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Citations for New England Biolabs :
5001 - 5050 of 10000+ citations for Mouse Dachshund Homolog 1 DACH1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Eluted DNA was enriched by PCR using Phusion High-Fidelity PCR Master Mix kit (NEB, M0531S) and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1 ...
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bioRxiv - Cancer Biology 2024Quote: ... Library preparation was performed using the NEBNext Ultra RNA libray Prep Kit for Illumina (NEB, USA). RNA sequencing was performed using the Illumina NovaSeq platform ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA synthesis was performed using the Luna script RT Supermix kit (New England Biolabs, Cat#M3010L). Quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Cell Biology 2024Quote: ... cleaned up A-tailed DNA went through Adaptor ligation reaction using Quick Ligation Kit (NEB, M2200L) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... Libraries were produced with a NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) and sequenced on an Illumina NovaSeq 6000 platform using a 2 × 150 bp run protocol ...
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bioRxiv - Immunology 2024Quote: ... In vitro transcription was then done with the HiScribe T7 High Yield RNA Synthesis Kit (NEB), template DNA was removed with DNase I (NEB) ...
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bioRxiv - Microbiology 2024Quote: A NEBNext® Ultra™ II DNA Library Prep Kit (New England Biolabs, cat. no. E7645S) protocol for Illumina was used to prepare DNA libraries for sequencing ...
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bioRxiv - Bioengineering 2024Quote: ... and cDNA libraries were generated by RT-PCR using the ProtoScript II cDNA Synthesis Kit (BioLabs) according to the manufacturers’ protocols ...
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bioRxiv - Developmental Biology 2024Quote: ... dsDNA was converted to RNA with HiScribe™ T7 High Yield RNA Synthesis Kit (NEB, E2040S) and purified using Monarch® RNA Cleanup Kit (NEB ...
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High-resolution chromosome-level genome provides molecular insights into adaptive evolution in crabsbioRxiv - Genomics 2024Quote: ... Sequencing libraries were constructed using the NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA), following the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2024Quote: ... and libraries for Illumina sequencing were performed using the NEBNext Ultra II RNA kit (NEB, #E7770L). Quantification of the library was performed using dsDNA HS Assay Kit and Qubit 2.0 (Molecular Probes ...
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bioRxiv - Zoology 2024Quote: ... RT-qPCR was performed using the Luna One-step RT-qPCR kit (New England Biolabs, Germany) on a 384 well-plate with 3 technical replicates per biological replicate in CFX384 Touch Real-Time PCR (Bio-Rad ...
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bioRxiv - Synthetic Biology 2024Quote: ... A NEB Monarch® Plasmid Miniprep Kit was used for RT-DNA extraction (New England Biolabs). Following miniprep ...
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bioRxiv - Microbiology 2024Quote: ... UDI-barcoded RNAseq libraries were generated with the NEBNext Ultra II RNA Library Prep Kit (NEB). Each library was quantified with a Qubit (dsDNA HS kit ...
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bioRxiv - Immunology 2024Quote: ... Further modifications were performed by site-directed mutagenesis using the Q5 Site-Directed Mutagenesis Kit (NEB). All modifications to the 2X_pX458_pSpCas9(BB)-2A-GFP_SOX17_bKO plasmid (Addgene ...
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing libraries were prepared using NEBNext Ultra II DNA Library Prep Kit (New England BioLabs, E7645) following manufacture’s protocol ...
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bioRxiv - Immunology 2024Quote: ... and subsequently replaced with the tailed rpoS DNA fragment using the HiFi DNA assembly kit (NEB). Following the assembly reaction ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting PCR product was cloned into the pCDF4 vector using the Gibson Assembly kit (NEB) and the resulting plasmid was then used to generate ATG4-gRNA transgenic flies via injection into the y1 ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesised according to the protocols of the LunaScript RT Supermix Kit (New England Biolabs) and for qPCR amplification ...
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bioRxiv - Genetics 2021Quote: ... Total RNA was mixed with Reaction Mix 1 (9:1 of 12 uM NEB RT random 6N-S to 12 uM oligo-dT(20), 12uM dNTPs ...
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bioRxiv - Synthetic Biology 2021Quote: ... 5 µg of empty vector and 1 µg of purified amplicon were digested overnight with 1 µl of SphI-HF® (New England Biolabs, catalog no. R3182) in a total volume of 50 µl ...
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bioRxiv - Biophysics 2019Quote: ... the imaging chamber was incubated in blocking buffer (10 mM Tris HCl pH 7.5, 50 mM NaCl, 1 mg/mL BSA [NEB], 1 mg/mL tRNA [Ambion]) for 1 h ...
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bioRxiv - Bioengineering 2022Quote: ... and 15ul BseYI-digested product were run side-by-side on gel (1% agarose gel in TAE buffer, 1 kb ladder (NEB, Cat. No. / ID: N3232L), most gels run at 115V for 40 minutes) ...
