Labshake search
Citations for Illumina :
351 - 400 of 596 citations for Leptospira Biflexa Antigen Strain Patoc 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... RNA-Seq libraries were generated from 1 μg of total RNA from duplicated samples per condition using the TruSeq LT RNA Library Preparation Kit v2 (Illumina) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... Libraries were sequenced by NextSeq 550 High Output with 21 bp for read 1 and 72 bp for read 2 (Illumina). Sequenced reads were aligned using STAR (v2.7.6a ...
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bioRxiv - Genetics 2021Quote: ... The scRNAseq samples were then sequenced on 1/7th of a single S4 lane on a NovaSeq 6000 platform (Illumina). For the ScISOr-Seq library ...
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bioRxiv - Microbiology 2020Quote: ... pooled with 1% denatured PhiX DNA (sequencing control) and sequenced in a MiSeq platform (2× 251 cycles paired-end run; Illumina). New PCR reactions using combinations of the primers described above were carried out to cover regions with low coverage for each sample ...
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bioRxiv - Microbiology 2019Quote: Two overlapping IN-PCR regions corresponding to the 288 amino acids of HIV-1 IN were sequenced with the MiSeq NGS platform (Illumina). The amplicons were purified with Agencourt AMPure XP (Beckman Coulter ...
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bioRxiv - Microbiology 2019Quote: Total RNA (1 µg) from hASCs was used to construct sequencing libraries with the Truseq Stranded Total RNA LT Kit (Illumina). Quality of RNA and constructed libraries was determined via 2100 Bioanalyzer ...
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bioRxiv - Developmental Biology 2021Quote: ... 10%(v/v) N,N-dimethyl formamide) and 1 μl of Tagment DNA Enzyme from Nextera Sample Preparation Kit (Illumina) were added to the DNA-beads complex and incubated for 70 sec at 37 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... Libraries were generated using Kapa Biosystems library preparation kit (#KK8201) and multiplexed libraries were sequenced on a 1×75 High output flow cell on the NextSeq550 platform (Illumina). Reads were filtered and trimmed to remove adapter-derived or low-quality bases using Trimmomatic and checked again with FASTQC ...
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bioRxiv - Genomics 2020Quote: ... Other libraries were sequenced on NextSeq 500 (1x 28 / 1×91 cycles plus 8 base index cycle) using the v2 150 cycle High Output kit (Illumina) as per the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: The accuracy of the variant calls in the filtered dataset was evaluated in two ways: 1) the concordance of genotype calls by Illumina sequencing and by TaqMan allelic discrimination assays (described in Marini et al. ...
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bioRxiv - Genomics 2019Quote: Libraries were prepared using 1 µg of total RNA in the randomized order using TruSeq RNA Sample Prep Kit v2 (Illumina) to generate cDNA as per manufacturer’s instructions using adapters from both Box A and Box B ...
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bioRxiv - Cell Biology 2019Quote: A minimum of 1 µg total RNA in 50 µl was used for library preparation using the TruSeq RNA sample preparation kit (Illumina) following the manufacturer’s protocol at Novogene ...
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bioRxiv - Genetics 2020Quote: ChIP-seq and DNase-seq libraries were prepared from 1-5 ng ChIP DNA or DNase DNA samples using NEBNext Ultra II DNA library Prep Kit (Illumina). Libraries were sequenced on an Illumina NextSeq 500 with single-end or paired-end 75 bp reads.
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bioRxiv - Neuroscience 2019Quote: ... We pooled and sequenced the libraries with paired-end reads (50 bases for read 1 and 25 bases for read 2) on two flowcells with a NextSeq 500 instrument (Illumina).
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bioRxiv - Genomics 2019Quote: ... two different strategies were implemented in order to help in genome scaffolding: 1) Nextera Mate-Pair Preparation Kit (Illumina Inc) was used to make two mate pairs (MP ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 µg of input RNA was used for rRNA removal with Ribo-Zero rRNA Removal Kit (Human/Mouse/Rat) (Illumina). Libraries were generated with the NEBNext Ultra Directional RNA Library Prep kit for Illumina (NEB ...
