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Citations for New England Biolabs :
451 - 497 of 497 citations for Fatty acids tall oil mixed with oleic acid sulfated potassium salts since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... 2 µl preramp PCR product was mixed with 48 µl tagging PCR mix (10 µl 5x phusion HF buffer (NEB); 1 µl Phusion Hot Start II DNA Polymerase (2U/ µl ...
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bioRxiv - Immunology 2022Quote: ... Sample index PCR: 2 µl tagging PCR product was mixed with 18 µl PCR mix (4 µl 5x phusion HF buffer (NEB); 0.4 µl Phusion DNA Polymerase (NEB) ...
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bioRxiv - Cell Biology 2024Quote: ... The inserts and the backbone were mixed in a 3:1 ratio and incubated with the 2x NEBuilder HiFi DNA assembly enzyme mix (New England Biolabs) for 1h at 50°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 22.5 µl of first-strand cDNA was mixed with 2.5 µl of Native Barcode (EXP-NBD104) and 25 µl of Blunt/TA Ligase Mix (NEB, M0367S) and incubated at room temperature for 10 min ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 µM of sgRNA scaffold and a RPA1-specific forward primer and were mixed in 1× HiFi reaction buffer with MgCl2 and 1 unit Phusion High-Fidelity DNA Polymerase (New England BioLabs), 25 µl total volume ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µl of genomic DNA (∼100 ng) was mixed with 8 µl of 1x Cutsmart buffer (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µl of genomic DNA (∼100 ng) was mixed with 8 µl of 1x Cutsmart buffer (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Synthetic Biology 2024Quote: ... A fraction (generally 6 µL) of the eluate was mixed with an equal volume of 6x purple gel loading dye (NEB) and loaded in 1% agarose gel with ethidium bromide ...
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bioRxiv - Synthetic Biology 2024Quote: ... The double stranded products were mixed with IVT reagents from the HiScribe® T7 High Yield RNA Synthesis kit (NEB) and incubated at 37 °C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... was prepared by injecting 250 μl of Imaging Buffer activator (ref. 20300022, Vizgen) mixed with 100 μl of RNase inhibitor (ref. M0314L, NEB) into the cartridge ...
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bioRxiv - Plant Biology 2024Quote: ... The reaction mixtures were incubated for 1 h at room temperature and mixed with 4 μL of 6X gel loading dye (New England Biolabs). Following electrophoresis in 1.2% agarose gel containing SYBR safe DNA gel stain (Thermo Scientific ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 µg of DNA origami nanoparticle was mixed with 1 U/µl DNase I (2,000 units/mL, New England Biolabs, M0303S) mixed with 10× DNase I buffer diluted in water (Gibco) ...
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bioRxiv - Biochemistry 2024Quote: ... 1 μl of the PCR product was mixed with 1X KLD Reaction Buffer and 1X KLD Enzyme Mix (New England Biolabs). The reaction mix was then used to transform BL21 chemically competent cells ...
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bioRxiv - Biophysics 2024Quote: ... and labelled single-stranded oligo (50-fold excess relative to the DNA molarity) were mixed with either Nt.BbvCI (NEB, R0632) or Nb.BbvCI (NEB ...
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bioRxiv - Synthetic Biology 2024Quote: ... the appropriate linearized plasmid and purified DNA block was mixed into the NEBuilder HiFi DNA assembly master mix following standard protocol (NEB) and then transformed into dSL2 donor cells ...
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bioRxiv - Bioengineering 2024Quote: ... were mixed with 0.5 µL of NEBridge Golden Gate Assembly Kit (BsaI-HF v2) reaction mix (New England Biolabs E1601), 1 µL of T4 DNA ligase buffer ...
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bioRxiv - Cell Biology 2024Quote: ... and the digested fragments were mixed in a proportion of 3:1 in the presence of T4 DNA Ligase (New England Biolabs) to obtain the PP1α87B coding sequence in frame with N-terminal EGFP under the regulation of a metallothionein promoter (pMT-EGFP-PP1α87BWT) ...
