Labshake search
Citations for Illumina :
301 - 350 of 9201 citations for hsa mir 373 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... We prepare 12 cDNA libraries (3 individuals □ 2 sampling times (dawn and dusk) □ 2 localities) using the TruSeq RNA-seq library prep kit from Illumina (Illumina, Inc., CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... a custom pipeline using GATK tools and DRAGEN RNA Pathogen Detection software (Illumina). Sequences of the two COH.20G/501Y viruses were deposited to GISAID.org as EPI_ISL_832378 and EPI_ISL_826521 ...
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bioRxiv - Plant Biology 2020Quote: ... to an average size of 500-bp and indexing with sequencing adapters using the TruSeq DNA (PCR free) kit (Illumina). Sequencing was performed on a single lane of an Illumina HiSeq2500 with 500 cycles in high-output mode ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR amplicons were purified by gel extraction and the concentrations of each sample was adjusted to 25 ng/µl for the 2nd stage barcoding PCR using TG Nextera XT Index Kit v2 Set A (Illumina) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... RNA-seq libraries were prepared from 500ng of RNA samples using an oligo dT based method with 15 cycles of PCR amplification with the Illumina TruSeq mRNA stranded kit (Illumina) and sequenced on the Illumina HiSeq 2000 v4 (50-bp single end read) ...
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bioRxiv - Developmental Biology 2021Quote: ... Input material ranged from 500pg to 15ng of immunoprecipitated DNA and processed according to the kit guidelines (NEBNext End Prep, Adaptor Ligation, PCR enrichment of Adaptor-Ligated DNA using NEBNext Multiplex Oligos for Illumina). Clean up and size selection were performed with AMPure beads (NEB) ...
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bioRxiv - Developmental Biology 2022Quote: ... The barcode sequencing libraries were quantified by quantitative PCR (KAPA Biosystems Library Quantification Kit for Illumina platforms P/N KK4824). Sequencing libraries were loaded at 18 pM on an Illumina HiSeq2500 using the following read length ...
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bioRxiv - Genomics 2020Quote: ... Successful amplicons were screened on a 1.5% agarose gel and further purified using Agencourt AMPure XP PCR Purification kit before proceeding to Nextera XT DNA Library Preparation (Illumina), according to manufacture instructions ...
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bioRxiv - Genomics 2020Quote: ... DNA samples were processed using the Illumina TruSeq DNA PCR-Free Sample Preparation kit (Illumina Inc., San Diego, CA, USA) on the Hamilton Microlab Star (Hamilton Robotics ...
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bioRxiv - Genomics 2020Quote: ... Paired-end libraries were prepared by the Purdue Genomics Core Facility using the Truseq DNA PCR-Free Sample Preparation Kit (Illumina). Whole-genome sequencing was performed using Illumina short-read technology (2×100bp ...
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bioRxiv - Immunology 2019Quote: ... and by enzymatic fragmentation of 300 pg of cDNA followed by 12 PCR cycles using the Nextera XT DNA Library Preparation Kit (cat# FC-131-1096, Illumina). Sequencing of the 15 mRNAseq libraries multiplexed together was carried out with an Illumina HiSeq2500 on a 2x125 bp paired-end run.
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bioRxiv - Immunology 2019Quote: ... and a mixture of 8 VH family and IgM-specific primers (listed below) containing 5’ adaptors to allow barcoding by the Illlumina TruSeq multiplex pcr kit (Illumina). Resulting PCR products were subjected to 10 cycles of PCR using Illumina TruSeq indexing primers ...
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bioRxiv - Genomics 2019Quote: ... and three mate-pair libraries (insert sizes of 2 kb, 5 kb, and 8 kb) were constructed using the TruSeq PCR-free Kit (Illumina) and Mate-pair Kit (Illumina) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... we subjected the gel-purified product of the first PCR reaction to a second PCR reaction using a pair of index primers from Nextera XT DNA library preparation kit (Illumina). Fourth ...
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bioRxiv - Genomics 2019Quote: ... and ligated to TruSeq LT adapters using a TruSeq DNA PCR-Free Library Preparation Kit (Illumina, San Diego, CA, USA). We purified the ligation reaction using a Qiaquick Gel Extraction Kit (Qiagen ...
