Labshake search
Citations for Illumina :
351 - 400 of 1305 citations for PCR Buffers since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... 9-cycle PCR were performed using indexed primers from Nextera Index Kit (Illumina) and KAPA HiFi HotStart ReadyMix (KAPA Biosystems) ...
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bioRxiv - Genomics 2022Quote: ... Illumina TruSeq DNA PCR-free Library Preparation Kits (Illumina, San Diego, CA, USA) were used for library sequencing according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... index sequences were added by PCR using Nextera XT index kit (Illumina, US). The amplicons were sent for sequencing with a paired-end read chemistry by a MiSeq sequencer (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... Libraries were cleaned up with the MagBio HighPrep PCR Clean-up System (Illumina), quantified on a CFX96 Touch Real-Time PCR System (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... and submitted to an additional 10 rounds of PCR with indexing primers (Illumina). The resulting libraries were pooled by volume with specimens at 100x the positive controls ...
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bioRxiv - Molecular Biology 2023Quote: ... First-round PCR primers contained adapter sequence for DNA/RNA UD Indexes (Illumina). The second round of PCR and pooling of samples was performed according to the Illumina Nextera DNA library prep guide ...
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bioRxiv - Microbiology 2023Quote: ... Secondary PCR was performed with forward and reverse primer sequences designated by Illumina. The thermal condition was set to be the same as the first PCR but for 8 cycles ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1.25 μM i5 and i7 PCR primers (Nextera® Index Kit (Illumina)) with the following PCR amplification conditions ...
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bioRxiv - Microbiology 2023Quote: ... Final PCR products were gel purified and sequenced using Illumina Miseq sequencer (Illumina). Sequencing reads were quality filtered (Q value ≥20 ...
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bioRxiv - Microbiology 2023Quote: ... Two nanograms of PCR product were used in a two-fifths Nextera (Illumina) library prep with 15 cycles of amplification ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The libraries were validated following the Library Quantitative PCR (qPCR) Quantification Guide (Illumina). Following ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA-seq libraries were prepared using the kit TruSeq DNA PCR-Free (Illumina). Then ...
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bioRxiv - Physiology 2023Quote: Illumina TruSeq DNA PCR-free library preparation (Illumina Cat no: 20015963, Illumina, USA) was used to generate high quality DNA sequencing libraries ...
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bioRxiv - Physiology 2023Quote: Illumina TruSeq DNA PCR-free library preparation (Illumina Cat no: 20015963, Illumina, USA) was used to generate high quality DNA sequencing libraries ...
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bioRxiv - Microbiology 2023Quote: ... Reverse primers contained i7 indexes from the TruSeq DNA PCR Free kit (Illumina). PCR reactions were run on agarose gels to verify amplification ...
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bioRxiv - Cancer Biology 2023Quote: ... The PCR products were then purified and sequenced on a NextSeq 500 (Illumina) (Primer sequences are annotated in Table S5 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... DNA libraries were made using TruSeq DNA PCR-free Library kit from Illumina as per the manufacturer’s instructions and were multiplexed based on concentrations measured with a Qubit Flourometer 2.0 (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... additional PCR amplification was conducted using the Nextera XT Index Kit (15032350, Illumina). The final library was purified once again using AMPure XP beads ...
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bioRxiv - Evolutionary Biology 2024Quote: ... (Seoul, South Korea) using TruSeq DNA PCR-Free Protocol (Illumina, San Diego, CA) and genome sequencing was performed with the Illumina HiSeq 2000 platform in pair-end mode (2×151bp) ...
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bioRxiv - Developmental Biology 2021Quote: ... The nuclei were suspended in a transposition mix (TD Tagment DNA Buffer, Illumina Catalog No ...
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bioRxiv - Neuroscience 2021Quote: ... Pelleted nuclei were resuspended in 25μL Transposition mix (12.5μL 2x TD buffer (Illumina), 1.25μL transposase (100nM final ...
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bioRxiv - Cancer Biology 2021Quote: ... Nuclei were incubated with Tn5 transposon enzyme in transposase reaction mix buffer (Illumina) for 30 min at 37°C ...
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bioRxiv - Plant Biology 2021Quote: ... Tagmentation was performed using the Tagment DNA Enzyme and Buffer kit (Illumina, 20034210). Transposed DNA fragments were purified using AMPure XP beads (Beckman Coulter ...
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bioRxiv - Neuroscience 2021Quote: ... and cell pellet gently resuspended in 20 μ ltagment DNA buffer (Nextera, Illumina), 1 μtagment DNA enzyme (Nextera ...
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bioRxiv - Genomics 2019Quote: ... we combined the purified cDNA with 4 µl of Nextera TD buffer (Illumina) and 1 µl of Nextera Tn5 enzyme (Illumina ...
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bioRxiv - Genomics 2019Quote: ... Each well was then mixed with 5 μL Nextera™ TD buffer (Illumina) and 1 μL i7 only TDE1 enzyme (25 nM ...
