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Citations for Illumina :
1 - 50 of 313 citations for Water Nuclease Free since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2021Quote: ... 22 μL nuclease-free-water) (Illumina FC-121-1030 Nextera DNA library prep kit) ...
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bioRxiv - Molecular Biology 2022Quote: ... 5.4μL nuclease-free water and 2.5μL TDE1 (Tn5 enzyme, Illumina)] followed by incubation at 37°C for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... 22 μl of nuclease-free water) (#20034197, Illumina; #G9441, Promega) for incubattion at 37°C with shaking at 300 rpm for 30 min ...
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bioRxiv - Cancer Biology 2021Quote: ... 19.5µl of nuclease-free water) (FC-121-1030, Illumina; G9441, Promega). Transposition reactions were incubated at 37°C for 30 minutes in an Eppendorf ThermoMixer with agitation at 1000 RPM ...
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Heatrich-BS enables efficient CpG enrichment and highly scalable cell-free DNA methylation profilingbioRxiv - Genomics 2021Quote: ... 1μl of nuclease-free water and 1μl of 750nM methylated Truseq adapter (Illumina). For no-heat controls ...
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bioRxiv - Cancer Biology 2020Quote: ... 2.5μL transposase and 22.5μL nuclease-free water (Nextera DNA Library Prep, Illumina #FC-121-1030) or in 50μl of tagmentation mix containing 25μL of TAPS-DMF buffer (80mM TAPS ...
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bioRxiv - Immunology 2020Quote: ... and nuclei were resuspended in 50μl of transposition reaction mix (TD buffer [25μl], Tn5 Transposase [2.5μl], nuclease-free water [22.5μl]; (Illumina)) and incubated for 30min at 37°C ...
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bioRxiv - Immunology 2022Quote: ... and nuclei were resuspended in 50μl of transposition reaction mix (TD buffer [25μl], Tn5 Transposase [2.5μl], nuclease-free water [22.5μl]; (Illumina)) and incubated for 30min at 37°C ...
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bioRxiv - Genetics 2020Quote: ... and 22.5 μl nuclease-free water) (Nextera DNA Library Prep Kit (Illumina, cat. no. FC-121-1030). The transposase reaction was performed at 37°C for 30 minutes ...
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bioRxiv - Genetics 2021Quote: ... 50,000 nuclei were incubated in the transposition reaction mix (20μl nuclease-free water; 25μl 2X Tagment DNA Buffer, Illumina Cat#FC-121-1030 ...
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bioRxiv - Genomics 2021Quote: ... Beads were then re-suspended in the tagmentation mix (19 μl tagmentation buffer + 1μl Tagment DNA enzyme supplemented with 5μl nuclease-free water) from the Nextera DNA Sample Prep kit (Illumina) and incubated at 37°C for 10 minutes in a thermocycler ...
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bioRxiv - Cell Biology 2020Quote: ... 5 μl Tagment DNA Enzyme and 20 μl nuclease free water (Nextera DNA Sample Preparation Kit, Illumina, UK) for 45 min at 37°C ...
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bioRxiv - Genomics 2020Quote: ... 19.5 uL of nuclease-free H20) (Illumina, Nextera DNA library Preparation kit FA-121-1030 ...
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bioRxiv - Developmental Biology 2021Quote: ... The nuclei pellet was then resuspended in the 50ul of the transposition reaction mix (25ul Tagment DNA buffer, 2.5ul TD Tn5 Transposase enzyme, 22.5ul nuclease-free water) (Illumina; 20034197). The transposition reaction was incubated at 37°C for 30 min on a thermomixer with constant gentle shaking at 1000 rpm ...
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bioRxiv - Cell Biology 2021Quote: ... was performed by incubating beads with tagmentation buffer (12.5 µl 2 x TD buffer, 11.5 µl nuclease free water, 1µl Tn5 enzyme (Illumina #FC-131-1024)) at 37°C for 10min ...
