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Citations for Illumina :
301 - 345 of 345 citations for Dnase Free and Rnase Free Distilled Water since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... Pelleted nuclei were resuspended in 25 μL Tn5 transposition mix (12.5 μL 2X Tagment DNA buffer, 1.25 μL Tn5 transposase, and 11.25 μL sterile water; Illumina) and stored in a shaking incubator at 37°C and 500 RPM for one hour ...
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bioRxiv - Neuroscience 2023Quote: ... The pellet was resuspended in 200uL of Transposition Mix (1x TD buffer containing 20 U/mL Superase-In RNase Inhibitor, 40 U/mL RNasin ribonuclease inhibitor and 1.25 µl of Illumina Tagment DNA TDE1 Enzyme per every 100 000 nuclei ...
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bioRxiv - Cell Biology 2022Quote: ... Single cells were dispatched into a 96-plate well containing 5μL 1x lysis buffer containing Murine RNase inhibitor (NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina).
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bioRxiv - Microbiology 2021Quote: ... Ribosomal RNA was removed from the DNase-treated RNA via the Ribo-Zero rRNA Removal Kits (Illumina). RNAseq libraries were constructed using the TruSeq RNA sample prep kit (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... after DNase-treated total RNA was depleted of rRNA using the Ribo-Zero rRNA Depletion kit (Illumina), 1 μg (final volume 100 μL ...
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bioRxiv - Microbiology 2020Quote: ... water (n=9) and sediment (n=9) samples were subjected to bacterial 16S rRNA amplicon profiling by Illumina sequencing ...
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bioRxiv - Immunology 2021Quote: ... Cells were then lysed and fragmented in a single reaction (12.5 µl 2X Illumina TDE buffer, 2.5 µl 1% Tween-20, 2.5 µl 0.2% Digitonin, 5 µl water, 2.5 µl Illumina TDE1 enzyme). Samples were incubated at 37°C for 60 minutes and purified using the Zymo DNA Clean and Concentrator-5 Kit (Zymo) ...
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bioRxiv - Immunology 2020Quote: ... the pellet was resuspended in 50 μL ATAC reaction mix (25uL 2X TD buffer, 2.5 μL Nextera Enzyme, 22.5 μL water, Illumina). The transposase reaction was carried out at 37°C for 30 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... 100 ng of total BH RNA with and without RNase R treatment was then used to construct cDNA libraries by Illumina Stranded Total RNA Prep Ligation with Ribo-Zero Plus (Illumina 20072063) ...
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bioRxiv - Developmental Biology 2024Quote: ... The cell pellet was resuspended in transposition reaction mix (12.5 μl TD-Buffer, 1.25 μl Tn5, 11.25 μl water) (Nextera DNA Library Prep Kit, Illumina, Cat# 15028212) and incubated for 30 min at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... One microgram of total DNAse-treated RNA was then prepared with the TruSeq Stranded mRNA Library Prep Kit (Illumina). Poly-A RNA was purified and fragmented before strand specific cDNA synthesis ...
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bioRxiv - Plant Biology 2023Quote: ... The 5μg total RNA (DNase-treated) was outsourced for the RNA-Seq on Hi-Seq 2000 genome analyzer (Illumina) sequencing platform ...
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bioRxiv - Microbiology 2024Quote: ... One microgram of total DNAse treated RNA was then prepared with the TruSeq Stranded mRNA Library Prep Kit (Illumina). Poly-A RNA was purified and fragmented before strand-specific cDNA synthesis ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA libraries were created from 1 µg DNase-treated RNA with the TruSeq Stranded Total RNA Globin kit (Illumina) and sequenced on the Illumina NovaSeq6000 ...
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bioRxiv - Genetics 2024Quote: ... 100 ng of DNase treated total RNA (RIN > 8) was processed with the TruSeq Stranded mRNA Prep Kit (Illumina) including poly(A ...
