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Citations for Illumina :
151 - 200 of 1392 citations for rno mir 155 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... qRT-PCR was conducted using AceQ Universal SYBR qPCR Master Mix (Vazyme, Nanjing, China) on an RT-PCR system (Illumina Eco, California, USA). The primers used are listed in Table 1 ...
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bioRxiv - Cell Biology 2020Quote: ... RT-qPCR was performed in triplicate with the Eco Real-Time PCR System (Illumina, CA, USA), using primer sets for (Supplementary Table 1) ...
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bioRxiv - Microbiology 2024Quote: ... V3–V4 region of the 16S rRNA gene were amplified using the primer set 341F– 785R (Klindworth et al. 2013) and sequenced using Illumina MiSeq 2 x 300 bp (Illumina Inc., San Diego, CA, United States). Demultiplexed and adapter clipped reads obtained from LGC Genomics were processed as mixed ones ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR amplicons were purified by gel extraction and the concentrations of each sample was adjusted to 25 ng/µl for the 2nd stage barcoding PCR using TG Nextera XT Index Kit v2 Set A (Illumina) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Samples were then indexed by ligating index barcode to Illumina adapters onto the PCR amplicon using the Nextera XT Index Kit v2 Set A (Illumina). Indexed amplicon DNA concentrations in each sample were quantified ...
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bioRxiv - Genomics 2021Quote: ... 0.2 mM of primers (PE1.0 and Illumina multiplex primer), for 8—16 cycles depending on library strength ...
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bioRxiv - Zoology 2021Quote: ... Subsequently a second PCR was performed to apply the Illumina sequencing adapters Nextera XT Index Primer (Illumina) (69) ...
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bioRxiv - Bioengineering 2024Quote: ... A second PCR was performed with indexing primers to attach unique dual identifiers (Illumina, San Diego, CA) to the amplicons ...
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bioRxiv - Genomics 2023Quote: ... and 1.25µM indexing primers (Ad1_noMX primer and Ad2.x indexing primer 45 or IDT for Illumina dual index primers (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... Set D (Illumina, San Diego, USA). The sequences of the four sets of primers used during library preparation (limited cycle PCR ...
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bioRxiv - Genomics 2019Quote: ... Probe sets (ILLUMINA HumanHT 12 V4) were assigned to gene names ...
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bioRxiv - Cancer Biology 2022Quote: ... Set A (Illumina, RS-122-2001) was used to generate RNA-seq libraries according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Set A (Illumina #RS-122-2301) according to manufacturer’s recommendation ...
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bioRxiv - Cancer Biology 2024Quote: ... and UD Indexes Set A (Illumina). Ten cycles of PCR amplification were applied to all libraries.
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bioRxiv - Microbiology 2022Quote: ... the index PCR step was performed as in the protocol except using IDT for Illumina DNA/RNA UD Indexes Set A (Illumina, 20027213), MyFi Mix (BIOLINE ...
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bioRxiv - Genomics 2020Quote: ... In a second round of PCR (PCR2) each sample was barcoded with unique Truseq DNA Index primers (Illumina). DNA concentration was measured by fluorometric quantification (Qubit ...
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bioRxiv - Microbiology 2021Quote: ... a second PCR was used to apply dual indices and Illumina sequencing adapters Nextera XT Index Primer (Illumina), by 7 cycles PCR (16S Metagenomic Sequencing Library Preparation ...
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bioRxiv - Microbiology 2021Quote: ... Eluted DNA was amplified by PCR using NGS primers supplied with the kit (NEBNext Multiplex Oligos for Illumina, Index Primers Set 1 and 2 ...
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bioRxiv - Microbiology 2022Quote: The second round of PCR was performed with Illumina Nextera XT indexing primers (Illumina Inc., San Diego, CA) to generate amplicons compatible with Illumina MiSeq sequencing chemistry ...
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bioRxiv - Cancer Biology 2024Quote: ... and PCR-amplified using customize primers (Supplementary Material) designed following Illumina Adapter Sequences manual (Illumina, Document #1000000002694 v16) instructions ...
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bioRxiv - Cell Biology 2024Quote: ... sgRNA inserts were PCR amplified (primers listed in Supplementary Table 5, purified and sequenced on a NextSeq (Illumina). Sequencing reads were mapped and the abundance of each sgRNA was tallied ...
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bioRxiv - Cell Biology 2019Quote: ... Indexing primers (Illumina, FC-131-2001 ...
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bioRxiv - Pathology 2019Quote: ... Amplicons were then indexed using the Nextera XT Index Kit V2 set A and set D (Illumina) and purified again with AMPure XP beads to remove low molecular weight primers and primer-dimer sequences ...
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bioRxiv - Microbiology 2021Quote: Indexing of purified PCR products was performed using the Nextera XT Index kit v2 (Set D) as per manufacturer’s instruction (Illumina Inc, CA, USA). The libraries after indexing were quantified using Qubit dsDNA BR assay kit (Invitrogen ...
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bioRxiv - Genetics 2021Quote: Purified samples were then amplified by PCR (maximum 12 cycles) using barcoded primers from the Nextera Index Kit (Illumina). To determine the optimal number of cycles for each sample ...
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bioRxiv - Genetics 2024Quote: ... according to the manufacturer’s instructions at a final primer concentration of 250 nM in Eco Real-Time PCR System (Illumina) under the following conditions ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR was performed using unique dual index primer pairs (NEBNext Multiplex Oligos for Illumina from New England BioLabs, # E6440S) according the following parameters ...
