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Citations for Illumina :
151 - 200 of 291 citations for 3 3 Bromomethyl phenyl thiophene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: Gene analysis was performed thanks to the GenoBird platform by 3’RNA-sequencing profiling using a NovaSeq 6000 (Illumina). The quality of raw sequence reads was assessed by FastQC ...
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bioRxiv - Cell Biology 2024Quote: ... Libraries were prepared using the 10x genomics chromium 3’ gene expression solution v3.1 and sequenced on a NovaSeq6000 (Illumina). Read counts were analysed using Seurat (v5) ...
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bioRxiv - Cell Biology 2024Quote: ... After 2-3 passages in complete DMEM with 10% FBS gDNA was extracted to confirm the genotype by Illumina amplicon sequencing and live cell microscopy was performed.
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bioRxiv - Cancer Biology 2021Quote: ... libraries were prepared using a Chromium Single Cell 3’ Library & Gel Bead kit v2 (10x Genomics, PN-120237) and sequenced using a NextSeq 500 (Illumina). The mean number of reads per cell was approximately 25,000 and the median number of genes detected per cell was approximately 2,000.
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bioRxiv - Cell Biology 2020Quote: ... Indexed sequencing libraries were constructed using the Chromium Single-Cell 3’ library kit (10X Genomics) and sequenced on NovaSeq 6000 (Illumina) with the following parameters ...
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bioRxiv - Genetics 2021Quote: ... 3) SpliceAI (SAI)11 scores were retrieved using a script adapted from the SAI github repository (https://github.com/Illumina/SpliceAI) which allows spliceAI to score custom sequences ...
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bioRxiv - Microbiology 2022Quote: ... Total RNAs samples (3 μg) were depleted from ribosomal RNA using the bacteria Ribo-Zero® rRNA Removal kit (Illumina) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... Multiple mate-paired libraries (3, 8, 12 and 16 kb) were also constructed using the Nextera Mate-Paired Library Construction kit (Illumina). Libraries were sequenced on the Illumina HiSeq 2500 sequencer using the Illumina TruSeq PE Cluster kit v3 and TruSeq SBS kit v3 (101 ...
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bioRxiv - Immunology 2020Quote: ... cDNA was harvested and diluted to 0.1–0.3 ng/μl and libraries were prepared in 96-well plates using a Nextera XT DNA Sample Preparation kit (Illumina) according to the protocol supplied by Fluidigm ...
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bioRxiv - Neuroscience 2020Quote: Tagmentation was performed per pool by adding 3 μL double-stranded cDNA sample to 5.5 μL Nextera TD buffer (Illumina, 15027866), 2.5 μL NF H2O and 1.0 μL TDE1 Enzyme (Illumina ...
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bioRxiv - Systems Biology 2020Quote: ... 8 pools of 24 randomized libraries were sequenced in 3 lanes at 100 bp single-end on the HiSeq 2500 (Illumina) using TruSeq SBS Kit v4 reagents (Illumina).
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bioRxiv - Neuroscience 2020Quote: ... The cDNA was then fragmented and amplified for 3’ prime end sequencing with the Nextera XT DNA sample prep kit (Illumina). The libraries were purified ...
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bioRxiv - Genomics 2020Quote: ... sequencing libraries were prepared with the QuantSeq 3’ mRNA-Seq Library Prep Kit FWD (Lexogen) and sequenced on a HiSeq 4000 Sequencing System (Illumina) using the HiSeq3000/4000 SR Cluster and SBS Kit (Illumina).
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bioRxiv - Microbiology 2021Quote: ... the sgRNA coding sequences integrated in the genomic DNA from the initial and 3-times selected populations were amplified by touch-down PCR and sequenced by Illumina deep sequencing ...
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bioRxiv - Molecular Biology 2021Quote: ... 150 ng of RNA was used for library preparation with the Lexogen QuantSeq 3’ mRNA-Seq Library Prep Kit (FWD) from Illumina. Quality control of the sequencing libraries was performed with both Qubit™ (DNA HS Assay kit ...
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bioRxiv - Cancer Biology 2020Quote: The RNA-seq libraries (n=3 per experimental group) were prepared using the NEBNext Ultra II RNA library prep kit from Illumina (New England Biolabs Inc. ...
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bioRxiv - Cancer Biology 2020Quote: ... and the analysis was run using sequencing reads of 3 or higher from 454 data and 10 or greater reads from Illumina. Single cell sequencing was performed as described previously (Mann et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by library construction using non-stranded (Replicate 1) or stranded (Replicates 2 and 3) TruSeq mRNA Library Prep Kit (Illumina). The resulting libraries were sequenced on Hiseq4000.
