Labshake search
Citations for Illumina :
601 - 650 of 1399 citations for rno mir 32 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... The best replicate from each tissue was selected for the transcriptome data set by preparing TrueSeq libraries (Illumina, San Diego, CA, USA). The rest of the samples were prepared with the Lexogen QuantSeq 3’mRNA kit (Lexogen ...
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bioRxiv - Genomics 2022Quote: ... all of the ChIP DNA and 220 ng of input DNA were mixed with 3 units of T4 DNA polymerase (NEBNext® DNA Library Prep Master Mix Set for Illumina, #E6040L) to create blunt ends ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were constructed from 800 ng total RNA (NEBNext Multiplex Small RNA library prep set for Illumina, New England Biolabs, NEB-E7560S) and the small RNA fraction was sequenced on the NextSeq 500 System (Illumina ...
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bioRxiv - Neuroscience 2023Quote: ... libraries were constructed from 1000 ng total RNA (NEBNext Multiplex Small RNA library prep set for Illumina, NEB-E7560S, New England Biolabs) and the small RNA fraction sequenced (NextSeq 500/550 High Output Kit v2.5 75 Cycles ...
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bioRxiv - Microbiology 2021Quote: ... 5μL of Nextera XT Index 2 Primers (S5XX) (both from the Nextera XT Index kit, Illumina, USA), 10μL PCR grade water and 25 μL of KAPA© HiFi Hot Start ReadyMix (Roche ...
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bioRxiv - Microbiology 2019Quote: ... for 2 hours at 25 °C and reverse transcription was performed with Reverse Transcription Primer (Illumina sequence) using SuperScript IV Reverse Transcriptase (ThermoFisher ...
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bioRxiv - Microbiology 2020Quote: ... Amplicons generated using the modified primers were barcoded using Nextera XT index kit (Illumina, San Diego, CA). An Illumina MiSeq instrument was used for sequencing 250 base pairs in both directions ...
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bioRxiv - Microbiology 2022Quote: ... and samples were labelled with index primers using Nextera XT Index kit (Illumina, San Diego, CA, USA). Paired end sequencing was performed with the Illumina MiSeq system ...
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bioRxiv - Genomics 2023Quote: ... Sequencing with standard Illumina primers was performed on a NextSeq 500/550 instrument (Illumina, San Diego, USA) with a read length of 150 + 150 bp (paired-end).
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bioRxiv - Cell Biology 2023Quote: ... and 20 μM reverse P7 primers with 6-bp TruSeq indices that are automatically demultiplexed by Illumina software ...
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bioRxiv - Plant Biology 2021Quote: Quantitative PCR (qPCR) was carried out on an Illumina Eco Real-Time PCR platform (Illumina, Melbourne, Australia), following the protocol outlined by Barrett-Manako and colleagues (Barrett-Manako et al. ...
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bioRxiv - Genomics 2020Quote: ... PCR-free sequencing libraries were generated using Illumina TruSeq DNA PCR-Free Library Preparation Kit (Illumina, USA) following the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2023Quote: PCR-free sequencing libraries were generated using the Illumina TruSeq DNA PCR-Free Library Preparation Kit (Illumina) following the manufacturer’s recommendations ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCR-free sequencing libraries were generated using the Illumina TruSeq DNA PCR-Free Library Preparation Kit (Illumina) following the manufacturer’s recommendations ...
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bioRxiv - Systems Biology 2019Quote: ... six-base indexes distinguish samples and allow multiplexed sequencing and analysis using 48 unique indexes ((Set A: indexes 1-12 (Illumina, RS-200-0012), Set B ...
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bioRxiv - Genomics 2020Quote: ... Libraries were indexed and pooled into 12 sample sets and sequenced as 101 bp paired-end reads using Illumina HiSeq2500 (Illumina, San Diego, CA). Raw data have been submitted to GEO (http://ncbi.nlm.nih.gov/geo ...
