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Citations for Illumina :
1901 - 1950 of 2411 citations for 7 3 2 3 5 dihydroxyphenyl 2 hydroxyethyl amino propyl 3 7 dihydro 1 3 dimethyl 1H purine 2 6 dione monohydrochloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: ... Strand-specific library preparation (one library per sample) and paired-end (150 + 150 bp) Illumina sequencing (Illumina HiSeq 3000, 5 lanes) was then performed at the Finnish Functional Genomics Center (FFCG) ...
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bioRxiv - Genetics 2021Quote: 5× HOT FIREPol® EvaGreen® qPCR Mix Plus with ROX (Solis Biodyne) and an Eco Real-Time PCR System (Illumina) were used for qPCR ...
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bioRxiv - Genomics 2020Quote: ... the fragmented amplicons were cleaned-up and amplified by 5 cycles of PCR using specific index adapters for Illumina sequencing (Nextera™ DNA CD Indexes, Illumina) (Supplementary Figure 1B) ...
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bioRxiv - Developmental Biology 2022Quote: ... After centrifugation supernatant was used for RNA cytosolic fraction by adding to 1 ml of TRIZOL and isolated nuclei of CMs were incubated in 30 µl and of EC in 10 µl transposase mixture with 5% Nextera Tagment DNA Enzyme TDE (Illumina #15027916) in transposition buffer (20 mM Tris-HCl pH 7.6 ...
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bioRxiv - Molecular Biology 2022Quote: ... Nextera libraries (5 replicates) were constructed from 0.8 ng of pre-amplified cleaned up cDNA using Nextera XT Kit (Illumina, Eindhoven, Netherlands). Index PCR was carried out using the custom P5 primer (P5NEXTPT5 ...
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bioRxiv - Cell Biology 2024Quote: ... from islets was added to 50 μM first-strand RT primer (the sequence at the 5’ portion corresponds to primer P7 on the Illumina flowcell), 10 mM dNTP mix ...
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bioRxiv - Cell Biology 2024Quote: ... The second-strand cDNA was synthesized by adding 100 μM primer (the specific sequence at the 5′ portion corresponds to the primer for sequencing on the Illumina flowcell), 10× Taq DNA Polymerase Buffer ...
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bioRxiv - Microbiology 2024Quote: ... Sequencing libraries were prepared from 700 ng total RNA using the TruSeq stranded mRNA library preparation kit (Cat# 20020594/5, Illumina Inc.) including polyA selection ...
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bioRxiv - Microbiology 2023Quote: ... Amplicon libraries were mixed with 5% PhiX and sequenced with MiSeq reagent kits v2 500 cycles (Illumina, San Diego, California, USA). A blank extraction kit control ...
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bioRxiv - Genomics 2023Quote: A 96-well plate pre-loaded with 5 μl of 500 nM pre-indexed Tn5 transposase per well (iTSM plate, kind gift of Illumina Inc.) was used to multiplex samples and perform barcoded transposition ...
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bioRxiv - Genomics 2023Quote: ... we pooled these libraries with 5% of bulk ATAC libraries (from an unrelated experiment) and sequenced them on a NextSeq 550 Sequencer (Illumina Inc.) using a High Output Kit with following cycles ...
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bioRxiv - Neuroscience 2023Quote: ... and a duplicate unrelated control sample was diluted to 5 ng/µl in low TE provided in AmpliSeq Library PLUS (384 Reactions) kit (Illumina, 20019103). AmpliSeq was carried out following the manufacturer’s protocol (document 1000000036408v07) ...
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bioRxiv - Immunology 2024Quote: ... The 5’ expression library and the V(D)J library were sequenced with NovaSeq 6000 S1 (100 cycles) (Illumina, cat. 20012865).
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bioRxiv - Microbiology 2023Quote: ... 1 μl of forward and 1 μl of reverse 25 μM PCR primers (Illumina), and 0.5 μl of Phusion high-fidelity DNA polymerase (New England Biolabs) ...
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bioRxiv - Neuroscience 2023Quote: ... collected with the magnet and resuspended in 24ul of Tagmentation Mix (1:1 Illumina 2× Tagmentation buffer ...
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bioRxiv - Genetics 2019Quote: ... 1% PhyX spike-in (Illumina) was included ...
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bioRxiv - Immunology 2021Quote: ... Group 1 (The Netherlands, Illumina), Group 2 (France ...
