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Citations for Illumina :
251 - 295 of 295 citations for Ubiquitin Conjugating Enzyme E2 K UBE2K Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... 25 μL TD buffer and 2.5 μL TDE1 enzyme from Nextera DNA Library Prep Kit (Illumina, cat. no. FC-121-1030)) ...
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bioRxiv - Immunology 2021Quote: ... 1.5 ng cDNA was tagmented using 0.5 μl TruePrep Tagment Enzyme V50 and 1x TruePrep Tagment Buffer L (TruePrep DNA Library Prep Kit V2 for Illumina, Vazyme), followed by an incubation step at 55 °C for 10 min ...
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bioRxiv - Immunology 2022Quote: ... The remaining lysis supernatant containing the nuclei were immediately placed into a transposase reaction using the Tagment DNA TDE1 enzyme kit (Illumina 20034197) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: The OmniATAC transposition reaction was performed with 50000 mES cells as previously described (Corces et al., 2017) using the Tagment DNA TDE1 Enzyme (Illumina, #20034197). DNA was purified using the PCR clean-up MinElute kit (Qiagen ...
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bioRxiv - Cancer Biology 2020Quote: ... Extracted nuclei was processed for TN-5 mediated tagmentation using the Illumina Tagment DNA Enzyme and buffer kit (Nextera Illumina # 20034210) ...
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bioRxiv - Genomics 2021Quote: ... and 1µM Dexamethasone or 0.1% ethanol for 3 hours. Transposition was performed using the OmniATAC protocol (Corces et al. 2017) and the tagment DNA TDE1 enzyme (Illumina, 20034197). DNA was purified using the MinElute PCR purification kit (Qiagen) ...
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bioRxiv - Cancer Biology 2022Quote: ... The cell pellet was then mixed and incubated at 37°C for 1h min with the transposition reaction mix prepared using Illumina Tagment DNA Enzyme and Buffer kits (Illumina, 20034197). DNA was then purified using DNA Clean and Concentrator kit (Zymo ...
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bioRxiv - Developmental Biology 2022Quote: ... After centrifugation supernatant was used for RNA cytosolic fraction by adding to 1 ml of TRIZOL and isolated nuclei of CMs were incubated in 30 µl and of EC in 10 µl transposase mixture with 5% Nextera Tagment DNA Enzyme TDE (Illumina #15027916) in transposition buffer (20 mM Tris-HCl pH 7.6 ...
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bioRxiv - Cancer Biology 2024Quote: ... Isolated nuclei were resuspended in 50 μL of Transposition mix (Illumina Tagment DNA Enzyme 100 nM, TD-Buffer 2X (kit from Illumina, 20034197), PBS 0.33% ...
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bioRxiv - Neuroscience 2024Quote: ... Illumina sequencing libraries were generated from the amplified cDNA samples using the Illumina Tagment DNA Enzyme and Buffer kit (Illumina, CA). These libraries were examined by Qubit and Bioanalyzer ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 x 105 mESCs and 5 x 104 S2 cells were resuspended in Tagment DNA Buffer and treated with 3 µL TDE1 Tagment DNA Enzyme (Illumina 20034197). After thorough mixing ...
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bioRxiv - Genomics 2023Quote: ... Nuclei isolation and transposition followed the OMNI ATAC-seq protocol as previously reported11 using the Illumina Tagment DNA TDE1 Enzyme & Buffer Kit (Illumina #20034197). Libraries were generated using Illumina Nextera CD Indexes (Illumina #20018708 ...
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bioRxiv - Genomics 2024Quote: ... Sequencing adapters were added to the bead-bound DNA by Tagmentation with the Illumina Tagment DNA Enzyme and Buffer Kit (Illumina, 20034197). After washes ...
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bioRxiv - Evolutionary Biology 2021Quote: ... we combined 3 μl of each sample (approx 2.24 ng) with a small quantity of a Nextera™ tagment DNA enzyme (Illumina catalogue #15027865). To decrease costs ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We then prepared tagmentation-based whole genome libraries for low coverage sequencing by enzymatically shearing DNA diluted to approximately 2.5 ng/ul using the Illumina Tagment DNA TDE1 Enzyme and Buffer Kits (Illumina, San Diego, CA) at 55°C for 5 minutes ...