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bioRxiv - Neuroscience 2021Quote: ... RNA extracted from sorted mouse brain hemisphere microglia was eluted in 30 μl nuclease-free water and subjected to 50 bp single-end mRNA sequencing using HiSeq 4000 (Illumina). Raw sequencing data were first evaluated by FASTQC v0.72 for quality ...
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bioRxiv - Microbiology 2021Quote: ... DNA was diluted to 0.2 ng/μL in nuclease-free water and processed for sequencing using the Nextera XT DNA Library Prep Kit (Illumina).
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bioRxiv - Systems Biology 2020Quote: ... DNA was diluted to 0.2 ng/uL in nuclease-free water and processed for sequencing using the Nextera XT DNA Library Prep Kit (Illumina).
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bioRxiv - Genetics 2021Quote: ... 50,000 nuclei were incubated in the transposition reaction mix (20μl nuclease-free water; 25μl 2X Tagment DNA Buffer, Illumina Cat#FC-121-1030; 5μl Tn5 Transposase, Illumina Cat#FC-121-1030) at 37°C for 30 minutes ...
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bioRxiv - Genomics 2020Quote: ... The resulting nuclei were resuspended in 50µl of transposition reaction buffer (25µl of 2x TD buffer from the Nextera kit, Illumina; 2.5µl of Tn5 transposase enzyme from the Nextera kit, Illumina; 22.5µl of nuclease free water), and incubated at 37°C for 90 min for chromatin tagmentation ...
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bioRxiv - Genetics 2020Quote: ... and the nuclei was resuspended with Tn5 transposase reaction mix (25◻μl 2◻×◻TD buffer, 2.5◻μl Tn5 transposase and 22.5◻μl nuclease-free water) (Illumina) at 37◻°C for 30 min ...
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bioRxiv - Genomics 2020Quote: ... 22.5 μl nuclease-free dH2O) from the Nextera DNA sample kit (FC-121-1031, Illumina). The transposition reaction was performed at 37°C for 30 min ...
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bioRxiv - Molecular Biology 2019Quote: ... 2.5 µL Tn5 transposase and 22.5 µL nuclease-free H2O) using reagents from the Nextera DNA library Preparation Kit (Illumina #FC-121-103). Samples were then incubated at 37°C for 30min ...
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bioRxiv - Genetics 2021Quote: ... The nuclei were resuspended on ice in the transposition reaction mix (2x TD Buffer, 2.5μL Tn5 Transposes and Nuclease Free H2O) (Illumina Cat #FC-121-1030, Nextera) on ice and the transposition reaction was incubated at 37°C for 45 min ...
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bioRxiv - Genetics 2021Quote: ... DNase- and protease-free molecular grade water) was sent to GATC (Eurofins) for sequencing (Genome Sequencer Illumina HiSeq).
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bioRxiv - Genomics 2019Quote: ... 15 to 30 U TruSeq Nuclease (Illumina) was used to digest 30 to 60 µg RNA in up to 300 µL lysate ...
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bioRxiv - Systems Biology 2020Quote: ... Supernatants were discarded and nuclei were re-suspended in 50 μl reaction buffer (2.5 μl of Tn5 transposase and 25 μl of TD buffer from a Nextera DNA Library Prep Kit from Illumina, and 22.5 μl nuclease-free H2O). The reaction was incubated at 37°C for 30 min ...
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bioRxiv - Genomics 2023Quote: The 200 µL of the lysates described above were treated with RNase I (50 units nuclease per 40 µg of RNA; the nuclease was included in TruSeq Mammalian Ribo Profile Kit, Illumina, RPHMR12126) for 1 hour at room temperature with gentle mixing ...
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bioRxiv - Genomics 2023Quote: ... after RNase I digestion (50 units nuclease per 40 µg of RNA; the nuclease was included in TruSeq Mammalian Ribo Profile Kit, Illumina, RPHMR12126), adding 15 µl of SUPERase-IN (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... 22.5 μl water) (Illumina Cat# 20031198) was added to the nuclear pellet ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... 5 µl nuclease and 2.5 µl Tn5 transposase enzyme (TDE1, Illumina, catalog # 15027865) and incubated for 28 minutes at 37°C ...
