Labshake search
Citations for Illumina :
351 - 400 of 403 citations for tert Butyl 10 oxo 4 9 diazaspiro 4.5 decane 4 carboxylate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2024Quote: ... 15 µL of cleaned-up PCR product from the first PCR and 10 µL Nextera DNA CD Indexes (96-well format from Illumina) for dual indexing ...
-
bioRxiv - Cancer Biology 2024Quote: Dissociated cells were cryopreserved in 10% dimethyl sulfoxide in DMEM containing 10% FBS until processing on the 10X Genomics’ Chromium Single Cell platform and sequencing on a NovaSeq 6000 System (Illumina) at a depth of approximately 400 million reads per sample ...
-
bioRxiv - Cancer Biology 2024Quote: ... Sequencing libraries were constructed according to the manufacturer’s instructions (10× Genomics) and sequenced on a NovaSeq 6000 system (Illumina, 20012866) (147).
-
bioRxiv - Cell Biology 2024Quote: ... The cDNA was then amplified using 10 cycles and subsequently used to construct a pool of uniquely indexed samples using the Nextera XT kit (Illumina). Finally ...
-
bioRxiv - Cell Biology 2020Quote: ... Libraries were then indexed and PCR amplified (10 cycles) and sequenced on Illumina HiSeq 2500 sequencing platform following the manufacture’s protocols (Illumina, Hayward CA.).Sequence reads were aligned to GRCh37-lite using Burrows-Wheeler Aligner (BWA ...
-
bioRxiv - Neuroscience 2020Quote: ... The cluster was generated by cBot with the library diluted to 10 pM and then was sequenced on the Illumina NovaSeq 6000 (Illumina, USA). The library construction and sequencing were performed by Shanghai Sinotech Genomics Corporation (China).
-
bioRxiv - Immunology 2020Quote: ... Supernatant was discarded and nuclei were re-suspended in 50 μl reaction buffer containing 5.0 μl Tn5 transposase and 10 μl of 5 × TTBL buffer (TruePrepTM DNA Library Prep Kit V2 for Illumina, Vazyme Biotech). The reaction was incubated at 37°C for 30 min ...
-
Induction of Dopaminergic Neurons for Neuronal Subtype-Specific Modeling of Psychiatric Disease RiskbioRxiv - Neuroscience 2021Quote: ... RNA fragmentation was performed at 94°C for 6 minutes and 10 PCR cycles were used during library amplification with TruSeq single-index adapters (Illumina, #20020492). Final library concentrations were quantified with both Qubit fluorometric quantification (DNA dsDNA HS kit ...
-
bioRxiv - Cancer Biology 2022Quote: ... and sequenced on a total of 10 Hiseq 2500 lanes using the 125bp paired-end sequencing method (Illumina, San Diego, CA). Both reads of each sample were trimmed for adapters and low-quality bases using Trimmomatic software and aligned with reference human hg19 genome and ensemble v70 transcripts using Tophat software as stranded libraries ...
-
bioRxiv - Developmental Biology 2022Quote: ... After centrifugation supernatant was used for RNA cytosolic fraction by adding to 1 ml of TRIZOL and isolated nuclei of CMs were incubated in 30 µl and of EC in 10 µl transposase mixture with 5% Nextera Tagment DNA Enzyme TDE (Illumina #15027916) in transposition buffer (20 mM Tris-HCl pH 7.6 ...
-
bioRxiv - Synthetic Biology 2022Quote: ... Sequencing library preparation was then performed using Stranded Total RNA Prep Ligation kit with Ribo-Zero Plus for rRNA depletion and by using 10 bp IDT for Illumina indices (all from Illumina, USA). Sequencing was done on a NextSeq2000 instrument in 2×50 bp paired-end mode ...
-
bioRxiv - Molecular Biology 2024Quote: ... Libraries were then indexed and PCR amplified (10 cycles) and sequenced on Illumina HiSeq 2500 sequencing platform following the manufacture’s protocols (Illumina, Hayward CA).
-
bioRxiv - Microbiology 2023Quote: ... Library preparation was performed using the Illumina DNA Prep Kit and IDT 10 bp UDI indices (Illumina, San Diego, CA, USA), hybrid whole genome sequencing was carried out on an Illumina NextSeq 2000 (2 × 151 bp reads ...
-
bioRxiv - Microbiology 2023Quote: ... Samples were equimolarly pooled with 10% PhiX control DNA and sequenced on an Illumina MiSeq using a MiSeq 2 × 250 v2 kit (Illumina, Inc.). Due to the number of samples ...
-
bioRxiv - Biophysics 2023Quote: ... 100-120 μl pooled-denatured 20 pM library and 10-20 μl 20 pM PhiX control library (Illumina, FC-110-3001) to provide a reasonable spot density in general ...