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bioRxiv - Cell Biology 2021Quote: ... Libraries that passed quality control were diluted to 2nM DNA content using sterile water and then sequenced on the NextSeq500 (Illumina) at a final concentration of 12pM ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the bands between ∼210 bp and ∼280 bp (tRNAs plus adapters and barcodes) cut out and co-extracted overnight (by soaking in deionized, ultrapure water (Illumina)) ...
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bioRxiv - Plant Biology 2024Quote: ... The RT-PCR products recovered from the gel were dissolved in Milli-Q water and amplified by index PCR using IDT for Illumina DNA/RNA UD index primers (20027213, Illumina) and KOD Plus Neo DNA polymerase ...
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bioRxiv - Cell Biology 2024Quote: ... Libraries that passed quality control were diluted to 2nM DNA content using sterile water and then sequenced on the NextSeq500 (Illumina) at a final concentration of 12pM ...
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bioRxiv - Microbiology 2023Quote: ... Resultant genomic DNA was treated with RNase A and prepared for sequencing using the Nextera XT kit (Illumina Corp., San Diego, CA). Libraries were uniquely dual-indexed and pooled ...
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bioRxiv - Molecular Biology 2021Quote: ... 10μL 2xTagment DNA buffer and 1μL of 100-fold in molecular-grade water diluted DNA tagmentation enzyme (Illumina 20034197, lot 20464427) were added to each 1μL input ...
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bioRxiv - Microbiology 2022Quote: ... a selection of 288 Enterobacterales recovered from water (n=155) and wastewater (n=133) samples underwent paired-end short read sequencing using Illumina (Illumina, USA) NovaSeq 6000 or MiSeq platforms ...
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bioRxiv - Molecular Biology 2023Quote: ... and the nuclei resuspended in 47.5μl of Tagmentation buffer (containing 25μl of 2x Tagmentation buffer from Illumina and 22.5μl of water) before adding 2.5μl of Tn5 enzyme (Illumina Nextera kit ...
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bioRxiv - Immunology 2020Quote: ... RNA was extracted using a modified phenol-chloroform protocol (Chomczynski and Sacchi, 2006) and treated with Baseline-ZERO DNase to remove genomic DNA (Illumina). DNase-treated RNA was then purified using the RNA Clean and Concentrator Kit (Zymo Research ...
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bioRxiv - Genetics 2020Quote: ChIP-seq and DNase-seq libraries were prepared from 1-5 ng ChIP DNA or DNase DNA samples using NEBNext Ultra II DNA library Prep Kit (Illumina). Libraries were sequenced on an Illumina NextSeq 500 with single-end or paired-end 75 bp reads.
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bioRxiv - Cancer Biology 2021Quote: ... by total RNA extraction from confluent cells with Ribo-Zero rRNA depletion and DNAse treatment before cDNA was prepared with the TruSeq RNA and DNA Sample PrepKit (Illumina). 50bp paired-end cDNA libraries sequenced on an Illumina HiSeq 2000 (Genomics Core Facility ...
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bioRxiv - Microbiology 2020Quote: ... 4μg of total DNAse treated RNA was run through the TruSeq Stranded Total RNA LT Sample Prep Kit from Illumina as previously described(3) ...
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bioRxiv - Plant Biology 2022Quote: ... polymorpha wild-type and Mpgun1 mutant spores were prepared using 200 ng of DNase-treated total RNA as a template for the TruSeq Stranded Total RNA kit with Ribo-Zero Plant (Illumina). The libraries were sequenced on an Illumina HiSeq 4000 platform (150 nt paired-end reads ...
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bioRxiv - Microbiology 2021Quote: ... 5 µg of DNase-treated total RNA was subjected to ribosomal RNA removal (RiboZeroTM rRNA Removal for Gram-positive bacteria, Illumina). rRNA depleted samples were then subjected to RNA fragmentation using the Ambion RNA fragmentation kit (AM8740) ...
