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Citations for New England Biolabs :
5201 - 5250 of 10000+ citations for Rat Insulin Like Growth Factor 1 IGF1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: sgRNAs were then transcribed from templates using HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) and purified using Megaclear Kit (Life Technologies) ...
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bioRxiv - Microbiology 2019Quote: ... The gltA complement plasmid was constructed using a Gibson Assembly Cloning Kit (New England Biolabs, Ipswich, MA). Briefly ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and whole genome sequencing libraries were prepared using the NEBNext Ultra II Library kit (New England Biolabs) according the recommended protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Transcriptomic sequencing of RNA was performed using NEBNext® rRNA Depletion Kit (New England Biolabs, Inc.; E6310S) and SMARTer Stranded Total RNA-Seq Kit - Pico Input (Clontech ...
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bioRxiv - Bioengineering 2019Quote: ... The NsiI site was then removed using the Q5 Site-directed mutagenesis Kit (New England Biolabs, E0554S). All sgRNA sequences for activation studies (Supplementary Table 1a ...
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bioRxiv - Neuroscience 2019Quote: ... and 24 hours of incubation GLuc activity was quantified using the BioLux Gaussia Luciferase Assay Kit (NEB) according to the manufacturer’s instructions with luminescent detection on a SpectraMax M5 Microplate Reader (Molecular Devices ...
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bioRxiv - Genetics 2020Quote: ... and the library generated with a NEB Next Ultra II DNA Library Prep Kit for Illumina (NEB). The quality of the library was checked using an Agilent 2100 Bioanalyzer (Agilent High Sensitivity DNA kit) ...
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bioRxiv - Molecular Biology 2020Quote: ... The eluted DNA fragments were purified on a Monarch PCR & DNA Cleanup Kit (#T1030S, New England BioLabs and eluted with 26 μL DNAase and RNAase free water ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: WGS was performed using a modified NEBNext UltraII for DNA Library Prep kit (E7645L, New England BioLabs). Briefly ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... We cloned the gBlock insert into our vector library using the Quick Ligation Kit (New England BioLabs) and transformed and prepared DNA from this plasmid library as detailed above ...
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bioRxiv - Genetics 2019Quote: ... and used as input into the NEB Ultra II Directional RNA library kit for Illumina (NEB E7760) in two batches ...
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bioRxiv - Genetics 2019Quote: ... Sequencing libraries were prepared using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). The mRNA was selected by purifying polyA+ transcripts (NEBNext Poly(A ...
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bioRxiv - Biochemistry 2019Quote: ... Double stranded RNA reactions were performed using the HiScribe T7 High Yield RNA Synthesis Kit (NEB, #E2040S) and purified with the GeneJET RNA Purification Kit (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and a library was prepared using the NEBNext Ultra DNA library preparation kit (catalog number E7370L; NEB). Whole-genome sequencing of 150bp paired-end reads was generated on Illumina HiSeq2500 machine to a target coverage of 20X per sample ...
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bioRxiv - Microbiology 2019Quote: KLRC2 genotypes were determined by touchdown PCR using the Phusion High Fidelity PCR kit (New England Biolabs) using previously described methods40 ...
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bioRxiv - Genomics 2019Quote: ... Purified DNA sample was end-repaired (NEBnext ultra II end repair kit, New England Biolabs, MA, USA), cleaned up with 1x AMPure XP beads (Beckmann Coulter ...
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bioRxiv - Microbiology 2019Quote: Variants of UbiV and UbiU were constructed using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) according to the manufacturer’s specifications ...
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bioRxiv - Genomics 2019Quote: ... The second experimental replicate was prepared using the NEBNext Ultra II kit (New England Biolabs, Hitchin, UK) and sequenced using an Illumina NextSeq 500 platform ...
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bioRxiv - Plant Biology 2019Quote: ... ZymoResearch) and reverse-transcribed in the presence of random hexamers (LunaScript®RT SuperMix Kit, NEB Inc.) in 20 μl of total reaction volume containing 1 μg of total RNA and incubated at 55°C for 15 minutes following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... 2013) with probes synthesized using components based on the NEBlot Phototope Kit (New England BioLabs, Cat# N7550) and detected using components based on the Phototope-Star detection kit (New England BioLabs ...
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bioRxiv - Evolutionary Biology 2019Quote: ... RNA sequencing libraries were generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB #E7530). NEBNext Poly(A ...
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bioRxiv - Microbiology 2019Quote: ... The in vitro transcripts were produced using HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Libraries were then cloned using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB), following the recommended kit protocol and sequenced on a HiSeq2500 (Illumina).
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bioRxiv - Biochemistry 2020Quote: DNA was extracted from HLCs using the Monarch® Genomic DNA Purification Kit (New England BioLabs, T3010). A master mix was prepared using the Luna® Universal qPCR Master Mix (New England BioLabs ...