-
bioRxiv - Cell Biology 2024Quote: ... The libraries were sequenced (single read, 1x50 cycles) at a concentration of 10 pm/lane on a HiSeq 2500 (Illumina Inc.).
-
bioRxiv - Genetics 2024Quote: ... DNA was submitted to the Mammalian Genotyping Core at University of North Carolina to be genotyped using the Infinium Global Diversity Array-8 v.10 Kit (Illumina #20031669).
-
bioRxiv - Genomics 2024Quote: ... ChIP-seq libraries were prepared from 10 ng of immunoprecipitated DNA using TruSeq ChIP Library Preparation Kit (Illumina, IP-202-1012) according to the manufacturer’s instructions with minor modifications ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... We performed whole-genome Illumina library preparations and sequencing to ∼10× depth using 150-bp paired-end reads generated on the NovaSeq (Illumina Inc.) platform at Duke University ...
-
bioRxiv - Genomics 2020Quote: ... Five size-fractions were selected ranging from 470 bp to 10 kb to construct sequencing libraries following the manufacturer’s protocols (Illumina, San Diego, CA). The TruSeq DNA Sample Preparation Kit version 2 with no PCR amplification (PCR-free ...
-
bioRxiv - Neuroscience 2020Quote: ... and 40 from each of the 10 donors) were prepared with the TruSeq Stranded mRNA kit from Illumina (San Diego, CA, USA) and sequenced on a HiSeq 4000 sequencer (Illumina ...
-
bioRxiv - Developmental Biology 2022Quote: ... The library samples were then diluted to a 10 nM concentration and sequenced on an Illumina NovaSeq 6000 platform (Illumina, San Diego) for PE150 sequencing by Annoroad Gene Tech ...
-
bioRxiv - Cancer Biology 2020Quote: ... Drop-seq denatured libraries were loaded at 1.3pM final concentration and were spiked in with 10% of 1.8pM PhiX Control v3 (Illumina Cat# FC-110-3001). Sequencing specifications were as follows ...
-
bioRxiv - Developmental Biology 2020Quote: ... RNA was extracted from five biological replicates of each of the 222 and ii2 diet groups (10 samples in total) using the TruSeq Stranded mRNA-seq kit (Illumina, DA, USA). The samples were sequenced in one flowcell on the NextSeq 500 producing paired end reads of 75bp.
-
bioRxiv - Genomics 2020Quote: ... using 5% PhiX without dark cycles (n = 11) or 10-20% PhiX with 7 dark cycles (protocol provided by Illumina, n = 49). A maximum of 12 samples were pooled in one sequencing run resulting in 19.05 million [17.05 – 21.72] single-end reads per sample on average (supplementary table 3).
-
bioRxiv - Genetics 2024Quote: ... 10 ng of amplicon DNA from the first PCR reaction was combined with 10 µL of Nextera adapter index primers (Illumina Cat#20027213) and 25 µL Kapa HiFi Hotstart ReadyMix ...
-
bioRxiv - Microbiology 2024Quote: ... RiboZero: rRNA was removed from 10 ng of total RNA using Ribo-Zero(TM) rRNA Removal Kit (Illumina, San Diego, CA, USA). Stranded cDNA libraries were generated using the Illumina Truseq Stranded mRNA Library Prep kit ...
-
bioRxiv - Microbiology 2021Quote: ... Fragments were then indexed with 10 nucleotide barcode sequences using Nextera XT Index Kit v2 Set D primers (Illumina, San Diego, California, USA). Indexing PCR reactions were run in triplicate with a volume of 25 μL using 12.5 μL NEB Next High-Fidelity Master Mix ...
-
bioRxiv - Molecular Biology 2020Quote: ... The resulting library was diluted to 10-pM in 600-μL of HT1 hybridization buffer (Illumina Nextera XT kit Cat#FC-131-1024) and 10-μL was loaded onto a 300-cycle MiSeq Nano v2 flow cell (Illumina Cat#MS-102-2002 ...
-
bioRxiv - Microbiology 2020Quote: ... The pool was sequenced on an Illumina MiSeq using the 2 × 250 bp v2 kit with a 10% PhiX control following the manufacturer’s protocol (Illumina, Inc., San Diego, CA) and using custom primers developed from (55) ...
-
bioRxiv - Neuroscience 2022Quote: ... Approximately 10 ug of total RNA was used to remove ribosomal RNA using an Epicentre Ribo-Zero Gold Kit (Illumina, San Diego, USA). Linear RNA was digested following standard protocols using Epicentre Ribonuclease R (Illumina ...
-
bioRxiv - Microbiology 2023Quote: ... RNA samples with RQNs ranging from 8 to 10 were used for RNA library preparation with the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA). Briefly ...
