit ...
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bioRxiv - Cell Biology 2020Quote: ... The libraries were constructed by using the NEBNext ultra II RNA library prep kit (New England BioLabs, Ltd., USA) and the qualities were analyzed with Agilent 2100 Bioanalyzer (Agilent ...
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bioRxiv - Genomics 2021Quote: ... and enrichment using the NEBNext® Ultra™II DNA Library Preparation kit (New England Biolabs Inc, Ipswich, MA). Titration of the number of PCR cycles was performed as described in Belton et al ...
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bioRxiv - Genomics 2021Quote: ... TruSeq-barcoded RNAseq libraries were generated with the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs). Each library was quantified with a Qubit 2.0 (dsDNA HS kit ...
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bioRxiv - Genomics 2021Quote: ... Illumina sequencing libraries were prepared using NEBNext rRNA Depletion and Ultra Directional RNA library prep kits (New England Biolabs) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: A library was prepared using New England Biolabs Ultra II DNA Kit for Illumina (New England Biolabs, United States) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We constructed the cDNA library using NEBNext®Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Bulk RNA sequencing libraries were constructed using the Nebnext Ultra II Directional RNA Library Prep Kit (New England Biolabs) and sequenced on a Nextseq 500 or Novaseq 6000 device ...
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bioRxiv - Biochemistry 2021Quote: ... 70 and 80 amino acids deletion mutants were carried out with the Q5 mutagenesis kit (New England Biolabs, MA). All AtAtm3 constructs were overexpressed in Escherichia coli BL21-gold (DE3 ...
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bioRxiv - Biochemistry 2021Quote: ... Both fragments were run on a 1% agarose gel and purified using a Monarch DNA Gel Extraction kit (NEB). The fragments were then joined using the NEBuilder HiFi DNA Assembly protocol (NEB).
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bioRxiv - Plant Biology 2021Quote: ... The sequences were used to create libraries with NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs).
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bioRxiv - Developmental Biology 2021Quote: ... The RNA libraries were constructed using NEBNext Ultra Directional RNA Library Prep Kit for Illumina following manufacturer's recommendations and Oligo (dT) beads (NEB) were used to isolate poly (A ...
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bioRxiv - Immunology 2021Quote: ... The sequencing library was prepared using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®(NEB) and xGen™ UDI-UMI Adapters (IDT ...
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bioRxiv - Biochemistry 2021Quote: ... c-Jun point mutations were generated by PCR-based mutagenesis using the site-directed mutagenesis kit (New England Biolabs). cDock ...
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bioRxiv - Microbiology 2022Quote: ... and selection marker of pSIN was replaced to match that of pMCB306 using the Gibson Assembly Cloning Kit (NEB) to generate the transfer plasmid (BAS2186 ...
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bioRxiv - Microbiology 2021Quote: Metagenomic shotgun libraries were prepared using New England BioLabs Ultra FS II library kit (New England BioLabs, Ipswich, MA) and cleaned with a 1:1 ratio of Sera-Mag speedbeads after ligation (Rohland and Reich ...
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bioRxiv - Biochemistry 2020Quote: ... Y85F and Y144F mutants of His6-MmfR were created using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Libraries were prepared for sequencing using the NEBNext Ultra II and Multiplex Oligios for Illumina kits (New England Biolabs) according to the manufacturers protocol ...
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bioRxiv - Cell Biology 2019Quote: The CMV-EPO-eGFP-p2A-A1AT-mCherry construct was assembled using the NEBuilder HiFi DNA assembly cloning kit (NEB) using vector sequences derived from PCSK9-eGFP-p2A-A1AT-mCherry (32 ...
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bioRxiv - Genetics 2021Quote: ... The latter was prepared using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB, Ipswitch, MA, USA). Briefly ...
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bioRxiv - Bioengineering 2021Quote: ... Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (#E7530L, NEB, USA) following the manufacturer’s recommendations and index codes were added ...
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bioRxiv - Biophysics 2021Quote: ... The in vitro mRNA synthesis was done using the HiScribe T7 ARCA mRNA Kit (New England Biolabs, Ipswich, MA) or the mMESSAGE T7 ULTRA Transcription Kit (ThermoFisher ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We prepared DNA whole genome sequencing libraries using NEBNext UltraTM II DNA Library Prep Kit (Cat. E7645L, NEB Inc.). We quantified DNA on QubitTM 3.0 fluorometer using Qubit High sensitivity dsDNA Assay (#Q32854 ...
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bioRxiv - Cancer Biology 2021Quote: ... DNA libraries for Illumina sequencing were prepared with the NEBNext Ultra 2 DNA Library Kit for Illumina (NEB, E7645L) using 200ng of DNA and custom made unique dual indices (8bp) ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 μg of total RNA was used in cDNA synthesis using ProtoScript II Reverse Transcription kit (NEB, Cat # e6560) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 1995) by Gibson assembly using NEBuilder HiFi DNA Assembly Cloning Kit according to the manifacturer’s protocol (New England Biolabs). The fragments for the assembly were prepared by PCR using Q5 High Fidelity polymerase (New England Biolabs) ...
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bioRxiv - Cell Biology 2020Quote: Epsin mutant constructs were generated successively by mutagenesis using Q5® Site-Directed Mutagenesis Kit (New England Biolabs Inc.). Clathrin binding domain 1 (CBD1 ...
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bioRxiv - Biochemistry 2021Quote: ... with a single-cysteine at position 336 was created from the dimeric cysteine-free MutS variant (24,30,31) using the NEBuilder® HiFi Assembly Cloning Kit (NEB) (see Supplementary Data for details ...
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bioRxiv - Bioengineering 2021Quote: For the construction of pUC18_Apdp4 (Fig. S18) a gene assembly strategy using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs) was followed ...
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bioRxiv - Genomics 2020Quote: ... The donor vector (mCTCF24-AID-donor-Neo) was constructed using PCR and Gibson Assembly Cloning kit (New England Biolabs). The insert cassette includes sequences that codes for a 5GA linker ...
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bioRxiv - Genomics 2019Quote: ... 12 cycles of PCR were performed as indicated by the NEBNext multiplex oligos for Illumina kit (New England Biolabs). PCR reactions were cleaned using Agencourt AMPure XP beads (Beckman).
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bioRxiv - Genomics 2019Quote: ... and then used for 350-bp paired-end library construction with the Next Ultra DNA library prep kit (NEB) for Illumina sequencing ...
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bioRxiv - Molecular Biology 2021Quote: ... ExRai AMPKAR T/A was generated via Gibson Assembly using NEBuilder HiFi DNA Assembly Kit (New England Biolabs E2621) using primers 1-2 ...
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bioRxiv - Molecular Biology 2021Quote: ... libraries were made using the NEBNext Ultra II Directional RNA Library Prep Kit following the polyA workflow (NEB, E7760). A custom protocol was used at Novogene for libraries preparation ...
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bioRxiv - Molecular Biology 2021Quote: ... the newly transcribed reporter mRNA was isolated using the Monarch RNA Cleanup Kit (New England Biolabs, Cat. No. T2040L), eluted with 1 mM sodium citrate pH 6.4 (Gene Link ...
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bioRxiv - Microbiology 2020Quote: ... RdRP_SARSr-F2 (GTGARATGGTCATGTGTGGCGG) and dRP_SARSr-R1(CARATGTTAAASACACTATTAGCATA) using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) and a CFX96 Real-Time System ...
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bioRxiv - Microbiology 2020Quote: ... cDNA libraries were prepared with the NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England Biolabs) following manufacturer’s recommendations ...