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Citations for New England Biolabs :
501 - 550 of 976 citations for Dl 4 Methoxyestradiol 13 14 15 16 17 18 13C6 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... and resolved on a 4–20% SDS-polyacrylamide gel (NEB). The presence of MeCP2 was assayed by western blotting using anti-MeCP2 monoclonal antibody M6818 (Sigma ...
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bioRxiv - Genetics 2023Quote: ... column-purified and transformed in 4 or 5 electroporations (NEB 10-beta Electrocompetent E ...
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bioRxiv - Systems Biology 2023Quote: ... and SpeI-HF (New England Biolabs, Ipswitch, MA;, and 4) the barcode fragment digested with BstEII-HF (New England Biolabs ...
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bioRxiv - Neuroscience 2023Quote: ... 4°C) and filled in using T4 DNA polymerase (NEB) using manufacturers’ instructions at 12°C for 20 min75 ...
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bioRxiv - Biophysics 2023Quote: ... “no SDS” loading dye (New England Biolabs, UK; 4 µL) was added to each sample (15 µL ...
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bioRxiv - Immunology 2023Quote: ... and 4 by using the high-fidelity Phusion (NEB Biolabs) 3-step PCR protocol ...
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bioRxiv - Immunology 2023Quote: ... and 4 by using the high-fidelity Phusion (NEB Biolabs) 3-step PCR protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... 4 µl of thermolabile proteinase K (New England Biolabs, P811S) added to Mitochondria-bound beads resuspended in 30 µl of KPBS ...
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bioRxiv - Genetics 2021Quote: The purified DNA was PCR amplified for 15 cycles using Q5 High Fidelity DNA polymerase (New England Biolabs, M0491S) with the incorporation of Illumina Nextera XT adaptors (Illumina ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR amplification was run for 15 cycles and NEBNext Index1-24 primers for Illumina were used (New England Biolabs).
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bioRxiv - Developmental Biology 2019Quote: ... libraries were generated using the NEBNext Ultra II library preparation kit with 15 cycles of PCR amplification (NEB E7645). The libraries for the ‘MicroChIP’ samples were generated using the MicroPlex library prep kit (Diagenode C05010012 ...
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bioRxiv - Genomics 2019Quote: ... Adapter ligation (BAM adapters) was performed for 15 minutes using NEBNext Quick Ligation Module (New England Biolabs, MA, USA). Library mix was cleaned up using 0.4X AMPure XP beads (Beckmann Coulter ...
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bioRxiv - Developmental Biology 2021Quote: ... MgCl2 was added to a final concentration of 50mM and 12.2µl of each tagmented sample was PCR amplified for 15 cycles using 2x NEB Next HiFi PCR mix (NEB). Amplified libraries were purified using the Qiagen PCR purification MinElute kit.
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bioRxiv - Microbiology 2020Quote: ... 15 μg of DNA-depleted RNA was incubated with 25 units of calf intestinal alkaline phosphatase (CIP) (#M0290, NEB) in a 50 μl reaction volume for 1 h at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... The pellets containing intact nuclei were vortexed at maximum setting for 15 sec with the nuclear extraction buffer (NEB) and then incubated at 4°C for 30 min with gentle mixing ...
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bioRxiv - Molecular Biology 2022Quote: ... Up to 5 μg of gDNA was digested with 15 U of SalI-HF® enzyme (NEB, Cat# R3138L) for 3 hr at 37°C and separated on 0.9% agarose gel for 16 hr at 50 V ...
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bioRxiv - Biochemistry 2022Quote: ... The N-linked glycans and the CBP tag were cleaved by endoglycosidase H (Endo H, ∼0.2 mg per 10–15 mg purified protein, New England Biolabs) and HRV-3C protease (∼2 mg per 10–15 mg purified proteins ...
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bioRxiv - Molecular Biology 2019Quote: ... The samples were then transferred on to the ice and the components (15 μl Blunt ligase master mix, 2.5 μl NEB Next adaptor for Illumina ...