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bioRxiv - Genomics 2021Quote: ... libraries with an insert size of 350 bp with the TruSeq DNA PCR-free gel-free library preparation kit (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... resequencing of genomic DNA was performed for accessions of ‘Kirakiraboshi,’ ‘Frau Yoshimi,’ ‘Posy Bouquet Grace,’ and ‘Blue Picotee Manaslu.’ Sequencing libraries (insert size of 500 bp) for the four lines were constructed with TruSeq DNA PCR-Free Library Prep Kit (Illumina) to sequence on a HiSeqX (Illumina) ...
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bioRxiv - Genetics 2019Quote: DNA sequencing libraries were constructed from 1 µg of genomic DNA using TruSeq DNA PCR-free Library Prep kit (Illumina). Sequencing was run on an Illumina HiSeq 2500 apparatus using a paired-end read length of 2×150 pb with the Illumina Reagent Kits as already described (Demars et al ...
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bioRxiv - Cancer Biology 2019Quote: WGS libraies were generated from 1 μg of 50 ng/ul high molecular weight gDNA using the TruSeq PCR-free Library Prep Kit (Illumina), according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... 1 ng of the resulting cDNA from 14 PCR cycles was “tagmented” and barcoded by using a Nextera XT DNA Sample Preparation Kit (Illumina). The final libraries were purified by AmPure XP magnetic beads (Beckman Coulter ...
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bioRxiv - Microbiology 2021Quote: ... Metagenomic libraries were prepared using 700 ng of DNA and the TruSeq DNA PCR-Free Library Prep Kit (Illumina Inc.) following the manufacturer’s recommended protocol ...
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bioRxiv - Microbiology 2020Quote: ... One microgram of gDNA with a DNA integrity number (DIN) of <=6 was used for library preparation using the TruSeq PCR-free library preparation kit (Illumina) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... The 9 genomic DNA pools for sequencing were prepared by mixing equimolar concentrations of DNA from which 9 libraries were prepared with an insert size of 350 bp using a TruSeq PCR-free sample prep kit (Illumina), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... In the second method purified PCR amplicons (100 ng) were used to prepare sequencing libraries by TruSeq Nano DNA LT Library Preparation Kit (Illumina) with the following modifications ...
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bioRxiv - Cell Biology 2021Quote: ... The generated cDNA was 3′ end-adenylated and ligated to Illumina Paired-end sequencing adapters and amplified by PCR using HiSeq SR Cluster Kit v4 cBot (Illumina). Libraries were analyzed on a 2100 Bioanalyzer (Agilent ...
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bioRxiv - Genomics 2022Quote: ... A library was prepared using a TruSeq DNA PCR-Free library preparation kit according to manufacturer guidance and was sequenced on a NextSeq500 instrument (Illumina) with a NextSeq High Output v2 300 cycle kit ...
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bioRxiv - Neuroscience 2022Quote: ... and finally converted into DNA libraries using custom rpi primers (RNA PCR Primer Index) adapted from the Truseq Small RNA kit (Illumina)106 ...
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bioRxiv - Genomics 2022Quote: ... A paired-end library was prepared with the TruSeq DNA PCR-Free LT Sample Preparation Kit (Illumina, #FC-121-3001) from 1 µg of the genomic DNA ...
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bioRxiv - Developmental Biology 2022Quote: ... Illumina sequencing libraries were prepared from the PCR-amplified cDNA from mutant lungs and controls using the Nextra DNA library prep kit (Illumina). Libraries were sequenced on a NovaSeq 6000 S Prime flowcell (Illumina ...
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bioRxiv - Microbiology 2020Quote: Sequencing libraries were prepared for each product of long PCR (ranging from 15 to 33 kb) using the Nextera XT DNA Sample Preparation Kit (Illumina) and sequenced on the Illumina MiSeq platform to generate paired-end (PE ...
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bioRxiv - Genomics 2019Quote: ... and then subjected to library construction using a TruSeq DNA PCR-Free HT sample prep kit (Illumina; San Diego, CA). The libraries were sequenced on a HiSeq 2500 system (Illumina ...
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bioRxiv - Microbiology 2021Quote: The second stage PCR procedure attached unique index primers to each sample using the Nextera XT v2 set A kit (Illumina). Purified DNA (5 μl ...
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bioRxiv - Microbiology 2021Quote: ... DNA libraries preparation with 16 cycles of PCR amplification and sequencing were done following manufacturer instructions (TruSeq ChIP-Library kit, NextSeq 500/550, Illumina).