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bioRxiv - Microbiology 2023Quote: ... Samples were DNAse-treated and sent for library preparation and mRNA-seq by NUcore (Northwestern) on a HiSeq 4000 (Illumina) with 50 bp single-end reads ...
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bioRxiv - Developmental Biology 2022Quote: ... RNA-seq libraries were prepared from a minimum of 200 ng of DNase-treated total RNAs with the TRuSeq Stranded mRNA protocol (Illumina). Between 59M and 130M reads were sequenced per sample in a 100bp paired-end format using a NovaSeq 6000 (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... DNase-treated RNA extracts were used for library preparation using the TruSeq Stranded mRNA sample preparation kit (Illumina, San Diego, California) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... 5 μg of DNase-treated total RNA was first subjected to ribosomal RNA removal (RiboZero™ rRNA Removal for Gram-positive bacteria, Illumina). rRNA depleted samples were then subjected to RNA fragmentation using the Ambion RNA fragmentation kit (AM8740) ...
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bioRxiv - Cell Biology 2023Quote: ... DNAse-treated RNA extracts were processed for library preparation using the Truseq Stranded mRNA sample preparation kit (Illumina, San Diego, California) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: DNAse-treated RNA extracts were processed for library preparation using the Truseq Stranded mRNA sample preparation kit (Illumina, San Diego, California) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: Sequencing libraries were prepared by the University of Chicago Genomics Facility from DNase I treated RNA using Illumina RiboZero (Illumina #MRZY1306) and Illumina TruSeq library prep kits ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNA-seq libraries were prepared starting from 200 ng of DNase-treated total RNA according to the Illumina TruSeq Stranded Total RNA protocol (Document # 1000000040499 v00, © 2021 Illumina, Inc.). The libraries qualities were assessed by running a 2100 Bioanalyzer DNA 1000 chip (Agilent Technologies ...
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bioRxiv - Genomics 2024Quote: ... Total RNA-seq libraries were generated from 1 μg of DNase-treated RNA using the TruSeq Stranded total RNA library preparation kit (Illumina, Cat# 15031048) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... miRNA libraries were prepared from the isolated EV RNA and a water control using the TruSeq Small RNA Library Preparation Kit (RS-200-0012, Illumina, San Diego, CA, USA) using the manufacturer instructions with the modification of performing the adaptor ligation in the presence of 15% polyethylene glycol in order to reduce bias by driving the ligation reactions towards completion as determined by the Extracellular RNA Communication Consortium (46) ...
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bioRxiv - Plant Biology 2020Quote: ... One μg of DNase-treated total RNA was used for library construction using TruSeq Stranded mRNA Library Prep Kit (Illumina Inc, CA, USA) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... 250ng of DNase I-treated total RNA was depleted of cytoplasmic and mitochondrial ribosomal RNA (rRNA) using Ribo-Zero Gold (Illumina, San Diego, CA). After purification ...
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bioRxiv - Genomics 2020Quote: Messenger RNA capture based libraries were prepared starting from 8.5 µL DNase treated and spike-in supplemented RNA eluate using the TruSeq RNA Exome Library Prep Kit (Illumina, San Diego, CA, USA). Each sample underwent individual enrichment according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... The 16S rRNA gene V4-V5 region was amplified from each water sample and sequenced using the Illumina MiSeq platform (Illumina Inc., San Diego, CA, USA) (Tremblay et al. ...
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bioRxiv - Neuroscience 2022Quote: The RNA samples were DNase-treated and then underwent poly-A enrichment and library construction using a TruSeq v2 Library Preparation Kit (Illumina, San Diego, CA, USA), followed by 150 bp paired-end sequencing on an Illumina HiSeq 3000 platform at the Max Planck-Genome center (Cologne ...
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bioRxiv - Genomics 2020Quote: Small RNA libraries were prepared starting from 5 µL DNase treated and spike-in supplemented RNA eluate using a TruSeq Small RNA Library Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer’s protocol with two minor modifications(1) ...