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bioRxiv - Molecular Biology 2019Quote: ... followed by 10 µl of 10X NEB2 buffer and 15 µl of HindIII (New England Biolabs, 20 U/µl) to digest at 37°C for 2 hr ...
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bioRxiv - Cancer Biology 2020Quote: ... Lysates were centrifuged for 15 min at 30,700 x g and the collected supernatants were incubated with amylose resin (New England Biolabs) for 1 h at 4°C ...
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bioRxiv - Genetics 2020Quote: ... Fragments were either PCR amplified with a least 15 bp overlap at each end (Phusion Hot Start Flex NEB M0535L ...
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bioRxiv - Genetics 2019Quote: ... followed by 10 μl of 10X NEB2 buffer and 15 μl of HindIII (New England Biolabs, 20 U/μl) to digest at 37°C for 2 hr ...
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bioRxiv - Systems Biology 2021Quote: ... 3 μL of the reverse phasing primer pool (100 nM) and 15 μL of Q5 Mastermix (New England Biolabs). Cycle conditions were 4 minutes at 98°C followed by 20x (30 seconds at 98°C ...
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bioRxiv - Genomics 2020Quote: ... followed by addition of 20 μl Ligation master mix (containing: 15 μl NEB Quick ligation buffer, 0.4 μl NEB Quick Ligase ...
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bioRxiv - Genomics 2020Quote: ... library DNA was recovered using AMPure XP beads (1.8X concentration) and PCR amplified for 15 cycles (all enzymes from New England Biolabs). Libraries were sequenced on an Illumina NextSeq in 37×37 paired end mode ...
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bioRxiv - Zoology 2023Quote: ... We performed 5 PCR enrichment with 15 cycles (30 ng DNA input, NEB Phusion High-Fidelity PCR Master Mix) for each library to increase fragment diversity ...
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bioRxiv - Microbiology 2023Quote: ... The 30 μl PCR reaction system contained 15 μl of Phusion high-fidelity PCR Master Mix (New England Biolabs), 0.2 μM forward and reverse primers ...
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bioRxiv - Cell Biology 2023Quote: ... The newly generated DSBs by the sonication step were also processed with T4 DNA polymerase (15 U) (NEB; M0203), Klenow fragment (5 U ...
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bioRxiv - Molecular Biology 2023Quote: ... samples were washed in CutSmart buffer, and incubated (1h, RT) in 150 µL blunting mix (112.5 µL ddH2O, 15 µL 10x blunting buffer, NEB, 15 µL 100 µM dNTPs ...
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bioRxiv - Genomics 2023Quote: ... All PCR reactions were performed with 15 μL of Phusion® High-Fidelity PCR Master Mix (New England Biolabs), 0.2 μM of forward and reverse primers ...
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bioRxiv - Cell Biology 2020Quote: ... Annealed handles were mixed with the purified 21 kb ARS1-DNA at a molar ratio of 15:1 and ligated with T4 DNA Ligase in 1 × T4 ligase buffer (both NEB) at 16 °C overnight ...
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bioRxiv - Genomics 2020Quote: ... A 20 μL aliquot of DNA was repaired (15 min, 25 °C) in a 40 μL reaction using T4 DNA polymerase (New England Biolabs). After purifying the DNA (MinElute™ Reaction Cleanup Kit ...
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bioRxiv - Molecular Biology 2021Quote: ... Genomic DNA was isolated with Proteinase K lysis buffer and 15 µg of DNA were digested overnight with 40 units of MspI (New England Biolabs). Digested DNA was separated on a 0.8% agarose gel ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 mM EDTA) at 65°C for 15 minutes and later the proteins were digested by 10U Proteinase K (NEB) at 37°C for 30 minutes ...
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bioRxiv - Microbiology 2019Quote: ... DpnI treatment was performed for 15 min at 37°C and the four different amplified parts were purified and then digested with BsmBI (NEB) enzyme at 55 C for 1.5 hrs ...
