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Citations for New England Biolabs :
501 - 550 of 2416 citations for Rat SEPT12 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... These plasmids were then double digested by SalI and NotI (NEB), and the fragment containing CMV promoter and coding sequence were purified and 0.5 nl of a 200ng/μl ...
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bioRxiv - Microbiology 2020Quote: ... pMQ30MRR1 complementation plasmids was digested with Not1-HF (New England BioLabs) for transformation.
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bioRxiv - Microbiology 2020Quote: ... MRR1 complementation plasmids were linearized with Not1-HF (New England BioLabs), cleaned up the Zymo DNA Clean & Concentrator kit (Zymo Research ...
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bioRxiv - Developmental Biology 2022Quote: The pEntr-EMS plasmid was generated from a HiFi reaction (NEB) using the pEntr3c Gateway cloning (Invitrogen ...
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bioRxiv - Physiology 2022Quote: ... The pCMV-myc plasmid was digested with EcoRI (New England Biolabs). The PCR products and digested plasmids were electrophoresed ...
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bioRxiv - Microbiology 2022Quote: ... Plasmid transformation was carried out according to the manufacturer’s protocol (NEB). The purification of PT-dependent restriction enzyme EcoWI was performed as previously described (21).
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bioRxiv - Cancer Biology 2022Quote: ... the plasmid was linearized with BstEII-HF (NEB, Cat. No. R3162) and MluI-HF (NEB ...
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bioRxiv - Microbiology 2019Quote: ... and ligated into similarly digested plasmids using T4 DNA ligase (NEB). Ligation reactions were transformed into chemically competent TOP10 E ...
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bioRxiv - Biochemistry 2019Quote: ... The plasmid pNZ-SV was amplified by Phusion DNA polymerase (NEB) with the primer pair infupNZ-SVfor and infupNZ-SVrev (Supplementary File 4 ...
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bioRxiv - Biochemistry 2019Quote: ... The plasmid pNZ-SV10H was amplified by Phusion DNA polymerase (NEB) with the primer pair linpNZ-SVfor and linpNZ-SVrev (Supplementary File 4 ...
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bioRxiv - Biochemistry 2019Quote: ... The plasmid pIL3BTC was digested with the restriction enzymes NotI (NEB) and BstXI (NEB ...
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bioRxiv - Microbiology 2019Quote: Plasmids were constructed using the Gibson Assembly protocol (New England Biolabs). Primers were designed with http://nebuilder.neb.com ...
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bioRxiv - Genomics 2019Quote: ... We then ligated the plasmid and inserts using T4 ligase (NEB) in a 1:3 ratio respectively ...
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bioRxiv - Molecular Biology 2019Quote: ... The purified plasmid was subsequently cut using either EcoRI-HF (NEB) or SapI-HF (NEB ...
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bioRxiv - Microbiology 2019Quote: ... Plasmids were purified and analyzed by BstEII digestion (New England Biolabs) for confirmation of correct construction and electroporated into electrocompetent E ...
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bioRxiv - Genetics 2019Quote: ... after the recombinant plasmids linearized with NotI Restriction Enzyme (NEB, USA), and then the capped mRNAs were purified by RNeasy Mini Kit (Qiagen ...
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bioRxiv - Cell Biology 2020Quote: ... these plasmids were digested with BamHI and NotI (New England Biolabs) and subcloned into BamHI/NotI-digested pACT (Ohkawa et al. ...
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bioRxiv - Cell Biology 2020Quote: ... This plasmid was digested with MluI (New England Biolabs, Ipswich, MA), removing 74% of the arif-1 coding region ...
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bioRxiv - Synthetic Biology 2020Quote: ... The Monarch Plasmid Miniprep Kit (New England Biolabs, Ipswich, MA, USA) was used to isolate plasmids ...
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bioRxiv - Molecular Biology 2021Quote: ... The pNLrep plasmid was digested with KpnI and PsiI enzymes (NEB) giving a 6337 bp product ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid nicking with Nb.BssSI was carried out via manufacturer’s recommendations (NEB). Both reactions were incubated at 37 °C for 2 hours ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids were constructed using Gibson Assembly (New England Biolabs, Ipswich, MA). Regions of plasmids constructed using PCR were verified by DNA sequencing ...
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bioRxiv - Synthetic Biology 2020Quote: The pLib_sfGFP plasmid was first digested with SalI-HF (NEB#R3138S) and NheI (NEB# R3131S ...
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bioRxiv - Synthetic Biology 2019Quote: Plasmids were assembled using Gibson assembly (New England Biolabs, Cat#E2611S) and purified using a Qiagen QIAfilter Midiprep Kit (QIAGEN ...
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bioRxiv - Biochemistry 2021Quote: ... Plasmid pETconV4 was linearized by digestion with NdeI and XhoI (NEB). Both fragments were run on a 1% agarose gel and purified using a Monarch DNA Gel Extraction kit (NEB) ...
