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Citations for Illumina :
101 - 150 of 1391 citations for hsa let 7f RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Tagmented DNA fragments were enriched by 10 cycles of PCR amplification using PCR master mix and primers with the index from Illumina. Libraries were quantified by the KAPA SYBR fast quantitative PCR kit (Life Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... sequencing adaptors P5 and P7 were introduced following the 10xGenomics dual-index setup: 20-50ng PCR product was added to library PCR mix (50% KAPA, 10% Betaine, 20% primer Dual index (Illumina)) ...
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bioRxiv - Neuroscience 2024Quote: ... sequencing adaptors P5 and P7 were introduced following the 10xGenomics dual-index setup: 20-50ng PCR product was added to library PCR mix (50% KAPA, 10% Betaine, 20% primer Dual index (Illumina)) total 20µl ...
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bioRxiv - Neuroscience 2024Quote: ... sequencing adaptors P5 and P7 were introduced following the 10xGenomics dual-index setup: Eluted PCR product was added to library PCR mix (50% KAPA, 10% Betaine, 20% primer Dual index (Illumina)) ...
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bioRxiv - Developmental Biology 2021Quote: ... a 9-cycle PCR was performed using indexed primers from Nextera Index Kit (Illumina) and KAPA HiFi HotStart ReadyMix (KAPA Biosystems) ...
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bioRxiv - Molecular Biology 2020Quote: ... Eluted DNA was amplified with PCR using Nextera i7- and i5-index primers (Illumina). Purification and size selection of the amplified DNA were carried out with AMPure XP magnetic beads (Beckman Coulter ...
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bioRxiv - Microbiology 2021Quote: ... beads and multiplexed by using ScriptSeq Index PCR Primers (Epicentre, Illumina, Madison, WI USA). cDNA libraries were quantified by using KAPA Illumina Library Quantification kit (KAPA Biosystems ...
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bioRxiv - Genetics 2022Quote: ... The primers sequences were modified to include Illumina Nextera index PCR adaptor sequences (Illumina).
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bioRxiv - Neuroscience 2020Quote: ... and 5 µL of the Illumina PCR Primer Cocktail (PPC, Illumina FC-121-1030). PCR conditions were as follows ...
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bioRxiv - Genetics 2022Quote: ... both the SNP STARRseq samples were PCR-amplified with TruSeq dual indexing primers (Illumina) to generate Illumina compatible libraries ...
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bioRxiv - Cell Biology 2019Quote: ... remaining DNA was PCR-amplified with sequencing primers and used for sequencing (Illumina Hiseq2500). Raw reads were preprocessed using the FASTX-Toolkit ...
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bioRxiv - Developmental Biology 2020Quote: ... Eluted DNA was amplified with PCR using Nextera i7- and i5-index primers (Illumina) and purified with AMPure XP magnetic beads (Beckman Coulter) ...
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bioRxiv - Microbiology 2023Quote: ... 1 μl of forward and 1 μl of reverse 25 μM PCR primers (Illumina), and 0.5 μl of Phusion high-fidelity DNA polymerase (New England Biolabs) ...
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bioRxiv - Immunology 2024Quote: ... The products were then purified and enriched by PCR using paired-end primers (Illumina) for 15 cycles to create the final cDNA library ...
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bioRxiv - Microbiology 2021Quote: ... Fragments were then indexed with 10 nucleotide barcode sequences using Nextera XT Index Kit v2 Set D primers (Illumina, San Diego, California, USA). Indexing PCR reactions were run in triplicate with a volume of 25 μL using 12.5 μL NEB Next High-Fidelity Master Mix ...
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bioRxiv - Microbiology 2023Quote: ... The V3-V4 region of the 16S ribosomal RNA gene was amplified by PCR with universal bacterial primer sets (5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG-3’ and 5’-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC-3’) and was sequenced using MiSeq Reagent kit v3 (600 cycle) (Illumina Inc., California, US). The sequence data were analyzed using QIIME2 (https://qiime2.org/ ...
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bioRxiv - Bioengineering 2020Quote: ... the V3-V4 hypervariable region of the bacterial 16S rRNA gene pool of the DNA extracts was amplified by PCR using the pair of 341f/806r primers prior to sequencing of PCR products using a HiSeq 2500 sequencer (Illumina, USA). A detailed description of the wet-lab and dry-lab workflows can be found in Supporting Information S5.
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bioRxiv - Immunology 2021Quote: ... Transposed DNA fragments were purified using Qiagen Mini- Elute Kit and PCR amplified using NEB Next High Fidelity 2x PCR master mix (New England Labs) with dual indexes primers (Illumina Nextera). Genomic Alignment of sequencing reads ...
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bioRxiv - Microbiology 2019Quote: ... set A-D (Illumina). Bead-purified libraries were normalized and pooled (384 samples per run ...
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bioRxiv - Genetics 2023Quote: ... Set A (Illumina, USA). Samples were sequenced in two batches (S4 Table) ...
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bioRxiv - Microbiology 2023Quote: ... Set A (Illumina 20040553) and sequenced on a NovaSeq 6000 using a NovaSeq 6000 SP Reagent Kit v1.5 (100 cycles ...
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bioRxiv - Microbiology 2023Quote: ... Set A (Illumina 20040553). Libraries were assessed for cDNA quantity and library quality using Qubit and BioAnalyzer ...
