Labshake search
Citations for Illumina :
1301 - 1350 of 9025 citations for Human NADH ubiquinone oxidoreductase chain 5 MT ND5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... Tagmentation and library preparation was done using the Nextera XT DNA Library Prep kit and sequenced using the NextSeq 500/550 High Output Kit v2 (75 cycles) 400 million reads (Illumina, #FC-404-2005) on Illumina NextSeq 500 platform ...
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bioRxiv - Immunology 2019Quote: ... RNA-seq libraries were prepared using the TruSeq Stranded Total RNA kit paired with the Ribo-Zero rRNA removal kit (Illumina, San Diego, CA). Libraries were sequenced on an Illumina HiSeq 4000 instrument ...
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bioRxiv - Microbiology 2019Quote: Sample dilution and library construction were performed with halved reactions according to the manufacturer’s instructions for the three library preparation kits evaluated: Nextera XT DNA Library Prep Kit (Illumina, San Diego, CA USA) and KAPA HyperPlus Kit (Roche ...
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bioRxiv - Neuroscience 2019Quote: ... Tissue RNA-seq was generated using the TruSeq RNA Sample Preparation Kit v2 and Ribo-Zero rRNA removal kit (Illumina, San Diego, CA). For RNA-seq data processing ...
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bioRxiv - Immunology 2019Quote: ... before tagmentation and PCR amplification with index primers using the Nextera XT DNA SMP Prep Kit and Nextera XT IDX Kit (Illumina, San Diego, CA). After cleanup with Agencourt AMPure XP beads (Beckman Coulter ...
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bioRxiv - Immunology 2021Quote: ... Sequencing was performed in the Human Immunology Core Facility at the University of Pennsylvania using an Illumina 2× 300-bp paired-end kit (Illumina MiSeq Reagent Kit v3, 600-cycle, Illumina MS-102-3003).
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bioRxiv - Immunology 2021Quote: ... Libraries were quantified sing Qubit High-Sensitivity DNA kit and preparation kit and libraries were constructed using Nextera XT DNA tagmentation (Illumina FC-131-1096) using 800 pg of pooled cDNA library as input using index primers with format as in Gierahn et al [46] ...
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bioRxiv - Molecular Biology 2022Quote: ... Purified multiplexed libraries were diluted to 2nM concentration (calculated based on the Qubit dsDNA HS Assay Kit) for sequencing using NextSeq 500/550 v2.5 Kit (Illumina, 35bp paired-end reads). Finally ...
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bioRxiv - Molecular Biology 2022Quote: ... prior to mixing of multiplexed libraries diluted to 2nM concentration (calculated based the on Qubit dsDNA HS Assay Kit) for sequencing using the NextSeq 500/550 v2.5 kit (Illumina, 75bp single-end reads). Finally ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA-seq libraries were generated from 500 ng of total RNA using the TruSeq Stranded mRNA Library Prep Kit and TruSeq RNA Single Indexes kits A and B (Illumina, San Diego, CA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... 20-100 ng genomic DNA was used to prepare WGS libraries using the Illumina DNA Prep Kit (formerly known as Illumina Nextera Flex Kit) using a modified protocol that utilized 5x reduced quantities of tagmentation reagents per prep ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA-Seq libraries were generated from 100 to 300 ng of RNA using TruSeq Stranded Total RNA Library Prep Gold kit and TruSeq RNA Single Indexes kits A and B (Illumina, San Diego, CA), according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... The DNA concentration was measured using the Qubit dsDNA BR assay kit and sequenced using a HiSeq SBS v4 50 cycle kit (Illumina FC-401-4002) and HiSeq SR Cluster Kit v4 (Illumina GD-401-4001 ...
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bioRxiv - Microbiology 2023Quote: ... Library preparation was performed using an Illumina Nextera DNA Flex Library Prep Kit and multiplexing at 96-plex on a NextSeq 500 System using the Mid-Output Kit (Illumina, San Diego, USA). Resulting fastq files underwent a quality check ...
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bioRxiv - Cell Biology 2023Quote: ... Library preparation is performed with or without amplification (Nextera® DNA Flex Library Prep kit or TruSeq® DNA PCR-Free kit, ILLUMINA®), either through automation (MICROLAB Star® ...
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bioRxiv - Molecular Biology 2023Quote: Multiplex sequencing libraries were generated from 500 ng of total RNA (per library) using Illumina’s TruSeq sample prep kit and multiplexing sample preparation oligonucleotide kit (Illumina Inc., San Diego, CA) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... The DNA concentration was measured using the Qubit dsDNA BR assay kit and sequenced using a HiSeq SBS v4 50 cycle kit (Illumina FC-401-4002) and HiSeq SR Cluster Kit v4 (Illumina GD-401-4001 ...
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bioRxiv - Microbiology 2023Quote: ... We prepared DNA libraries employing the Nextera XT library preparation kit and the Nextera XT v2 index kit (Illumina, San Diego, CA, USA). The library was sequenced on a HiSeq4000 (Illumina ...
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bioRxiv - Neuroscience 2024Quote: ... RNA-Seq libraries were generated from 200 ng of total RNA using TruSeq Stranded mRNA Library Prep Kit and TruSeq RNA Single Indexes kits A and B (Illumina, San Diego, CA), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Total RNA-Seq libraries were generated from 700 ng of total RNA using TruSeq Stranded Total RNA Library Prep Gold kit and TruSeq RNA Single Indexes kits A and B (Illumina, San Diego, USA), according to manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... and the final sequencing library was amplified utilizing the KAPA HiFi HotStart Uracil+ ReadyMix PCR Kit along with index primers from the Nextera XT Index Kit (Illumina, FC-131-1001). The amplification protocol involved an initial step at 98°C for 45 seconds ...
