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Citations for Illumina :
301 - 350 of 563 citations for Vitamin K epoxide reductase complex subunit 1 VKORC1 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: The triplicate total RNA samples used for single-end 1×75bp RNA-Seq on a NextSeq500 sequencing platform (Illumina). After examining the data quality using FastQC (v0.11.5) ...
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bioRxiv - Genomics 2022Quote: ... 5 μl of Index primer (both provided in NEBNext Multiplex Oligos for Illumina Index Primers Sets 1 and 2), and 25 μl NEBNext Ultra II Q5 Master Mix (PCR cycling conditions ...
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bioRxiv - Plant Biology 2023Quote: ... The library was diluted to 0.5 nM with EB in 18 μL and spiked with 1% PhiX Control v3 (Illumina) before being prepared for loading using a NovaSeq XP 2 lane kit v1.5 (20043130 ...
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bioRxiv - Immunology 2022Quote: ... 5,000-50.000 cells per experiment were centrifuged 5 minutes at 8°C, and resuspended in 25 µl transposase mixture (12.5 µl Tagmentation DNA buffer, 1 µl Tn5 transposase (Illumina), 10.75 µl nuclease-free water ...
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bioRxiv - Microbiology 2023Quote: ... 1 ng of DNA was used to prepare DNA libraries using the Nextera XT DNA Library Preparation Kit (Illumina) and genomic DNA was fragmented with Illumina Nextera XT fragmentation enzyme ...
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bioRxiv - Developmental Biology 2024Quote: ... The mRNA-Seq libraries were prepared with 1 μg of total RNA using the TruSeq RNA Library Prep Kit v2 per the manufacturer’s instructions (Illumina). Three independent biological replicates were prepared for each condition and libraries were sequenced on an Illumina HiSeq 4000 instrument (single-end ...
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bioRxiv - Genomics 2024Quote: The read 1 fastq file from each spatial region was mapped on the mm10 reference genome (source: Illumina igenomes) using the STAR package (v 2.7.9a ...
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bioRxiv - Cell Biology 2024Quote: ... we prepared the mRNA-Seq libraries using 1 μg of total RNA and the TruSeq RNA Library Prep Kit v2 according to the manufacturer’s instructions (Illumina). The samples were sequenced on an Illumina HiSeq 4000 instrument (single-end ...
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bioRxiv - Genomics 2024Quote: ... We added a FORMAT (GT) and SAMPLE (0/1) column to the VCF and used hap.py (https://github.com/Illumina/hap.py) to compare the v4.2.1 benchmark VCF and BED (https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/ChineseTrio/HG005_NA24631_son/NISTv4.2.1/GRCh38/) ...
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bioRxiv - Molecular Biology 2024Quote: ... The PCR product was purified from a 1% agarose gel and sequenced using a NextSeq 550 or NovaSeq (Illumina) to obtain 79+79-nt or 150+150-nt ...
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bioRxiv - Immunology 2024Quote: ... All cDNA libraries were sequenced on an Illumina NextSeq 500 instrument according to Illumina and 10x Genomics guidelines with 1.4-1.8pM input and 1% PhiX control library spike-in (Illumina, USA).
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bioRxiv - Physiology 2024Quote: ... 10x libraries were pooled and charged with 1% PhiX on one S1 lane of the NovaSeq 6000 instrument (Illumina) using the NovaSeq 6000 S1 Reagent Kit v1.5 (100 cycles) ...
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bioRxiv - Neuroscience 2024Quote: ... 5 ul of Tn5 tagmentation mix (1X Tagment DNA buffer, Illumina; ATM mix, Illumina and cDNA library 1 ng) was prepared for cDNA fragmentation ...
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bioRxiv - Developmental Biology 2024Quote: ... cleaned up by 0.6:1 AMPure XP beads and sequenced on NovaSeq for 150-bp paired end reads (Illumina).
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bioRxiv - Cancer Biology 2024Quote: ... diluted to 650 pM in RSB buffer and mixed with 1% PhiX Control v3 DNA (Illumina FC-110-3001) for sequencing ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA libraries were created from 1 µg DNase-treated RNA with the TruSeq Stranded Total RNA Globin kit (Illumina) and sequenced on the Illumina NovaSeq6000 ...
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bioRxiv - Developmental Biology 2024Quote: ... cleaned up by 0.6:1 AMPure XP beads and sequenced on NovaSeq for 150-bp paired end reads (Illumina).
