Labshake search
Citations for Illumina :
201 - 250 of 277 citations for Transcription Factor SOX 10 SOX10 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: ... The transposed DNA was amplified by PCR for 10-12 cycles by using the Nextera DNA Library Preparation Kit and Nextera XT Indexing Kit (Illumina). The library DNA within the 150-to 500-bp range was enriched by AMPure XP beads (Beckman Coulter) ...
-
bioRxiv - Immunology 2020Quote: ... The samples were mixed in equimolar ratios to obtain 30 μl of a final concentration of 10-nM and sequenced by Illumina MiSeq (v3 kit ...
-
bioRxiv - Genomics 2019Quote: ... single-end mode) at 12 pM loading concentration with 10% PhiX spike-in (PhiX Control V3 [Illumina FC-110-3001]) following the manufacturer’s instructions.
-
bioRxiv - Cancer Biology 2022Quote: ... Gene expression libraries and barcode enrichment libraries were pooled in an approximately 10:1 molar ratio and libraries were sequenced on the NovaSeq platform (Illumina).
-
bioRxiv - Molecular Biology 2022Quote: ... libraries were submitted to the Genomics and Microarray Shared Resource at the University of Colorado Anschutz Medical Campus for sequencing of 10 million paired end reads per sample on the NovaSEQ6000 (Illumina).
-
bioRxiv - Genomics 2022Quote: ... Beeline was used to convert raw image (.idat) files to genotype call (.gtc) files.10 The data were analyzed using GenomeStudio® 2.0 software (Illumina). 11
-
bioRxiv - Microbiology 2022Quote: ... The 10-pM final library was sequenced on the Illumina MiSeq sequencing platform (2 × 150bp paired-end reads) (Illumina Inc.).
-
Neural circuit-wide analysis of gene expression during deafening-induced destabilization of birdsongbioRxiv - Neuroscience 2022Quote: ... of amplified cDNA was added to 10 μL Tagmentation Buffer (Buffer TD from the Nextera XT DNA Sample Prep Kit, Illumina), 1 μL nuclease-free water ...
-
bioRxiv - Cancer Biology 2023Quote: ... Paired-end sequencing was performed for all libraries (read 1:28 bp, index read: 10 bp, read2: 82 bp) on a NovaSeq 6000 (Illumina) using NovaSeq SP Reagent Kits (100 cycles).
-
bioRxiv - Microbiology 2023Quote: ... was performed at 10 million reads/sample in single-end mode with 75 nt read length on the NextSeq 500 platform (Illumina) using High output sequencing kits ...
-
bioRxiv - Molecular Biology 2023Quote: Multiplexed library pools were generated at 10 nM concentrations of each library and sequenced on NovaSeq 6000 system (Illumina, v1.5). Sequencing was paired end with Read1N and Read2N sequenced with 50 cycles each ...
-
bioRxiv - Molecular Biology 2023Quote: ... paired-end cDNA libraries were prepared from 10 μg of total RNA by polyA selection using the TruSeq Stranded mRNA kit (Illumina) according to manufacturer’s instructions ...
-
bioRxiv - Genomics 2023Quote: ... The PhiX Control library was spiked in the sequencing sample at 10% (v/v) (Illumina, cat. no. FC-110-3001).
-
bioRxiv - Genomics 2022Quote: ... We centrifuged at 500 x g for 10 minutes at 4°C and resuspended cells in the transposition reaction mix (25 µL 2X TD buffer (Illumina), 2.5 µL TDE1 Tn5 transposase (Illumina) ...
-
bioRxiv - Immunology 2023Quote: ... Transcriptome profiles of individual cells were determined by 10× Genomics-based droplet sequencing and libraries were sequenced with paired-end reads on an Illumina NovaSeq 6000 (Illumina).
-
bioRxiv - Microbiology 2023Quote: Sequencing libraries were prepared from the same DNA extracted from the individual collected in October 2010 and previously reported in [10] using the Nextera kit (Illumina) according to the manufacturer’s instructions and concentrations measured using the QuantIT kit (Molecular Probes ...
-
bioRxiv - Genomics 2023Quote: ... ChIP DNA was then prepared for Illumina multiplex-sequencing with 10 samples per lane at 65 bp single end and sequenced on Illumina HiSeq 2500 (Illumina).
-
bioRxiv - Genomics 2023Quote: ... along with 10 ul of Nextera DNA Flex Dual Index adapters (Illumina: IDT for Illumina DNA/RNA UD indexes: 20027213) as primers ...
-
bioRxiv - Microbiology 2023Quote: ... and RNA sequencing to a depth of 10 million 75 bp or 100 bp single end reads using a Nextseq500 or NextSeq2000 sequencing system (Illumina). Data were analysed using CLC Genomics Workbench version 21.0.5 (Qiagen) ...
