Labshake search
Citations for New England Biolabs :
451 - 500 of 706 citations for Chocolate Agar with Bacitracin for Haemophilus isolation 15x100mm plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... Polyadenylated mRNA was purified from 50 ng of Total RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, #E7490). Library preparation was completed on purified mRNA using the NEBNext Ultra Directional RNA Library prep kit (NEB ...
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bioRxiv - Cancer Biology 2021Quote: ... chromatin from both IP and ‘Input’ samples were eluted and de-crosslinked at 65°C for 16 hours followed by DNA isolation by PCR and DNA cleanup kit (NEB). Enrichment of AR and MED1 at specified enhancers were quantified by qPCR using specific primers ...
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bioRxiv - Cell Biology 2021Quote: RNA used for polyA selected library generation for mRNA sequencing was isolated with the Monarch Total RNA isolation miniprep kit (New England Biolabs). Libraries were constructed using the Nugen Universal Plus mRNA-SEQ library construction kit (Nugen 0508 ...
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bioRxiv - Biochemistry 2021Quote: ... The NEBNext Ultra or Ultra II Directional RNA Library Prep Kit for Illumina with the mRNA magnetic isolation module (New England Biolabs) was used to prepare sequencing libraries from 1 μg of total RNA.
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bioRxiv - Cancer Biology 2020Quote: ... 200ng of total RNA with RIN >9.5 were used to prepare library using NEBNext poly(A) mRNA Magnetic Isolation Module and the NEB Next Ultra II RNA library prep kits for Illumina (New Engand Biolabs). Qubit High Sensitivity DNA kit (Life Technologies ...
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bioRxiv - Evolutionary Biology 2021Quote: ... mRNA libraries were constructed using the kits NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext UltraTM II Directional RNA Library Prep Kit for Illumina (New England BioLabs) following the manufacturer protocol ...
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bioRxiv - Bioengineering 2020Quote: ... and mRNA was isolated from ~1 μg of total RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490). RNA-seq libraries were constructed using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770 ...
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bioRxiv - Cell Biology 2021Quote: ... cells were lysed and total RNA was isolated with the Monarch RNA isolation kit (New England Biolabs, Ipswich, MA, USA), following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: Libraries for JU1373 and NIC58 were prepared simultaneously from mRNA isolated from 1 μg of pooled total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). RNA fragmentation ...
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bioRxiv - Immunology 2020Quote: ... 1μg RNA from each sample was used for mRNA enrichment using NEBNext Poly(A) mRNA magnetic isolation module (E7490S, NEB). RNA-seq libraries were constructed using the purified mRNA samples with NEBNext Ultra II Directional RNA library Prep Kit for Illumina (E7760S ...
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bioRxiv - Molecular Biology 2021Quote: ... EndoCβ-H1 and mouse islets was determined with an Agilent Bioanalyzer and mRNA enrichmed was performed from 500-1000ng of RNA using a NEBNext Poly(A) mRNA Magnetic Isolation Kit (NEB). Library preparation from mRNA and from miRISC-isolated RNA was performed using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Molecular Biology 2022Quote: RNA-seq libraries were prepared using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Samples were multiplexed using the NEBNext Multiplex Oligos for Illumina (NEB) ...
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bioRxiv - Cell Biology 2022Quote: ... Mature mRNA was enriched from 400 ng of total RNA per sample using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) and used to prepare libraries with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Bioengineering 2022Quote: ... and mRNA was isolated from ∼1 μg of total RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490). RNA-seq libraries were constructed using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770 ...
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bioRxiv - Immunology 2022Quote: ... Library construction (input: 40 ng per CD8+SP sample) was performed using a NEBNext Poly(A) Magnetic Isolation Module (#E7490; NEB) followed by a NEBNext Ultra II Directional RNA library prep kit for Illumina (#E7760 and/or #E7770 with #E7765 ...