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bioRxiv - Cell Biology 2020Quote: ... RNA-Seq libraries were generated from 1 μg of total RNA from duplicated samples per condition using the TruSeq LT RNA Library Preparation Kit v2 (Illumina) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... Libraries were sequenced by NextSeq 550 High Output with 21 bp for read 1 and 72 bp for read 2 (Illumina). Sequenced reads were aligned using STAR (v2.7.6a ...
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bioRxiv - Genetics 2021Quote: ... The scRNAseq samples were then sequenced on 1/7th of a single S4 lane on a NovaSeq 6000 platform (Illumina). For the ScISOr-Seq library ...
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bioRxiv - Microbiology 2020Quote: ... pooled with 1% denatured PhiX DNA (sequencing control) and sequenced in a MiSeq platform (2× 251 cycles paired-end run; Illumina). New PCR reactions using combinations of the primers described above were carried out to cover regions with low coverage for each sample ...
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bioRxiv - Developmental Biology 2021Quote: ... 10%(v/v) N,N-dimethyl formamide) and 1 μl of Tagment DNA Enzyme from Nextera Sample Preparation Kit (Illumina) were added to the DNA-beads complex and incubated for 70 sec at 37 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... Libraries were generated using Kapa Biosystems library preparation kit (#KK8201) and multiplexed libraries were sequenced on a 1×75 High output flow cell on the NextSeq550 platform (Illumina). Reads were filtered and trimmed to remove adapter-derived or low-quality bases using Trimmomatic and checked again with FASTQC ...
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bioRxiv - Genomics 2020Quote: ... Other libraries were sequenced on NextSeq 500 (1x 28 / 1×91 cycles plus 8 base index cycle) using the v2 150 cycle High Output kit (Illumina) as per the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ChIP-seq and DNase-seq libraries were prepared from 1-5 ng ChIP DNA or DNase DNA samples using NEBNext Ultra II DNA library Prep Kit (Illumina). Libraries were sequenced on an Illumina NextSeq 500 with single-end or paired-end 75 bp reads.
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bioRxiv - Cancer Biology 2020Quote: ... Libraries were prepared from 1 μg of total RNA using a TruSeq Stranded Total RNA Kit with Ribo-Zero Human/Mouse/Rat (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... then performed paired-end sequencing of these libraries (38 cycles read 1 + 37 cycles read 2) on a NextSeq 500 (Illumina). After sequencing ...
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bioRxiv - Immunology 2020Quote: ... cDNA libraries were prepared from 1 μg of total RNA using the TruSeq Stranded mRNA kit (Illumina, San Diego, CA) and the Sciclone NGSx Workstation (Perkin Elmer ...
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bioRxiv - Genomics 2021Quote: ... Following immunoprecipitation with Dynabeads Protein G beads (invitrogen) in PCR tubes, samples were subject to Tagmentation (5 µl Tagmentation Buffer, 1 µl Tagmentation DNA Enzyme (Illumina), 19 µl Nuclease free water ...
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bioRxiv - Microbiology 2021Quote: ... Final sequencing pools were loaded at 12pM concentration onto an Illumina MiSeq 300 cycle sequencing run with a 1% PhiX spike-in (Illumina).
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bioRxiv - Plant Biology 2020Quote: ... Libraries were characterized using an Agilent Technologies 2100 Bioanalyzer and quantified before sequencing using NextSeq500 and 1 x 75 b read chemistry (Illumina).
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bioRxiv - Cell Biology 2021Quote: ... 8 bases for index 1 and 8 bases for index 2) using the NextSeq 500 High Output Kit 75-cycles (#FC-404-1005, Illumina) loaded at 1.8pM and including 1 % PhiX ...
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bioRxiv - Systems Biology 2021Quote: ... GBC sequencing libraries were spiked into transcriptome libraries at 1:10 and sequenced on the NextSeq or MiSeq platform (Illumina). Transcriptome libraries were sequenced separately using a NovaSeq5000 S2 300-cycle kit.
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bioRxiv - Systems Biology 2021Quote: ... Samples representative of all treatment groups were multiplexed and sequenced on an Illumina NextSeq500 instrument using high-output 1×75-bp geometry (Illumina).
