Labshake search
Citations for Illumina :
701 - 750 of 895 citations for PD L1 Mouse HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Libraries were generated from the resulting cDNA (0.2ng/ul per sample) using the Nextera XT DNA library preparation kit (Illumina, Cat.no. FC-131-1024) as previously described68 ...
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bioRxiv - Cancer Biology 2023Quote: ... The cell pellet was resuspended in Illumina TD buffer and 2.5 µl of Tn5 enzyme was added to the transposition reaction (Illumina, Cat # FC-121-1030). Nuclei were incubated at 37°C for 60 minutes and fragmented DNA was purified using the MinElute kit (Qiagen ...
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bioRxiv - Systems Biology 2023Quote: ChIP-seq and input libraries were sequenced on the Illumina NextSeq 500 system with a ∼20% phiX spike-in (Illumina, FC-110-3001) to generate 75 bp single-end reads (NextSeq 500/550 High Output v2 kit) ...
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bioRxiv - Cell Biology 2023Quote: ... prior to pooling and sequencing using the Illumina NextSeq 500 platform using a custom primer and the High Output v2 kit (75Lcycles) (Illumina, #FC-404-2005). The library loading concentration was 2.4 pM ...
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bioRxiv - Microbiology 2023Quote: ... The cDNA libraries were then fragmented and amplified for sequencing using the Illumina Nextera XT DNA Library Preparation kit (Illumina, FC-131-1096) using custom primers that enabled the specific amplification of only the 3’ ends ...
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bioRxiv - Immunology 2023Quote: ... Nuclei were resuspended in the Nextera transposition reaction mix (25 μL 2x TD Buffer, 2.5 μL Nextera Tn5 transposase (Illumina Cat #FC-121-1030), and 22.5 μL nuclease free H2O ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries were constructed by following a previous protocol that optimizes reagent usage for microbial genomes [85] with the Nextera DNA Library Preparation Kit (Illumina, FC-121-1030) and sequenced on an Illumina HiSeq 2500 (UNH ...
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bioRxiv - Developmental Biology 2023Quote: ... and transferred to 3.0 μl Tn5 transposome mixture (0.25 μl Tn5 transposome, 2.5 μl tagmentation buffer, Illumina, FC-121-1030; 0.25 μl H2O). The samples were incubated in 37 °C water bath for 30 min ...
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bioRxiv - Developmental Biology 2023Quote: ... The cDNA was then amplified using 10 cycles and subsequently (0.33 ng) used to construct a pool of uniquely indexed samples using the Nextera XT kit (Illumina, Cat# FC-131-1096). Finally ...
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bioRxiv - Genomics 2023Quote: ... Isolated nuclei were lysed and transposed for 30 minutes at 37°C using the prokaryotic Tn5 transposase system (Nextera DNA library kit, Illumina, FC-121–1030). Transposed DNA was then purified on Diapure columns (Diagenode Cat# C03040001) ...
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bioRxiv - Microbiology 2024Quote: ... Sequencing was performed using Pair-end 2×100 cycle mode on the Illumina NovaSeq 6000 system (NovaSeq 6000 S1 Reagent Kit v1.5 200 cycles Illumina, cat. no. 20028317; 0.5% of the PhiX control library, Illumina, cat. no. FC-110-3001) and the standard clustering procedure.
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bioRxiv - Immunology 2024Quote: ... paired-end libraries were constructed from 1 µg of the initial genomic material using the TruSeq DNA v2 Sample Prep Kit (Illumina, #FC-121-2001) as per the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Approximately 1 ng of cDNA per cell was transformed into a single-cell library using the Nextera XT DNA Library prep kit (Illumina FC-131-1096) and following the manufacturer’s instructions with slight modifications ...
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bioRxiv - Genomics 2024Quote: ... and the final sequencing library was amplified utilizing the KAPA HiFi HotStart Uracil+ ReadyMix PCR Kit along with index primers from the Nextera XT Index Kit (Illumina, FC-131-1001). The amplification protocol involved an initial step at 98°C for 45 seconds ...
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bioRxiv - Microbiology 2024Quote: ... using the standard procedure recommended by the manufacturer with a 0.5% addition of the PhiX control library (Illumina, cat. no. FC-110-3001).
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bioRxiv - Genomics 2020Quote: ... The remaining 1μl re-dissolved contents were tagmented using 0.6 μl TD Tagmentation buffer and 0.3 μl ATM Tagmentation enzyme from Nextera XT DNA Library Prep Kit (Illumina, catalog no. FC-121-1030) for 5min at 55 °C ...