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bioRxiv - Genomics 2023Quote: ... Lysed cells were then diluted 10 time in several tubes containing 450 μl of digestion mix (1X NEB 1 buffer, 1% Triton X-100). 100 units of HpaII were added and the tubes were incubated for 2 hours at 37°C ...
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bioRxiv - Genetics 2021Quote: ... T7 endonuclease 1 assay was performed following manufacturer’s protocol (NEB).
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bioRxiv - Developmental Biology 2021Quote: ... and mounted in 1% low melting point agarose (LMP) (Biolabs) within a 1mm diameter glass capillary ...
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bioRxiv - Genomics 2020Quote: ... 1 μl (10 U/μl) of T4 polynucleotide kinase (NEB) and 1 μl of 10 mM dNTPs in a final volume of 50 μl at 24°C for 30 min ...
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bioRxiv - Genomics 2020Quote: ... 1 μl (3 U/μl) of T4 DNA polymerase (NEB), 1 μl (10 U/μl ...
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bioRxiv - Physiology 2022Quote: ... anti-MBP (1:10,000, catalog no. E8032S, New England Biolabs); anti-CCNA2 (1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... Excess adapter was digested by adding 1 µl RecJf (NEB) and 1 µl 5’deadenylase (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... both proteins were deglycosylated by PNGase F (NEB, 1:50) overnight at 37°C in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... rabbit anti-GFP (1:1000; TP401, Torrey Pine Biolabs, RRID:AB_10013661), mouse anti-GFP (1:1000 ...
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bioRxiv - Genomics 2020Quote: ... and 1 unit of poly(A) polymerase (New England Biolabs) and was incubated at 42°C for 1 hour followed by enzyme inactivation at 95°C for 5 minutes ...
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bioRxiv - Genomics 2020Quote: ... 5′ adapter ligation with T4 RNA Ligase 1 (NEB, M0204L). The resulting RNA fragments were used for a reverse transcription reaction using Superscript III Reverse Transcriptase (Life Technologies ...
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bioRxiv - Genomics 2020Quote: ... 0.1mM dNTPs and 1 U/μl USER enzyme (NEB, USA). 75μl of the mix was added and incubated for 3h at 37°C ...
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bioRxiv - Genomics 2019Quote: ... 2 μl of proteinase k (800 units ml-1, NEB) were added and reacted for 1 hour at 50°C ...
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bioRxiv - Molecular Biology 2019Quote: ... Each PCR (20 μl) contained 1× standard PCR buffer (NEB), 1 U of Taq polymerase (NEB) ...
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bioRxiv - Cancer Biology 2019Quote: ... and dNTPs in 1× T4 DNA ligase reaction buffer (NEB), followed by dATP-addition with Klenow ...
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bioRxiv - Microbiology 2020Quote: ... 1× Phusion HF buffer (New England Biolabs GmbH, Schwalbach, Germany) with 1.5 mM MgCl2 ...
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bioRxiv - Molecular Biology 2019Quote: ... 2% DMSO and 1 U Phusion high fidelty polymerase (NEB) in 1x buffer provided by the manufacturer ...
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bioRxiv - Genomics 2019Quote: ... and various amounts of the 1 kb ladder (NEB#N3232s) were run on 1% Agarose gel.
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bioRxiv - Cell Biology 2019Quote: ... 1% NP40 and PNGase-F (50 U/μl: NEB#P0704S).
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bioRxiv - Genomics 2019Quote: ... 34 µl of H2O and 1 µl of PstI (NEB) were added and restriction digestion was performed at 37°C for 4 h ...
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bioRxiv - Genomics 2021Quote: ... and 1 µl of PNK (NEB M0201, 10,000 units/ml) and incubated at 37 °C for 20 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... were ligated using 1 μl Quick T4 DNA Ligase (NEB) and DNA fragments were amplified by PCR with NEXTFlex primers (Primer 1 - 5’-AATGATACGGCGACCACCGAGATCTACAC ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 µl T7 Endonuclease I (1 U/ul, M0302S, NEB) was added and incubated at 37°C for 1 hr ...
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bioRxiv - Genomics 2020Quote: ... 1 µl T4 RNA Ligase 2 truncated K227Q (200U; NEB)] was added ...
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bioRxiv - Genomics 2020Quote: ... 1 µl Calf Intestinal alkaline Phosphatase (10 U; CIP, NEB) or quick CIP (10 U ...
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bioRxiv - Cell Biology 2020Quote: ... 2700 units mL−1 murine RNase inhibitor (New England Biolabs), and 0.1 M Tris-HCl (pH 8.0)).81 Primers consisted of a poly-T sequence to trap mRNA molecules ...
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Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: ... and 1 U/µL Murine RNAse Inhibitor (NEB, Ipswitch, MA). Labeling reactions were incubated for 2 h at 37 °C ...