-
bioRxiv - Molecular Biology 2024Quote: ... and clustered on an Illumina Novaseq 6000 S4 flow cell followed by 150bp paired-end sequencing with inclusion of 10-15% spike-in PhiX Control v3 library (Illumina). Controlled access to DNase-seq ...
-
bioRxiv - Microbiology 2024Quote: ... All samples were pooled to a final concentration of 5-10 ng/µL and sequenced on a NextSeq 500 (Illumina). The demultiplexed data was assembled and SNPs detected with Pilon software using reference genome sequences.84
-
bioRxiv - Neuroscience 2024Quote: ... sequencing adaptors P5 and P7 were introduced following the 10xGenomics dual-index setup: 20-50ng PCR product was added to library PCR mix (50% KAPA, 10% Betaine, 20% primer Dual index (Illumina)) ...
-
bioRxiv - Neuroscience 2024Quote: ... sequencing adaptors P5 and P7 were introduced following the 10xGenomics dual-index setup: 20-50ng PCR product was added to library PCR mix (50% KAPA, 10% Betaine, 20% primer Dual index (Illumina)) total 20µl ...
-
bioRxiv - Neuroscience 2024Quote: ... sequencing adaptors P5 and P7 were introduced following the 10xGenomics dual-index setup: Eluted PCR product was added to library PCR mix (50% KAPA, 10% Betaine, 20% primer Dual index (Illumina)) ...
-
bioRxiv - Molecular Biology 2024Quote: ... The libraries were adjusted to 10 pM spiked in with 15% PhiX Control v3 Library and sequenced on the NextSeq550 and HiSeq2500 (Illumina) platform (R1 – 54 cycles ...
-
bioRxiv - Immunology 2021Quote: Single-cell antibody repertoire libraries were sequenced on an Illumina NovaSeq 6000 system (Illumina RTA Version: V3.4.4) using a 26 x 91 bp read configuration.
-
bioRxiv - Cell Biology 2020Quote: ... Libraries were then indexed and PCR amplified (10 cycles) and sequenced on Illumina HiSeq 2500 sequencing platform following the manufacture’s protocols (Illumina, Hayward CA.).Sequence reads were aligned to GRCh37-lite using Burrows-Wheeler Aligner (BWA ...
-
bioRxiv - Cell Biology 2019Quote: ... 10 samples were sequenced with 151 bp paired-end reads on a single lane of NextSeq 500 (Illumina, San Diego, CA) at the Stanford Functional Genomics Facility.
-
bioRxiv - Neuroscience 2019Quote: ... allowing for 10 ‘barcodes’ per sample and sequenced on Illumina HiSeq 2500 or NextSeq 500 platforms (Illumina, San Diego, CA, USA).
-
bioRxiv - Neuroscience 2020Quote: ... The cluster was generated by cBot with the library diluted to 10 pM and then was sequenced on the Illumina NovaSeq 6000 (Illumina, USA). The library construction and sequencing were performed by Shanghai Sinotech Genomics Corporation (China).
-
bioRxiv - Neuroscience 2022Quote: ... Nuclei were pelleted (500xg, 10 min, 4 °C) and tagmented with the Nextera DNA Library Prep Kit (Illumina, FC-121-1030) for 1 h at 37 °C ...
-
bioRxiv - Genomics 2022Quote: ... pH 7.4, 5 mM MgCl2, 10% dimethyl formamide, 0.33x PBS, 0.01% digitonin, 0.1% Tween-20, 100 nM Illumina Tn5 Transposase (Illumina, 20034197)) ...
-
bioRxiv - Immunology 2020Quote: ... Supernatant was discarded and nuclei were re-suspended in 50 μl reaction buffer containing 5.0 μl Tn5 transposase and 10 μl of 5 × TTBL buffer (TruePrepTM DNA Library Prep Kit V2 for Illumina, Vazyme Biotech). The reaction was incubated at 37°C for 30 min ...
-
Induction of Dopaminergic Neurons for Neuronal Subtype-Specific Modeling of Psychiatric Disease RiskbioRxiv - Neuroscience 2021Quote: ... RNA fragmentation was performed at 94°C for 6 minutes and 10 PCR cycles were used during library amplification with TruSeq single-index adapters (Illumina, #20020492). Final library concentrations were quantified with both Qubit fluorometric quantification (DNA dsDNA HS kit ...