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bioRxiv - Developmental Biology 2020Quote: DNA was extracted from embryonic membranes or tail tissue using the HotShot method for 15 minutes (Truett et al., 2000) and 2 µl DNA was used for PCR with Phusion polymerase (New England Biolabs). For Geminin and Cre PCRs ...
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bioRxiv - Biochemistry 2019Quote: ... The pellet was resuspended in 28 µl hypertonic buffer and incubated at room temperature for 15 min with 1000 gel units of micrococcal nuclease (NEB). Chromatin-bound proteins were released from the DNA by addition of 7 µl of 1 M ammonium sulfate on ice for 5 minutes ...
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bioRxiv - Microbiology 2021Quote: ... 15 μL of PCR product were mixed with 5 μL of a 5X Exo-SAP solution (15% Shrimp Alkaline Phosphatase – 1000U/ ml – NEB, 10% Exonuclease I – 20000 U/ ml – NEB ...
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bioRxiv - Biophysics 2020Quote: For the insertion of an ATTO647N-labeled oligonucleotide complementary to the position 14711 bp from the biotinylated end of the λ DNA we employed the previously described strategy14 and followed the more recently described procedure.15 2 μg of λ DNA was incubated for 2 hours with the nicking enzyme Nt.BstNBI (20 units, NEB) at 50 °C in the nickase buffer ...
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bioRxiv - Biochemistry 2021Quote: ... contains sequences from −100 to +15 of the bhsA promoter fused to the tR’ terminator inserted between the HindIII and SalI sites of pACYC184 (NEB). Mutant derivatives of pBEN516 containing a G:C base pair at position TSS-2 (pKS494 ...
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bioRxiv - Biochemistry 2020Quote: ... The insert and vector DNA were combined in a 3:1 insert:vector molar ratio in a volume of 5 μL and added 15 μL of Gibson Assembly Master Mix (New England BioLabs), consisting of T5 Exonuclease ...
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bioRxiv - Cell Biology 2021Quote: ... the protein mix was incubated for 1 h at 4° C in 150 µL pull-down buffer supplemented with 15 µL glutathione agarose resin for GST pull-downs or amylose resin (New England Biolabs) for MBP pull-downs ...
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bioRxiv - Microbiology 2021Quote: ... Polymerase chain reactions were performed with 15 μL Phusion® High-Fidelity PCR Master Mix (New England Biolabs, Ipswich, USA), 0.2 μM of forward primer ...
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bioRxiv - Microbiology 2020Quote: ... Samples were boiled for 15 minutes and either cooled on ice (no proteinase K) or incubated with proteinase K (NEB) at 55°C for 1 hour ...
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bioRxiv - Molecular Biology 2022Quote: ... the sRNA fraction (∼15- 35nt) was isolated by gel extraction and RNA species bearing 5’-OH were monophosphorylated by T4 polynucletide kinase (NEB). This was followed by treatment by a terminator exonuclease (Epicentre) ...
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bioRxiv - Genomics 2022Quote: TopoI concentration required to relax 50% of pUC18 topoisomers was determined after 15 min of incubation at 37°C in rCutSmart Buffer (NEB). For the inhibition assays ...
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bioRxiv - Molecular Biology 2022Quote: ... 200 fmol of input cDNA were incubated for 20 minutes at room temperature (15–25°C) with the native barcodes and Blunt/TA Ligase Master Mix (New England Biolabs), followed by 10 minutes at 65°C.
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bioRxiv - Neuroscience 2020Quote: ... libraries were prepared in an additional 15 amplification cycles using the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs) and sequenced on the HiSeq2000 platform (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... All PCR reactions were carried out with 15 μL of Phusion® High-Fidelity PCR Master Mix (New England Biolabs); 0.2 μM each of forward and reverse primers ...
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bioRxiv - Molecular Biology 2021Quote: ... 95°C/15 s and annealing/extension: 65°C/5 min) of PCR using Q5 high-fidelity DNA polymerase (NEB). Amplicons were purified using 1× AMPure XP beads (Beckman Coulter) ...