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bioRxiv - Cancer Biology 2020Quote: ... Libraries were prepared from 1 μg of total RNA using a TruSeq Stranded Total RNA Kit with Ribo-Zero Human/Mouse/Rat (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Amounts of 1 ng cDNA were used to prepare NGS sequencing libraries using Nextera XT DNA Library Prep Kit (Illumina), with unique index adaptors for each sample ...
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bioRxiv - Genomics 2019Quote: ... and marker-wise (HWE P value > 1 × 10−6, call rate > 95%, and for the GSA array additionally by Illumina GenomeStudio GenTrain score > 0.6 ...
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bioRxiv - Systems Biology 2020Quote: ... then performed paired-end sequencing of these libraries (38 cycles read 1 + 37 cycles read 2) on a NextSeq 500 (Illumina). After sequencing ...
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bioRxiv - Immunology 2020Quote: ... cDNA libraries were prepared from 1 μg of total RNA using the TruSeq Stranded mRNA kit (Illumina, San Diego, CA) and the Sciclone NGSx Workstation (Perkin Elmer ...
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bioRxiv - Genomics 2021Quote: ... Following immunoprecipitation with Dynabeads Protein G beads (invitrogen) in PCR tubes, samples were subject to Tagmentation (5 µl Tagmentation Buffer, 1 µl Tagmentation DNA Enzyme (Illumina), 19 µl Nuclease free water ...
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bioRxiv - Genetics 2019Quote: DNA sequencing libraries were constructed from 1 µg of genomic DNA using TruSeq DNA PCR-free Library Prep kit (Illumina). Sequencing was run on an Illumina HiSeq 2500 apparatus using a paired-end read length of 2×150 pb with the Illumina Reagent Kits as already described (Demars et al ...
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bioRxiv - Microbiology 2021Quote: ... Final sequencing pools were loaded at 12pM concentration onto an Illumina MiSeq 300 cycle sequencing run with a 1% PhiX spike-in (Illumina).
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bioRxiv - Cancer Biology 2019Quote: WGS libraies were generated from 1 μg of 50 ng/ul high molecular weight gDNA using the TruSeq PCR-free Library Prep Kit (Illumina), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Libraries were characterized using an Agilent Technologies 2100 Bioanalyzer and quantified before sequencing using NextSeq500 and 1 x 75 b read chemistry (Illumina).
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bioRxiv - Cell Biology 2021Quote: ... 8 bases for index 1 and 8 bases for index 2) using the NextSeq 500 High Output Kit 75-cycles (#FC-404-1005, Illumina) loaded at 1.8pM and including 1 % PhiX ...
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bioRxiv - Systems Biology 2021Quote: ... GBC sequencing libraries were spiked into transcriptome libraries at 1:10 and sequenced on the NextSeq or MiSeq platform (Illumina). Transcriptome libraries were sequenced separately using a NovaSeq5000 S2 300-cycle kit.
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bioRxiv - Systems Biology 2021Quote: ... Samples representative of all treatment groups were multiplexed and sequenced on an Illumina NextSeq500 instrument using high-output 1×75-bp geometry (Illumina).
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bioRxiv - Systems Biology 2020Quote: ... Sequencing was performed using custom read primer oligo1210 (5’-CTTGTGGAAAGGACGAAACACCGGTAATTTCTACTCTTGTAGAT) (HPLC purified, Integrated DNA Technologies) using NextSeq 1 × 75 nt High Output reagents (Illumina).
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bioRxiv - Microbiology 2021Quote: ... The libraries were either paired-end sequenced using 2×75 bp read length (Replica R2 samples) or single-end sequenced using 50 bp read length (Replica 1 samples) on an NextSeq 500 system (Illumina). RNA-seq analysis was performed according to (76) ...
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bioRxiv - Plant Biology 2020Quote: ... and equally pooled in 1 lane of single end 50 bp sequencing in HiSeq 2000 machine (Illumina, San Diego, CA) at the QB3 facility at UC Berkeley.
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bioRxiv - Genomics 2022Quote: Library preparation from 1-10ng rRNA-depleted RNA was performed using NEBNext UltraII Directional RNA Library Preparation Kit (Illumina, #E7760) following the vendor protocols and complementing cleaning steps with NEBNext Sample Purification Beads (#E7767) ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by library construction using non-stranded (Replicate 1) or stranded (Replicates 2 and 3) TruSeq mRNA Library Prep Kit (Illumina). The resulting libraries were sequenced on Hiseq4000.
