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Citations for Illumina :
51 - 100 of 102 citations for Sar1 Angiotensin II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... The amplified cDNA underwent library preparation using the NEBNext Ultra II DNALibrar kit and subject to sequencing by Illumina Hiseq 2500 platform ...
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bioRxiv - Genomics 2021Quote: ... and sequencing was performed using eight SMRT Cells on a PacBio Sequel II instrument (Illumina, San Diego, California, USA). This yielded 787.76 Gb of sequence data ...
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bioRxiv - Genomics 2021Quote: ... 30 μL of NEBNext Ultra II Ligation Master Mix, 1 μL of NEBNext Ligation Enhancer, 2.5 μL of NEBNext Adapter for Illumina) at 20 °C for 15 min. ...
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bioRxiv - Genomics 2023Quote: ... and sequenced by PacBio Sequel II. Hi-C libraries were prepared following a standard protocol (Belton et al. 2012) and sequenced by Illumina HiSeq 4000 (Illumina ...
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bioRxiv - Developmental Biology 2023Quote: Library preparation was performed according to manufacturer’s instructions (NEBNext® Ultra™ II DNA Library Prep Kit for Illumina) using the following modified library amplification program ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by strand-specific cDNA NGS library preparation (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina, NEB). The size of the resulting library was controlled by use of a D1000 ScreenTape (Agilent 2200 TapeStation ...
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bioRxiv - Cell Biology 2023Quote: ... Total RNA was used for library preparation with NEBNext Ultra-low input II Directional RNA Library Prep Kit (Illumina) and sequenced on an Illumina HiSeq with 2×150 bp sequencing configuration (Illumina).
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bioRxiv - Molecular Biology 2024Quote: ... and RNA-seq libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit from Illumina (NEB, E7770) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... was isolated from pooled E8.0-E8.5 stage embryos using Isogen II reagent (Nippongene) and subjected to RNA-seq library preparation using TruSeq Stranded mRNA LT Sample Prep Kit (Illumina) based on manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ChIP-seq and DNase-seq libraries were prepared from 1-5 ng ChIP DNA or DNase DNA samples using NEBNext Ultra II DNA library Prep Kit (Illumina). Libraries were sequenced on an Illumina NextSeq 500 with single-end or paired-end 75 bp reads.
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bioRxiv - Microbiology 2020Quote: ... A reference genome from strain QMA0248 was constructed using both long reads derived from a single Smrt Cell using the PacBio RS II system with P4C2 chemistry and short reads from Illumina HiSeq2000 derived from Nextera XT paired-end libraries as reported elsewhere (NCBI accession no ...
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bioRxiv - Cancer Biology 2020Quote: The RNA-seq libraries (n=3 per experimental group) were prepared using the NEBNext Ultra II RNA library prep kit from Illumina (New England Biolabs Inc. ...
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bioRxiv - Molecular Biology 2022Quote: ... MEFs and NPCs were used to prepare NGS libraries based on the NEB Next Ultra II directional RNA library preparation kit from Illumina. As default ...
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bioRxiv - Cancer Biology 2022Quote: ... Libraries were constructed using NEB polyA RNA ultra II and subsequently subjected to 150 cycles of sequencing on NovaSeq-6000 (Illumina). Adapters were trimmed using Cutadapt (v2.3) ...
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Peripheral neuropathy linked mRNA export factor GANP reshapes gene regulation in human motor neuronsbioRxiv - Neuroscience 2021Quote: The RNA-seq libraries were made with NEBNext Ultra II Directional (PolyA) kit and sequenced with NovaSeq SP 2×150bp (Illumina) at Biomedicum Functional Genomics Unit (FuGU) ...
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bioRxiv - Genetics 2021Quote: ... one 150 bp paired end (350 bp insert size) Illumina library was prepared from the same DNA (NEBNext Ultra II DNA Library Prep Kit for Illumina) and sequenced to approximately 140 fold coverage (24.6 Gb ...
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bioRxiv - Developmental Biology 2021Quote: ... 25 mL of 2x NEBNext Ultra II Q5 Master Mix, 3 mL of 10 mM Universal PCR primer from Illumina, 3 mL of 10mM Index PCR primer and 9 mL of nuclease-free water and amplified for 10 cycles (98C for 10 s ...
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bioRxiv - Microbiology 2020Quote: ... Libraries were prepared using the NEB Next Ultra II kit (New England Biotech, Ipswich, MA) and paired-end sequenced (2×150bp) on a NextSeq system (Illumina). Adaptors were removed using cutadapt v1.10 [28] and reads assembled using SPAdes v3.7.1 [29] ...
