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Citations for Illumina :
51 - 100 of 886 citations for WD Repeat Containing Planar Cell Polarity Effector WDPCP Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Precipitated chromatin was washed and incubated in Tagmentation buffer containing Tn5 transposase enzyme (Illumina). Samples were reverse crosslinked for 4 hours at 65°C ...
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bioRxiv - Cancer Biology 2021Quote: Four 3’ PCR primers were used each containing a unique index (underlined) recognized by Illumina:
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bioRxiv - Genomics 2021Quote: ... Transposition reaction mix containing transposition buffer and 2.5 ul of Tn5 enzyme per sample (Illumina) was added to the pellet and incubated for 30 min at 37 C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... An initial multiplex PCR containing locus-specific primers with Illumina (Illumina, Inc., San Diego, CA) priming sites for 229 amplicons was performed ...
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bioRxiv - Synthetic Biology 2022Quote: ... adding barcodes to identify the sample (primers P3-P15 in Supplementary Table 3, containing Illumina Nextera tagmentation adapters and ...
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bioRxiv - Genomics 2023Quote: ... The nuclei were resuspended with 30 μl of loading buffer containing 1x TB1 (Illumina Inc.), 1x standard storage buffer (Illumina Inc.) ...
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bioRxiv - Synthetic Biology 2023Quote: ... The resultant recombinants containing known donor barcodes and unknown recipient barcodes were sequenced by Illumina MiSeq platform ...
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bioRxiv - Plant Biology 2024Quote: ... and second strand synthesis buffer (containing dNTPs, RNase H and E. coli polymerase I, Illumina) was added so that the second strand was produced by nick-translation ...
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bioRxiv - Neuroscience 2021Quote: ... resuspended in 25 μl of 1X transposition buffer containing 2 μl of Tagment DNA Enzyme (Illumina) and incubated at 37°C for 30 min in a thermocycler ...
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bioRxiv - Neuroscience 2020Quote: ... poly(A) containing mRNA was purified and libraries were prepared using TruSeq Stranded mRNA kit (Illumina). Unstranded libraries with a mean fragment size of 150bp (range 100-300bp ...
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bioRxiv - Cancer Biology 2022Quote: ... Illumina compatible adaptors (PE1/PE2) containing single-direction indexes (Access Array™ Barcode Library for Illumina® Sequencers-384 ...
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bioRxiv - Immunology 2023Quote: ... 10% v/v dimethylformamide) containing 1 μl Tagment DNA Enzyme (Nextera DNA Sample Prep Kit (Illumina)) ...
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bioRxiv - Neuroscience 2023Quote: ... and nuclei were resuspended in 200μl transposition mix (1x TD buffer containing X µl of Illumina Tagment DNA TDE1 Enzyme per every X nuclei) ...
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bioRxiv - Cancer Biology 2024Quote: ... Nuclei were resuspended in 50 μL tagmentation reaction containing 25 μL 2X tagmentation buffer (Illumina 15027866), 16.5 μL PBS ...
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bioRxiv - Genomics 2019Quote: ... Frozen nuclear pellets of 2.5 × 104 PSCs were thawed on ice and tagmented in total volume of 25μl in permeabilization buffer containing digitonin and 2.5μl of Tn5 from Nextera DNA Library Preparation Kit (Illumina) for 45-75min at 37°C in a thermomixer (500 RPM shaking) ...
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bioRxiv - Genetics 2022Quote: ... Individual sequencing barcodes were added to each sample by amplifying the 20 μL elution in a 50 μL Q5 NEBNext reaction with 0.5 uM of TruSeq_Universal_Adapter primer and a reverse primer containing a unique 8 bp index (Illumina_Multiplex) for sample demultiplexing post-sequencing ...
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bioRxiv - Cancer Biology 2022Quote: ... The nuclei were centrifuged at 500g for 5 minutes at 4°C and subsequently resuspended on ice in 50μl transposase reaction buffer containing 2.5μl of Tn5 transposase and 25μl of 2xTD buffer (Nextera DNA Sample preparation kit from Illumina). After incubation at 37°C for 30 minutes ...