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bioRxiv - Microbiology 2021Quote: ... Sequencing libraries were prepared from 100 ng of DNA with the TruSeq DNA PCR-Free library Prep Kit from Illumina and sequenced on Illumina MiSeq platform with 150-bp paired-end read lengths (Institut Pasteur ...
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bioRxiv - Neuroscience 2020Quote: ... then the second-stage PCR was performed to attach Illumina i7 and i5 indexes (Illumina Nextera XT Library Preparation Kit) to individual samples ...
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bioRxiv - Cancer Biology 2022Quote: ... performed the library preparation for WGS of parental and resistant cells of cell lines #3 and #9 as well as a control tail samples corresponding to line #3 with the TruSeq DNA PCR-free Kit (Illumina) and the 150 bp paired-end sequencing on a HiSeq X (Illumina).
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bioRxiv - Microbiology 2020Quote: ... Sequencing libraries were prepared from 100 ng of DNA with the TruSeq DNA PCR-Free library Prep Kit from Illumina and sequenced on Illumina MiSeq platform with 150-bp paired-end read lengths (Institut Pasteur ...
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bioRxiv - Developmental Biology 2020Quote: ... A paired-end library with an average insert size of 450 bp was constructed with a TruSeq DNA PCR-free Library Prep Kit (Illumina) and two different mate-pair libraries (3 kb and 5 kb ...
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bioRxiv - Evolutionary Biology 2019Quote: ... A paired-end 150 bp library was prepared for each sample using the TruSeq DNA PCR-Free Library Prep Kit (Illumina), and sequenced on the Illumina HiSeq X platform ...
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bioRxiv - Molecular Biology 2021Quote: ... The generated cDNA was 3′ end-adenylated and ligated to Illumina Paired-end sequencing adapters and amplified by PCR using HiSeq SR Cluster Kit v4 cBot (Illumina). Libraries were analyzed on a 2100 Bioanalyzer (Agilent ...
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bioRxiv - Molecular Biology 2020Quote: ... Pools were sent to the University of Geneva for sequencing Illumina (MiSeq) where sequencing libraries were prepared using the reagents of the PCR-free TruSeq kit (Illumina).
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bioRxiv - Molecular Biology 2020Quote: ... The generated cDNA was 3′ end-adenylated and ligated to Illumina Paired-end sequencing adapters and amplified by PCR using HiSeq SR Cluster Kit v4 cBot (Illumina). Libraries were analyzed on a 2100 Bioanalyzer (Agilent ...
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bioRxiv - Immunology 2019Quote: ... adapter ligation and PCR amplification were performed using the TruSeq stranded total RNA with ribo-zero globin sample preparation kit (Illumina). The sequencing of transcriptome libraries was performed on the Illumina HiSeq 1500 platform (Illumina ...
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bioRxiv - Microbiology 2019Quote: ... Library preparation was carried out by the IBEST Genomic Resources Center at the University of Idaho (Moscow, ID): genomic DNA was used to make shotgun libraries using the TruSeq PCR-free Library Prep Kit (Illumina) with the HiSeq-length insert option (short) ...
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bioRxiv - Genetics 2021Quote: ... 700 ng of genomic DNA was sheared to an average size of 400 bp using a Covaris LE220 and was used as input to generate a whole-genome library using the TruSeq PCR-free kit (Illumina). The resulting DNA libraries were sequenced on Illumina Hi-seq X instrument to generate 2×150bp paired-end reads.
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bioRxiv - Genomics 2021Quote: ... Bisulfite-treated DNA samples (50 ng) were subjected to 10 cycles of PCR using random primers from the TruSeq DNA Methylation Kit (Illumina) to add adapters ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and subjected to a second PCR to introduce dual index sequences using Nextera XT Index Kits (Illumina, San Diego, CA). In the second PCR ...
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bioRxiv - Genomics 2021Quote: ... of Korea) for library preparation with 350 bp targeted insert size using TruSeq DNA PCR Free preparation kit (Illumina, USA) and sequencing on HiSeq X device (Illumina ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Subsequent to second round PCR amplification MiSeq sequencing libraries were generated using Nextera XT DNA Sample Preparation Kits (Illumina, Inc.). Amplicons for each sample were tagged using Nextera XT Index Kits (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... Purified PCR products were combined in equimolar concentrations and sequenced on an Illumina MiSeq (Miseq Reagent Kit V2, 50-cycle; Illumina) using primer PLM49 ...