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bioRxiv - Microbiology 2021Quote: ... 1×75 cycles (Illumina, USA). Image analysis was performed in real time by the NextSeq Control Software and Real Time Analysis running on the instrument computer ...
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bioRxiv - Systems Biology 2022Quote: ... 1% PhyX spike-in (Illumina) was added then pooled ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were lysed for 5 min followed by transposase reaction and library amplification using Nextera DNA Library Prep Kit (Illumina, California, USA). Libraries were then size-selected (240-360 bp ...
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bioRxiv - Cancer Biology 2022Quote: ... Single-end sequencing (5’ end, 75 bp) was performed at the Cornell Biotechnology Research Center using the NextSeq500 (Illumina, San Diego, CA) platform ...
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bioRxiv - Genomics 2021Quote: ... ChRO-seq libraries were sequenced (5′ single end; single-end 75×) using the NextSeq500 high-throughput sequencing system (Illumina, San Diego, CA) at the Cornell University Biotechnology Resource Center.
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bioRxiv - Genomics 2022Quote: ... ChRO-seq libraries were sequenced (5′ single end; single-end 75×) using the NextSeq500 high-throughput sequencing system (Illumina, San Diego, CA) at the Cornell University Biotechnology Resource Center ...
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Activation of XBP1s attenuates disease severity in models of proteotoxic Charcot-Marie-Tooth type 1BbioRxiv - Neuroscience 2024Quote: ... S63del and S63del/Xbp1SC-KO mice using the Zymo RNA Clean & Concentrator-5 (#R1013) and sent to Genewiz (South Plainfield, NJ) for library preparation after PolyA selection and Illumina sequencing (Illumina HiSeq 2x150bp). Illumina sequencing data were mapped to the GRCm38/mm10 genome using the STAR aligner.35 Data were analyzed using DESeq2 to determine differentially regulated genes (p-value < 0,5).36 RNA-seq data are deposited in NCBI GEO under accession number GSE252089.
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bioRxiv - Physiology 2024Quote: ... tagmented DNA was amplified by PCR in a reaction mix (5 µL DNA, 2.5 µL of 25 μM forward primer (Nextera/Illumina i5 adaptors (Illumina)) ...
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bioRxiv - Genomics 2023Quote: ... Trimmomatic version 0.39 was employed to trim reads after a quality drop below a mean of Q15 in a window of 5 nucleotides and keeping only filtered reads longer than 15 nucleotides (Trimmomatic37: a flexible trimmer for Illumina sequence data). Reads were aligned versus Ensembl mouse genome version mm10 (Ensembl release 101 ...
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bioRxiv - Genomics 2024Quote: ... Libraries with 5% PhiX spike-in were sequenced on an Illumina NextSeq 2000 sequencer with P3 200 cycles kits (Illumina cat. #20040560) as paired-end ...
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bioRxiv - Genomics 2019Quote: ... the nuclei pellets were resuspended in transposase Master Mix (1.25 μl 10x TD buffer, 5 μl H2O and 6.5 μl of Tn5: Illumina Nextera Kit; FC-121-1031) and incubated for 30 minutes at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... 50bp RNA-seq of IP fractions and total homogenate from 5 replicates per cell type (20 samples total) was performed on a single lane of a HiSeq 3000 (Illumina, San Diego, California), yielding ∼350M reads ...
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bioRxiv - Neuroscience 2020Quote: ... indexing and amplification of the transposed DNA samples was performed by combining 10 µL of transposed DNA with the following: 5 µL of the Nextera i5 and i7 indexed amplification primers (Nextera Index Kit, Illumina, FC-121-1011), 25 μl of the NEBNext High-Fidelity 2x PCR Master Mix (New England Biolabs ...
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bioRxiv - Evolutionary Biology 2022Quote: ... complete cytb was amplified using two different strategies: in 5 overlapping fragments (302 to 375 bp long) to perform short-read sequencing (Illumina or Ion Torrent), and in a single fragment (1457 bp long ...
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bioRxiv - Molecular Biology 2019Quote: ... 10 TLX1/TLX3 and 5 HOXA) was performed using the TruSeq Stranded Total RNA (w/RiboZero Gold) sample prep kit (Illumina, RS-122-2301), involving depletion of ribosomal (rRNA ...