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bioRxiv - Systems Biology 2020Quote: ... Sequencing was performed using custom read primer oligo1210 (5’-CTTGTGGAAAGGACGAAACACCGGTAATTTCTACTCTTGTAGAT) (HPLC purified, Integrated DNA Technologies) using NextSeq 1 × 75 nt High Output reagents (Illumina).
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bioRxiv - Microbiology 2021Quote: ... The libraries were either paired-end sequenced using 2×75 bp read length (Replica R2 samples) or single-end sequenced using 50 bp read length (Replica 1 samples) on an NextSeq 500 system (Illumina). RNA-seq analysis was performed according to (76) ...
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bioRxiv - Plant Biology 2020Quote: ... and equally pooled in 1 lane of single end 50 bp sequencing in HiSeq 2000 machine (Illumina, San Diego, CA) at the QB3 facility at UC Berkeley.
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bioRxiv - Genomics 2022Quote: Library preparation from 1-10ng rRNA-depleted RNA was performed using NEBNext UltraII Directional RNA Library Preparation Kit (Illumina, #E7760) following the vendor protocols and complementing cleaning steps with NEBNext Sample Purification Beads (#E7767) ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by library construction using non-stranded (Replicate 1) or stranded (Replicates 2 and 3) TruSeq mRNA Library Prep Kit (Illumina). The resulting libraries were sequenced on Hiseq4000.
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bioRxiv - Neuroscience 2022Quote: ... Libraries were sequenced on a HiSeq 2000 and/or HiSeq2500 on a 1×50 bp single read (SR) flow cell (Illumina) at an average of 20M reads/sample ...
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bioRxiv - Cancer Biology 2022Quote: ... Libraries were generated from 1 μg of total RNA using a TruSeq Stranded Total RNA Library Prep Human/Mouse/Rat (48 Samples) (Illumina) per manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... including larger than expected correlations between samples with certain barcode combinations (Supplementary Note 1) and a small but significant number of reads mapping to impossible barcode combinations (combinations of CEL-seq and Illumina barcodes that were not used in the experiment) ...
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bioRxiv - Immunology 2020Quote: ... Use of 250 pg of cDNA with 1/5 reaction of Illumina Nextera XT kit (Illumina, San Diego, CA, USA). The length distribution of the cDNA libraries was monitored using DNA High Sensitivity Reagent Kit on the Perkin Elmer Labchip GX system (PerkinElmer ...
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bioRxiv - Microbiology 2020Quote: ... 4-5 subsamples per replicate were pooled in equimolar concentrations and sequenced on a HiSeq2500 in a 1×50 bp Rapid Run (Illumina). As the first sequencing run did not deliver sufficient data for seven metatranscriptomes ...
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bioRxiv - Developmental Biology 2021Quote: ... Libraries were generated using Kapa Biosystems library preparation kit (#KK8201) and multiplexed libraries were sequenced on a 1×75 flow cell on the HiSeq2000 platform (Illumina). Reads were filtered and trimmed to remove adapter-derived or low-quality bases using Trimmomatic and checked again with FASTQC ...
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bioRxiv - Evolutionary Biology 2021Quote: ... A paired-end Illumina library (DRR029460; see also Supplementary File 1) was constructed using a TruSeq Stranded mRNA LT Sample Prep Kit (Illumina) and an RNA sample extracted from an E ...
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bioRxiv - Microbiology 2021Quote: Pair-end libraries (2×150 bp) were prepared with 1 µg genomic DNA from the evolved strains and sequenced using a MiSeq sequencer (Illumina). The PALOMA pipeline ...
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bioRxiv - Immunology 2022Quote: ... TCRseq libraries were sequenced on an Illumina Novaseq 6000 SP flowcell (67 bp read 1 and 459 bp read 2) (Illumina) to a depth of approximately 20,000 reads.
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bioRxiv - Immunology 2022Quote: ... Targeted RNAseq and Sample Tag libraries were sequenced on an Illumina Novaseq 6000 S4 flowcell (67 bp read 1 and 140 bp read 2) (Illumina) to a depth of approximately 40,000 reads and 10,000 reads per cell ...
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bioRxiv - Immunology 2022Quote: ... whose lengths were about 600 base pairs were sequenced using an Illumina Novaseq 6000 S4 flowcell (67 bp read 1 and 140 bp read 2) (Illumina). Only read2 contained the sequence regarding the definition of T cell clones.