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Citations for New England Biolabs :
451 - 500 of 1843 citations for Ethyl 7 oxo 7 2 thiomorpholinomethyl phenyl heptanoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... and 2 units of polymerase (New England Biolabs M0254). Vent exo– (New England Biolabs ...
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bioRxiv - Synthetic Biology 2024Quote: ... 25 µL of 2× Q5 Master Mix (NEB, UK) enzyme mix ...
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bioRxiv - Genetics 2024Quote: ... and 2 mM Ribonucleoside Vanadyl complex (New England Biolabs). Coverslips were preserved for later use in 70% EtOH at -20°C ...
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bioRxiv - Bioengineering 2024Quote: ... 2 μL of BsaI-HFv2 (NEB, 20000 U/mL), 2 μL of T4 DNA ligase (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... The digestion experiments used 2% AluI or MNase (NEB), 10% NEB CutSmart buffer or MNase buffer ...
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bioRxiv - Microbiology 2020Quote: ... amplified with the primers Dhm1275-1 and −2 (Table 2) using a NEBuilder HiFi DNA Assembly Kit (New England BioLabs, Ipswich, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... 5 μl of 100 ng/μl cDNA diluted from the previous step was combined with 2 μl block mix and 2 μl nuclease free water (NEB, catalog no. AM9937), then the cDNA-block oligo mix was incubated on a thermocycler under the following condition to allow block oligo mix to bind to 5’ and 3’ end of the cDNA molecule ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 µL ligation mix (2 µL 10x T4 ligase buffer, NEB, 1 µL T4 RNA ligase 2, truncated, NEB, 1 µL Ribolock inhibitor) was added and incubated (1 h ...
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bioRxiv - Evolutionary Biology 2024Quote: ... was prepared by combining 2 µL of 100 µM guide RNA (gRNA) with 2 µL of 20 µM EnGen SpyCas9 NLS (NEB, Cat. No. M0646T), and incubated at room temperature for 2-4 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 µL of linker ligation mixture was added (38% PEG-8000, 1x NEB T4 ligase buffer, 2 µM oBZ407_preA preadenylated linker, 100 units NEB T4 RNA ligase 2, truncated) and they were further incubated at 37 °C for 3 hrs ...
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bioRxiv - Microbiology 2020Quote: ... then quenched with 2 μL 6X purple loading dye (NEB) at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 µL of 10x uracil DNA glycosylase buffer (NEB M0280S), 2.5 µL RNase A (M0280S ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 units of 2’-O-Me-transferase (New England Biolabs) and 25 units RNasin Plus RNase inhibitor (Promega) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 200 U/uL of T4 RNA Ligase 2 (NEB M0351S), 1X T4 RNA Ligase Reaction Buffer (NEB B0216L) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 2 uL of 10X T4 RNA ligation buffer (NEB B0216L), 2 uL of 10mM ATP ...
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bioRxiv - Developmental Biology 2020Quote: ... a mix containing 2 μl of RNAse H (NEB, #M0297S), 1 μl of E ...
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bioRxiv - Genomics 2020Quote: ... 2 μL of NEB buffer 3.1(1X, New England Biolabs) and 1 μL of each DNA barcode B and ligation linker mix (B1-B50 ...
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bioRxiv - Microbiology 2022Quote: ... PEG 8000 (17.5% final) and T4 RNA ligase 2 (NEB) in 1x T4 RNA ligase buffer at 25°C for 1 h ...
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bioRxiv - Molecular Biology 2021Quote: ... and 0.5 units/µl T4 RNA Ligase 2 (NEB, M0239S), with the enzyme added after the other components had been mixed and incubated at 23°C for 5 min ...
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bioRxiv - Genomics 2020Quote: ... 2 μL of NEB DNA Quick Ligase (New England Biolabs) and 3 μL of Illumina Indexed adapter were added to the beads and incubated for 15 minutes at room temperature ...
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bioRxiv - Genomics 2020Quote: ... 12.5 μL NEBNext High-Fidelity 2× PCR Master Mix (NEB) were added and samples were PCR-amplified (72 °C 5 min ...
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bioRxiv - Genomics 2020Quote: ... 2× LongAmp Taq Master Mix (25 μl; New England Biolabs) was used to synthesize second-strand cDNA using 2 μl of the PR2 primer (ONT) ...
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bioRxiv - Genomics 2021Quote: ... 12.5 µL NEBNext High-Fidelity 2× PCR Master Mix (NEB) were added and samples were PCR-amplified (72 °C 5 min ...
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bioRxiv - Genomics 2021Quote: ... 12.5 µL NEBNext High-Fidelity 2× PCR Master Mix (NEB) were added to each well and samples were PCR-amplified (72 °C 5 min ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 µl T7 Endonuclease I (1 U/ul, M0302S, NEB) was added and incubated at 37°C for 1 hr ...
