Labshake search
Citations for Illumina :
1901 - 1950 of 2025 citations for Apolipoprotein B mRNA Editing Enzyme Catalytic Subunit 3G APOBEC3G Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2020Quote: We generated unstranded RNA-seq libraries using the Illumina TruSeq Total RNA kit according to the manufacturer’s instructions using the total mRNA collected from all 12 individuals (Illumina, San Diego, CA, USA). We sequenced RNA-seq libraries at the University of Chicago Genomics Core facility using the single-end 50 bp protocol on one lane of the Illumina HiSeq 4000 machine ...
-
bioRxiv - Immunology 2020Quote: ... The cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA). The average insert size for the paired-end libraries was 300 bp (±50 bp) ...
-
bioRxiv - Genomics 2020Quote: ... A total of 300 ng of RNA per sample was used to prepare cDNA libraries with a TruSeq Stranded mRNA LT Kit (Illumina, San Diego, USA). All libraries were sequenced on the HiSeq 2500 platform (Illumina ...
-
bioRxiv - Genomics 2022Quote: ... 3 μg of total RNA from each sample was converted into cDNA using the TruSeq Stranded mRNA LT Sample Prep Kit (Catalog No. RS-122-2101, Illumina, San Diego, CA) to construct cDNA libraries ...
-
bioRxiv - Plant Biology 2022Quote: Sequencing libraries were generated with 300 ng to 1 μg of RNA using the TruSeq Stranded mRNA Sample Preparation Kit (Illumina, San Diego, CA). The libraries were assessed for size and purity using the Agilent 2100 Bioanalyzer and quantitated by qPCR using a KAPA Biosystems Library Quantitation kit (Roche ...
-
bioRxiv - Microbiology 2022Quote: ... and 100 ng of the enriched mRNA used to generate cDNA libraries with unique barcodes (Next UltraII Directional RNA Library Prep kit for Illumina, New England Biolabs). The cDNA libraries were pooled and subjected to RNA-Seq analysis at the UF-ICBR using the Illumina NextSeq 500 platform ...
-
bioRxiv - Neuroscience 2022Quote: ... The RNASeq libraries were prepared from total RNA using the TruSeq®Stranded mRNA LT Sample Prep Kit (Illumina Inc., Rev.E, October 2013). Briefly ...
-
bioRxiv - Immunology 2022Quote: Approximately 200 ng total RNA was used to generate single-end cDNA libraries using TruSeq stranded mRNA Library Prep (Illumina, San Diego, CA). The libraries were sequenced using a NovaSeq 6000 Sequencing System in 100 base single reads and all samples had around 120M reads ...
-
bioRxiv - Microbiology 2022Quote: ... greater than 8.0 were used to generate RNA sequencing libraries using the TruSeq Stranded mRNA Sample Prep Kit with TruSeq Unique Dual Indexes (Illumina, San Diego, CA). Samples were processed following manufacturer’s instructions ...
-
bioRxiv - Genomics 2022Quote: Illumina RNAseq libraries were prepared for both Porites lobata and Pocillopora meandrina using the TruSeq Stranded mRNA kit (Illumina, San Diego, CA, USA), according to the manufacturer’s protocol starting with 500 ng total RNA ...
-
bioRxiv - Cell Biology 2023Quote: ... mRNA-seq libraries were generated using NEB Ultra II stranded mRNA chemistry (NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®), as per the manufacturers protocol with the following settings ...
-
bioRxiv - Neuroscience 2022Quote: ... Illumina TruSeq mRNA libraries were sequenced at a resolution of 50 base single reads on a HiSeq2000 using V3 chemistry (Illumina Inc., San Diego). Resulting reads were trimmed using Cutadapt (version 1.12 ...
-
bioRxiv - Neuroscience 2022Quote: ... Library preparation was performed with 50ng of RNA using the TruSeq PolyA+ Stranded mRNA Library Prep Kit High Throughput (Illumina, RS-122-2103). Libraries were quality-checked on a Fragment Analyzer (Advanced Analytical ...
-
bioRxiv - Microbiology 2023Quote: ... RNA samples with RQNs ranging from 8 to 10 were used for RNA library preparation with the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA). Briefly ...
-
bioRxiv - Microbiology 2023Quote: The sequencing library was prepared using the TruSeq Stranded mRNA Sample Prep Kit and sequenced on NovaSeq 6000 (Illumina, San Diego, CA, USA), yielding more than 6G bases of sequence for each sample ...
-
bioRxiv - Cancer Biology 2023Quote: ... Illumina sequencing libraries with 6-bp single indices were constructed from 1 μg total RNA using the TruSeq Stranded mRNA LT kit (Illumina, RS-122-2101). The resulting library was validated using the Standard Sensitivity NGS Fragment Analysis Kit (AATI ...