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bioRxiv - Genomics 2019Quote: ... gel-free protocol (Illumina). The libraries were size-selected to an average of 500 bp using AMPure XP beads (Beckman Coulter ...
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bioRxiv - Evolutionary Biology 2019Quote: TruSeq DNA PCR-Free (Illumina) sequencing libraries were prepared ...
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bioRxiv - Genetics 2021Quote: ... 5 µl water) with 2.5 µl transposase (Illumina 20034197) for 30 min at 37 °C with shaking at 1000 r.p.m ...
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bioRxiv - Genetics 2021Quote: ... TruSeq DNA PCR Free kit (Illumina) was used for the whole-genome sequencing library preparation ...
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bioRxiv - Genomics 2020Quote: ... PCR-free sequencing libraries were generated using Illumina TruSeq DNA PCR-Free Library Preparation Kit (Illumina, USA) following the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2023Quote: PCR-free sequencing libraries were generated using the Illumina TruSeq DNA PCR-Free Library Preparation Kit (Illumina) following the manufacturer’s recommendations ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCR-free sequencing libraries were generated using the Illumina TruSeq DNA PCR-Free Library Preparation Kit (Illumina) following the manufacturer’s recommendations ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 100bp TruSeq DNA PCR-free libraries (Illumina) were prepared according to manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... TruSeq DNA PCR free (Illumina Inc., USA) and Collibri PCR free PS DNA (ThermoFisher ...
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bioRxiv - Genomics 2023Quote: ... 100bp TruSeq DNA PCR-free libraries (Illumina) were prepared according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... a 200 µL aliquot of RNA was treated with 50 units of nuclease provided by the ARTSeq/TruSeq Ribo Profile kit (Illumina) for an hour at 23°C on a nutator ...
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bioRxiv - Genomics 2021Quote: ... libraries with an insert size of 350 bp with the TruSeq DNA PCR-free gel-free library preparation kit (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... individual 100bp TruSeq DNA PCR-free libraries (Illumina) were sequenced with Illumina HiSeq 2500 high-throughput paired-end sequencing technology at the Lausanne Genomic Technologies Facility (GTF ...
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bioRxiv - Plant Biology 2020Quote: ... Library preparation (Illumina Shotgun TruSeq DNA PCR-free) and Illumina sequencing on a HiSeq X platform with a 150bp PE read length was performed by Macrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... Paired-end libraries (Illumina TruSeq DNA, PCR-free) were made according to the standard Illumina protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... PCR-Free kit (Illumina, San Diego, California, USA) and pooled samples sequenced on the HiSeq4000 platform (Illumina) ...
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bioRxiv - Genomics 2021Quote: ... and ligated to custom CpG-free annealed adapters (Oligo 3 - Custom CpG-free P7 adapter and Oligo 4 - Custom CpG-free P5 adapter; annealed according to the standard Illumina protocol). The adapter-ligated gDNA was purified and amplified in 20 reactions using KAPA HiFi master mix (Roche ...
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Blind exploration of the unreferenced transcriptome reveals novel RNAs for prostate cancer diagnosisbioRxiv - Genomics 2019Quote: ... cDNA libraries were normalized using an Illumina Duplex-specific Nuclease (DSN) protocol prior to a paired-end sequencing on HiSeq™ 2500 (Illumina). At least 20x coverage per sample was considered as minimum of unique sequences for further data analysis.
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bioRxiv - Plant Biology 2019Quote: ... 100 µL of the lysate described above was treated with 100 units of nuclease (provided in the TruSeq Mammalian Ribo Profile Kit, Illumina RPHMR12126) per 40 µg of RNA with gentle shaking at room temperature for one hour ...