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bioRxiv - Microbiology 2022Quote: ... region was amplified using the primer set (16S-341F/805R, see Table S2 for nucleotide sequences) according to the 16S Metagenomic Sequencing Library Preparation procedure (Illumina; San Diego, CA). The success of PCR amplification was confirmed using agarose electrophoresis (1.5%) ...
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bioRxiv - Cancer Biology 2023Quote: ... and sequencing error probability 0 ≤ ϵ ≤ 1 (which we set to .01 in our experiments to proxy the per-base sequencing error rate in Illumina sequencing instruments), we can enumerate the likelihood of drawing the observed reads from all possible underlying allele statuses ...
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bioRxiv - Cell Biology 2020Quote: ... Lentiviral sgRNA inserts were amplified in a two-step PCR (with Illumina adapters added on the second PCR), as previously described39 ...
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bioRxiv - Genetics 2021Quote: ... and a unique combination of the dual barcoded primers P5 and P7 Nextera XT Index kit (Illumina #15055293) following the cycling conditions ...
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bioRxiv - Plant Biology 2022Quote: ... The library from each genotype was indexed using Unique Dual Index Primer Pairs (NEBNext Multiplex Oligos for Illumina) in order to perform multiplexed sequencing.
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bioRxiv - Plant Biology 2020Quote: ... custom adapters for selecting 5’-P mRNAs and primers from the TruSeq Small RNA Sample Preparation Kit (Illumina) for multiplexing the libraries as indicated in Zhai et al (2014) ...
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bioRxiv - Microbiology 2021Quote: ... each 1 μL of 10 μM forward and reverse primers from Nextera XT Index Kit v2 (Illumina, USA), 8 μL of nuclease-free water ...
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bioRxiv - Synthetic Biology 2022Quote: ... and a unique combination of the dual-barcoded primers P5 and P7 Nextera XT Index kit (Illumina #15055293). The cycling conditions were ...
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bioRxiv - Genomics 2022Quote: ... 2.5 μL each of Nextera i5 and i7 indexed amplification primers (Nextera Index Kit, Illumina, FC-121—1011) and 10 μl of nuclease free H2O ...
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bioRxiv - Genetics 2024Quote: ... Primers were designed to amplify barcoded donor sequences on the plasmid pools to quantify strain abundance by Illumina sequencing with 2 × 150 bp reads (Novogene).
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bioRxiv - Evolutionary Biology 2021Quote: ... 100bp TruSeq DNA PCR-free libraries (Illumina) were prepared according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... an Eco Real-Time PCR System (Illumina) or a 7500 Real Time PCR System (Applied Biosystems).
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bioRxiv - Genomics 2022Quote: ... TruSeq DNA PCR free (Illumina Inc., USA) and Collibri PCR free PS DNA (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... 25 μl PCR master mix (Nextera, Illumina) and 5 μl indexed amplification primers59 (0.125 μM final concentration ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... or Eco Real-Time PCR System (Illumina) using SuperScript One Step RT-PCR kit (Life Technology) ...
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bioRxiv - Genomics 2023Quote: ... 100bp TruSeq DNA PCR-free libraries (Illumina) were prepared according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... The library was made according to the manufacturer’s directions for the TruSeq Stranded mRNA LT Sample Prep Kit - set B (Illumina, Cat. No. RS-122-2102). The resulting short fragment library was checked for quality and quantity using the LabChip GX (PerkinElmer ...
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bioRxiv - Immunology 2021Quote: Library preparation for sequencing was performed using 200 ngs of total RNA input with the TrueSeq RNA Sample Prep Kit v2-Set B (RS-122-2002, Illumina Inc, San Diego, CA) producing a 275bp Fragment including Adapters in average size ...
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bioRxiv - Microbiology 2022Quote: Short read sequencing libraries were prepared using the Illumina DNA Prep kit in combination with IDT® UD Indexes Set A (Illumina, USA, #20018705, #20027213). Between 66.3 ng and 200 ng per sample was used as starting material ...