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA was diluted to approximately 2.5 ng/μL and enzymatically sheared using the Illumina Tagment DNA TDE1 Enzyme and Buffer Kits (Illumina, San Diego, CA) at 55°C for 5 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... The resulting cell nuclei were subjected to incubation for 30 min with Tagment DNA TDE1 Enzyme and Buffer (Illumina, #FC-121-1030). This step fragmented the chromatin and inserted sequencing adapters ...
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bioRxiv - Genomics 2023Quote: ... Two biological replicates of 7.5 × 104 cells for each condition were then isolated for direct processing with Nextera Tn5 enzyme (Illumina, FC-131-1096). Samples were treated as previously described (77 ...
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bioRxiv - Developmental Biology 2021Quote: ... The nuclei were suspended in a transposition mix (TD Tagment DNA Buffer, Illumina Catalog No. 15027866, and TDE1 Tagment DNA Enzyme, Illumina Catalog No. 15027865), and incubated at 37°C to generate DNA fragments ...
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bioRxiv - Molecular Biology 2021Quote: ... Beads were resuspended in 25 μl of 2X TD Buffer and combined with Tn5 enzyme from the Nextera DNA Sample Preparation Kit (Illumina, FC-121-1030) and water to final volume 50 μl ...
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bioRxiv - Molecular Biology 2021Quote: ... Granulosa cells were lysed and tagmentation was performed on the nuclei using the Illumina Tagment DNA TDE1 Enzyme and Buffer Kits (Illumina, San Diego, USA). DNA purification was performed using the Monarch PCR & DNA Cleanup Kit (NEB ...
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bioRxiv - Genomics 2020Quote: ... The dissolved DNA was then tagmented using 0.6 μl TD Tagmentation buffer and 0.3 μl ATM Tagmentation enzyme from Nextera DNA Library Prep Kit (Illumina, catalog no. FC-121-1030) for 5min at 55 °C ...
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bioRxiv - Genomics 2020Quote: ... The resulting nuclei were resuspended in 50µl of transposition reaction buffer (25µl of 2x TD buffer from the Nextera kit, Illumina; 2.5µl of Tn5 transposase enzyme from the Nextera kit, Illumina; 22.5µl of nuclease free water), and incubated at 37°C for 90 min for chromatin tagmentation ...
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bioRxiv - Immunology 2021Quote: ... Chromatin fragments bound to magnetic beads were resuspended in 30 μl Tagmentation mix (14 μl H2O, 15 μl Tagment DNA Buffer [Illumina, order no. 15027866], 1 μl Tagment DNA Enzyme [Illumina, order no. 15027865]) and incubated for 10 minutes at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... The cell pellet was resuspended in Illumina TD buffer and 2.5 µl of Tn5 enzyme was added to the transposition reaction (Illumina, Cat # FC-121-1030). Nuclei were incubated at 37°C for 60 minutes and fragmented DNA was purified using the MinElute kit (Qiagen ...
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bioRxiv - Genetics 2024Quote: ... nuclei were counted by trypan blue staining and 50,000 nuclei were then recruited for Tn5 transposase reaction by using Illumina Tagment DNA Enzyme and Buffer kit (Illumina, California, San Diego, USA). For head kidney samples ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We prepared libraries for low-coverage whole genome sequencing using a tagmentation based protocol and the Illumina Tagment DNA TDE1 Enzyme and Buffer Kit (Illumina, San Diego, CA). Briefly ...
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bioRxiv - Immunology 2024Quote: ... 60,000 naive CD4+ T cells from each biological replicate were spun down for nuclei isolation followed by transposition using Tn5 transposase from the Tagment DNA (TD) TDE1 Enzyme and Buffer Kit (Illumina, cat. no. 20034197). After transposition ...
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bioRxiv - Genomics 2020Quote: ... The remaining 1μl re-dissolved contents were tagmented using 0.6 μl TD Tagmentation buffer and 0.3 μl ATM Tagmentation enzyme from Nextera XT DNA Library Prep Kit (Illumina, catalog no. FC-121-1030) for 5min at 55 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... Transposition was performed directly on nuclei using 25 µl tagmentation reaction mix (Tagment DNA Buffer #15027866, Tagment DNA Enzyme #15027865 from Illumina Tagment DNA kit #20034210). Tagged DNA was subjected to PCR amplification and library indexing ...
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bioRxiv - Genetics 2022Quote: 1184 recombinant inbred lines of the S6 generation of the Global MAGIC population were genotyped from DNA extracted from leaf tissue using Genotyping-by-Sequencing (GBS, with the enzyme ApeKI) across 14 lanes of 96-sample multiplexed sequencing (100 bp single-end Illumina, Phred+33 fastq format) as reported in Zaw et al ...