-
bioRxiv - Cancer Biology 2022Quote: ... and sequenced on a total of 10 Hiseq 2500 lanes using the 125bp paired-end sequencing method (Illumina, San Diego, CA). Both reads of each sample were trimmed for adapters and low-quality bases using Trimmomatic software and aligned with reference human hg19 genome and ensemble v70 transcripts using Tophat software as stranded libraries ...
-
bioRxiv - Developmental Biology 2022Quote: ... After centrifugation supernatant was used for RNA cytosolic fraction by adding to 1 ml of TRIZOL and isolated nuclei of CMs were incubated in 30 µl and of EC in 10 µl transposase mixture with 5% Nextera Tagment DNA Enzyme TDE (Illumina #15027916) in transposition buffer (20 mM Tris-HCl pH 7.6 ...
-
bioRxiv - Synthetic Biology 2022Quote: ... Sequencing library preparation was then performed using Stranded Total RNA Prep Ligation kit with Ribo-Zero Plus for rRNA depletion and by using 10 bp IDT for Illumina indices (all from Illumina, USA). Sequencing was done on a NextSeq2000 instrument in 2×50 bp paired-end mode ...
-
bioRxiv - Microbiology 2023Quote: ... Samples were equimolarly pooled with 10% PhiX control DNA and sequenced on an Illumina MiSeq using a MiSeq 2 × 250 v2 kit (Illumina, Inc.). Due to the number of samples ...
-
bioRxiv - Developmental Biology 2023Quote: ... 4-10 ng of the full-length cDNA was used as input for preparing Nextera XT (Illumina, cat. # FC-131-1024) libraries following the manufacturer’s recommendations ...
-
bioRxiv - Biophysics 2023Quote: ... 100-120 μl pooled-denatured 20 pM library and 10-20 μl 20 pM PhiX control library (Illumina, FC-110-3001) to provide a reasonable spot density in general ...
-
bioRxiv - Molecular Biology 2024Quote: ... Libraries were then indexed and PCR amplified (10 cycles) and sequenced on Illumina HiSeq 2500 sequencing platform following the manufacture’s protocols (Illumina, Hayward CA).
-
bioRxiv - Microbiology 2023Quote: ... Library preparation was performed using the Illumina DNA Prep Kit and IDT 10 bp UDI indices (Illumina, San Diego, CA, USA), hybrid whole genome sequencing was carried out on an Illumina NextSeq 2000 (2 × 151 bp reads ...
-
bioRxiv - Genomics 2020Quote: ... Five size-fractions were selected ranging from 470 bp to 10 kb to construct sequencing libraries following the manufacturer’s protocols (Illumina, San Diego, CA). The TruSeq DNA Sample Preparation Kit version 2 with no PCR amplification (PCR-free ...
-
bioRxiv - Neuroscience 2020Quote: ... and 40 from each of the 10 donors) were prepared with the TruSeq Stranded mRNA kit from Illumina (San Diego, CA, USA) and sequenced on a HiSeq 4000 sequencer (Illumina ...
-
bioRxiv - Developmental Biology 2022Quote: ... The library samples were then diluted to a 10 nM concentration and sequenced on an Illumina NovaSeq 6000 platform (Illumina, San Diego) for PE150 sequencing by Annoroad Gene Tech ...
-
bioRxiv - Cancer Biology 2020Quote: ... Drop-seq denatured libraries were loaded at 1.3pM final concentration and were spiked in with 10% of 1.8pM PhiX Control v3 (Illumina Cat# FC-110-3001). Sequencing specifications were as follows ...
-
bioRxiv - Developmental Biology 2020Quote: ... RNA was extracted from five biological replicates of each of the 222 and ii2 diet groups (10 samples in total) using the TruSeq Stranded mRNA-seq kit (Illumina, DA, USA). The samples were sequenced in one flowcell on the NextSeq 500 producing paired end reads of 75bp.
-
bioRxiv - Genomics 2020Quote: ... using 5% PhiX without dark cycles (n = 11) or 10-20% PhiX with 7 dark cycles (protocol provided by Illumina, n = 49). A maximum of 12 samples were pooled in one sequencing run resulting in 19.05 million [17.05 – 21.72] single-end reads per sample on average (supplementary table 3).
-
bioRxiv - Cancer Biology 2020Quote: ... Antibody tag libraries were sequenced with an Illumina NextSeq 550 75-cycle High Output Kit (Illumina, cat. no. 20024906) in paired-end mode ...
-
bioRxiv - Microbiology 2021Quote: ... Fragments were then indexed with 10 nucleotide barcode sequences using Nextera XT Index Kit v2 Set D primers (Illumina, San Diego, California, USA). Indexing PCR reactions were run in triplicate with a volume of 25 μL using 12.5 μL NEB Next High-Fidelity Master Mix ...