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Citations for New England Biolabs :
451 - 500 of 2735 citations for Acyl protein thioesterase 2 LYPLA2 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were transferred to nitrocellulose membranes (Biolabs, 1620115). Membranes were then incubated with primary antibody diluted in 5% milk or 2.5% BSA in Triton X-100-TBS buffer (T-TBS ...
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bioRxiv - Developmental Biology 2021Quote: ... we injected Cas9 mRNA or protein (NEB, M0646T) with corresponding sgRNAs into 1-cell wild-type Tuebingen embryos ...
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bioRxiv - Developmental Biology 2021Quote: Protein extraction was performed with RIPA buffer (NEB) in the presence of protease and phosphatase inhibitors (Sigma ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins with or without PNGase F (NEB, USA) digestion were mixed with the loading buffer (250Mm Tris-HCl PH 6.8 ...
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bioRxiv - Molecular Biology 2020Quote: ... 90 nM of SpCas9 protein (New England Biolabs), Cas9 nuclease buffer (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: ... PNGase F (P0704, NEB, 1,000 units/µg protein) was used to remove the N-linked glycosylation in purified ORF8 protein ...
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bioRxiv - Cell Biology 2021Quote: ... samples were treated with Lambda Protein Phosphatase (NEB) for 30 min at 30 °C followed with an additional four times washing step before they were blocked ...
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bioRxiv - Microbiology 2020Quote: ... and proteins were purified with amylose resin (NEB) and eluted with 20 and 50 mM maltose (Sigma).
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bioRxiv - Plant Biology 2021Quote: ... All proteins were purified using Amylose Resin (NEB) or HisPur Cobalt Resin (Thermo ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... 1:8 dilution Cas9 protein (New England Biolabs, cat ...
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bioRxiv - Cancer Biology 2022Quote: ... 10µl of Protein G magnetic beads (NEB, S1430S) was used ...
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bioRxiv - Molecular Biology 2022Quote: ... 1μl of T4 bacteriophage protein (NEB, 5ng/μl), 3μl of DMSO ...
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bioRxiv - Molecular Biology 2019Quote: ... The protein was purified using amylose resin (NEB) using the provided protocol ...
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bioRxiv - Pathology 2020Quote: ... plasma and histone H3 protein (New England Biolabs) as standard were subjected to SDS-PAGE and electrically transferred onto PVDF membrane (Millipore) ...
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bioRxiv - Biophysics 2020Quote: ... Eluted protein was deglycosylated with PNGase F (NEB) by incubating 5 units of the enzyme per 1 μg of protein for 2 h at 37 °C under gentle agitation ...
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bioRxiv - Developmental Biology 2020Quote: ... The gRNAs were mixed with protein Cas9 (NEB), allowed 5 minutes at 37°C to assemble into ribonucleoprotein complexes ...
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bioRxiv - Microbiology 2019Quote: ... Lactis Protein Expression kit (New England Biolabs, UK) was used with the pKLAC2 vector ...
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bioRxiv - Cell Biology 2020Quote: ... 10 μL Protein G magnetic beads (NEB, S1430S) were used ...
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bioRxiv - Cell Biology 2019Quote: ... The gRNA and Cas9 protein (New England Biolabs) were injected into one-cell stage NHGRI-1 embryos in a 2 nl volume consisting of ∼50 ng/μl gRNA and ∼300 nM Cas9 ...
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bioRxiv - Cell Biology 2022Quote: ... were added to 1x protein kinase buffer (NEB) supplemented with 200 μM “cold” ATP (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... Ribonucleoprotein complexes of SpCas9 protein (NEB; Ipswitch, MA), ssODN template and gRNA were prepared with final concentration 200 μg/μl ...
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bioRxiv - Synthetic Biology 2022Quote: ... and Broad Range Protein Marker (New England Biolabs).
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bioRxiv - Microbiology 2023Quote: ... coli protein synthesis system (New England BioLabs inc.). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... 400 units mL-1 lambda protein phosphatase (NEB), and 0.1 μg mL-1 protein phosphatase 2a (Cayman Chemical ...
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bioRxiv - Cell Biology 2023Quote: ... and PMP with Lambda Protein Phosphatase (P0753S, NEB) with Phosphatase inhibitor cocktail (4X ...
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bioRxiv - Molecular Biology 2023Quote: ... The proteins were purified by Amylose resin (NEB) and eluted with 10 mM maltose ...
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bioRxiv - Biophysics 2023Quote: ... and phosphorylated with protein kinase A (PKA) (NEB) for 1 hour at 20 °C ...