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bioRxiv - Neuroscience 2023Quote: ... Set A (Illumina, USA). Barcoded cDNA libraries were pooled and sequenced on Illumina NextSeq 500 instrument using NextSeq 500/550 High Output 75 Cycles Kit v2.5 (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... set A indexes (Illumina) using Kapa HiFi master mix (Roche ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA from transposed nuclei were then isolated and PCR-amplified using barcoded Nextera primers (Illumina). Library quality control and quantitation was carried out using high-sensitivity DNA Bioanalyzer and Qubit ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Adapter extension was performed over 12 PCR cycles using TruSeq-compatible indexed primers (Illumina, Inc.) and Phusion high-fidelity taq polymerase (New England Biolabs ...
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bioRxiv - Bioengineering 2021Quote: ... and sequencing indices were added with a second round of PCR using indexing primers (Illumina), followed by a second SPRI cleanup ...
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bioRxiv - Microbiology 2020Quote: ... Unique barcodes were added to each sample library using ScriptSeq™ Index PCR Primers (Illumina). Libraries were analyzed on a Tapestation using HS D1000 tapes and reagents (Agilent ...
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bioRxiv - Cancer Biology 2020Quote: ... an Illumina compatible library was generated by PCR amplification with TruSeq dual indexing primers (Illumina) and sequenced on Illumina HiSeq4000 (150bp ...
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bioRxiv - Cancer Biology 2021Quote: Four 3’ PCR primers were used each containing a unique index (underlined) recognized by Illumina:
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bioRxiv - Genetics 2020Quote: ... Purified transposed DNA was amplified by PCR using Nextera barcoded primers (Illumina, San Diego, CA) and NEBNext High-Fidelity 2X PCR Master mix (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... A single round of PCR was performed using “fusion primers” (Illumina adaptors + indices + specific regions) targeting either the 16S V4-V5 (Bacteria ...
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bioRxiv - Evolutionary Biology 2022Quote: ... An initial multiplex PCR containing locus-specific primers with Illumina (Illumina, Inc., San Diego, CA) priming sites for 229 amplicons was performed ...
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bioRxiv - Genomics 2022Quote: ... 5μL PCR Primer Cocktail from the Illumina TruSeq Nano DNA LT Kit (Illumina, Cat#: 20015964) and 25μL sample ...
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bioRxiv - Genomics 2023Quote: ... nine-cycles of PCR were performed using indexed primers from a Nextera Index kit (Illumina), and amplified DNA was size-selected to a size of <500 bp using AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Neuroscience 2023Quote: ... An initial PCR amplification was performed on the tagmented DNA using Nextera indexing primers (Illumina). Real-time (RT)-qPCR was run with a fraction of the tagmented DNA to determine the number of additional PCR cycles needed ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA fragments were then amplified by PCR using Nextera index primer 1 and 2 (Illumina) and produced by PCR amplification (10–13 cycles ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR reactions were performed using primers from Nextera XT Index Kit (Illumina, FC-131-1001) and NEBNext High Fidelity 2X PCR Master Mix (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA from transposed nuclei was then isolated and PCR-amplified using barcoded Nextera primers (Illumina). Library QC was carried out using high sensitivity DNA bioanalyzer assay and Qubit measurement and sequenced using paired-end sequencing (PE50 ...
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bioRxiv - Cancer Biology 2023Quote: CUT & RUN libraries were prepared using the TruSeq ChIP-Sample Prep Kit-Set A and PCR Box (Illumina) from 5 ng of DNA with 10 PCR cycles for amplification following the manufacturer’s procedures ...
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bioRxiv - Immunology 2024Quote: ... The PCR products were then tagged using the Nextera XT Index Kit v2 Sets A to B (Illumina), with the amplicon concentrations normalized to 200 ng/µl via the Qubit high sensitivity dsDNA assay before pooling ...
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bioRxiv - Genomics 2023Quote: ... and 1.25µM indexing primers (Ad1_noMX primer and Ad2.x indexing primer 45 or IDT for Illumina dual index primers (Illumina, Nextera DNA UD Indexes Set A Ref 20025019) ...
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bioRxiv - Developmental Biology 2021Quote: ... The library was amplified by 18-cycle PCR using indexed primers from Nextera Index Kit (Illumina) and KAPA HiFi HotStart ReadyMix (KAPA Biosystems) ...
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bioRxiv - Molecular Biology 2020Quote: ... The resulting cDNA was finally PCR-amplified (12 cycles) with TruSeq Dual Index sequencing primers (Illumina) and Herculase II Fusion DNA Polymerase (Agilent) ...
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bioRxiv - Genomics 2019Quote: ... DNA fragments were subjected to 11 cycles of PCR amplification with Nextera dual index primers (Illumina) and the NEBNext High Fidelity 2X PCR Master Mix (New England Biolabs ...
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bioRxiv - Developmental Biology 2020Quote: ... The libraries then were amplified using PCR Enhancer mix and primer cocktail (Illumina, FC-121-4001) for 12 cycles ...
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bioRxiv - Genomics 2019Quote: ... Samples were then amplified by PCR with Illumina primers (NEBNext Multiplex Oligos for Illumina cat. E7335) in a 50⍰ μl reaction with 2× PCR master mix (NEB cat ...
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bioRxiv - Developmental Biology 2020Quote: ... and subsequently another 8-12 PCR cycles with the specific primers (Illumina Nextera XT index kit); samples were cleaned with PCR purification kit (Qiagen ...
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bioRxiv - Immunology 2021Quote: ... Add 30 μl PCR master mix (2.5 μl Illumina dual indexes primer 1, 2.5 μl Illumina dual index primer 2 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The second step of PCR used standard Nextera XT Index v2 primers (Illumina #FC-131-2004) to further label samples representing different conditions and timepoints with unique identifiers that allow for multiplexing on the same sequencing lane ...