Labshake search
Citations for Illumina :
1751 - 1800 of 9668 citations for Cow Four And A Half LIM Domains Protein 2 FHL2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The supernatants were carefully removed and the pellets were re-suspended in 25 μL of transposase reaction mix (12.5 μL 2×TD buffer, 1.25 μL TN5 transposase (Illumina) and 11.25 μL nuclease-free water) ...
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bioRxiv - Microbiology 2022Quote: ... The quality of paired-end reads obtained from MiSeq sequencing was analyzed by using Qiagen CLC Genomics Workbench 22.0.1 and the Identify ARTIC V3 SARS-CoV-2 Low Frequency and Shared Variants (Illumina) workflow was used in genetic variant analyses ...
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bioRxiv - Neuroscience 2023Quote: ... Libraries were sequenced at low-pass (2x 50bp paired end) targeting 2 million reads on a NextSeq (Illumina) instrument ...
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bioRxiv - Plant Biology 2023Quote: ... Amplicons were sequenced (2×250 bp) on a NovaSeq 6000 SP flow cell (Illumina, San Diego, CA, USA). Each “batch” of 96 samples (n=6 ...
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bioRxiv - Plant Biology 2024Quote: ... The final pool was run at 750 pM with 2% PhiX control library on a NextSeq 2000 (Illumina) with a P3 300 cycle kit ...
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bioRxiv - Genomics 2023Quote: ... The nuclei pellets were resuspended in 50μl of transposition buffer (25μl of 2× TD buffer, 22.5μl of distilled water, 2.5 μl of Illumina Tn5 transposase) and incubated at 37 °C for 30 min with shaking at 1000 rpm for fragmentation ...
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bioRxiv - Microbiology 2024Quote: ... The PCR products were then sent to Biofidal sequencing company for purification and 2×300bp Miseq sequencing (Illumina). A total of 12,836,921 and 14,248,382 reads were obtained from 16S and 18S rDNA respectively and were demultiplexed using the Mothur pipeline (69 ...
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bioRxiv - Microbiology 2020Quote: ... Library construction and sequencing were conducted using the Illumina Nextera NGS Library Prep Kit or the Illumina Nextera XT DNA Library Prep Kit (Illumina, San Diego, CA). Libraries were sequenced on an Illumina NextSeq with 150bp paired-end reads ...
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bioRxiv - Genomics 2020Quote: ... The resulting nuclei were resuspended in 50µl of transposition reaction buffer (25µl of 2x TD buffer from the Nextera kit, Illumina; 2.5µl of Tn5 transposase enzyme from the Nextera kit, Illumina; 22.5µl of nuclease free water), and incubated at 37°C for 90 min for chromatin tagmentation ...
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bioRxiv - Microbiology 2019Quote: ... One to two ng of DNA per sample were taken for library preparation using the Nextera XT DNA Library Preparation Kit and uniquely indexed samples using the Nextera XT Index Kit were pooled and sequenced together (both Illumina, San Diego, California). Pooled libraries were sequenced with paired-end reads (2×150-250 ...
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bioRxiv - Plant Biology 2021Quote: ... A paired-end library and four mate libraries were constructed using a TruSeq DNA PCR-Free Library Prep Kit and a Nextera Mate Pair Sample Preparation Kit (Illumina, San Diego, CA), respectively ...
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bioRxiv - Cell Biology 2021Quote: ... Cluster generation and sequencing involved application of the TruSeq SR Cluster Kit v4-cBot-HS or TruSeq PE Cluster Kit v4-cBot-HS (Illumina, Inc, California, USA) using 8 pM of pooled normalized libraries on the cBOT ...
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bioRxiv - Immunology 2022Quote: ... purified with NucleoSpin Gel and PCR Clean-up kit (Quiagen, cat. 740609.50) and sequenced on the MiSeq platform with a MiSeq 600 cycle v3 kit (Illumina, cat. MS-102-3003).
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bioRxiv - Microbiology 2022Quote: ... Libraries of 3 nM and above concentration were loaded on to NovaSeq 6000 S4 Reagent Kit using a paired-end 150 kit (20012866; Illumina, San Diego, CA). Each sample was sequenced to a depth of at least 19.5 million reads ...
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bioRxiv - Cell Biology 2022Quote: ... 1,500ng of RNA at 50ng/μl was processed using Ribo-Zero Gold purification (QIAseq FastSelect -rRNA HMR Kit #334387) and NEBNext® Ultra™ II RNA Library Prep Kit (Illumina #E7770L). cDNA was sequenced using paired-end 150bp chemistry on a HiSeq 4000 instrument (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... Libraries were prepared using the New England Biolabs NEBNext rRNA depletion kit (Bacteria) and NEBNext Ultra II Directional RNA library prep kit (Illumina, San Diego, CA). The libraries were quantified by KAPA qPCR library quantitation assays and sequenced paired-end using MiSeq platform (Illumina ...