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bioRxiv - Biochemistry 2024Quote: ... 30nM sgRNA and 30 nM Cas9 protein (NEB) for 1h at room temperature and analyzing cleavage products on a 2% agarose gel ...
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The RNA-binding protein RbpB is a central regulator of polysaccharide utilization in gut BacteroidesbioRxiv - Microbiology 2023Quote: ... coli protein synthesis system (PURExpress, New England Biolabs). Reactions were performed according to manufacturer’s instruction and as previously described in 82 with a few modifications ...
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bioRxiv - Plant Biology 2019Quote: ... 2 µg total RNA was incubated at 37°C for 30 min together with DNaseI (2 U, NEB). To inactivate the DNaseI ...
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bioRxiv - Immunology 2021Quote: ... the samples were added to 2 μL of second-strand synthesis mix containing 2.25× NEB buffer 2 (NEB), 0.625mM each dNTP Mixture (NEB) ...
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bioRxiv - Genomics 2023Quote: ... The purified products were subjected to dA tailing with 2 μL of 10X NEBuffer 2 (New England BioLabs), 0.1 μL of 100 mM dATP ...
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bioRxiv - Genomics 2023Quote: ... 2 εL of 10% Tween-20 and 5 εL NEBNext High-fidelity 2× PCR Master Mix (NEB, M0541) was added to each well sequentially ...
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bioRxiv - Microbiology 2020Quote: ... Cloning and purification of full length IE86 protein: Full length IE86 protein was codon optimized and cloned in pMALcx5 vector (New England Biolabs Inc.). Cells were transformed in BL-21 cells and IE86-FL-pMALcx5 transformed cells were grown in 1L luria broth (Thermofisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... Phosphorylation of purified LPR1 protein was tested according to (Maldonado-Bonilla et al., 2014) using λ-protein phosphatase (New England Biolabs). In brief ...
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bioRxiv - Genetics 2022Quote: ... along with 6uL of EZ-Run Pre-Stained Rec Protein Ladder (Fisher BP3603-500) and 3uL of Blue Prestained Protein Standard (NEB P7718S). Gels were electrophoresed in XT Tricine Running Buffer (Bio-Rad 1610790 ...
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bioRxiv - Synthetic Biology 2023Quote: The cell-free protein synthesis (CFPS) reactions were carried out using the PURExpress In Vitro Protein Synthesis Kit (New England Biolabs Inc) in 384-well plates (Thermofisher NUNC ...
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bioRxiv - Microbiology 2023Quote: RdnE proteins were produced using the New England Biolabs PURExpress In Vitro Protein Synthesis Kit (New England BioLabs Inc., Ipswich MA). Template DNA contained the rdnE gene and required elements specified by the PURExpress kit ...
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bioRxiv - Cell Biology 2023Quote: ... a PCR fragment containing the complete Nv-osk coding sequence was synthesized for protein expression with PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs) using manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... To remove any remaining phosphorylation the GFP-Trap bound proteins were treated with 40 units of Lambda Protein Phosphatase (New England Biolabs, P0753S) at 30°C for 30min ...
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bioRxiv - Immunology 2021Quote: ... Libraries were prepared using the NEBNext Immune Sequencing Kit for Human (New England Biolabs) according to the manufacturer’s instructions without modifications ...
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bioRxiv - Genomics 2020Quote: ... the circularized DNA was initially treated with human alkyl adenine DNA glycosylase (hAAG, NEB) to cleave the N-glycosidic bond of etheno-dA bases ...
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bioRxiv - Cell Biology 2020Quote: ... 2 µl 10 mM ATP (NEB, 0756), 1 µl 100 mM DTT (Cell Signaling Technology Europe ...
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bioRxiv - Cell Biology 2020Quote: ... in 1x glycobuffer 2 (New England Biolabs), and 10% NP-40 (New England Biolabs ...
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bioRxiv - Genomics 2020Quote: ... and 2 μL T4 PNK (NEB, M0201L) were added to the sample in respective order ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 2 µl of 20 µM SpCas9 (NEB, Cat ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 3.13 μL BSA 2 mg/mL (NEB), 12.5 μL FastDigest NotI (ThermoFisher) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 3.13 μL BSA 2 mg/mL (NEB), 12.5 μL FastDigest NotI (ThermoFisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... 10ng of 2-log ladder (NEB N3200L) was loaded instead of 100ng ...
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bioRxiv - Genomics 2020Quote: ... in 1X Buffer 2 (New England Biolabs) at 37°C for 1 hr ...