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bioRxiv - Genomics 2020Quote: ... The pool was denatured with sodium hydroxide according to Illumina recommendation and spiked with 5% PhiX (PhiX control v3 Illumina Catalogue FC-110-3001) before loading (Fig 1).
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bioRxiv - Genomics 2019Quote: ... Genomic DNA were pooled in equimolar ratio according to sex and Pool-seq libraries were generated using the Truseq nano DNA sample prep kit (Illumina, ref. FC-121-4001) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... according to the instructions for qChIP-Seq and paired-end sequencing (2×75nt) was performed on a NextSeq 500/550 using a HighOutput v2 Kit for 150 cycles (Illumina #FC-404-2002, discontinued).
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bioRxiv - Genomics 2020Quote: ... according to the manufacturer’s instructions and paired-end sequencing (2 × 75 bp) was performed on a NextSeq 500/550 using a HighOutput v2 Kit for 150 cycles (Illumina #FC-404-2002, discontinued).
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bioRxiv - Genetics 2021Quote: Tagmentation plates were prepared by the combination of 420 uL of 1400 nuclei/uL solution with 540 uL 2X TD Buffer (Nextera XT Kit, Illumina Inc. FC-131-1024). From this mixture ...
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bioRxiv - Immunology 2021Quote: ... prior to pooling and sequencing on an Illumina NextSeq 500 sequencer utilizing a BRB-seq custom primer and the High Output v2 kit (75 cycles) (Illumina, Cat. #FC-404-2005). The sequencing configuration is as follows ...
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bioRxiv - Neuroscience 2019Quote: ... and 1% Input were generated for NDC and PSEN1M146L conditions at the UC San Diego IGM sequencing core using Illumina TruSeq LT adaptors (Illumina Cat. FC-121-2001). Sequencing was performed on an Illumina HiSeq4000 generating Single-Read ...
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bioRxiv - Cancer Biology 2020Quote: ... and poly-A sequencing libraries (HE2.1 and TIVE cells) were generated using the TruSeq RNA Sample Prep Kit (Illumina, catalog no. FC-122-1001).
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bioRxiv - Cancer Biology 2020Quote: ... The tagmented and multiplexed cDNA libraries were sequenced in Nextseq 500 using Nextseq 500 High Output v.2 kit (75 cycles, Illumina Cat# FC-404-2005) following Macosko et al ...
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bioRxiv - Cancer Biology 2022Quote: ... 50.000 MDA-MB-231 cells infected with shControl (C) or shLOXL2 (KD) were collected and treated with transposase Tn5 (Nextera Tn5 Transposase, Illumina Cat #FC-121-1030). DNA was purified using AMPure XP beads to remove big fragments (0.5x beads ...
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bioRxiv - Cell Biology 2019Quote: ... Libraries were barcoded and sequenced on a NextSeq Series Sequencing System (HUJI Center for Genomic Technologies) using Illumina flow cell (Illumina 500 NextSeq High Output v2 Kit, FC-404-2005; Illumina, San Diego, CA, USA). The sequencing data were uploaded to the usegalaxy.org (Afgan et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... Sequencing was performed using the NextSeq 500/550 High-Output v2 (150 cycle) Kit (# FC-404-2002) on the NextSeq 550 platform (Illumina Inc, #SY-415-1002). Libraries were combined in an equimolar pool based on the library quantification results and run across 5 High-Output Flow Cell v2.5.
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bioRxiv - Genomics 2021Quote: ... Illumina Catalogue FC-404-2003) following the Illumina recommended denaturation and loading recommendations which included a 1% PhiX spike (PhiX Control v3 Illumina Catalogue FC-110-3001).
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bioRxiv - Cell Biology 2022Quote: ... an average of 500 pg of amplified cDNA was used to prepare the libraries following Nextera XT DNA kit (Illumina Ref. FC-131-1096). Libraries were multiplexed by 6 on a high-output flow cells ...
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bioRxiv - Genomics 2022Quote: ... Illumina Catalogue FC-404-2003) following the Illumina recommended denaturation and loading recommendations which included a 1 % PhiX spike in (PhiX Control v3 Illumina Catalogue FC-110-3001). Data was uploaded to Basespace (www.basespace.illumina.com ...
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bioRxiv - Microbiology 2023Quote: ... 5 µl of this reaction was used in a subsequent round of amplification using BC amp set 3 primers to add Illumina adapters to the sequences followed by amplification using the Nextera XT DNA Library Preparation Kit (Illumina cat. FC-131-1096) to add 5’ and 3’ indices to the library ...
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bioRxiv - Systems Biology 2022Quote: ... single reads and 7 bases index read on an Illumina HiSeq300 instrument using the TruSeq SBS Kit HS-v3 (50-cycle) (FC-401-3002, Illumina Inc, San Diego, CA).