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bioRxiv - Developmental Biology 2024Quote: ... 3PCR fragment and KpnI-NotI digested pattB plasmid was mixed and assembled by NEBuilder HiFi DNA Assembly Master Mix (NEB). From this reaction ...
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bioRxiv - Developmental Biology 2024Quote: PCR fragments and KpnI-BglII cut pattB vector was mixed and assembled using NEBuilder Hi Fi DNA Assembly Master Mix (NEB).
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bioRxiv - Cell Biology 2022Quote: ... 200 pmol of an equimolar mixture of all gene-specific oligos for each gene were mixed with 250 pmol of the appropriate FLAP oligo in 1x NEBuffer 3 (New England Biolabs, B7003), then incubated in a Thermocycler (BioRad ...
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bioRxiv - Genomics 2022Quote: ... Illumina libraries and DNA splint were mixed at a 1:1 ng ratio using NEBuilder HiFi DNA assembly Master mix (NEB #E2621). Any non-circularized DNA was digested overnight using ExoI ...
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bioRxiv - Developmental Biology 2021Quote: ... After annealing the complex an equimolar amount was mixed with 1000 ng Cas9 recombinant protein (NEB; final concentration 20 ng/μL) and incubated at RT for 15 min ...
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bioRxiv - Genomics 2023Quote: ... The gel-purified DGP-4 fragments (200 ng) and p200 vector (200 ng) were mixed and ligated in a 50 µL T4 ligation reaction (NEB, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The pellet was resuspended in 20 µl of distilled water and mixed with 18 µl gel loading dye (New England Biolabs, Inc.). Samples were resolved in a 10% SDS-polyacrylamide gel ...
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bioRxiv - Microbiology 2023Quote: ... antibiotic resistance cassette and 2 flanking regions were combined in equimolar amounts and mixed with 2 x HiFi reagent (NEB, UK) and incubated at 50°C for 1 hour ...
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bioRxiv - Genomics 2024Quote: ... 480 ng of the sgRNA-barcode library and 230 ng of the reporter construct were mixed in separate 20 µL NEBuilder HiFi assembly reactions (NEB, E2621S) for 1 h at 50 °C ...
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bioRxiv - Genomics 2024Quote: ... 50ng of LentiCRISPR v2 (addgen, #52961) and 5ng of PCR-amplified sgRNA pool was mixed with 1ul of BsmB1-V2 restriction enzyme (NEB; #R0580) and 1ul of T7 ligase (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... 50 ng of each sample was pooled together and mixed with 8 µL of NEBNext Quick Ligation Reaction Buffer (NEB, #B6058S), 6 µL of RNA adaptor (RMX ...
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bioRxiv - Genetics 2024Quote: ... Digested vector fragments and insert DNA fragments were mixed in a volume of 10 μl containing 2U T5 exonuclease (New England Biolabs, M0363S), 12.5U Phusion DNA Polymerase (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... 1.2 µl of linker oligonucleotide (Integrated DNA Technologies) were mixed with 2 µl 10x 5’DNA adenylation buffer (New England Biolabs, E2610L), 0.095 mM ATP ...
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bioRxiv - Synthetic Biology 2024Quote: ... Backbone and insert DNA were then mixed to the same final mass concentrations (≈15 ng/µL each) with 2x Gibson Assembly mix (New England Biolabs, E2611L) and incubated at 50 °C for 1 hour ...
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bioRxiv - Biophysics 2024Quote: ... the linearized backbone and insert were mixed in a 1:2 molar ratio and ligated using Gibson assembly (NEB, Cat. # E5510S) at 50°C for 15 min ...
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bioRxiv - Biochemistry 2024Quote: ... A 1 kb DNA ladder (New England Bio Labs #N3232S) was mixed with Gel Loading Dye Purple 6X (New England Biolabs #B7024S) and water ...