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bioRxiv - Neuroscience 2022Quote: ... cells were harvested and prepared with 10X Chromium Single Cell 3’ Reagents V3 and sequenced on a NovaSeq 6000 (Illumina) using a S2 flow cell at 2 × 100bp ...
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bioRxiv - Cell Biology 2022Quote: ... RNA library was constructed with 10x-Genomics Single Cell 3’ RNA sequencing kit v3 as previously described(18) sequenced using a NextSeq500 (Illumina). Cell clusters were characterized using an automated annotation tool(19 ...
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Patient-associated mutations in Drosophila Alk perturb neuronal differentiation and promote survivalbioRxiv - Cancer Biology 2022Quote: ... and libraries prepared using the normal workflow for Single Cell 3’ v3 libraries (10X Genomics) and sequenced on the NextSeq 500 platform (Illumina). Initial preprocessing of scRNA-seq datasets began with demultiplexing ...
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bioRxiv - Cancer Biology 2022Quote: ... using primers DCF01 5’-CTTGTGGAAAGGACGAAACACCG-3’ and DCR03 5’-CCTAGGAACAGCGGTTTAAAAAAGC-3’ and subjected to single-end 75 bp (SE75) high-throughput sequencing using a NextSeq550 (Illumina).
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bioRxiv - Developmental Biology 2021Quote: ... and cDNA synthesis and library construction were performed as per the manufacturer’s protocol for the Chromium Single Cell 3′ v2 protocol (10x Genomics; PN-120233) and sequenced on an Hiseq4000 (Illumina). 10x CellRanger (version 3.0.2 ...
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bioRxiv - Neuroscience 2022Quote: ... scRNA-seq libraries were prepared with the Chromium Single Cell 3′ Library & Gel Bead Kit v.3 (10x Genomics, PN-1000075).Ready 10X libraries were sequenced paired end (R1:28, R2: 89 cycles) on NovaSeq (Illumina).
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bioRxiv - Cancer Biology 2022Quote: ... performed the library preparation for WGS of parental and resistant cells of cell lines #3 and #9 as well as a control tail samples corresponding to line #3 with the TruSeq DNA PCR-free Kit (Illumina) and the 150 bp paired-end sequencing on a HiSeq X (Illumina).
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bioRxiv - Cancer Biology 2020Quote: ... 500 ng RNA was prepared with a commercially available kit according to the manufacturer’s instruction (QuantSeq 3′ mRNA-Seq Library Prep Kit FWD for Illumina and PCR Add-on Kit for Illumina, Lexogen). Sequencing was performed on an Illumina HiSeq2500 in 50bp single-read mode.
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bioRxiv - Cancer Biology 2020Quote: ... 500 ng alkylated RNA was used and prepared with a commercially available kit (QuantSeq 3′ mRNA-Seq Library Prep Kit FWD for Illumina and PCR Add-on Kit for Illumina ...
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bioRxiv - Developmental Biology 2020Quote: ... and two different mate-pair libraries (3 kb and 5 kb) were prepared with a Nextera Mate Pair Library Prep Kit (Illumina). Sequencing libraries were run on the Illumina Hiseq 2500 sequencer with a read length of 150 bp ...
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bioRxiv - Developmental Biology 2020Quote: ... Libraries were constructed with version 2 (stage 16a) or version 3 (stage15 a/b) chemistry and sequenced on a single HiSeqX (Illumina) lane to generate 400-450 million paired-end 150 bp reads (Supplementary Table 4).
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bioRxiv - Cancer Biology 2020Quote: ... libraries for mRNA sequencing were prepared using the QuantSeq 3’ mRNA FWD kit (Lexogen) prior to sequencing on the Nextseq500 (High output, SR75, Illumina). On average 7.0 ± 1.9M high-quality reads were generated per samples ...
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bioRxiv - Immunology 2020Quote: ... Single cell RNA libraries were prepared according to the 10x Genomics Chromium Single Cell 3’ Reagent Kits v2 User Guide and sequenced on a HiSeq4000 (Illumina).
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bioRxiv - Genomics 2021Quote: ... cells and CD14+ monocytes were generated using the SureCell WTA 3′ Library Prep Kit for the ddSEQ System (20014280, Illumina) with the following modifications ...
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bioRxiv - Physiology 2021Quote: Sodium-bisulfite treated DNA of nine samples (6 dHT vehicle treated and 3 dHT drug treated) was subjected to measure global DNAm by Infinium Mouse methylation BeadChip array (Illumina) according to manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... single-cell barcoded cDNA libraries were generated with 10X Chromium preparation system using the Chromium Single Cell 3’ Library & Gel Bead Kit v3 and sequenced by Illumina HiSeq ...