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bioRxiv - Genomics 2021Quote: ... using the TruSeq DNA PCR-free library prep kit with fragments of 150 bp and ii.) Nextera Mate Pair library prep with fragments of 2,000 bp (Illumina, USA). The short-read paired-end libraries were sequenced in two independent lanes and the mate-pair library in one lane using the Illumina HiSeq4000 platform (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: ... ChIP samples were analyzed by qPCR or subjected to library preparation according to the manufacturer’s protocol (NEBNext Ultra II DNA Library Prep Kit for Illumina, NEB) using Dual Index Primers (NEBNext Multiplex Oligos for Illumina ...
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bioRxiv - Developmental Biology 2022Quote: ... DNA was measured again with Qubit dsDNA HS Assay Kit and 65ng of mononucleosomal purified DNA was used for NEBNext Ultra II DNA Library Prep Kit (Illumina) with size selection ...
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bioRxiv - Genomics 2022Quote: ... where polyA libraries were prepared with the NEBNext Ultra II mRNA-Seq protocol and sequenced on a NovaSeq sequencer (Illumina) to obtain ∼60 million PE150 pairs per sample.
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bioRxiv - Plant Biology 2023Quote: ... cDNA libraries were constructed from 1 μg/sample of total RNA using library preparation kits (NEBNext Ultra II RNA Library Prep Kit for Illumina 7770L ...
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bioRxiv - Microbiology 2023Quote: The extracted DNA samples were prepared for Illumina next-generation sequencing using the NEBNext Ultra II FS DNA Prep kit from Illumina, part number E7805 ...
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bioRxiv - Genetics 2023Quote: ... Batch 1 sequencing libraries were prepared by a commercial vendor (Azenta/Genewiz) using the NEBNext Ultra II RNA Library Prep Kit (Illumina) and sequenced using 2 x 150 bp paired end reads ...
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bioRxiv - Neuroscience 2023Quote: ... Libraries were constructed using NEB polyA RNA ultra II and subsequently subjected to 150 cycles of sequencing on NovaSeq-6000 (Illumina). Adapters were trimmed using Cutadapt (v2.3) ...
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bioRxiv - Genomics 2023Quote: ... we prepared stranded cDNA libraries for 909 samples from 695 sepsis patients using NEB Ultra II LIbrary Prep kits (Illumina) with NEBNext Poly(A ...
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bioRxiv - Molecular Biology 2024Quote: ... Extracted total RNA was send to Azenta Life Sciences for library preparation (sRNA: NEBNext Small RNA Library Prep Set for Illumina; mRNA: NEBNext Ultra II RNA Library Prep Kit for Illumina) and paired-end Illumina sequencing (NovaSeq 2×150bp).
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bioRxiv - Genomics 2020Quote: The immunoprecipitated sample and the input were subjected to Nextera library preparation as described by the manufacturer (NEBNext Ultra II DNA library Prep Kit for Illumina, NEB). The quality and size of libraries and chromatin shearing fragments were checked by Agilent Bioanalyzer using Agilent DNA High Sensitivity Chips (Agilent Technologies) ...
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Impairment of a distinct cancer-associated fibroblast population limits tumour growth and metastasisbioRxiv - Cancer Biology 2020Quote: ... mice were injected intraperitoneally with 150 mg kg−1 D-luciferin (Caliper Life Sciences) in 100 μL and mice imaged in vivo using an IVIS imaging chamber (IVIS Illumina II). Luminescence measurements (photons/second/cm2 ...
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bioRxiv - Microbiology 2022Quote: ... Paired-end libraries were constructed using the Illumina Herculase II Fusion DNA Polymerase Nextera XT Index Kit V2 (Illumina, Eindhoven, Netherlands). Primers used for bacterial amplification were Bac341F (5’-CCTACGGGNGGCWGCAG-3’ ...
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bioRxiv - Plant Biology 2023Quote: ... from 100 to 1000 ng of total RNA using a stranded kit (Illumina TruSeq Stranded Total RNA Kit or NEB Next Ultra™ II Directional RNA Library Prep Kit for Illumina). The resulting data files contained paired end sequences (150 bp ...
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bioRxiv - Cancer Biology 2023Quote: Mice were injected intraperitoneally with 150 mg kg-1 D-luciferin (Caliper Life Sciences) in 100 μL and mice imaged in vivo using an IVIS imaging chamber (IVIS Illumina II). Luminescence measurements (photons per second per cm2 ...
