Labshake search
Citations for New England Biolabs :
451 - 500 of 1702 citations for IL 10 Human HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Systems Biology 2023Quote: ... 0.4 μL 10 mM dNTP solution (New England Biolabs), 4 μL 5× phusion HF buffer (Thermo Scientific) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1% SDS and 10 U/ml Proteinase K (NEB), followed by an 18-hour incubation at 56 °C with vigorous shaking ...
-
bioRxiv - Genomics 2024Quote: ... 10 µl of 2% BSA (NEB, B9000S, 0.01% final) and 930 µl of nuclease-free water and supplemented with 1U/ml Protector RNase inhibitor (Millipore Sigma ...
-
bioRxiv - Neuroscience 2023Quote: ... Protein A beads (10 µl, New England Biolabs, #S1425) were washed twice in coIP-solution and consecutively incubated for 1 hour at 4°C with an anti-synaptopodin antibody (rabbit anti-Synaptopodin ...
-
bioRxiv - Molecular Biology 2023Quote: ... 10 µl of 20 mg/ml RNase A (NEB) was added ...
-
bioRxiv - Molecular Biology 2024Quote: ... 10 mM vanadyl-ribonucleoside (VRC; #S1402S, New England Biolabs) overnight at 37°C ...
-
bioRxiv - Biochemistry 2023Quote: ... Broad Range (10-250 kDa) (New England Biolabs #P7719S) was used as a protein standard ...
-
bioRxiv - Biochemistry 2024Quote: ... and 10 U T4 polynucleotide kinase (New England Biolabs) in a final volume of 10 µL ...
-
bioRxiv - Synthetic Biology 2024Quote: ... NEB 10-beta cells (New England Biolabs, Ipswitch, MA) were used for cloning and testing of all constructs ...
-
bioRxiv - Genetics 2022Quote: Candidate enhancers were amplified from human genomic DNA using Q5 Hot-Start High-Fidelity DNA polymerase (New England Biolabs) and directionally cloned into the pGL3-Promoter plasmid (pGL3-P ...
-
bioRxiv - Biochemistry 2020Quote: ... Human β2AR was fused at its N-terminus to the ACP- or Halo7-tag protein (NEB and Promega, respectively) at the cDNA level ...
-
bioRxiv - Microbiology 2019Quote: ... in conjunction with the NEBNext® rRNA Depletion Kit for Human/Mouse/Rat (New England BioLabs, Ipswich, MA, USA) and the MICROBExpress kit (Invitrogen ...
-
bioRxiv - Synthetic Biology 2022Quote: ... sgRNA expression plasmids were constructed via digesting the sgRNA backbone plasmids that contained a human U6 promoter and a Cas-nuclease’s corresponding sgRNA scaffold sequences using BsmB I or Bbs I endonuclease (NEB) and then ligated the oligonucleotide duplexes into this cut backbone ...
-
The absence of C-5 DNA methylation in Leishmania donovani allows DNA enrichment from complex samplesbioRxiv - Molecular Biology 2020Quote: ... Leishmania DNA (0.0017 ng/μL) was then enriched from the human DNA (25ng/μL) using NEBNext Microbiome DNA Enrichment Kit (NEB) according to manufacturer instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... The cDNAs encoding human elongation factor eEF2 were amplified by PCR using Q5 High-Fidelity 2X Master Mix (NEB) with primers (forward primer ...
-
bioRxiv - Cell Biology 2022Quote: mRNA was enriched from 100ng DNase treated total RNA using the NEBNext rRNA depletion Kit (human, mouse, rat, NEB) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... elegans osgn-1 (F30B5.4a, WormBase WS265) and human OSGIN1 were amplified by PCR using Phusion DNA polymerase (NEB, M0530L), according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... for 1 h at room temperature before being incubated with 1.0 µg/mL of recombinant human histone H3.1 (New England Biolabs, #M2503S) for 1 h at room temperature ...
-
bioRxiv - Genomics 2022Quote: ... OMNI ATAC-Seq was performed as previously described with 320,000 human CD4+ T cells (in total) in batches of 100,000 cells per reaction (NEB #M0544S).(26 ...
-
bioRxiv - Genetics 2022Quote: ... The sequences of HS5 and NRE were PCR amplified from human genomic DNA using Phusion high fidelity polymerase (NEB). Hg38 genome coordinates and sequences of primers used ...
-
bioRxiv - Immunology 2023Quote: ... A 140-500 ng aliquot of total RNA was rRNA depleted using NEB’s Human/Mouse/Rat RNAse-H based Depletion kit (New England BioLabs). Following rRNA removal ...
-
bioRxiv - Cancer Biology 2024Quote: ... Specific mutations in human PSEN1 were obtained by Q5 site-directed mutagenesis of the pMSCV-puro vector containing wild type human PSEN1.26 We designed primers for Q5 site-directed mutagenesis (New England Biolabs) with NEBaseChanger.neb.com and performed site-directed mutagenesis according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2020Quote: ... and Illumina sequencing adaptor ligation (New England BioLabs, E6000B-10). Libraries were then indexed and PCR amplified (10 cycles ...