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bioRxiv - Genomics 2020Quote: ... 1 µl T4 RNA Ligase 2 truncated K227Q (200U; NEB)] was added ...
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bioRxiv - Genomics 2021Quote: ... 2 μL of 10x buffer 4 (New England Biolabs, NEB), 2 μL of 1 mg/ml bovine serum albumin solution (NEB) ...
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bioRxiv - Microbiology 2021Quote: F(ab’)2 fragments were generated using IdeZ protease (NEB), purified using CaptureSelect LC-lambda affinity matrix (human ...
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bioRxiv - Neuroscience 2020Quote: ... 12.5 µL NEBNext High-Fidelity 2× PCR Master Mix (NEB) were added and samples were PCR-amplified (72 °C 5 min ...
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bioRxiv - Microbiology 2020Quote: ... 2 U of HindIII (New England Biolabs, Beverly, MA, USA), 2 U of EcoRI (New England Biolabs ...
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bioRxiv - Systems Biology 2021Quote: ... PCR amplicons were purified from a 2% agarose gel (NEB), digested with EcoRI-HF and NotI-HF (NEB) ...
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bioRxiv - Genomics 2021Quote: ... 2 μL of 10x buffer 4 (New England Biolabs, NEB), 2 μL of 1 mg/ml bovine serum albumin solution (NEB) ...
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bioRxiv - Cell Biology 2021Quote: ... diluted 2-fold in 1x G3 buffer (New England Biolabs) and incubated with 160 U endoglycosidase H (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: ... and 2 μL T4 DNA Polymerase (#M0203S, New England Biolabs). The reaction was incubated at room temperature for 45 min with gentle mixing every 5 min ...
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bioRxiv - Microbiology 2021Quote: ... 2 µl of 20 µM SpCas9 (NEB, Cat. No. M0646M) was placed in the bottom of a 0.25 ml PCR tube ...
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bioRxiv - Neuroscience 2020Quote: ... 12.5 μL NEBNext High-Fidelity 2× PCR Master Mix (NEB) were added and samples were PCR-amplified (72 °C 5 min ...
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bioRxiv - Cell Biology 2021Quote: ... Clarified lysates were flowed over 2 mL amylose resin (NEB) equilibrated with MBP lysis buffer to capture MBP-tagged protein ...
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bioRxiv - Cell Biology 2021Quote: ... Then 2-5 unites of RNase H (New England Biolabs) in 5 µl of 1x RNase H buffer were added to the annealed RNA-chimeric oligo mixture and the RNase H cleavage reaction was performed at 37o C for 1 h ...
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bioRxiv - Molecular Biology 2022Quote: ... α2-3,6,8 neuraminidase (New England Biolabs, 25 U/μg protein) and α-N-acetyl-galactosaminidase (New England Biolabs ...
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bioRxiv - Synthetic Biology 2022Quote: ... 2 µl of T4 DNA Ligase Buffer (New England Biolabs), 1 µl of T4 or T7 DNA Ligase (New England Biolabs) ...
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bioRxiv - Synthetic Biology 2022Quote: ... 2 µl of T4 DNA Ligase Buffer (New England Biolabs), 1 µl of T4 or T7 DNA Ligase (New England Biolabs ...
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bioRxiv - Biophysics 2022Quote: ... 2 μL (1000 U) PNGase F (New England Biolabs Inc) were added and the reaction was incubated for 6 h at 37 °C ...
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bioRxiv - Biophysics 2022Quote: ... and 2 µM of SNAP-Surface Alexa Fluor 647 (NEB) in extracellular buffer for 30 minutes at 37 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... 100 units of 2′-O-Me-transferase (New England Biolabs) and 25 units RNasin Plus RNase inhibitor (Promega) ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 μl of 2.5 mM dNTP (New England Biolabs N0447S), 0.75 μl of 50 mM MgCl2 ...
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bioRxiv - Immunology 2022Quote: ... 2 mM ribonucleoside vanadyl complexes (New England Biolabs, Ipswich, MA), 0.02% ribonuclease-free bovine serum albumin (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2022Quote: ... 10x T4 DNA ligase buffer (2 ul; Cat # B0202S; NEB), Bsa I (1ul ...
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bioRxiv - Microbiology 2022Quote: ... 2 μl 10× T4 RNA ligase reaction buffer (NEB, M0242), 10 μl 50% PEG 8000 (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 μl T4 PNK (NEB, cat#M0201S, 10 U/μl), 1.5 μl T4 DNA Polymerase (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 μl T4 PNK (NEB, cat#M0201S, 10 U/μl) and 1.5 μl Klenow Fragment (3’->5’ exo- ...