Labshake search
Citations for Illumina :
501 - 550 of 1305 citations for PCR Buffers since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... We prepared a genomic DNA library using the TruSeq DNA PCR-free library kit (Illumina) following the manufacturer’s protocol for 550 bp insert libraries ...
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bioRxiv - Microbiology 2019Quote: ... Amplicon libraries were generated using TruSeq® DNA PCR-Free Sample Preparation Kit (Illumina, USA) following the manufacturer’s recommendations and index codes were added ...
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bioRxiv - Microbiology 2019Quote: ... An indexing PCR was next carried out using the Nextera XT Index Kit (Illumina, USA). The index PCR cycle consisted of an initial denaturation step at 95°C for 3 minutes ...
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bioRxiv - Microbiology 2020Quote: ... A single round of PCR was performed using “fusion primers” (Illumina adaptors + indices + specific regions) targeting either the 16S V4-V5 (Bacteria ...
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bioRxiv - Immunology 2020Quote: ... A second PCR was performed with Nextera® XT Index Kit v2 Set A (Illumina) to complete the adapter and add a unique sample-specific barcodes ...
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bioRxiv - Genomics 2021Quote: ... Samples were amplified for 12 cycles of PCR with TruSeq RNA CD Index Plate (Illumina) and pooled ...
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bioRxiv - Molecular Biology 2021Quote: ... 12 cycles were used for Tn5 Nextera PCR amplification (Illumina Nextera DNA UD Indexes Kit). We aimed for 100M read pairs for each run on a HiSeq 2500 sequencer (Illumina) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... An initial multiplex PCR containing locus-specific primers with Illumina (Illumina, Inc., San Diego, CA) priming sites for 229 amplicons was performed ...
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bioRxiv - Genomics 2022Quote: ... 5μL PCR Primer Cocktail from the Illumina TruSeq Nano DNA LT Kit (Illumina, Cat#: 20015964) and 25μL sample ...
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bioRxiv - Plant Biology 2022Quote: ... library construction was performed using TruSeq DNA PCR-Free Library Prep Kit (Illumina, CA, USA). These two libraries were sequenced using the Illumina NextSeq ...
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bioRxiv - Cancer Biology 2022Quote: ... PCR was performed as previously described and samples were sequenced on a MiSeq sequencer (Illumina) (Doench et al. ...
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bioRxiv - Microbiology 2023Quote: ... 1 U PCRBIO HiFi Polymerase (PCR Biosystems) and 10 µL of Nextera adaptor mix (Illumina). PCR conditions were 95°C ...
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bioRxiv - Genomics 2023Quote: ... nine-cycles of PCR were performed using indexed primers from a Nextera Index kit (Illumina), and amplified DNA was size-selected to a size of <500 bp using AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Cancer Biology 2022Quote: ... The final PCR products were subjected to sequencing using NovaSeq system (Illumina, San Diego, CA).
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bioRxiv - Microbiology 2023Quote: ... libraries were generated using the Illumina TruSeq DNA PCR-Free Library Preparation Kit (Illumina, USA), and index codes were added ...
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bioRxiv - Molecular Biology 2023Quote: ... The final PCR products were submitted for next generation sequencing using NovaSeq SP PE250 (Illumina).
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bioRxiv - Molecular Biology 2023Quote: ... DNA fragments were then amplified by PCR using Nextera index primer 1 and 2 (Illumina) and produced by PCR amplification (10–13 cycles ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were generated using TruSeq® DNA PCR-Free Sample Preparation Kit (Illumina, USA) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were quantified by quantitative PCR (qPCR) (KAPA Biosystems Library Quantification Kit for Illumina platforms) and the sequencing was performed on NextSeq500 (Illumina ...
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bioRxiv - Neuroscience 2023Quote: ... An initial PCR amplification was performed on the tagmented DNA using Nextera indexing primers (Illumina). Real-time (RT)-qPCR was run with a fraction of the tagmented DNA to determine the number of additional PCR cycles needed ...
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bioRxiv - Microbiology 2023Quote: Sequencing libraries were generated using the TruSeq DNA PCR-Free Sample Preparation Kit (Illumina, USA), following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were enriched for fragments >200 bp and sequenced on a NextSeq2000 instrument (Illumina) using v2 chemistry ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA from transposed nuclei was then isolated and PCR-amplified using barcoded Nextera primers (Illumina). Library QC was carried out using high sensitivity DNA bioanalyzer assay and Qubit measurement and sequenced using paired-end sequencing (PE50 ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR reactions were performed using primers from Nextera XT Index Kit (Illumina, FC-131-1001) and NEBNext High Fidelity 2X PCR Master Mix (NEB ...
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bioRxiv - Neuroscience 2020Quote: ... and tagmented using the enzyme and buffer provided in the Nextera Library Prep Kit (Illumina). Tagmented DNA was then purified using the MinElute PCR purification kit (Qiagen) ...
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bioRxiv - Biochemistry 2020Quote: ... 1X TD buffer and 0.5μL TDE1 enzyme from the Nextera DNA Sample Prep Kit (Illumina). Tagmentation reactions were cleaned up using a Nucleospin column and eluted in 15μL elution buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... diluted with hybridization buffer and heat denatured before loading on the MiSeq reagent cartridge (Illumina) and on the MiSeq instrument (Illumina) ...