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bioRxiv - Immunology 2022Quote: RNA from sorted Kmt2d-knockout and littermate control thymic cells was isolated by centrifugation to pellet and resuspended in 300 μL of Protection Buffer (Monarch RNA Isolation Kit, #T2010S; New England Biolabs Inc. (NEB), Ipswich ...
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bioRxiv - Systems Biology 2022Quote: Library preparation was performed with NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: RNA libraries were prepared using NEBNext Poly(A) mRNA Magnetic Isolation Module and the NEBNext Ultra II Directional RNA Library Prep Kit (NEB), according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... Strand-specific RNA libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina with NEBNEXT poly(A) mRNA Isolation Module (New England Biolabs) according to manufacturer recommendations ...
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bioRxiv - Molecular Biology 2022Quote: ... Poly(A)+ RNA transcript was isolated from 1 ug purified total RNA (RNA integrity number >9.0) with NEBNext poly(A) mRNA magnetic isolation module (New England Biolabs #E7490). RNA-seq libraries were prepared with NEBNext Ultra Directional RNA library preparation kit for Illumina (New England Biolabs #E7420S ...
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bioRxiv - Biochemistry 2022Quote: ... PolyA-selected libraries were made using NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra Directional RNA Library Prep Kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Zoology 2022Quote: ... where 250 nanograms of total RNA per tissue was used with the NEBNext Poly(A) Magnetic Isolation Module (New England BioLabs). Stranded cDNA libraries were created with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) ...
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bioRxiv - Physiology 2022Quote: ... RNA-seq libraries were generated with the NEBNext Ultra II Directional RNA Library Prep Kit coupled with Poly(A) mRNA Magnetic Isolation Module and NEBNext multiplex oligos for Illumina (New England Biolabs). Deep sequencing was performed on the NextSeq 500 sequencing system (Illumina ...
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bioRxiv - Developmental Biology 2022Quote: ... Barcoded stranded mRNA-seq libraries were prepared from 300 ng of high-quality total RNA samples using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Neuroscience 2024Quote: ... using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Cell Biology 2024Quote: ... Libraries were generated from 250 ng of total RNA as following: mRNA enrichment was performed using the NEBNext Poly(A) Magnetic Isolation Module (New England BioLabs). cDNA synthesis was achieved with the NEBNext RNA First Strand Synthesis and NEBNext Ultra Directional RNA Second Strand Synthesis Modules (New England BioLabs) ...
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bioRxiv - Cancer Biology 2024Quote: ... 1ug of DNase-treated RNA was then used for NEB Next Poly(A) mRNA isolation and subsequent next generation sequencing library construction (NEB) following factory procedures ...
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bioRxiv - Systems Biology 2024Quote: Approximately 100 ng of each RNA sample was used to create Illumina sequencing libraries using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina with NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µg of total RNA was used for standard library preparation using NEBNext PolyA mRNA magnetic isolation module (NEB, E7490). In brief ...
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bioRxiv - Developmental Biology 2024Quote: ... mRNA Magnetic Isolation Module (E7490L) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760L, New England Biolabs (NEB), Ipswich ...
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bioRxiv - Immunology 2023Quote: ... PolyA mRNA was isolated using the NEBNext® Poly(A) mRNA magnetic isolation module (E7490, New England Biolabs, Ipswich, MA). RNA-seq libraries were obtained using the NEB-Next® UltraTM II RNA library prep kit for Illumina® (E7770S ...
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bioRxiv - Molecular Biology 2023Quote: The phenol chloroform extracted RNA from previous step weas further purified to select for polyadenylated RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) and reverse transcribed using SuperScript III reverse transcriptase (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: Verification of modification of the pv6 locus was performed by first isolating genomic DNA from transfected parasites (Monarch genomic DNA isolation kit, New England Biolabs). Integration of targeting plasmid at the pfs47 locus was determined with primer pairs CVO119-CVO120 (wildtype locus and integrated locus) ...