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bioRxiv - Microbiology 2020Quote: ... The obtained partitions were further processed using the Chromium Single Cell 3’ Reagent Kit (version 2, 10x Genomics) to generate Nextera XT sequencing libraries that were sequenced on a HiSeq3000 (Illumina), using a single flow cell lane for each library.
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bioRxiv - Evolutionary Biology 2020Quote: ... and mate-pair libraries of 3 kb insert size were prepared using the Nextera Mate Pair Sample Preparation Kit (cat. No. FC-132-1001, Illumina). We then assessed library quality using the HS DNA Kit (Agilent ...
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bioRxiv - Developmental Biology 2021Quote: ... 25 mL of 2x NEBNext Ultra II Q5 Master Mix, 3 mL of 10 mM Universal PCR primer from Illumina, 3 mL of 10mM Index PCR primer and 9 mL of nuclease-free water and amplified for 10 cycles (98C for 10 s ...
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A tale of two transcriptomic responses in agricultural pests via host defenses and viral replicationbioRxiv - Genomics 2020Quote: ... The rRNA-depleted samples were used for TruSeq Stranded RNA Sample Prep kit to produce 5′ to 3′ strand-specific cDNA libraries (Illumina). A TruSeq SBS sequencing kit version 3 (Illumina ...
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bioRxiv - Genomics 2020Quote: RNA from a subset of human fetal (n = 3) and mouse (n = 8) cortex tissue samples was prepared with TruSeq Stranded mRNA Sample Prep Kit (Illumina) and subjected to 125bp paired-end sequencing using a HiSeq2500 (Illumina) ...
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bioRxiv - Microbiology 2021Quote: ... the two plasmid DNA mixtures were sequenced using an Illumina MiSeq instrument with reagent kit version 3 (2x 300 cycles) as recommended by the manufacturer (Illumina)) ...
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bioRxiv - Immunology 2020Quote: ... Samples were prepared using the Lexogen 3’ QuantSeq mRNA-seq Library Prep Kit FWD (Lexogen) and sequenced on the Illumina NovsSeq 6000 system (Illumina).
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bioRxiv - Immunology 2020Quote: ... Single cell libraries from sorted samples were generated using a UMI-based droplet-partitioning platform with the Chromium single cell 3’ library and gel bead kit v2 and v3 (10X Genomics) and then sequenced using a NovaSeq S2 (Illumina). Technical information relevant to the scRNA-Seq samples is found in Table S3.
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bioRxiv - Genomics 2021Quote: Single-cell libraries were prepared from 12,000 cells per time point (Figure 1A) using the SureCell WTA 3’ Library Prep Kit for the ddSEQ System (Illumina). Cells with viability >90% were collected by detaching cells from plates using Trypsin-EDTA 0.25% ...
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bioRxiv - Cell Biology 2022Quote: ... RNA from the barcoded cells was subsequently reverse-transcribed and sequencing libraries constructed with reagents from a Chromium Single Cell 3’ v3 reagent kit (10x Genomics) and sequenced with the NovaSeq system (Illumina).
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bioRxiv - Genomics 2022Quote: ... Micro-C libraries (at least 3 per each biological replicate) that passed QC criteria were pooled and paired-end sequenced on a NovaSeq6000 platform (Illumina) to >600 million read pairs per replicate (Table S1) ...
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bioRxiv - Immunology 2022Quote: Samples were prepared using a Chromium Single Cell 3’ Reagent Kit (10x Genomics) and run on a NovaSeq S4-200 cell (Illumina).
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bioRxiv - Neuroscience 2022Quote: ... cells were harvested and prepared with 10X Chromium Single Cell 3’ Reagents V3 and sequenced on a NovaSeq 6000 (Illumina) using a S2 flow cell at 2 × 100bp ...
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bioRxiv - Developmental Biology 2022Quote: ... Indexed libraries were prepared using the Chromium Single Cell 3’ Library & Gel Bead Kit v2 (10X Genomics; PN-120237) and sequenced on a NovaSeq 6000 (Illumina) to obtain a sequencing depth of 1.50E+08 paired-end reads per library ...
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bioRxiv - Cell Biology 2022Quote: ... 13177 cells from a mixture of 3 diabetics) were captured for scRNA-seq library preparation and paired-end sequencing using the NovaSeq 6000 SP100 (Illumina) carried out at the GCTU of Queen’s University Belfast ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... RNA libraries were prepared using QuantSeq 3’-mRNA Library Prep Kit (Lexogen) and RNA-seq was performed with the NovaSeq 6000 (Illumina).