-
bioRxiv - Developmental Biology 2023Quote: ... Sequencing libraries were prepared at the Genomics Technology Core at the University of Missouri using the TruSeq Stranded mRNA kit (Illumina, San Diego, CA). Approximately 35 million pair-end sequences of 100 nucleotides long were produced per sample in a NovaSeq6000 instrument (Illumina ...
-
bioRxiv - Molecular Biology 2023Quote: ... X-ray and Fe-ions irradiated RNA samples were treated as follows: RNA libraries were prepared using the TruSeq Stranded mRNA protocol (Illumina, San Diego, USA). Libraries were purified and evaluated using an Agilent 2100 bioanalyzer ...
-
bioRxiv - Cancer Biology 2023Quote: ... A-tailing as well as adaptor ligation and library enrichment were performed as described in the TruSeq Stranded mRNA library preparation kit (Illumina: RS-122-2101) according to the manufacturers manual ...
-
bioRxiv - Physiology 2023Quote: ... RNA samples with RQNs ranging from 8 to 10 were used for RNA library preparation with the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA). Briefly ...
-
bioRxiv - Systems Biology 2023Quote: ... greater than 8.0 were used to generate RNA sequencing libraries using the TruSeq Stranded mRNA Sample Prep Kit with TruSeq Unique Dual Indexes (Illumina, San Diego, CA). Samples were processed following manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... We produced libraries for 31-mRNA sequencing using 200 ng of purified RNA per sample processed with a TruSeq Stranded RNA Kit (Illumina, San Diego, CA) according to the manufacturer’s protocol ...
-
bioRxiv - Genetics 2023Quote: ... Poly-A selection and cDNA synthesis were performed using the TruSeq Stranded mRNA kit as outlined by the manufacturer (Illumina RS-122-2103). All steps were automated on the Perkin Elmer Sciclone NGSx Workstation ...
-
bioRxiv - Developmental Biology 2023Quote: ... values qualified for a Poly-A enrichment strategy in order to generate the sequencing libraries applying the TruSeq mRNA Stranded Library Prep Kit (Illumina, Inc, California, USA). After Poly-A selection using Oligo-dT beads the mRNA was reverse-transcribed into cDNA ...
-
bioRxiv - Molecular Biology 2023Quote: ... 4 μg total RNA was used to prepare RNA-seq libraries using the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, US). Strand specific paired-end mRNA sequencing was performed on DNBSEQ platform at the Next-Generation Sequencing Core of the BGI Genomics Service (Hong Kong ...
-
bioRxiv - Molecular Biology 2023Quote: ... 200ng mRNA per sample was used for RNA-seq library preparation using the TruSeq RNA Library Prep Kit v2 (Illumina RS-122-2001) according to manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2023Quote: ... RNA samples with RQNs ranging from 8 to 10 were used for RNA library preparation with the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA). Briefly ...
-
bioRxiv - Developmental Biology 2024Quote: ... >7 passed quality control and were used for library preparation with the Illumina Stranded mRNA Library Prep Kit according to manufacturer’s instructions (Cat #20040532, Illumina Inc., San Diego, CA). This procedure includes mRNA purification and fragmentation ...
-
bioRxiv - Microbiology 2024Quote: ... DNAse-treated RNA extracts from Large RNA fractions were processed for library preparation using the Truseq Stranded mRNA sample preparation kit (Illumina, San Diego, California) according to the manufacturer’s instructions ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... we used the standard Illumina TruSeq protocol (TruSeq Stranded mRNA LP, Cat # 20020594 and TruSeq RNA Sg Idx SetB, Cat # 20020493, Illumina, San Diego, CA)) following the manufacturers recommendations and as was done previously 31 ...
-
bioRxiv - Neuroscience 2024Quote: Libraries were generated using the QuantSeq 3’ (Lexogen) mRNA protocol and subsequently sequenced using the Illumina HiSeq 4000 (Illumina; 50bp single end reads). On average 8.2M reads were obtained for each of the 10 replicate samples collected from each age (6- ...
-
bioRxiv - Cancer Biology 2024Quote: ... Library preparation and sequencing was performed at the QUT Central Analytical Research Facility (CARF) using the Illumina TruSeq Stranded mRNA Sample Prep Kit (strand-specific, polyA enriched, Illumina, San Diego, USA) with an input of 1 µg total RNA (RIN>9) ...
-
bioRxiv - Cancer Biology 2022Quote: ... but from purified CLL cells (Miltenyi B-CLL Isolation kit) and sequenced on an Illumina HiSeq 4000 (Illumina, Hayward, CA, USA). miRNA data in the form of fastq were quality checked using FastQC ...