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bioRxiv - Plant Biology 2023Quote: ... Total RNA of 500 ng was used to enrich the mRNA using NEBNext Poly (A) mRNA magnetic isolation module (Catalog: E7490, New England Biolabs) by following the manufacturers’ protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Library preparation and selection of poly-adenylated transcripts were done using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, E7490) and xGen Broad-range RNA Library Prep (IDT ...
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bioRxiv - Genomics 2023Quote: Directional poly(A)+ RNA-Seq libraries were prepared using 300 ng of DNase-treated RNA using the Poly (A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep kit for Illumina (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA libraries were generated using 1µg RNA with the magnetic mRNA isolation module and NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs). DNA library was amplified by PCR and quality was analyzed using the fragment analyzer (Advanced Analytical) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... RNA library preparation was performed on total or polysomal RNA using a NEBNext Poly(A) mRNA Magnetic Isolation Module and a NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) according to the manufacturer’s instructions with 1 μg of RNA as a starting point ...
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bioRxiv - Cancer Biology 2023Quote: RNA-Seq libraries were generated from 250 ng of total RNA as following: mRNA enrichment was performed using the NEBNext Poly(A) Magnetic Isolation Module (New England BioLabs). cDNA synthesis was achieved with the NEBNext RNA First Strand Synthesis and NEBNext Ultra Directional RNA Second Strand Synthesis Modules (New England BioLabs) ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... with prior enrichment of mRNA by using Oligo dT beads from the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB). After library preparation ...
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bioRxiv - Cell Biology 2023Quote: Poly(A)+ RNA was isolated from nuclei and whole cells (see C2C12 cell fractionation above) using the Magnetic mRNA Isolation Kit (New England Biolabs). Two rounds of binding ...
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bioRxiv - Genomics 2023Quote: ... 100 ng of total RNA was used as input for mRNA selection and conversion to cDNA using the NEBNext Poly(A) Magnetic Isolation Module (New England BioLabs). Sequencing libraries were prepared using the NEBNext Ultra II RNA Library Preparation Kit for Illumina (New England BioLabs) ...
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bioRxiv - Physiology 2023Quote: ... Libraries were generated from 250 ng of total RNA as following: mRNA enrichment was performed using the NEBNext Poly(A) Magnetic Isolation Module (New England BioLabs). cDNA synthesis was achieved with the NEBNext RNA First Strand Synthesis and NEBNext Ultra Directional RNA Second Strand Synthesis Modules (New England BioLabs) ...
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bioRxiv - Plant Biology 2023Quote: ... We used 1 μg total RNA for mRNA purification using the NEBNext Oligo d (T) 25 magnetic isolation module (New England Biolabs), followed by first strand cDNA synthesis using NEBNext Ultra II RNA Library Prep Kit for Illumina according to the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2023Quote: ... Then enrichment of Poly(A) mRNA was performed with the ‘NEBNext Poly(A) mRNA Magnetic Isolation Module’ (New England Biolabs). RNA libraries were prepared using the ‘NEBNext Ultra Directional RNA Library Prep Kit for Illumina’ (New England Biolabs) ...
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bioRxiv - Developmental Biology 2023Quote: ... 100 ng of total intact RNA was used with the NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEB, #E7490S), and the enriched mRNA was prepped for sequencing with the NEBNext® Ultra™ II Directional RNA Library Prep with Sample Purification Beads (NEB ...
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bioRxiv - Genomics 2023Quote: ... Library preparation has been done with 250ng of input RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) followed by NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760 ...
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bioRxiv - Genomics 2023Quote: ... and the resulting libraries prepared for Illumina sequencing using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs) and the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... brucei BFs CN PIP5Pase exclusively expressing V5-tagged PIP5Pase WT or mutant D360A/N362A for 24 h using the magnetic mRNA isolation kit (New England Biolabs) according to manufacturer’s instructions ...
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Vps18 contributes to phagosome membrane integrity in Mycobacterium tuberculosis-infected macrophagesbioRxiv - Microbiology 2023Quote: ... containing mRNA was isolated from the RNA extracted from the flow sorted samples using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). cDNA libraries were constructed using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina ...