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Citations for New England Biolabs :
451 - 500 of 2304 citations for Rnase 3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... or RNase If (1:100 diluted, New England Biolabs) in buffer 3 (100 mM NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 U/μL murine RNAse Inhibitor (New England Biolabs), 62.5 nM fluorescent reporter (/5FAM/rUrUrUrUrUrU/IABkFQ/ ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with murine RNAse Inhibitor (1 U/μL, NEB), fluorescent reporter (500 nM ...
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bioRxiv - Microbiology 2023Quote: ... 4 μl of murine RNase inhibitor (New England Biolabs), and 40 μg (HEK293T and U2OS cells ...
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bioRxiv - Microbiology 2023Quote: ... 1 μl of murine RNase inhibitor (New England Biolabs), 1 μl of 10 mM dNTP mix (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 U/μl of DNase I (RNase free) (NEB) was added to each reaction followed by 30 min incubation at 37°C for the elimination of the DNA templates ...
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bioRxiv - Neuroscience 2023Quote: ... containing 0.4 U/ml RNAse inhibitor (New England Biolabs) and were immediately transported to the sequencing facility on ice for further processing.
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bioRxiv - Genomics 2023Quote: ... 100 μL of RNAse Inhibitor (New England BioLabs M0314L) was added to 250 μL of Imaging Buffer Activator (PN 203000015 ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 µl of 20 mg/ml RNase A (NEB) was added ...
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bioRxiv - Molecular Biology 2024Quote: ... and 5.5 mL of RNase If (NEB, Cat. M0243L) per million cells for 15 seconds and mixed by flicking ...
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bioRxiv - Genetics 2019Quote: ... Hi-C DNA was amplified using Index Primers set 1 (NEB, E7335S). The Hi-C libraries were purified with AMPure XP beads (Beckman Coulter ...
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bioRxiv - Immunology 2021Quote: ... and the fragment was assembled using Hi-Fi DNA Assembly Mix (NEB) into the donor vector pADN171XS7 ...
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bioRxiv - Microbiology 2021Quote: ... His-MBP-MirA were first isolated using amylose bead affinity chromatography (NEB) in conditions specified by the manufacturer ...
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bioRxiv - Plant Biology 2021Quote: ... The homology plasmids were assembled using HI-Fi DNA Assembly Mastermix (NEB) with four PCR-generated DNA fragments (see Table S2 for primers) ...
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bioRxiv - Molecular Biology 2019Quote: ... purified and then cleaved with Xho I and Bam HI (both NEB). After further purification ...
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bioRxiv - Microbiology 2021Quote: ... Then the His-tag was cleaved by incubation with TEV protease (NEB) for 18 h at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... and then ligated by Hi-Fi DNA assembly (New England Biolabs Inc.). Irgb6-WT and Irgb6-T95D were expressed and purified as described (Saijo-Hamano et ...
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bioRxiv - Biochemistry 2023Quote: ... was cloned into pUC19 by Gibson cloning (Hi-Fi cloning system, NEB) to replace the yeast sequence:
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bioRxiv - Genomics 2023Quote: ... and incubated at 37°C for 1 hour with RNase A/T1 (Thermo, 1/20 volume) and RNase H (NEB, 1/50 volume). The DNA was then purified again.
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bioRxiv - Cell Biology 2024Quote: ... and treated for 10 min at RT by RNase III enzyme (2 U of RNase III (Short Cut, New England BioLabs, Massachusetts, USA, M0245S) with 20 mM MnCl2 in 1× reaction ShortCut buffer) ...
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bioRxiv - Biophysics 2020Quote: The transient transfection of HEK293 cells was performed67 using the TransFectin Lipid Reagent (Bio-Rad) (New England Biolabs). Regularly ...
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bioRxiv - Biophysics 2020Quote: The transient transfection of HEK293 cells was performed67 using the TransFectin Lipid Reagent (Bio-Rad) (New England Biolabs).
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bioRxiv - Cell Biology 2023Quote: ... HEK293 libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Cat. #E7770) or NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (#E7760) ...
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bioRxiv - Microbiology 2020Quote: ... 3 μL Klenow fragment (3’→5’ exo-) (NEB), and 9 μL of DEPC H2O to each reaction and incubating at 37 °C for 30 min ...
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bioRxiv - Cancer Biology 2021Quote: ... with a murine RNase inhibitor (cat. #M0314, New England Biolabs) followed by cDNA synthesis using a High-Capacity cDNA Reverse Transcription kit (cat ...