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bioRxiv - Molecular Biology 2021Quote: ... and final RNA-seq libraries generated using the NEBNext Ultra II Directional RNA Library Prep kit (NEB, E7760 and NEBNext Multiplex Oligos (NEB ...
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bioRxiv - Genomics 2019Quote: ... Fragmented DNA was used for library preparation using a NEBNext Ultra II Kit (NEB, Ipswich, MA USA) according to the manufacturer’s instructions for DNA end repair ...
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bioRxiv - Genetics 2021Quote: ... cDNA library was prepared with NEBNext Ultra II RNA library prep kit for Illumina (New England Biolabs), and the sequencing carried out using Illumina’s HiSeq-2500 sequencer with single-end mode and read length of 50 bp ...
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bioRxiv - Genetics 2020Quote: ... RNA was then reverse transcribed to cDNA using LunaScript RT SuperMix kit (Catalog # E3010L, New England BioLabs). w1118 flies might have partial or truncated transcripts of w+ ...
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bioRxiv - Genetics 2020Quote: ... Variants were generated using three-way Gibson cloning using NEB HiFi DNA Assembly Cloning Kit (NEB, E5520), using a NotI-AscI-digested and purified pENTR backbone (Thermo Scientific ...
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bioRxiv - Plant Biology 2019Quote: ... Libraries were prepared from the resulting mRNA with the NEBNext® Ultra™ II kit (NEB #E7770S). sRNA seq libraries were generated with the NEBNext® Multiplex Small RNA kit (NEB #E7300S) ...
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bioRxiv - Cancer Biology 2021Quote: ... following the manufacturer’s protocol A160141 v01 for library amplification using the NEBNext Ultra II kit (NEB E7645S).
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bioRxiv - Cell Biology 2020Quote: ... each sample received a different barcoded adapter included in the NEBNext Multiplex Oligos kit (New England BioLabs) to allow for multiplexed sequencing ...
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bioRxiv - Immunology 2021Quote: ... The adaptor-ligated DNA fragments were indexed with the NEBNext Multiplex Oligos for Illumina kit (NEB, E7335). Adapted and indexed libraries were quantified using a KAPA Library Quantification Kit (Roche ...
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bioRxiv - Immunology 2021Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Genomics 2020Quote: ... T7 transcription templates were transcribed using HiScribe T7 Quick High Yield RNA Synthesis Kit (New England Biolabs). Reactions were cleaned up using the MEGAClear Transcription Clean-up Kit (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... Products of PCR amplification were subjected to KLD reaction using Q5 Site-Directed Mutagenesis Kit (NEB, USA) and transformed into NEB 5-alpha Competent E ...
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bioRxiv - Microbiology 2021Quote: ... Truncated versions of this minigenome were generated using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) using the 3L5LEfull-Luc as a template.
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bioRxiv - Genomics 2021Quote: One reaction of NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7645) was used for 1 μg of input DNA ...
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bioRxiv - Systems Biology 2020Quote: ... Sequencing libraries were generated using NEBNext® Ultra™ DNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... Transcription of the RNA was carried out using a Highscribe T7 High-yield RNA synthesis kit (NEB), after linearisation of the DNA plasmid using HindIII ...
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bioRxiv - Cell Biology 2020Quote: ... These peptides were used as the substrate in a PKA kinase assay kit (New England Biolabs, #P6000S) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... two oligos (see Supplementary Table 3) were annealed and 5’ phosphorylated (T4 Polynucleotide Kinase kit, M0201S, NEB) as described previously (LentiGuide-Puro and LentiCRISPRv2) ...
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bioRxiv - Neuroscience 2020Quote: ... In vitro transcription of sgRNA template was performed using HiScribe Quick T7 kit (New England Biolabs #E2050S) following the manufacturer’s directions and incubating for 4 hr at 37°C ...
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bioRxiv - Bioengineering 2021Quote: ... were PCR-amplified and cloned into the pX601 plasmid using an NEBuilder HiFi DNA assembly kit (NEB).
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bioRxiv - Bioengineering 2021Quote: ... the PCR reaction was purified via Monarch® DNA Gel Extraction Kit (New England Biolabs® Inc.). Plasmids were constructed via the Hot Fusion Assembly Method (Fu et al. ...
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bioRxiv - Bioengineering 2021Quote: ... palustris gDNA which was obtained using Monarch® Genomic DNA Purification Kit (New England Biolabs® Inc.). A gentamicin selection marker was included in the expression cassette to force segregation into the endogenous plasmid ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Libraries for sequencing were prepared with the NEBNext Ultra II RNA Prep Kit (New England BioLabs, Inc.) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... Libraries were prepared using NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs E7770S) and the NEBNext Poly(A ...
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bioRxiv - Biochemistry 2020Quote: ... The Q5 site-directed mutagenesis kit was used in all site-directed mutagenesis experiments (New England Biolabs). The HRG-1 coding region was amplified from cDNA prepared from C ...
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bioRxiv - Biochemistry 2021Quote: ... R77E/A variants were produced by site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs), verified by sequencing ...