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bioRxiv - Immunology 2020Quote: ... resuspended in 50 μl 1xTD buffer containing 2.5 μl TDE1 transposase (Illumina Nextera DNA Sample Preparation Kit), and incubated for 30 min at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... Beads were then resuspended in 25μL of tagmentation reaction mix containing 12.5μL of 2x Tagmentation DNA Buffer (Illumina #15027866) and 1μL of Tagment DNA Enzyme (Illumina #15027865) ...
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bioRxiv - Genomics 2020Quote: ... Nuclei pellets were collected by centrifugation and resuspended with transposition reaction buffer containing Tn5 transposases (Illumina Nextera TDE1). Transposition reactions were incubated at 37°C for 30 min ...
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bioRxiv - Cancer Biology 2021Quote: ... Nuclei were isolated from approximately 50,000 cells in lysis buffer containing 0.1% NP-40 and 0.01% Digitonin and were fragmented with Nextera Tn5 Transposase (TDE1, Illumina) in TD Tagment DNA buffer (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... to a final concentration of 2 nM containing 15% PhiX V3 library control (Illumina, San Diego, CA, USA), the library pools were denatured for 5 min in an equal volume of 0.2M NaOH ...
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bioRxiv - Genomics 2019Quote: ... 10% v/v dimethylformamide) containing 1 μl Tagment DNA Enzyme from the Nextera DNA Sample Prep Kit (Illumina) and incubated at 37°C for 10 min followed by 2x washing in RIPA-LS and TE (10mM Tris-HCL ...
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bioRxiv - Neuroscience 2021Quote: ... Nuclei were then resuspended in 50 ul 1x TD Buffer containing 2.5 ul Tn5 enzyme from Nextera (Illumina) and incubated for 30 min at 37°C ...
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bioRxiv - Genomics 2022Quote: ... Nuclei pellets were resuspended in 50 μL transposition reaction containing 2.5 μL Tn5 transposase (FC-121-1030; Illumina). The reaction was incubated in a 37°C heat block for 45 min ...
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bioRxiv - Microbiology 2022Quote: ... Six samples per condition containing 4 μg of total RNA were used for TruSeq mRNA library preparation (Illumina) after being treated with Globin-Zero Gold rRNA Removal Kit (Illumina) ...
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bioRxiv - Developmental Biology 2023Quote: ... Isolated nuclei were mixed with 50 µl of transposition reaction mix containing Tagment DNA Enzyme 1 (Illumina, 20034197). Samples were incubated using a thermo shaker at 37°C ...
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bioRxiv - Genomics 2023Quote: ... 12.5 μl of a master mix containing 7.5 μl of Nextera PCR Master Mix (Illumina, FC-131-1096) and 2.5μl of each Index primer i7 (Illumina ...
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bioRxiv - Cancer Biology 2024Quote: ... Nuclei were then resuspended in 50 μL transposition reaction mix containing 25 μL 2X Tagment DNA buffer (Illumina), 2.5 μL Tn5 transposase (Illumina) ...
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bioRxiv - Molecular Biology 2024Quote: ... Library of polyA-containing mRNA (350 bp size) was prepared using TruSeq Stranded mRNA Library Prep kit (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: Adapter sequences were trimmed from the raw reads using fastq-mcf (ea-utils v1.1.2, with default parameters and using a list containing the common Illumina adapters) and the quality of the cleaned reads was checked with FastQC (v0.1) ...
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bioRxiv - Microbiology 2021Quote: ... The amplicon library was diluted to 7 pM containing 20 % PhiX before sequencing on the MiSeq platform (Illumina, USA) using MiSeq reagent v3 kit to generate 300 bp paired-end reads ...
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bioRxiv - Molecular Biology 2022Quote: ... Supernatant was discarded and pellets resuspended in 20 μl ATAC reaction buffer containing 10 μl 2x transposase buffer and 2.5 μl Tn5 enzyme (Illumina). Samples were incubated at 37 °C for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... cDNA libraries containing pooled barcoded samples was run across two lanes of a HiSeq400 (Illumina, San Diego, CA, USA) on two separate runs for 150 bp paired-end reads (http://dnatech.genomecenter.ucdavis.edu/) ...