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bioRxiv - Genetics 2019Quote: Libraries for the pENTR and pDEST (pTrypLib) ORF plasmid libraries were prepared for next generation Illumina sequencing using tagmentation kits from Illumina (Nextera XT kit) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... Tagmentation and library preparation was done using the Nextera XT DNA Library Prep kit and sequenced using the NextSeq 500/550 High Output Kit v2 (75 cycles) 400 million reads (Illumina, #FC-404-2005) on Illumina NextSeq 500 platform ...
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bioRxiv - Immunology 2019Quote: ... RNA-seq libraries were prepared using the TruSeq Stranded Total RNA kit paired with the Ribo-Zero rRNA removal kit (Illumina, San Diego, CA). Libraries were sequenced on an Illumina HiSeq 4000 instrument ...
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bioRxiv - Microbiology 2019Quote: Sample dilution and library construction were performed with halved reactions according to the manufacturer’s instructions for the three library preparation kits evaluated: Nextera XT DNA Library Prep Kit (Illumina, San Diego, CA USA) and KAPA HyperPlus Kit (Roche ...
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bioRxiv - Neuroscience 2019Quote: ... Tissue RNA-seq was generated using the TruSeq RNA Sample Preparation Kit v2 and Ribo-Zero rRNA removal kit (Illumina, San Diego, CA). For RNA-seq data processing ...
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bioRxiv - Immunology 2019Quote: ... before tagmentation and PCR amplification with index primers using the Nextera XT DNA SMP Prep Kit and Nextera XT IDX Kit (Illumina, San Diego, CA). After cleanup with Agencourt AMPure XP beads (Beckman Coulter ...
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bioRxiv - Immunology 2021Quote: ... Libraries were quantified sing Qubit High-Sensitivity DNA kit and preparation kit and libraries were constructed using Nextera XT DNA tagmentation (Illumina FC-131-1096) using 800 pg of pooled cDNA library as input using index primers with format as in Gierahn et al [46] ...
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bioRxiv - Molecular Biology 2022Quote: ... Purified multiplexed libraries were diluted to 2nM concentration (calculated based on the Qubit dsDNA HS Assay Kit) for sequencing using NextSeq 500/550 v2.5 Kit (Illumina, 35bp paired-end reads). Finally ...
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bioRxiv - Molecular Biology 2022Quote: ... prior to mixing of multiplexed libraries diluted to 2nM concentration (calculated based the on Qubit dsDNA HS Assay Kit) for sequencing using the NextSeq 500/550 v2.5 kit (Illumina, 75bp single-end reads). Finally ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA-seq libraries were generated from 500 ng of total RNA using the TruSeq Stranded mRNA Library Prep Kit and TruSeq RNA Single Indexes kits A and B (Illumina, San Diego, CA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... 20-100 ng genomic DNA was used to prepare WGS libraries using the Illumina DNA Prep Kit (formerly known as Illumina Nextera Flex Kit) using a modified protocol that utilized 5x reduced quantities of tagmentation reagents per prep ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA-Seq libraries were generated from 100 to 300 ng of RNA using TruSeq Stranded Total RNA Library Prep Gold kit and TruSeq RNA Single Indexes kits A and B (Illumina, San Diego, CA), according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... The DNA concentration was measured using the Qubit dsDNA BR assay kit and sequenced using a HiSeq SBS v4 50 cycle kit (Illumina FC-401-4002) and HiSeq SR Cluster Kit v4 (Illumina GD-401-4001 ...
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bioRxiv - Microbiology 2023Quote: ... Library preparation was performed using an Illumina Nextera DNA Flex Library Prep Kit and multiplexing at 96-plex on a NextSeq 500 System using the Mid-Output Kit (Illumina, San Diego, USA). Resulting fastq files underwent a quality check ...
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bioRxiv - Cell Biology 2023Quote: ... Library preparation is performed with or without amplification (Nextera® DNA Flex Library Prep kit or TruSeq® DNA PCR-Free kit, ILLUMINA®), either through automation (MICROLAB Star® ...
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bioRxiv - Molecular Biology 2023Quote: Multiplex sequencing libraries were generated from 500 ng of total RNA (per library) using Illumina’s TruSeq sample prep kit and multiplexing sample preparation oligonucleotide kit (Illumina Inc., San Diego, CA) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... The DNA concentration was measured using the Qubit dsDNA BR assay kit and sequenced using a HiSeq SBS v4 50 cycle kit (Illumina FC-401-4002) and HiSeq SR Cluster Kit v4 (Illumina GD-401-4001 ...