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bioRxiv - Genetics 2020Quote: ... RNA was digested enzymatically with RNAse H (NEB, Ipswich, USA) following the protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... a mix containing 2 μl of RNAse H (NEB, #M0297S), 1 μl of E ...
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bioRxiv - Molecular Biology 2021Quote: ... and 0.5 µL of RNase H (New England Biolabs, M0297S)) to the sample and incubating at 4°C for 1 s ...
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bioRxiv - Molecular Biology 2021Quote: ... 2.5 mM MgCl2) and RNA digested with RNase H (NEB) and 1 uL of RNAse T1 (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... in the presence of 20 U RNase inhibitor (M0314L, NEB) for 60 min at 37 °C ...
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bioRxiv - Genomics 2020Quote: ... 0.5% Sodium deoxycholate and 1.1% Murine Rnase Inhibitor (M0214L, NEB). Cells were allowed to lyse for 15 min on ice ...
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bioRxiv - Genomics 2020Quote: ... RNase inhibitor was added to the cell suspension (NEB #M0314S).
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bioRxiv - Cancer Biology 2019Quote: ... 200U RNase inhibitors and 200U M-MuLV reverse transcriptase (NEB). All the reagents were mixed and incubated at 42°C for 1 hour followed by 65°C for 20 minutes ...
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bioRxiv - Cell Biology 2019Quote: ... Bound material was washed five times and RNase H (NEB) was added for 30 min at RT to elute RNA-chromatin complexes ...
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bioRxiv - Cell Biology 2020Quote: ... 2700 units mL−1 murine RNase inhibitor (New England Biolabs), and 0.1 M Tris-HCl (pH 8.0)).81 Primers consisted of a poly-T sequence to trap mRNA molecules ...
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bioRxiv - Biochemistry 2021Quote: ... and RNase If were purchased from New England Biolabs (NEB).
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Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: ... and 1 U/µL Murine RNAse Inhibitor (NEB, Ipswitch, MA). Labeling reactions were incubated for 2 h at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... 125 to 75 U/ 5 μg RNase B (Cat.P7817S, NEB) and fetuin (Cat ...
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bioRxiv - Microbiology 2020Quote: ... 1μL of RNase H (New England BioLabs, Ipswich, MA, USA) was added to the reaction and incubated at 37°C for 20 min ...
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bioRxiv - Plant Biology 2021Quote: ... RNA was treated with RNase-Free DNase (New England Biolabs) and reverse-transcribed following manufacturer’s recommendations (The ProtoScript Taq RT-PCR Kit ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then treated with 10U of RNase H (NEB), in PBS 1x for 1 hour at RT ...
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bioRxiv - Cell Biology 2021Quote: ... Then 2-5 unites of RNase H (New England Biolabs) in 5 µl of 1x RNase H buffer were added to the annealed RNA-chimeric oligo mixture and the RNase H cleavage reaction was performed at 37o C for 1 h ...
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bioRxiv - Bioengineering 2022Quote: 1 U (0.5 μl) RNaseIII (NEB Short Cut RNase III), was combined with 1 μl of 10X reaction buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1 μl of 5 units/µl RNase H (NEB) were added and samples were incubated at 37°C for 30 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... and 900 U of Murine RNase Inhibitor (New England Biolabs), and cell sample passed 10-15 times through a 27-gauge needle attached to a 25 ml syringe in order to disrupt the pellet and shear genomic DNA ...
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bioRxiv - Microbiology 2022Quote: ... 1 μl 40 U μl-1 RNase Inhibitor (NEB, M0314), and 1 μl T4 RNA ligase 2 (truncated K227Q ...
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bioRxiv - Biochemistry 2019Quote: ... then 1ul of RNase If (50,000units/ml) (NEB, Ipswich, MA) and 4ul of 10X NEB buffer 3 (NEB ...
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bioRxiv - Synthetic Biology 2019Quote: ... 0.5 μL (20 U) of RNase inhibitor (New England Biolabs), and 2.5 μL of TGIRT-III reverse transcriptase (InGex ...
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bioRxiv - Molecular Biology 2021Quote: ... or DNase I (RNase-free, New England Biolabs, Inc, MA), followed by phenol extraction ...
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bioRxiv - Genomics 2021Quote: ... with oligo-dT20 and RNAse H (New England Biolabs, USA). Full length coding sequences of CYP9K1 were amplified separately from cDNA of 10 mosquitoes using the Phusion HotStart II Polymerase (Thermo Fisher ...