-
bioRxiv - Genomics 2021Quote: Custom RNA capture-based libraries were prepared starting from 8.5 μL eluate for biofluid samples and 100 ng RNA for FFPE and MAQCA/B using the TruSeq RNA Exome Library Prep Kit (Illumina, USA). Library preparation happened according to the manufacturer’s protocol with some minor modifications ...
-
bioRxiv - Cell Biology 2020Quote: ... libraries for sequencing were prepared from using the TrueSeq RNA Sample Prep Kit v2-Set B (Illumina, San Diego, CA, US) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2020Quote: ... The remaining 1μl re-dissolved contents were tagmented using 0.6 μl TD Tagmentation buffer and 0.3 μl ATM Tagmentation enzyme from Nextera XT DNA Library Prep Kit (Illumina, catalog no. FC-121-1030) for 5min at 55 °C ...
-
bioRxiv - Developmental Biology 2022Quote: ... Transposition was performed directly on nuclei using 25 µl tagmentation reaction mix (Tagment DNA Buffer #15027866, Tagment DNA Enzyme #15027865 from Illumina Tagment DNA kit #20034210). Tagged DNA was subjected to PCR amplification and library indexing ...
-
bioRxiv - Genetics 2022Quote: 1184 recombinant inbred lines of the S6 generation of the Global MAGIC population were genotyped from DNA extracted from leaf tissue using Genotyping-by-Sequencing (GBS, with the enzyme ApeKI) across 14 lanes of 96-sample multiplexed sequencing (100 bp single-end Illumina, Phred+33 fastq format) as reported in Zaw et al ...
-
bioRxiv - Microbiology 2020Quote: ... One nanogram of quantified DNA was sheared enzymatically at 55°C for 5 min using the Illumina Nextera XT tagmentation enzyme (Illumina, San Diego, CA, USA). Tagmented DNA fragments were enriched by 10 cycles of PCR amplification using PCR master mix and primers with the index from Illumina ...
-
bioRxiv - Plant Biology 2023Quote: ... RADseq library was prepared with HindIII and NlaIII restriction enzymes for DNA digestion and sequenced using Illumina NovaSeq 6000 (Illumina, San Diego, CA, USA). Raw sequence data is available on NCBI SRA under the project number PRJNA997794 ...
-
bioRxiv - Immunology 2024Quote: ... transposition reaction was done with 350 rpm mixing and using the transposase from the Illumina Tagment DNA TDE1 Enzyme and Buffer Kit (Illumina, San Diego, CA, USA). Libraries were quantified using KAPA Library Quantification kit (Roche ...
-
bioRxiv - Genomics 2020Quote: ... Individual libraries for each of the analyzed bovines (N=16) were processed using the TruSeq Stranded mRNA Preparation kit (Illumina, San Diego, CA, USA) according to manufacturer’s instructions ...
-
bioRxiv - Physiology 2022Quote: RNA-seq Expression Analysis: RNA-seq libraries were prepared from total RNA using the TruSeq Stranded mRNA kit (Illumina, Inc., San Diego, CA, USA). Library size distribution was validated using an Agilent 4200 TapeStation (Agilent Technologies ...
-
bioRxiv - Genomics 2022Quote: RNA-Seq library preparations were carried out from 500ng to 2000ng of total RNA using the TruSeq Stranded mRNA kit (Illumina, San Diego, CA, USA), which allows mRNA strand orientation (sequence reads occur in the same orientation as antisense RNA) ...
-
bioRxiv - Developmental Biology 2019Quote: ... RNA-seq libraries were generated from 200-300 ng total RNA with the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2019Quote: ... RNA-Seq libraries were made from 1 µg total RNA per tissue sample using TruSeq stranded mRNA Library Prep Kit (Illumina, kit cat. no. 20020597) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2019Quote: ... Extracted single cell RNA from identified neurons from the STG was used to generate cDNA libraries using TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, California, USA). Libraries were sequenced in a paired-end 150 bp (2×150bp ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... Transcriptome libraries were then constructed using poly-A selection with the TruSeq Stranded mRNA Library Prep Kit (Cat. No. RS-122-2101, Illumina, San Diego, CA, USA). In brief ...
-
bioRxiv - Cancer Biology 2020Quote: Libraries for cluster generation and DNA sequencing were prepared following an adapted method from Fisher et al49 using Illumina TruSeq Stranded mRNA LT Kit (Illumina, San Diego, CA, USA). Quality and quantity of the DNA libraries was assessed on a Agilent 2200 Tapestation (D1000 screentape ...
-
bioRxiv - Cancer Biology 2020Quote: ... converted to cDNA and ligated to sequencing adaptors containing indexes using the Illumina TruSeq stranded mRNA sample preparation kit (Cat. #RS-122-2101, Illumina, Inc., San Diego, CA). The molar concentrations of the indexed libraries were measured using the 2100 Agilent Bioanalyzer (Agilent Technologies ...