-
bioRxiv - Genetics 2021Quote: ... and 10 U polynucleotide kinase (New England Biolabs, Beverly, MA) as instructed by the manufacturer ...
-
bioRxiv - Genomics 2020Quote: ... 10 μL 10x T4 DNA Ligase Reaction Buffer (NEB B0202S), 0.5 μL 200x BSA (NEB ...
-
bioRxiv - Genomics 2021Quote: Lambda exonuclease: 10 U λ exonuclease (New England Biolabs, M0262) in 60 μl 1×λ exonuclease reaction buffer was added and incubated at 37°C for 30 minutes.
-
bioRxiv - Genomics 2020Quote: ... 1 μl (10 U/μl) of T4 polynucleotide kinase (NEB) and 1 μl of 10 mM dNTPs in a final volume of 50 μl at 24°C for 30 min ...
-
bioRxiv - Neuroscience 2019Quote: ... and 10 mM vanadyl-ribonucleoside complex (S1402S, New England BioLabs) with probes at final concentration of 1 ng/μl ...
-
bioRxiv - Genomics 2019Quote: ... DpnII buffer and DpnII (10 U, New England Biolabs #R0543L) were added in a total volume of 50 µl and incubated at 37 °C for 1 h ...
-
bioRxiv - Genetics 2021Quote: ... 10 uL of 10U/uL AluI restriction enzyme (NEB R0137S) was added to each sample ...
-
bioRxiv - Genomics 2021Quote: ... 120 μL 10×NEB T4 DNA ligase buffer (NEB B0202), 100 μL of 10% Triton X-100 ...
-
bioRxiv - Genetics 2020Quote: ... we added 10 µL of Shrimp Alkaline Phosphatase (NEB #M0371L) and incubated at 37°C for 1 h ...
-
bioRxiv - Microbiology 2021Quote: ... and 10 mM Ribonucleoside-Vanadyl Complex (NEB, Ipswich, MA, USA) on ice ...
-
bioRxiv - Genomics 2020Quote: ... 1 µl Calf Intestinal alkaline Phosphatase (10 U; CIP, NEB) or quick CIP (10 U ...
-
bioRxiv - Biochemistry 2020Quote: ... and 50 µL of 10 mM dNTPs (New England BioLabs), 100 µL 0.1 M DTT ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were initially incubated with SNAPcell block (10 µM; NEB) diluted in culture media for 20 min ...
-
Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... 4 μl 10× T4 RNA ligase buffer (New England Biolabs), 4 μl T4 RNA ligase ...
-
bioRxiv - Systems Biology 2020Quote: ... 22 μL of 10 mM/each of four dNTPs (NEB), 5.5 μL of TSO primer (100 μM) ...
-
bioRxiv - Microbiology 2021Quote: ... coli (NEB 10-beta electrocompetent cells, New England BioLabs C3020K) and plated at a target bottleneck of 50,000 variants per duplicate library ...
-
bioRxiv - Cell Biology 2022Quote: ... and 10 mM adenosine triphosphate (New England Biolabs, United States) was added to the 17 μl solution from the first step ...
-
bioRxiv - Biochemistry 2022Quote: ... coli strain NEB 10-β competent cells (New England Biolabs) and plated on LB agar containing 50 μg/mL kanamycin ...
-
bioRxiv - Genomics 2022Quote: ... The product was transformed into 10-beta Electrocompetent E.coli (NEB), by electroporation with 2.0kV ...
-
bioRxiv - Microbiology 2022Quote: ... coli (NEB 10-beta electrocompetent cells, New England BioLabs C3020K) and plated at a target bottleneck of 100,000 variants per duplicate library ...
-
bioRxiv - Molecular Biology 2020Quote: ... 5% DMSO and 10 units of λ exonuclease (NEB, M0262S) at 37°C for 30 min ...
-
bioRxiv - Biochemistry 2021Quote: 10 ug of protein extract were digested with PNGaseF (NEB) according to manufacturer instructions ...
-
bioRxiv - Cancer Biology 2021Quote: ... 10 µl of 2X Phusion Master Mix (NEB, Cat#M0531L), and 3.6 µl H2O (20 µl reaction) ...
-
bioRxiv - Genomics 2022Quote: ... Each reaction contained 10 µL of Q5 Reaction Mix (NEB), 10 µL of Q5 GC Enhancer Buffer (NEB) ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... and 10 μl of 5 U/μl Klenow (NEB M0210)) and incubating 1h at 37ºC with rotation ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... 2.5 μl of T4 PNK (10 U/μl, NEB M0201) and 0.5 μl of Klenow DNA polymerase (5 U/μl NEB M0210) ...
-
bioRxiv - Microbiology 2022Quote: ... with the addition of 10 mg BSA (New England BioLabs). We added 0.1 femtomole 515F and 806R which do not have the barcodes and adapters to overcome initial primer binding inhibition because the majority of the primers do not match the template priming site ...