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bioRxiv - Immunology 2021Quote: ... combined the following: 25 μl 2x TD Buffer (Illumina Cat #FC-121-1030), 2.5 μl Tn5 Transposase (Illumina Cat ...
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bioRxiv - Developmental Biology 2022Quote: ... 10 µl of 2x TD Buffer (from Nextera XT DNA Prep Kit, Illumina) and 2 µl of Tn5 Transposase (from Nextera XT DNA Prep Kit ...
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bioRxiv - Developmental Biology 2023Quote: ... Supernatant was removed and pellet was resuspended in 47.5 µL buffer TD (Illumina). 2.5 uL of Tagment DNA Enzyme (Illumina ...
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bioRxiv - Developmental Biology 2023Quote: ... combine the following per sample: 25 μl 2x TDE1 Buffer (Illumina Cat #20034197), 3.5 μl Tn5 Enzyme (100nM final ...
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bioRxiv - Cell Biology 2022Quote: ... The nuclei were pelleted and resuspended in Tn5 enzyme and transposase buffer (Illumina Nextera® DNA library preparation kit ...
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bioRxiv - Systems Biology 2023Quote: ... Transposase treatment was performed in 10ul volume containing 1xTD buffer (Nextera kit, Illumina), 0.5ul “home-made” Tn5 transposase adapter complex and 0.4mg/ml digitonin (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... coli transposition assays were mixed with 5 µL of tagmentation DNA buffer (Illumina) and 1 µL of amplicon tagmentation mix (Illumina ...
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bioRxiv - Cancer Biology 2023Quote: ... Each well contained 5□μL NIB and 5□μL TD buffer from Illumina, and 1 mL of 2.5 mM uniquely indexed transposome ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysed cells were then treated with 2xTD reaction buffer (Illumina, #FC-121-1030) and TDE1 Nextera Tn5 Transposase (Illumina ...
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bioRxiv - Cell Biology 2023Quote: ... after which a custom second-strand synthesis buffer (Illumina, San Diego, California, US), dNTPs ...
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bioRxiv - Genomics 2024Quote: ... Libraries were prepared using the tagment DNA Enzyme and Buffer Large Kit (Illumina), and size-selected libraries (300-500 bp ...
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bioRxiv - Immunology 2024Quote: ... ATAC libraries were prepared using the Tagment DNA Enzyme and Buffer Kit (Illumina) following the Omni-ATAC protocol (18) ...
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bioRxiv - Developmental Biology 2021Quote: ... a 9-cycle PCR was performed using indexed primers from Nextera Index Kit (Illumina) and KAPA HiFi HotStart ReadyMix (KAPA Biosystems) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Individual genomic libraries were prepared using TruSeq DNA PCR-free Low Sample Kit (Illumina) and subsequently sequenced (150 bp paired-end ...
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bioRxiv - Biophysics 2022Quote: ... and the library was generated using TruSeq™ DNA PCR-Free kit from Illumina. The sequencing was done on an Illumina HiSeq 2500 250PE platform at Macrogen ...
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bioRxiv - Immunology 2022Quote: Both PCR reactions were combined and cleaned with 1X ratio Sample Purification Beads (Illumina) at a 1:1 bead to sample ratio ...
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bioRxiv - Cell Biology 2019Quote: ... and PCR enriched using the Nextera DNA Library Prep Kit (Illumina, San Diego, CA). Libraries were then pooled in equimolar amounts and purified of smaller fragments using 0.7x Agencourt AMPure XP beads ...
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bioRxiv - Microbiology 2019Quote: Libraries were prepared using a TruSeq DNA PCR-Free Library Preparation Kit (Illumina, USA) and were sequenced on anIllumina-MiSeq system using a 600-cycle v3 reagent kit ...
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Loss of microbial diversity and body site heterogeneity in individuals with Hidradenitis SuppurativabioRxiv - Microbiology 2019Quote: ... tailed PCR approach that has been validated by Illumina (Illumina, Inc, San Diego, CA).
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bioRxiv - Microbiology 2019Quote: ... and appended by PCR using the Nextera XT index kit (Illumina, San Diego, CA). The libraries were further purified using Agencourt AMPure XP Beads ...
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bioRxiv - Microbiology 2019Quote: ... The second round indexing PCR was performed using the Nextera XT DNA protocol (Illumina). Each MiSeq run included three types of negative control to identify potential background contamination from sample manipulation equipment ...
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bioRxiv - Genomics 2020Quote: ... and TruSeq DNA PCR-Free library was prepared following the manufacturers protocol (Illumina Inc.). The normalized library was paired-end sequenced in 2×101 bp ...
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bioRxiv - Genetics 2021Quote: ... A library prepared with TruSeq DNA PCR-Free LT Sample Prep Kit (Illumina, USA) was used for the first run ...
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bioRxiv - Genetics 2020Quote: ... PCR products were analyzed for ultra-deep high throughput sequencing in a NovaSeq (Illumina). The coverage was >10000 reads per sample.