-
bioRxiv - Genomics 2023Quote: ... about 1.5 ng purified pre-amplified cDNA sample was fragmented in four 20 μl tagmentation mix (10 μl 2x TD buffer, 5 μl Nextera XT (Illumina, FC-131-1096), and 5 μl cDNA sample ...
-
bioRxiv - Molecular Biology 2023Quote: ... instrument at a concentration of 300 pM with 10% PhiX using the NovaSeq 6000 SP Reagent Kit v1.5 (100 cycles) (Illumina, San Diego, CA, USA) (paired-end ...
-
bioRxiv - Physiology 2023Quote: ... RNA samples with RQNs ranging from 8 to 10 were used for RNA library preparation with the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA). Briefly ...
-
bioRxiv - Neuroscience 2023Quote: ... RNA samples with RQNs ranging from 8 to 10 were used for RNA library preparation with the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA). Briefly ...
-
bioRxiv - Genomics 2024Quote: ... 11 μL Nuclease-free water and 2 μL RP1 and RPIx primers each (10 mM, for details see Illumina smallRNA indexing primer sequences) to the 10 μL single-stranded DNA product ...
-
bioRxiv - Microbiology 2024Quote: ... with up to 500 ng DNA input following the manufacturer’s protocol using unique dual-index 10 bp barcodes with Nextera® adapters (Illumina, San Diego, CA). All libraries were pooled in equal abundance ...
-
bioRxiv - Genetics 2024Quote: ... The TrueMethyl converted DNA samples were then eluted in 10 µL and then processed through the Illumina Infinium MethylationEPIC BeadChip array (Illumina, San Diego, CA) protocol ...
-
bioRxiv - Microbiology 2020Quote: ... The sample was loaded at a concentration of 10 pM and sequenced using 2×250 paired-end strategy on the Miseq (Illumina San Diego, CA, USA) platform for 251 cycles.
-
bioRxiv - Molecular Biology 2022Quote: ... a final dilution to 4nM was performed and 13 pM of the final denaturated library was loaded onto the Illumina MiSeq® with 10% PhiX using 2 × 300 bp v3 chemistry (Illumina Inc., CA, USA).
-
bioRxiv - Genomics 2021Quote: ... and four mate-pair sequencing libraries (insert sizes of 2, 5, 10, and 15 kb) in accordance with manufacturer protocols (Illumina, San Diego, CA, USA). Libraries were then sequenced using a HiSeq2000 instrument (Illumina) ...
-
bioRxiv - Developmental Biology 2021Quote: ... 100-500 ng of total RNA with RIN values of 9.5-10 underwent polyA selection and TruSeq library preparation according to instructions provided by Illumina (TruSeq Stranded mRNA LT Kit), with 8 cycles of PCR ...
-
bioRxiv - Molecular Biology 2020Quote: ... 13 pM library was loaded on the Illumina MiSeq® with 10% PhiX using a 2 × 300 bp V3 chemistry (Illumina Inc., CA, USA).
-
bioRxiv - Evolutionary Biology 2023Quote: ... The libraries for Illumina sequencing were constructed according to standard protocols using the Illumina DNA prep kit and IDT 10 bp UDI indices (Illumina, San Diego, CA, USA) by the sequencing facility at SEQCENTER (PA ...
-
bioRxiv - Molecular Biology 2024Quote: ... 500 ng of total RNA with RIN values of 9.9-10 underwent polyA selection and TruSeq library preparation according to instructions provided by Illumina (TruSeq Stranded mRNA LT Kit), with 8 cycles of PCR ...
-
bioRxiv - Developmental Biology 2024Quote: ... 100-500 ng of total RNA with RIN values of 9.5-10 underwent polyA selection and TruSeq library preparation according to instructions provided by Illumina (TruSeq Stranded mRNA LT Kit), with eight cycles of PCR ...
-
bioRxiv - Cancer Biology 2024Quote: ... and the pooled library was diluted to 10 nM for sequencing on the NextSeq 550 sequencing platform (High output, 75bp single-end, Illumina, San Diego, CA, USA).
-
bioRxiv - Developmental Biology 2024Quote: ... 100-500 ng of total RNA with RIN values of 9.5-10 underwent polyA selection and TruSeq library preparation according to instructions provided by Illumina (TruSeq Stranded mRNA LT Kit), with 8 cycles of PCR ...
-
bioRxiv - Genomics 2020Quote: ... Post-capture DNA libraries were combined into 10 pools of approximately 96 samples each and sequenced on three lanes on an Illumina HiSeq 3000 (Illumina Inc., San Diego, CA, USA) to generate approximately 0.5 million 2x150-bp paired-end reads per sample ...