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bioRxiv - Evolutionary Biology 2022Quote: ... sequencing library with a peak insert size of 535 bp was constructed using 200 ng of genomic DNA with a TruSeq Nano LT library preparation kit (Cat # FC-121-4002, Illumina, San Diego, CA, USA) according to the kit supplier’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... library with a peak span size of 1450 bp and a mean span size of 1027 bp was prepared from 570 ng of genomic DNA by tagmentation using an MP library preparation kit (Cat # FC-132-1001, Illumina, San Diego, CA, USA), without size selection ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA samples were then diluted to 0.2-0.3 ng per uL and 1 ng of cDNA was carried over into library preparation using the Nextera XT DNA Library Preparation Kit (Illumina, cat. # FC-131-1096) following the manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: ... A second round of PCR was performed with 50 ng DNA input for 8 cycles to attach Nextera XT indices and adapters using the Illumina Nextera XT Index Kit (Illumina, Catalog #FC-131-200x) and Phusion HF PCR master mix (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: 0.5×105 MIA PaCa-2 cells for each treated condition/replicate infected was collected and treated with transposase Tn5 (Nextera Tn5 Transposase; Illumina Cat #FC-121-1030). DNA was purified using AMPure XP beads to remove large fragments (0.5 × beads ...
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bioRxiv - Immunology 2023Quote: ... The library was then denatured and diluted to 1.2 pM according to standard Illumina’s protocols (for 4 nM libraries) and was spiked with 22% of 20 pM PhiX control (Illumina, cat. no: FC-110-3001). The 1.2 pM library was sequenced on the NextSeq using the NextSeq 500/550 Mid Output Kit v2.5 (300 Cycles ...
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bioRxiv - Immunology 2023Quote: ... Quantified library pool was diluted to 1nM and further diluted as per protocol and sequenced on Illumina MiniSeq (Illumina, Cat. No. FC-420-1001) to check for quality of reads ...
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bioRxiv - Developmental Biology 2023Quote: ... ATAC-seq libraries were generated as described previously (Buenrostro, Giresi et al. 2013) using the Nextera XT DNA Library preparation kit (Illumina catalog: FC-131-1024). Sequencing was performed on an Illumina HiSeq 2500 with 30 - 40 million 75bp paired-end reads ...
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bioRxiv - Plant Biology 2024Quote: ... RNA-sequencing (RNA-seq) libraries for triple replicates of both Col-0 and mac3a/mac3b were prepared utilizing the Illumina TruSeq toolkit (Illumina, Cat# FC-122-1001). Paired-end reads were processed with Trimmomatic (Bolger et al. ...
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bioRxiv - Microbiology 2023Quote: ... Libraries prepared by targeted amplification of the variable V3-V4 regions of the bacterial 16S rRNA gene and attachment of Nextera XT indexes (Illumina Catalog #FC-131-2001) with PrimeStar Taq DNA Polymerase (Takara ...
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bioRxiv - Cell Biology 2024Quote: ... 8 bases for index 1 and 8 bases for index 2) on NextSeq 500 using the NextSeq 500 High Output Kit 75-cycles (Illumina, Cat# FC-404-1005). Flow lanes were loaded at 1.8pM ...
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bioRxiv - Cell Biology 2024Quote: ... 8 bases for index 1 and 8 bases for index 2) on NextSeq 500 using the NextSeq 500 High Output Kit 75-cycles (Illumina, Cat# FC-404-1005). Flow lanes were loaded at 1.8pM ...
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bioRxiv - Genetics 2023Quote: ... The mm10 mouse genome manifest from Illumina was used as reference ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The good quality samples were used to prepare gDNA libraries for all four pooled samples with TruSeq® Nano DNA Library Prep from Illumina (Catalog#FC-121-9010DOC). The libraries were further used for paired-end sequencing with HiSeq2000 (Illumina ...
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bioRxiv - Genomics 2020Quote: ... We run the final pooled library at a concentration of 1.8 pM on a mid-output flow cell (NSQ® 500 Mid Output KT v2 300 cycles; Illumina Catalogue No. FC-404-2003) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... pooled and sequenced with 75 basepair single reads to a depth ranging from 13 to 55 million reads per sample on a Illumina NextSeq 500 using a High Output 75 cycles kit (Illumina, catalog number: FC-404-2005).
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bioRxiv - Genomics 2019Quote: ... We constructed a genomic DNA library using a modified version of the Nextera DNA Library Prep kit (#FC-121-1031, Illumina, Inc., San Diego, CA, USA) meant to conserve reagents [71] ...