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bioRxiv - Microbiology 2020Quote: ... One nanogram of quantified DNA was sheared enzymatically at 55°C for 5 min using the Illumina Nextera XT tagmentation enzyme (Illumina, San Diego, CA, USA). Tagmented DNA fragments were enriched by 10 cycles of PCR amplification using PCR master mix and primers with the index from Illumina ...
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bioRxiv - Immunology 2024Quote: ... transposition reaction was done with 350 rpm mixing and using the transposase from the Illumina Tagment DNA TDE1 Enzyme and Buffer Kit (Illumina, San Diego, CA, USA). Libraries were quantified using KAPA Library Quantification kit (Roche ...
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bioRxiv - Plant Biology 2023Quote: ... RADseq library was prepared with HindIII and NlaIII restriction enzymes for DNA digestion and sequenced using Illumina NovaSeq 6000 (Illumina, San Diego, CA, USA). Raw sequence data is available on NCBI SRA under the project number PRJNA997794 ...
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bioRxiv - Immunology 2024Quote: ... Transposition was performed directly on nuclei using 25µl tagmentation reaction mix (Tagment DNA Buffer #15027866, Tagment DNA Enzyme #15027865 from Illumina and Digitonin #G9441 from Promega). After pelleting the nuclei by centrifuging at 500g for 10 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Transposition was performed directly on nuclei using 25 μl tagmentation reaction mix (Tagment DNA Buffer #15027866, Tagment DNA Enzyme #15027865 from Illumina Tagment DNA kit #20034210). Tagged DNA was subjected to PCR amplification and library indexing ...
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bioRxiv - Plant Biology 2021Quote: ... for 96-plex Genotyping-by-Sequencing (GBS) library preparation using the ApeKI restriction enzyme and sequenced (1 × 100 bp) on the Illumina HiSeq 2500 (Illumina, Inc., San Diego, CA, USA) platform.
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bioRxiv - Genomics 2020Quote: A total of 720 metagenomic libraries were prepared using Illumina-specific Nextera DNA transposase adapters and a Tagment DNA Enzyme and Buffer Kit (Illumina, San Diego, CA, cat. no. 20034197) (modified from Baym et al ...
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bioRxiv - Neuroscience 2020Quote: ... the nuclei-containing pellet was resuspended with 50 µl of the transposase reaction mix which contained 25 µl of the 2xTD reaction buffer and 2.5 µl of Tn5 Transposase (Nextera DNA Library Preparation Kit, Illumina, FC-121-1030; note: these reagents are now available in the Illumina Tagment DNA TDE1 Enzyme and Buffer Kit). Transposition was performed for 30 minutes at 37°C after which the transposed DNA was purified using MinElute PCR Purification Kit (Qiagen ...
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bioRxiv - Cancer Biology 2023Quote: ... Triplicates were pooled for a target total cell number of 60,000 in 50uL to load less than 20uL into the 10X controller or perform TDE1-based tagmentation reactions on small volumes (Illumina Tagment DNA TDE1 Enzyme and Buffer Kit). The 10X Genomics Chromium Platform was used to generate a targeted 5,000 single cell Gel Bead-In-Emulsions (GEMs ...
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bioRxiv - Genomics 2022Quote: ... the ScriptSeq™ Index PCR Primers (Sets 1 to 4) and the FailSafe™ PCR enzyme system (all sourced from Epicentre®/Illumina® Inc., Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Single-cell antibody repertoire libraries were sequenced on an Illumina NovaSeq 6000 system (Illumina RTA Version: V3.4.4) using a 26 x 91 bp read configuration.
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bioRxiv - Cancer Biology 2020Quote: ... Antibody tag libraries were sequenced with an Illumina NextSeq 550 75-cycle High Output Kit (Illumina, cat. no. 20024906) in paired-end mode ...
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bioRxiv - Bioengineering 2022Quote: ... libraries were pooled at a ratio of 80% cellular RNA to 20% oligonucleotide barcoded antibodies and sequenced with NextSeq 1000/2000 kit (Illumina) using the following read length ...
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bioRxiv - Microbiology 2023Quote: ... RNAs extracted from immunoprecipitated complexes using Ago2 and GFP antibody were used to generate small RNA sequencing libraries using TruSeq Small RNA Kit (Illumina), according to the manufacturer’s instructions ...