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Citations for New England Biolabs :
451 - 500 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... thermophilus extracts or PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs, Ipswich, MA, USA) and the reactions were further incubated at either 50 °C or 37 °C for another 4 h in the case of T ...
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bioRxiv - Immunology 2020Quote: ... Plasmid digestions were performed following manufacturer recommendations and generally subjected to Antarctic phosphatase (New England Biolabs # M0289S) treatment to prevent potential re-annealing ...
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bioRxiv - Genomics 2019Quote: ... with the efficiency of methylation assessed by digestion using HpaII and HhaI methylation-sensitive restriction enzymes (NEB). The effect of SOX9 on enhancer activity was assessed by transfection with a SOX9 overexpression plasmid (pUT-FLAG-SOX9 ...
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bioRxiv - Cell Biology 2021Quote: ... 15 μl of amplified DNA was used for EcoRI and BmrI restriction enzyme digestion (New England Biolabs). DNA products were separated by gel electrophoresis on a 3% agarose gel ...
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bioRxiv - Microbiology 2021Quote: ... the genomic clone was released from the bacterial plasmid by digestion with Pac I (New England Biolabs), and 1 μg of linearized DNA was transfected into A549 cells using Effectene Transfection Reagent (Qiagen ...
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bioRxiv - Genetics 2019Quote: ... we carried out second digestion with 20 units of DpnII restriction enzyme (New England Biolabs, Cat#R0543). Then the chromatin was again self-ligated with 12.5 units of T4 DNA ligase (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... The linearized viral backbone was created by restriction digestion using the following enzymes: HpaI and SalI (NEB). All inserts were generated with PCR amplification using phusion DNA polymerase (Fischer Scientific) ...
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bioRxiv - Immunology 2021Quote: ... Plasmid clones with correct insert were further confirmed by restriction digestion with respective (New England Biolabs, Inc.) restriction enzymes before being selected for the subsequent transfection experiment ...
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bioRxiv - Cell Biology 2020Quote: ... The integrity of GTSE1-14A BAC was checked by performing restriction digestion with NotI (New England Biolabs) and comparing the obtained profile with that of the GTSE1 WT BAC.
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bioRxiv - Microbiology 2021Quote: ... and digestion of a pEF1a-IRES vector (www.takarabio.com, Cat. # 631970) with NheI-HF and SalI-HF (NEB). Fragments were ligated with the Quick Ligation kit (NEB).
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bioRxiv - Molecular Biology 2019Quote: RNA digestion of 20 OD260nm of cell extracts were performed by using 1 unit of Xrn1 (Biolabs) in NEB buffer 3 at 25°C during 30 min unless otherwise indicated ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids were isolated from individual clones and confirmed by digestion with HindIII (New England Biolabs, Cat# R0104) and the resulting plasmid pSB-pCTR4 was used to amplify the final FKS1 promoter replacement cassette.
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bioRxiv - Cell Biology 2022Quote: ... where the GFP-LGN sequence was removed by restriction digestion with Bsu36i and PspOMI (New England Biolabs). The primers were 5’-CCGACCTGAGGAAGGGAG3-3’ (forward ...
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bioRxiv - Genomics 2023Quote: ... For tissue clearing, samples were incubated in digestion premix (Vizgen, #20300005, USA) with RNase inhibitor (NEB, USA) for 1 h at 23 °C ...
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bioRxiv - Microbiology 2023Quote: ... restriction digestion and ligation steps were performed following the instructions provided by the manufacturer (New England Biolabs). The genomic DNA was digested using the restriction enzymes Nco□ ...
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Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: ... into the piggyBac backbone from Addgene plasmid # 126029 (Schertzer et al. 2019) by digestion with SpeI and EcoRI followed by Gibson Assembly (NEB).
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Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: ... in the piggyBac backbone from Addgene plasmid # 126028 (Schertzer et al. 2019) by digestion with SfiI and BglII followed by Gibson Assembly (NEB). Oligonucleotides used for cloning are in Supplemental Table S1 ...
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bioRxiv - Molecular Biology 2023Quote: ... or ClaI and PacI restriction sites pairs of CircRNA Mini Vector by restriction digestion (New England Biolabs) and DNA ligation using T4 DNA Ligase (New England Biolabs).
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bioRxiv - Microbiology 2024Quote: ... Single-construct plasmids expressing A-subunits were constructed via restriction digestion (NdeI and PstI, New England Biolabs) and ligation using pBAD24 (Amp+ ...
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bioRxiv - Biochemistry 2023Quote: ... PCR reactions were purified using GeneAid and ∼100 ng of DNA subjected to digestion by NaeI (NEB). Clones that showed no apparent digestion product (due to the modification of the NaeI restriction site ...
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bioRxiv - Molecular Biology 2023Quote: ... 150 ng of deaminated DNAs were digested to nucleosides with the Nucleoside Digestion Mix (NEB, Ipswich, MA) following manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2019Quote: ... The assay was performed using the PURExpress In Vitro Protein Synthesis Kit (E6800S New England Biolabs, Inc.), following manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: The TF samples were expressed by using a PURExpress In Vitro Protein Synthesis Kit (New England BioLabs) and analyzed in duplicate on two different PBM arrays (HK and ME ...
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bioRxiv - Genomics 2022Quote: ... The TF sample was expressed by using a PURExpress In Vitro Protein Synthesis Kit (New England BioLabs) and analyzed in duplicate on two different PBM arrays (HK and ME ...
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bioRxiv - Microbiology 2024Quote: ... 1.4 μM of each purified mRNA was used in PURExpress in vitro Protein Synthesis Kit (NEB#E6800) in 10 μl reaction in 384 well Small Volume black plate (Grenier 788 096) ...