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bioRxiv - Microbiology 2023Quote: ... We prepared DNA libraries employing the Nextera XT library preparation kit and the Nextera XT v2 index kit (Illumina, San Diego, CA, USA). The library was sequenced on a HiSeq4000 (Illumina ...
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bioRxiv - Neuroscience 2024Quote: ... RNA-Seq libraries were generated from 200 ng of total RNA using TruSeq Stranded mRNA Library Prep Kit and TruSeq RNA Single Indexes kits A and B (Illumina, San Diego, CA), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Total RNA-Seq libraries were generated from 700 ng of total RNA using TruSeq Stranded Total RNA Library Prep Gold kit and TruSeq RNA Single Indexes kits A and B (Illumina, San Diego, USA), according to manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... and the final sequencing library was amplified utilizing the KAPA HiFi HotStart Uracil+ ReadyMix PCR Kit along with index primers from the Nextera XT Index Kit (Illumina, FC-131-1001). The amplification protocol involved an initial step at 98°C for 45 seconds ...
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bioRxiv - Genomics 2024Quote: ... Total RNA-seq libraries were then generated using TruSeq Stranded mRNA Library Prep kit and TruSeq RNA Single Indexes kits A and B (Illumina, San Diego, CA) omitting the poly-A selection step and starting from the fragmentation step ...
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bioRxiv - Microbiology 2020Quote: ... Illumina shotgun libraries were prepared using the Nextera XT DNA Sample Preparation Kit and subsequently sequenced on a MiSeq system with the reagent kit v3 with 600 cycles (Illumina, San Diego, CA, USA) as recommended by the manufacturer ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA-Seq libraries were made from 1 µg total RNA per tissue sample using TruSeq stranded mRNA Library Prep Kit (Illumina, kit cat. no. 20020597) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA library synthesis and capture were performed using the Illumina TruSeq RNA Access Library Prep kit (now known as Illumina TruSeq RNA Exome kit). Amplified libraries were quantified using an automated PicoGreen assay ...
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bioRxiv - Genetics 2022Quote: ... Retinal cDNA libraries were created by the Iowa Institute of Human Genetics Genomics Division using the TruSeq Stranded mRNA Library Prep Kit and the TruSeq Small RNA Library Prep Kit (Illumina, Inc., San Diego, CA). Indexed libraries prepared from mRNA were normalized and pooled into three groups (corresponding to three biological replicates ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We then prepared dual indexed-libraries using 1ng DNA extract using the Nextera XT Library Preparation Kit and the Nextera XT Index Kit (Illumina, San Diego, CA, USA), which we pooled so individual libraries would all bring in about the same number of HSV-2 genome copies (according to the qPCR results) ...
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bioRxiv - Microbiology 2021Quote: ... The DNA library was prepared using the Nextera XT library preparation kit with the Nextera XT v2 index kit (Illumina, San Diego, CA, USA). The library fragment length was aimed at fragments with a median size of 575 bases and was assessed with the Genomic DNA ScreenTape assay with the 2200 Tape-Station system (Agilent Technologies ...
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bioRxiv - Microbiology 2021Quote: The sequencing libraries were constructed and indexed with Nextera DNA Sample Preparation kit and Index kit as recommended by the manufacturer (Illumina, San Diego, CA, USA). Paired-end sequencing was performed using a HiSeq 4000 instrument (2 x 150 bp ...
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bioRxiv - Genomics 2022Quote: ... A total of 100 ng of RNA was used as input to the mRNA sequencing library prep kit (TruSeq RNA Access Library Prep Kit by Illumina, San Diego, CA, USA) according to the protocols of the manufacturer ...
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bioRxiv - Microbiology 2022Quote: ... Illumina shotgun libraries were prepared using the Nextera XT DNA Sample Preparation Kit and subsequently sequenced on a MiSeq system with the reagent kit v3 with 600 cycles (Illumina, San Diego, CA, USA) as recommended by the manufacturer ...
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bioRxiv - Molecular Biology 2022Quote: mRNA-seq libraries were generated from 500 ng of total RNA using TruSeq® Stranded mRNA Library Prep kit and TruSeq® RNA Single Indexes kits A and B (Illumina, Cat#20020595) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Shotgun metagenomic sequencing libraries were prepared using either the Illumina Nextera XT Library Preparation Kit (39 samples) or the Illumina DNA Prep library Kit (Illumina Inc., San Diego, CA) (13 samples) ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA libraries were constructed from 1 μg/sample of total RNA using library preparation kits (NEBNext Ultra II RNA Library Prep Kit for Illumina 7770L, NEBNext Multiplex Oligos for Illumina E7335S, NEBNext Poly(A) mRNA Magnetic Isolation Module E7490L ...