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bioRxiv - Genomics 2020Quote: ... Set A (Illumina catalog # FC-122-1001) in accordance with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Small RNA Library Prep Set from Illumina (New England BioLabs Inc. ...
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bioRxiv - Genomics 2019Quote: ... Set A (Illumina catalog # FC-122-1001) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... QHR-4C libraries were constructed with specific primer pairs (forward primers containing Illumina P5 with sequences near a specific viewpoint and reverse primers containing Illumina P7 with an index and sequences matching the adapter ...
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bioRxiv - Plant Biology 2019Quote: ... RT-qPCR was performed as described previously (Nakamichi et al., 2010) using an Eco Real-Time PCR System (Illumina). Gene expression was normalized against IPP2 ...
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bioRxiv - Cancer Biology 2019Quote: ... and barcoded reverse primers for each sample (NEBNext Index primers for Illumina). PCR products were purified using Qiagen Qiaquick PCR purification kit ...
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bioRxiv - Cell Biology 2021Quote: ... and barcoded reverse primers for each sample (NEBNext Index primers for Illumina). PCR products were purified using 1.8 volumes of Axygen AxyPrep magnetic beads (MAG-PCR-CL) ...
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bioRxiv - Evolutionary Biology 2020Quote: We performed a second round of PCR to attach i5 and i7 Illumina indices (Nextera XT Index Kit v2 set A; Illumina, San Diego, California) to the PCR amplicons ...
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bioRxiv - Microbiology 2021Quote: ... Next the amplicons were checked for their size and purity on a 1.5 % agarose gel and if suitable subjected to the index PCR using the Nextera XT Index Kit v2 Set C/D (Illumina, FC-131-2003). After index PCR the samples were cleaned again with AmPure XP Beads (Beckman Coulter Life Science Cat ...
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bioRxiv - Genomics 2020Quote: ... We performed adapter ligation using Illumina TruSeq Single DNA Indexes Set A and Set B (Illumina, San Diego, USA), and performed adaptor enrichment using (8 ...
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bioRxiv - Immunology 2023Quote: ... NexteraXT V2 primers (Illumina) were used to allow larger scale multiplexing ...
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bioRxiv - Immunology 2019Quote: ... and a mixture of 8 VH family and IgM-specific primers (listed below) containing 5’ adaptors to allow barcoding by the Illlumina TruSeq multiplex pcr kit (Illumina). Resulting PCR products were subjected to 10 cycles of PCR using Illumina TruSeq indexing primers ...
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bioRxiv - Microbiology 2019Quote: ... universal bacterial 16S rRNA genes were PCR amplified using 926F and 1392wR primers and then sequenced on an Illumina MiSeq using 30% PhiX Control v3 (Illumina) and a MiSeq Reagent Kit v3 (600 cycles ...
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bioRxiv - Evolutionary Biology 2019Quote: ... we subjected the gel-purified product of the first PCR reaction to a second PCR reaction using a pair of index primers from Nextera XT DNA library preparation kit (Illumina). Fourth ...
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bioRxiv - Microbiology 2019Quote: The 2nd round barcoded PCR was performed by using sixteen forward and twenty-four reverse barcoded primers obtained from Illumina MiSeq protocols (S2 Table) ...
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bioRxiv - Cell Biology 2020Quote: To the cancer hot spot library P5 and P7 sequences were attached by PCR using the custom pBB9 primer and Nextera N701 (Illumina). Library was purified with 0.6x volume fraction AMPure beads ...
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bioRxiv - Genomics 2021Quote: ... Bisulfite-treated DNA samples (50 ng) were subjected to 10 cycles of PCR using random primers from the TruSeq DNA Methylation Kit (Illumina) to add adapters ...
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bioRxiv - Developmental Biology 2021Quote: ... 25 mL of 2x NEBNext Ultra II Q5 Master Mix, 3 mL of 10 mM Universal PCR primer from Illumina, 3 mL of 10mM Index PCR primer and 9 mL of nuclease-free water and amplified for 10 cycles (98C for 10 s ...
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bioRxiv - Microbiology 2021Quote: ... Libraries were prepared following the RNA-seq sample work flow using the Scriptseq Complete kit for bacteria and indexed with unique Scriptseq Index PCR primers (Illumina).
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bioRxiv - Genomics 2021Quote: ... DNA was PCR amplified for a total of 11-13 cycles using barcoded primers (Illumina Nextera XT Index Kit v2) and purified using Ampure beads (1.4:1 beads:sample ratio) ...
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bioRxiv - Immunology 2022Quote: ... Purified DNA was further amplified by PCR using Kapa Hifi Hotstart Ready Mix with Nextra XT Index Primers from Nextra XT Index Kit (Illumina). The PCR condition was ...
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bioRxiv - Microbiology 2022Quote: ... Gel-extracted DNA (size selection ~250 to 300bp) was amplified by PCR using primers and cycling conditions recommended by Illumina. Purified PCR products were submitted to the UW-Madison Biotechnology Center for a single end 50-bp Illumina MiSeq run ...
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bioRxiv - Genetics 2023Quote: ... Locus specific PCR product was used for indexing PCR using KOD Hot Start DNA Polymerase and TruSeq i5 and i7 indexing primers (Illumina) following the cycling conditions ...