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bioRxiv - Cell Biology 2020Quote: ... MNase (non-specific DNA digestion) used MNase buffer and 2 μL of enzyme (20 units/μL) PvuII (NEB), AluI (NEB ...
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bioRxiv - Cell Biology 2020Quote: ... Digestion was performed overnight using 0.5-2.5 μg of this genomic DNA and restriction enzyme DpnI (NEB, R0176) and then heat-killed for 20 minutes at 80°C ...
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bioRxiv - Biochemistry 2020Quote: ... The relative abundance of oligomannose glycans was measured by digestion with Endoglycosidase H (Endo H; New England Biolabs). Digestion was performed overnight at 37 degrees ...
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bioRxiv - Biochemistry 2019Quote: ... which followed digestion of affinity tag and deglycosilation by TEV protease and MBP-fusion endoglycosidase (New England Biolabs), respectively ...
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bioRxiv - Genomics 2021Quote: Genomic DNA and synthetic oligonucleotides were digested to nucleosides by treatment with the Nucleoside Digestion Mix (NEB #M0649S) at 37 °C for 3 h ...
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bioRxiv - Immunology 2022Quote: T7 endonuclease I digestion: 200 ng of purified DNA amplicons were annealed with 1X NEBuffer 2 (NEB, # B7002S) and total volume was brought up to 19 uL ...
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bioRxiv - Cell Biology 2022Quote: ... before digestion-ligation with the gRNA-pScaffold-H1 using FastDigest Esp3I and T7 Ligase (M0318L, New England Biolabs).
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bioRxiv - Neuroscience 2021Quote: ... nuclei were resuspended in 200uL digestion buffer with 4 uL protease (an equal volume of protease K (NEB) was substituted if the manufacturer-provided protease was exhausted ...
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bioRxiv - Developmental Biology 2021Quote: ... Genomic targeting efficiency for each gRNA was determined through annealing and digestion with T7 Endonuclease I (NEB: #M0302) of PCR products flanking the INPP5E D447N target site ...
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bioRxiv - Developmental Biology 2021Quote: ... The orientation of the insert after linearization of the vector with SpeI restriction digestion (New England Biolabs, Inc.) was determined by Sanger sequencing ...
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bioRxiv - Immunology 2020Quote: ... The relative abundance of oligomannose glycans was measured by digestion with Endoglycosidase H (Endo H; New England Biolabs). Digested glycans were cleaned using a PVDF protein-binding membrane (Millipore ...
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bioRxiv - Microbiology 2022Quote: ... or by digestion with the indicated restriction enzymes followed by ligation using T4 DNA ligase (New England Biolabs). All strain mutations and plasmid inserts were confirmed by sequencing.
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bioRxiv - Zoology 2019Quote: ... The pDmelOR vector was sequenced after digestion with XhoI and PvuI-HF (Cat. Nr. R3150, New England Biolabs); constructs with inserted Or47a and Or56a genes were cut with XhoI and NaeI (Cat ...
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bioRxiv - Genomics 2020Quote: ... Supernatant was transferred to a new tube and incubated with DNA Digestion Buffer (20 μl DNaseI (NEB M0303), 0.5 mM CaCl2 ...
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bioRxiv - Biochemistry 2019Quote: ... The 6xHIS-TEV-KID fragment (NdeI and XhoI digestion) was cloned into the pMALc5x vector (New England Biolabs) via NdeI and SalI ...
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bioRxiv - Molecular Biology 2021Quote: ... by AgeI and EcoRI digestion and ligation of 5’-phosphorylated DNA duplexes using T4 DNA ligase (NEB, #M0202). Target plasmids expressing 0 and 480 CTG repeats in the context of DMPK exons 11-15 (DMPKS and DT480 ...
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bioRxiv - Biochemistry 2021Quote: ... 50 μl of lysed cells were aliquoted to 8 tubes containing 450 μl of digestion mix (1× NEB 1 buffer ...
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bioRxiv - Cell Biology 2020Quote: 50 μl of lysed cells were aliquoted to 8 tubes containing 450 μl of digestion mix (1x NEB 1 buffer ...
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bioRxiv - Genomics 2019Quote: ... Digestion was performed overnight using 0.5-2.5 μg of this genomic DNA and restriction enzyme DpnI (NEB, R0176) and then heat-killed for 20 minutes at 80°C ...
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bioRxiv - Bioengineering 2022Quote: The plasmid backbone was linearized by sequential digestion with SmaI and SpeI (New England Biolabs, Ipswich, MA, USA), and gel purified from 0.8% SeaPlaque GTG Agarose (Lonza ...
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bioRxiv - Cell Biology 2022Quote: ... After verification by Sanger sequencing the TNY1 cDNA fragment was released by digestion with NdeI and XhoI (NEB) and inserted into vector pET28a digested with NdeI and XhoI ...
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: ... The PSMB7 single mutant clones were verified by restriction enzyme digestion (EcoRV and HindIII from New England BioLabs) followed by Sanger sequencing ...
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bioRxiv - Microbiology 2022Quote: ... The digestion product was then treated with Antarctic phosphatase for 1 hour at 37°C (New England Biolabs). PCR products were purified using the Zymogen Clean and Concentrator PCR-cleanup kit (Zymo Research ...
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bioRxiv - Neuroscience 2022Quote: ... 4 and subjected to T7E1 digestion in a 20 ul reaction according to the manufacturer’s protocol (NEB cat.M0302). Digestion products were resolved on 8% acrylamide/bis TBE gel and visualized with EtBr.
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bioRxiv - Synthetic Biology 2023Quote: ... We performed the isolation of 16 random clones and triple restriction enzyme digestion using BsrGI-HF (NEB #R3575S), ClaI (NEB #R0197S) ...