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bioRxiv - Molecular Biology 2022Quote: ... Nuclei pellets were resuspended gently in transposition reaction mix (25 µL 2X TD Buffer [Illumina Cat #FC-121-1030] ...
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bioRxiv - Developmental Biology 2022Quote: ... The samples were then resuspended in 1 ml of ATAC mix (2X TDE buffer (Illumina), 50 µl TDE (Illumina) ...
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bioRxiv - Genomics 2019Quote: ... 12.5 μl of TD buffer (Nextera DNA Library Prep Kit, 15028212, Illumina, San Diego, USA) and 10.5 μl DEPC-treated water ...
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The transcription factor reservoir and chromatin landscape in activated plasmacytoid dendritic cellsbioRxiv - Molecular Biology 2021Quote: ... and tagmented using the enzyme and buffer provided in the Nextera Library Prep Kit (Illumina). Tagmented DNA was then purified using the MinElute PCR purification kit (Qiagen) ...
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bioRxiv - Genomics 2021Quote: ... beads were resuspended in 25 μL Nextera Tagment DNA Buffer (2X) (Illumina FC-121-1030), 3.25 μL TDE1 per 50 ng DNA ...
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bioRxiv - Genomics 2021Quote: ... The pellet from lysed was transposed with Tn5 transposase in 2X tagmentation buffer (Illumina kit) and incubated for 30 min at 37 C ...
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bioRxiv - Immunology 2020Quote: ... and nuclei were resuspended in 50μl of transposition reaction mix (TD buffer [25μl], Tn5 Transposase [2.5μl], nuclease-free water [22.5μl]; (Illumina)) and incubated for 30min at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... and tagmented using the enzyme and buffer provided in the Nextera Library Prep Kit (Illumina). Tagmented DNA was then purified using the MinElute PCR purification kit (Qiagen) ...
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bioRxiv - Developmental Biology 2021Quote: ... and tagmented using the enzyme and buffer provided in the Nextera Library Prep Kit (Illumina). Tagmented DNA was then purified using the MinElute PCR purification kit (Qiagen) ...
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bioRxiv - Genomics 2021Quote: ... Transposition reaction mix containing transposition buffer and 2.5 ul of Tn5 enzyme per sample (Illumina) was added to the pellet and incubated for 30 min at 37 C ...
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bioRxiv - Molecular Biology 2022Quote: ... The transposition reaction mix (25 μl 2X TD buffer, 2.5 μl TDE1 Nextera transposase (Illumina), 16.5 μl PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... and tagmented using the enzyme and buffer provided in the Nextera Library Prep Kit (Illumina). Tagmented DNA was then purified using the MinElute PCR purification kit (Qiagen) ...
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bioRxiv - Cell Biology 2022Quote: ... The nuclei were isolated and transposed with Tn5 transposase in 2X tagmentation buffer (Illumina, #20034197) and incubated at 37 °C on thermomixer for 30 min at 1,000 rpm ...
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bioRxiv - Immunology 2022Quote: ... and nuclei were resuspended in 50μl of transposition reaction mix (TD buffer [25μl], Tn5 Transposase [2.5μl], nuclease-free water [22.5μl]; (Illumina)) and incubated for 30min at 37°C ...
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bioRxiv - Immunology 2022Quote: ... then resuspended with 50 μl of transposase mixture (25 μL of 2x TD buffer (Illumina), 2.5 μL of TDE1 (Illumina) ...
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bioRxiv - Genomics 2023Quote: ... and pellets were resuspended in the transposition reaction mix (25 µL 2X TD buffer (Illumina), 2.5 µL TDE1 Tn5 transposase (Illumina) ...
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bioRxiv - Immunology 2023Quote: ... and tagmented using the enzyme and buffer provided in the Nextera Library Prep Kit (Illumina). Tagmented DNA was purified using the MinElute PCR purification kit (Qiagen) ...
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bioRxiv - Genomics 2023Quote: ... The nuclei were resuspended with 30 μl of loading buffer containing 1x TB1 (Illumina Inc.), 1x standard storage buffer (Illumina Inc.) ...
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bioRxiv - Genetics 2023Quote: ... 700 ng of diluted RNA was mixed with 8.5 uL of EPH buffer (Illumina #20020599) and 1 uL of FastSelect rRNA-depletion enzyme (Qiagen #335377) ...
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bioRxiv - Genomics 2023Quote: ... Frozen pellets were resuspended in 400µl 1X Mammalian Polysome buffer (Illumina TruSeq Ribo Profile (mammalian) kit ...
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bioRxiv - Cancer Biology 2023Quote: ... and tagmented using the enzyme and buffer provided in the Nextera Library Prep Kit (Illumina). Tagmented DNA was then purified using the MinElute PCR purification kit (Qiagen) ...
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bioRxiv - Plant Biology 2024Quote: ... and second strand synthesis buffer (containing dNTPs, RNase H and E. coli polymerase I, Illumina) was added so that the second strand was produced by nick-translation ...
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bioRxiv - Developmental Biology 2024Quote: ... and nuclei were resuspended in 50 mL of reaction buffer (TDE1 transposase 2.5 mL (Illumina), TDE buffer 25 mL (Illumina)) ...