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bioRxiv - Immunology 2021Quote: ... 45 μl of cDNA from the synthesis reaction was mixed with primers and Q5® High-Fidelity 2X Master Mix(NEB, USA). The PCR program began with an initial denaturation at 95°C for 1.5minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 μl of the gRNA mixture was mixed with Cas9-NLS protein (final concentration of 5 μM. New England Biolabs, Ipswich, USA), 2M KCl (final concentration of 300mM) ...
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bioRxiv - Microbiology 2020Quote: ... RNA (25-100 ng) was mixed with random oligonucleotide primers using the NEBNext® First Strand Synthesis Module kit for Illumina® (NEB) and incubated for 10 min at 94°C ...
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bioRxiv - Genomics 2024Quote: The 15µL of purified DNA was mixed in a PCR reaction containing 1X NEBNext® High-Fidelity 2X PCR Master Mix (NEB #M0541L), 1 µM foward primer ...
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bioRxiv - Genomics 2020Quote: ... 50-100 fmol of PCR products were diluted in 9 µl then mixed with 1 µl of Rapid 1D Adapter and 1 µl of Ligase T4 Blunt (New England Biolabs, Ipswich, MA, USA). Pre-sequencing mix was incubated 10 min at 25°C and left on ice until ready to load ...
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bioRxiv - Biochemistry 2022Quote: ... and 0.01% Tween] to 3 µM concentration in a total volume of 50 µl and mixed with 20 μl of amylose beads (New England Biolabs, Ipswich, US-MA). After mixing the proteins and the beads ...
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bioRxiv - Biochemistry 2021Quote: ... 500 ng total of the purified PCR products were mixed with 1 μl 10× NEBuffer 2 (New England Biolabs Inc., Beverly, MA, USA) and ultrapure water to a final volume of 9.75 μl ...
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bioRxiv - Molecular Biology 2021Quote: ... The mixture containing RBBP6/CPSF/RBBP6+CPSF and MBP-MS2-bound L3 pre-mRNA was mixed with amylose beads (NEB, cat. No. E8021) equilibrated in pull-down buffer and incubated rotating at 4°C for 1.5 h ...
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bioRxiv - Genetics 2023Quote: ... 100 pmol of template oligos and 125 pmol IRDye 700-labeled reverse complement primer F1 were mixed in Phusion® High-Fidelity PCR Master Mix (NEB, Ipswich, MA). A 15s denaturing at 95°C following a 10-minute extension at 52°C afforded duplex DNAs ...
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bioRxiv - Bioengineering 2023Quote: ... The DNA fragments encoding enriched peptide genes were mixed with sfGFP gene portion and assembled by 1 hour incubation at 50°C using HiFi Master mix (NEB Japan, Tokyo, Japan). The reaction mixture was then used in PCR amplification with primers 13 and 14 to obtain peptide-sfGFP gene fragments suitable for cell-free protein synthesis ...
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bioRxiv - Developmental Biology 2023Quote: ... along with the guide RNA at a concentration of 300 ng/μl were mixed in molecular grade water with Cas9 buffer (NEB, Cat No. M0386S). Embryos were injected 6 hrs after egg laying for arm and 3hrs for frizzled4 and wnt7 ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μL annealed stem-loop oligonucleotide was mixed with 1 μL pre-linearized vector and ligated with T4 DNA ligase (cat. no. M0202, New England BioLabs, Inc., Ipswich, MA, USA), according to vendor’s ligation protocol ...
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bioRxiv - Genetics 2021Quote: ... Total RNA was mixed with Reaction Mix 1 (9:1 of 12 uM NEB RT random 6N-S to 12 uM oligo-dT(20), 12uM dNTPs ...
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bioRxiv - Biochemistry 2022Quote: ... purified KRAS protein was mixed with GMP-PNP (molar ratio of 10:1 GMP-PNP:KRAS) and calf intestinal alkaline phosphatase (NEB cat# M0290, 3 units per mg of KRAS). The reaction mixture was incubated for 3 hours at room temperature and then purified by size-exclusion chromatography on a 10/300 Superdex 75 GL column (GE Healthcare ...