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bioRxiv - Neuroscience 2022Quote: ... Libraries were sequenced on a HiSeq 2000 and/or HiSeq2500 on a 1×50 bp single read (SR) flow cell (Illumina) at an average of 20M reads/sample ...
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bioRxiv - Cancer Biology 2022Quote: ... Libraries were generated from 1 μg of total RNA using a TruSeq Stranded Total RNA Library Prep Human/Mouse/Rat (48 Samples) (Illumina) per manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... including larger than expected correlations between samples with certain barcode combinations (Supplementary Note 1) and a small but significant number of reads mapping to impossible barcode combinations (combinations of CEL-seq and Illumina barcodes that were not used in the experiment) ...
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bioRxiv - Immunology 2020Quote: ... Use of 250 pg of cDNA with 1/5 reaction of Illumina Nextera XT kit (Illumina, San Diego, CA, USA). The length distribution of the cDNA libraries was monitored using DNA High Sensitivity Reagent Kit on the Perkin Elmer Labchip GX system (PerkinElmer ...
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bioRxiv - Microbiology 2020Quote: ... 4-5 subsamples per replicate were pooled in equimolar concentrations and sequenced on a HiSeq2500 in a 1×50 bp Rapid Run (Illumina). As the first sequencing run did not deliver sufficient data for seven metatranscriptomes ...
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bioRxiv - Developmental Biology 2021Quote: ... Libraries were generated using Kapa Biosystems library preparation kit (#KK8201) and multiplexed libraries were sequenced on a 1×75 flow cell on the HiSeq2000 platform (Illumina). Reads were filtered and trimmed to remove adapter-derived or low-quality bases using Trimmomatic and checked again with FASTQC ...
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bioRxiv - Evolutionary Biology 2021Quote: ... A paired-end Illumina library (DRR029460; see also Supplementary File 1) was constructed using a TruSeq Stranded mRNA LT Sample Prep Kit (Illumina) and an RNA sample extracted from an E ...
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bioRxiv - Immunology 2022Quote: ... TCRseq libraries were sequenced on an Illumina Novaseq 6000 SP flowcell (67 bp read 1 and 459 bp read 2) (Illumina) to a depth of approximately 20,000 reads.
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bioRxiv - Immunology 2022Quote: ... Targeted RNAseq and Sample Tag libraries were sequenced on an Illumina Novaseq 6000 S4 flowcell (67 bp read 1 and 140 bp read 2) (Illumina) to a depth of approximately 40,000 reads and 10,000 reads per cell ...
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bioRxiv - Immunology 2022Quote: ... whose lengths were about 600 base pairs were sequenced using an Illumina Novaseq 6000 S4 flowcell (67 bp read 1 and 140 bp read 2) (Illumina). Only read2 contained the sequence regarding the definition of T cell clones.
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bioRxiv - Immunology 2022Quote: ... Small RNA libraries were prepared using 1 µg of total RNA and the TruSeq Small RNA Sample Prep Kits (Illumina) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... then single-end sequenced (1 x 100 bp) on one lane using TrueSeq PE150 kit (Illumina, Inc., San Diego, CA) on an Illumina HiSeq 2000 instrument ...
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bioRxiv - Molecular Biology 2022Quote: The following publicly available datasets were downloaded and used for comparison to STORM-seq: 1) K562 SMART-seq2 (Illumina runs) - SRP132313 ...
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bioRxiv - Cancer Biology 2020Quote: Sequencing was performed by the Human & Environmental Genomics core facility of Rennes on a HiSeq 1500 (Rapid SBS kit v2 1×100 cycles, Illumina). Base Calling was performed with Illumina’s CASAVA pipeline (Version 1.8).
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bioRxiv - Genomics 2019Quote: ... 5 mM MgCl2) containing 1 μl Tn5 transposase from the Nextera DNA Library Prep Kit (15028212, Illumina, San Diego, USA) and incubated at 37°C for 10min ...
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bioRxiv - Cancer Biology 2019Quote: ... Sequencing libraries were generated by the UNMC NGS Core beginning with 1 µg of total RNA from each sample using the TruSeq V2 RNA sequencing library kit from Illumina following recommended procedures (Illumina Inc. ...