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bioRxiv - Microbiology 2022Quote: ... the 16S and ITS amplicon PCRs were subjected to amplification with IDT for Illumina Nextera DNA Unique Dsual Indexes (Set A, now called IDT for Illumina DNA/RNA UD Indexes) (produced by ...
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bioRxiv - Systems Biology 2022Quote: The sequencing library preparation was done using 200 ng of total RNA input with the TrueSeq RNA Sample Prep Kit v3-Set B (RS-122-2002, Illumina Inc, San Diego, CA) producing a 275 bp fragment including adapters in average size ...
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bioRxiv - Synthetic Biology 2021Quote: ... Sequencing libraries were generated with purified PCR products using Illumina TruSeq DNA PCR-Free Library Preparation Kit (Illumina, USA) following manufacturer’s recommendations and index codes were added ...
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Legionella relative abundance in shower hose biofilms is associated with specific microbiome membersbioRxiv - Microbiology 2023Quote: ... The second PCR (adaptor PCR) was performed with limited cycles to attach specific sequencing Nextera v2 Index adapter (Illumina). After purification ...
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Engineering TALE-linked deaminases to facilitate precision adenine base editing in mitochondrial DNAbioRxiv - Molecular Biology 2023Quote: ... The final PCR products were purified with a PCR purification kit (MGmed) and sequenced using a MiniSeq sequencer (Illumina). Base editing frequencies from targeted deep sequencing data were measured using source code (https://github.com/ibs-cge2/prime_editor_analysis ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... pre-amplified samples were used for TaqMan quantitative PCR which was performed on Eco Real-Time PCR System (Illumina) using gene specific primers purchased from Fluidigm (DELTAgene Assay ...
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bioRxiv - Microbiology 2024Quote: ... The second PCR (adaptor PCR) was performed with limited cycles to attach specific sequencing Nextera v2 Index adapter (Illumina). After purification ...
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bioRxiv - Microbiology 2020Quote: ... 20 ng of environmental DNA were used as template for amplification of 16S rRNA gene using V3-V4 primers (341F and 785R) as previously reported.25,26 Libraries were constructed and sequenced on a MiSeq apparatus27 (Illumina) by BMR Genomics (Padua ...
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bioRxiv - Genomics 2020Quote: ... 0.5 μl of each index primers i5 and i7 from Nextera Index Kit (Illumina, catalog no. FC-121-1011) and 1.5 μl H2O ...
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bioRxiv - Genomics 2020Quote: ... 0.5 μl of each index primers i5 and i7 from Nextera Index Kit (Illumina, catalog no. FC-121-1011) and 1.5 μl H2O ...
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bioRxiv - Microbiology 2019Quote: ... 2010) primers and sequencing was conducted on an Illumina MiSeq using a MiSeq Reagent Kit v3 (600 cycles; Illumina) and 30% PhiX Control v3 (Illumina).
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bioRxiv - Immunology 2021Quote: ... Sequencing libraries were constructed using 200 pg cDNA as input for the NexteraXT kit with NexteraXT indexing primers (Illumina). Libraries were quality assessed ...
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bioRxiv - Genomics 2022Quote: ... Enrichment was carried out with indexed-primers according to an adapted protocol from Multiplexing Sample Preparation Oligonucleotide kit (Illumina). We polled the enriched libraries in equi-molarity and sequenced them on an Illumina HiSeq 2500 sequencing instrument at GIS to generate 2×101 base-pair reads ...
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bioRxiv - Genomics 2021Quote: ... was performed on Illumina Hiseq-2500 or NovaSeq 6000 system using custom sequencing primers according to manufacturer’s instructions (Illumina). WGS was carried out on 263 diverse SSD-PGR from the T3Cs plus 191 diverse genotypes from the cultivar panel (Supplementary Note) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The libraries were amplified and both the indexed and universal primer (NEBNext Multiplex Oligos for Illumina, New England Biolabs) were added by PCR using HGS Diamond Taq DNA polymerase (Eurogentec) ...