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bioRxiv - Plant Biology 2024Quote: ... DNA eluted from Chromatin-immunoprecipitated was prepared for library construction by using NEBNext® Ultra™ II DNA Library Prep with Sample Purification Beads according to standard operating procedures and then sequenced using HiSeq2000 (Illumina). Paired-end raw reads were first mapped to the Arabidopsis genome (TAIR10 ...
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bioRxiv - Microbiology 2024Quote: ... mRNA was used to generate the cDNA library according to the NEBNext® UltraTM II RNA Library Prep Kit protocol (Illumina), which was then sequenced using the Illumina HiSeq 2000 system ...
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bioRxiv - Developmental Biology 2021Quote: The resulting 79,472,462 paired end reads (NCBI PRJNA690950) were combined with an older library (16,225,145 paired end reads sequenced on an Illumina GA II; NBCI SRR1145735) for annotation of protein coding regions.
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bioRxiv - Neuroscience 2021Quote: ... Hippocampus RNA-seq libraries were prepared in accordance with New England Biolab protocols (NEBNext_ Ultra II RNA library Prep kit for Illumina, NEB, USA). Briefly ...
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bioRxiv - Immunology 2022Quote: ... libraries for sequencing were prepared using the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® following the manufacturer’s instructions (Illumina). Quality of all RNA and library preparations were evaluated with BioAnalyser 2100 (Agilent) ...
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bioRxiv - Microbiology 2022Quote: ... The same amount of the seven purified 3-cycle PCR products were further amplified using Platinum SuperFi II and the index primers from Nextera XT Index Kit v2 (Illumina cat. 15052163). The PCR products were purified using 1.8× beads ...
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bioRxiv - Microbiology 2023Quote: ... Preparation of cDNA fragment libraries was performed using the NEBNext® UltraTM II Directional RNA Library Prep Kit for Illumina® (Illumina). Sequencing was carried out on the Illumina NovaSeq platform (10 M paired-end reads ...
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bioRxiv - Genomics 2023Quote: ... single-indexed and dual-indexed DNA libraries were generated using the NEBNextUltra II DNA Library Prep kit from Illumina (New England Biolabs). Single-indexed libraries were sequenced 50 nt single end reads on a HiSeq 2500 (Illumina ...
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bioRxiv - Developmental Biology 2023Quote: ... Approximately 300 ng of RNA was used for library prep (NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina) following standard kit procedure ...
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bioRxiv - Cell Biology 2022Quote: ... 1,500ng of RNA at 50ng/μl was processed using Ribo-Zero Gold purification (QIAseq FastSelect -rRNA HMR Kit #334387) and NEBNext® Ultra™ II RNA Library Prep Kit (Illumina #E7770L). cDNA was sequenced using paired-end 150bp chemistry on a HiSeq 4000 instrument (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... Libraries were prepared using the New England Biolabs NEBNext rRNA depletion kit (Bacteria) and NEBNext Ultra II Directional RNA library prep kit (Illumina, San Diego, CA). The libraries were quantified by KAPA qPCR library quantitation assays and sequenced paired-end using MiSeq platform (Illumina ...
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bioRxiv - Genetics 2020Quote: ... sequencer-ready fragments were size selected (∼200-500 bp) and the RAD-seq libraries sequenced on a Genome Analyzer II (Illumina, San Diego, CA), paired-end 2 x 100 bp mode ...
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bioRxiv - Genomics 2020Quote: Fragmented cDNA Sequencing libraries were generated from 100ng of Total RNA using NEBNext Ultra II with polyA isolation module (Illumina, San Diego, California, USA) according to manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Sequencing libraries were prepared using NEBNext Ultra II directional RNA library kit and paired-end sequenced (150bp) in an Illumina HiSeq3000 (Illumina Inc., San Diego, USA) instrument ...
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bioRxiv - Genetics 2019Quote: ... The coding and splice-site regions of genes susceptible to hereditary arrhythmia and cardiomyopathies(10) were sequenced for probands II:3 and III-7 using Illumina HiSeq2500 Analyzer (Illumina, San Diego, CA, USA)(11) ...
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bioRxiv - Immunology 2021Quote: ... Library preparation was done using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina with poly-A selection (Illumina, San Diego, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Amplicons of the V3-V4 region of the 16S rRNA gene and the and the ITS1 region and library prep were generated by Herculase II Fusion DNA Polymerase Nextera XT Index Kit V2 (Illumina, San Diego, CA, USA). The products of the library prep were validated by an Agilent Tapestation D1000 (Santa Clara ...