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bioRxiv - Evolutionary Biology 2021Quote: Repair template plasmids were assembled using the NEBuilder HiFI Kit (NEB) from a combination of restriction digest fragments and PCR products ...
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bioRxiv - Developmental Biology 2021Quote: ... Plasmid fingerprinting was undertaken using Bbs1 and EcoRV restriction enzymes (NEB) to confirm the EnVT15159 product had been inserted into the plasmid in the correct orientation ...
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bioRxiv - Developmental Biology 2021Quote: ... linearized plasmids using Not1 and Sac1 restriction enzymes (New England Biolabs), and synthesized probes using a reverse transcription kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: The plasmid pYES2/CT was firstly digested by EcoRI/XbaI (NEB) restriction enzymes ...
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bioRxiv - Synthetic Biology 2021Quote: ... eGFP plasmids were digested with BamHI (New England BioLabs Inc., R0136L) at 37°C for 1 hour and agarose gel-purified with GenCatch Advanced Gel Extraction Kit (Epoch Life Science ...
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bioRxiv - Developmental Biology 2020Quote: ... pCS2-CDHR1a and CDHR1a LMA plasmids were linearized with NotI (NEB). mRNA was synthesized using SP6 mMessage mMachine kit (Ambion ...
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bioRxiv - Biochemistry 2021Quote: ... plasmid DNA was incubated with the ScaI-HF (New England Biolabs) for 3196 bp Phagemid GATC1 or EcoRI (New England Biolabs ...
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bioRxiv - Genetics 2020Quote: ... plasmid p07544_OEC carrying gul1 was used for Q5 mutagenesis (NEB biolabs). Using specific primers (S5 Table) ...
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bioRxiv - Genomics 2021Quote: ... the plasmids were digested with the restriction endonuclease BsaI (NEB Biolabs) to isolate the transformation cassette of interest ...
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bioRxiv - Genomics 2021Quote: ... the plasmids were digested with the restriction endonuclease BsaI (NEB Biolabs) to isolate the transformation cassette of interest ...
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bioRxiv - Microbiology 2021Quote: ... The mini-CTX-lacZ plasmid was linearized by HindIII (NEB, USA) at 37°C for 4 hrs ...
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bioRxiv - Microbiology 2020Quote: ... Linearized plasmids were re-ligated using T4 DNA ligase (NEB M0202). Fragments were amplified using Q5 DNA polymerase (NEB 0491 ...
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bioRxiv - Immunology 2021Quote: ... Plasmid vectors were linearized using XbaI (New England Biolabs, Cat R0145S) and purified using QIAquick PCR purification kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: PCR amplicons and plasmids were purified using Monarch DNA kits (NEB). PCR ...
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bioRxiv - Microbiology 2022Quote: ... The plasmid was religated with T4 DNA ligase (New England Biolabs) and transformed into TOP10 E ...
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bioRxiv - Cell Biology 2022Quote: ... The assembled plasmid library was electroporated into electrocompetent bacteria (NEB, C3020K) according to the manufacturer’s instructions as 3 reactions ...
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bioRxiv - Developmental Biology 2022Quote: ... linearized plasmids using Not1 and Sac1 restriction enzymes (New England Biolabs), and synthesized probes using a reverse transcription kit (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids (S2 Table) were constructed using HiFi assembly (New England Biolabs) or BEVA modular golden-gate assembly (68 ...
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bioRxiv - Microbiology 2022Quote: ... Plasmid-backbone PCRs were restriction digested with DpnI (New England Biolabs) following the manufacturers’ protocol ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids and inserts were assembled using NEBuilder HiFi DNA Assembly (NEB), followed by incubation at 50 °C for 20 min ...
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bioRxiv - Microbiology 2022Quote: ... PCR products and plasmids were purified with Monarch DNA kits (NEB). Overlapping primers for amplification were designed using the NEBuilder assembly tool (https://nebuilderv1.neb.com/ ...
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bioRxiv - Microbiology 2022Quote: ... all plasmids were assembled through NEBuilder® HiFi DNA Assembly (NEB). The basic backbone plasmid pCOMA_NT-crRNA was constructed by amplifying the pCB102 ori ...
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bioRxiv - Microbiology 2022Quote: ... The plasmid pEKE2 was linearized with SacI (New England Biolabs, U.S.A.) followed by assembling of the amplified gene via Gibson Assembly according to the manufacturer’s instructions (Gibson Assembly® Master Mix ...
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bioRxiv - Synthetic Biology 2022Quote: ... and sRNA plasmids using BsaI Type IIS Enzyme (New England Biolabs) and transformed into E ...
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bioRxiv - Developmental Biology 2024Quote: ... pXPR_050 plasmids were digested using the Bsmb1 restriction enzyme (NEB; R0580). The synthesized double-strand oligo pools and digested pXPR_050 plasmids were ligated using T7 ligase (NEB ...