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bioRxiv - Microbiology 2022Quote: We sequenced the pooled sample containing PCR products using Illumina MiSeq technology (Illumina Reagent Kit v2, 500 reaction kit) at the Center for Bioinformatics and Genomics at Indiana University ...
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bioRxiv - Neuroscience 2022Quote: ... 0.8 ul of each normalized sample was mixed with 2.4 ul of tagmentation mix containing Tn5 Tagmentation enzyme (20034198, Illumina) and then incubated at 55°C for 12 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... bead-bound complexes were resuspended in tagmentation mixture (25µL reaction containing 1 µL of enzyme in 1X buffer, Illumina) and incubated at 37°C for 25minutes ...
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bioRxiv - Microbiology 2023Quote: ... we then sought to remove BioProjects containing pyrosequencing data and other sequencing instruments that use processes dissimilar from Illumina sequencing ...
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bioRxiv - Neuroscience 2023Quote: ... The pellet was resuspended in 200uL of Transposition Mix (1x TD buffer containing 20 U/mL Superase-In RNase Inhibitor, 40 U/mL RNasin ribonuclease inhibitor and 1.25 µl of Illumina Tagment DNA TDE1 Enzyme per every 100 000 nuclei ...
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A gene desert required for regulatory control of pleiotropic Shox2 expression and embryonic survivalbioRxiv - Developmental Biology 2023Quote: ... 50’000 nuclei were then pelleted at 500 RCF for 10 min at 4°C and resuspended in 50 pL transposition reaction mix containing 25 pL Nextera 2x TD buffer and 2.5 pL TDE1 (Nextera Tn5 Transposase; Illumina) (cat ...
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bioRxiv - Cancer Biology 2021Quote: ... and PCR products were gel purified and stored in 50 μL buffer containing 10mM Tris (pH 8.0) until submission to TruSeq® DNA library preparation (Illumina) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Nuclei were centrifuged at 500 g for 10 min and immediately resuspended in 25 µl of buffer containing 2.5 µl of Tn5 transposase (Illumina, #15027865) for a 30 min incubation at 37°C ...
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bioRxiv - Genetics 2021Quote: ... Individual sequencing barcodes were added to each sample by amplifying the entire 40 μL elution in a 100 μL Q5 NEBNext reaction with 0.5 μM of TruSeq_Universal_Adapter primer and a reverse primer containing a unique 8 bp index (Illumina_Multiplex, Supplementary Table 14) for sample demultiplexing post-sequencing ...
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bioRxiv - Genomics 2019Quote: ... and the transposition was performed by resuspension of nuclei in 50 µL of Transposition Mix containing 1X TD Buffer (Illumina) and 2.5 µL Tn5 (Illumina ...
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bioRxiv - Microbiology 2019Quote: ... containing 5µL of each index primer (Nextera XT Index kit v2 set A, B and D; Illumina, San Diego, CA), 10ul of purified PCR products (0-20ng/µL ...
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bioRxiv - Immunology 2019Quote: ... and a mixture of 8 VH family and IgM-specific primers (listed below) containing 5’ adaptors to allow barcoding by the Illlumina TruSeq multiplex pcr kit (Illumina). Resulting PCR products were subjected to 10 cycles of PCR using Illumina TruSeq indexing primers ...
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bioRxiv - Microbiology 2022Quote: ... The primers Uni530F and Uni907R (Nunoura et al., 2012) containing Illumina TruSeq adapter sequences (Illumina Inc., San Diego, CA, USA) were used for PCR ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Two paired-end Illumina libraries containing 250 and 600 bp insertions (DRR029525 and DRR029526) were constructed using a TruSeq DNA Sample Prep Kit (Illumina). Three mate-pair libraries with 3000 ...
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bioRxiv - Microbiology 2022Quote: ... Two clones that produce the Y113F substituted Gp8 were propagated twice on strains containing only pCas9-guide for further selection and genomes were sequence verified by Illumina sequencing as described below.
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bioRxiv - Genomics 2019Quote: ... 5 mM MgCl2) containing 1 μl Tn5 transposase from the Nextera DNA Library Prep Kit (15028212, Illumina, San Diego, USA) and incubated at 37°C for 10min ...