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bioRxiv - Genomics 2021Quote: ... RNA-seq libraries were prepared from 500 ng total RNA using the TruSeq stranded mRNA library preparation kit (Illumina Inc, Cat# 20020594/5) including polyA selection ...
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bioRxiv - Microbiology 2021Quote: ... [34] was used to compute alignments with a minimum size of 5 kbp (--min-alignment 5000) considering as reference short-read contigs assembled with Velvet (section Collection of Illumina short-read assemblies) from the same isolate against Unicycler ...
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bioRxiv - Immunology 2020Quote: ... PCR amplification and index incorporation were performed in a 50 μl reaction containing 5 μl of forward and reverse index primers (Illumina Nextera Index Kit), 15 μl NPM ...
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bioRxiv - Genomics 2020Quote: ... Individual cells were sequenced to a mean depth of ~1.5 million 38 bp paired-end reads on an Illumina NextSeq 500 instrument with 75 cycle high output kits (Illumina TG-160-2005).
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bioRxiv - Microbiology 2024Quote: Total RNA of 5 μg was used to construct strand-specific RNA-sequencing libraries using the TruSeq RNA sample preparation Kit from Illumina (San Diego, CA). Briefly ...
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bioRxiv - Genomics 2023Quote: ... about 1.5 ng purified pre-amplified cDNA sample was fragmented in four 20 μl tagmentation mix (10 μl 2x TD buffer, 5 μl Nextera XT (Illumina, FC-131-1096), and 5 μl cDNA sample ...
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bioRxiv - Cell Biology 2024Quote: ... library was generated for 10 plasma exosome samples (n=5, young and old individuals each) using a propriety Illumina Truseq™ Small RNA Preparation kit (Illumina, # RS-930), according to the manufacturers’ guide ...
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bioRxiv - Genomics 2024Quote: ... Miniaturization involved testing libraries at 1/6th (IDT mini) or 1/8th (Roche mini, Illumina mini) of reaction volume tailoring input DNA to each kit between 24-45 ng total (Table 2) ...
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bioRxiv - Neuroscience 2021Quote: ... 1 μtagment DNA enzyme (Nextera, Illumina), l nuclease free water ...
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bioRxiv - Genetics 2019Quote: ... supplemented with 1 μl Tn5 (Illumina). Samples were then reverse cross-linked using the iDeal® ChIP-seq kit for histones according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... 1 μl of transposase (Nextera, Illumina) was added and samples were incubated at 37°C for 10 minutes followed by two washes with low-salt buffer ...
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bioRxiv - Immunology 2020Quote: ... cells were lysed in lysis buffer for 1 minute and transposed with Tagment DNA Enzyme 1 (Illumina) for 30 minutes ...
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bioRxiv - Genomics 2020Quote: ... The pool was denatured with sodium hydroxide according to Illumina recommendation and spiked with 5% PhiX (PhiX control v3 Illumina Catalogue FC-110-3001) before loading (Fig 1).
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bioRxiv - Cancer Biology 2019Quote: ... To this end shotgun libraries were generated from 5-10ng of plasma DNA using the TruSeq DNA Nano Sample Preparation Kit (Illumina, San Diego, CA, USA) as previously described[35] ...
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bioRxiv - Genomics 2019Quote: ... A mate pair library with an insert size of 5 kb was created with the Nextera Mate Pair Sample Prep Kit (Illumina, San Diego, CA, USA). The paired-end and mate pair libraries were sequenced on an Illumina MiSeq machine ...
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bioRxiv - Microbiology 2020Quote: ... One nanogram of quantified DNA was sheared enzymatically at 55°C for 5 min using the Illumina Nextera XT tagmentation enzyme (Illumina, San Diego, CA, USA). Tagmented DNA fragments were enriched by 10 cycles of PCR amplification using PCR master mix and primers with the index from Illumina ...
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bioRxiv - Genomics 2020Quote: Small RNA libraries were prepared starting from 5 µL DNase treated and spike-in supplemented RNA eluate using a TruSeq Small RNA Library Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer’s protocol with two minor modifications(1) ...
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bioRxiv - Plant Biology 2020Quote: ... on Thermo Fisher Scientific’s Quantstudio 5 before multiplex pooling (13-16 samples per lane in equal representation) and sequencing on the NextSeq500 platform (Illumina, San Diego, California, USA) paired-end 2×150 bp High Output.