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bioRxiv - Genomics 2024Quote: ... Total RNA-seq libraries were then generated using TruSeq Stranded mRNA Library Prep kit and TruSeq RNA Single Indexes kits A and B (Illumina, San Diego, CA) omitting the poly-A selection step and starting from the fragmentation step ...
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bioRxiv - Cell Biology 2021Quote: ... Nuclei were collected by centrifugation and subjected then to transposition reaction in 1x Tagment DNA buffer containing 5% Tn5 Transposase (Illumina, 20034197), 0.1% Tween-20 ...
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bioRxiv - Genomics 2022Quote: ... pH 7.4, 5 mM MgCl2, 10% dimethyl formamide, 0.33x PBS, 0.01% digitonin, 0.1% Tween-20, 100 nM Illumina Tn5 Transposase (Illumina, 20034197)) ...
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bioRxiv - Bioengineering 2019Quote: ... incubated and room temperature for 5 minutes and diluted again to a final concentration of 4 pM using HT1 Buffer (Illumina, USA) and loaded into the MiSeq Reagent Nano Kit v2 cartridge (Illumina ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Strand-specific library preparation (one library per sample) and paired-end (150 + 150 bp) Illumina sequencing (Illumina HiSeq 3000, 5 lanes) was then performed at the Finnish Functional Genomics Center (FFCG) ...
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bioRxiv - Genetics 2021Quote: 5× HOT FIREPol® EvaGreen® qPCR Mix Plus with ROX (Solis Biodyne) and an Eco Real-Time PCR System (Illumina) were used for qPCR ...
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bioRxiv - Genomics 2020Quote: ... the fragmented amplicons were cleaned-up and amplified by 5 cycles of PCR using specific index adapters for Illumina sequencing (Nextera™ DNA CD Indexes, Illumina) (Supplementary Figure 1B) ...
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bioRxiv - Developmental Biology 2022Quote: ... After centrifugation supernatant was used for RNA cytosolic fraction by adding to 1 ml of TRIZOL and isolated nuclei of CMs were incubated in 30 µl and of EC in 10 µl transposase mixture with 5% Nextera Tagment DNA Enzyme TDE (Illumina #15027916) in transposition buffer (20 mM Tris-HCl pH 7.6 ...
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bioRxiv - Molecular Biology 2022Quote: ... 1.5 μl of PCR 1 were used as template for PCR 2 (14 cycles) and used forward primers 5’-AATGATACGGCGACCACCGAGATCTACAC-NNNNNNNN-ACACTCTTTCCCTACACGAC-3’ (compatible to Illumina i5) and reverse primers 5’-CAAGCAGAAGACGGCATACGAGAT-NNNNNNNN-GTGACTGGAGTTCAGACGTG-3’ (compatible to Illumina i7) ...
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bioRxiv - Genomics 2023Quote: A 96-well plate pre-loaded with 5 μl of 500 nM pre-indexed Tn5 transposase per well (iTSM plate, kind gift of Illumina Inc.) was used to multiplex samples and perform barcoded transposition ...
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bioRxiv - Genomics 2023Quote: ... we pooled these libraries with 5% of bulk ATAC libraries (from an unrelated experiment) and sequenced them on a NextSeq 550 Sequencer (Illumina Inc.) using a High Output Kit with following cycles ...
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bioRxiv - Neuroscience 2023Quote: ... We then randomly selected 2–3 scrambled-injected and 5–6 F0 knockout larvae for sequencing of the targeted loci (see Preparation of samples for Illumina MiSeq).
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bioRxiv - Cell Biology 2024Quote: ... from islets was added to 50 μM first-strand RT primer (the sequence at the 5’ portion corresponds to primer P7 on the Illumina flowcell), 10 mM dNTP mix ...
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bioRxiv - Cell Biology 2024Quote: ... The second-strand cDNA was synthesized by adding 100 μM primer (the specific sequence at the 5′ portion corresponds to the primer for sequencing on the Illumina flowcell), 10× Taq DNA Polymerase Buffer ...
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bioRxiv - Microbiology 2020Quote: ... Illumina shotgun libraries were prepared using the Nextera XT DNA Sample Preparation Kit and subsequently sequenced on a MiSeq system with the reagent kit v3 with 600 cycles (Illumina, San Diego, CA, USA) as recommended by the manufacturer ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA-Seq libraries were made from 1 µg total RNA per tissue sample using TruSeq stranded mRNA Library Prep Kit (Illumina, kit cat. no. 20020597) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA library synthesis and capture were performed using the Illumina TruSeq RNA Access Library Prep kit (now known as Illumina TruSeq RNA Exome kit). Amplified libraries were quantified using an automated PicoGreen assay ...
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bioRxiv - Genetics 2022Quote: ... Retinal cDNA libraries were created by the Iowa Institute of Human Genetics Genomics Division using the TruSeq Stranded mRNA Library Prep Kit and the TruSeq Small RNA Library Prep Kit (Illumina, Inc., San Diego, CA). Indexed libraries prepared from mRNA were normalized and pooled into three groups (corresponding to three biological replicates ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We then prepared dual indexed-libraries using 1ng DNA extract using the Nextera XT Library Preparation Kit and the Nextera XT Index Kit (Illumina, San Diego, CA, USA), which we pooled so individual libraries would all bring in about the same number of HSV-2 genome copies (according to the qPCR results) ...
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bioRxiv - Microbiology 2021Quote: ... The DNA library was prepared using the Nextera XT library preparation kit with the Nextera XT v2 index kit (Illumina, San Diego, CA, USA). The library fragment length was aimed at fragments with a median size of 575 bases and was assessed with the Genomic DNA ScreenTape assay with the 2200 Tape-Station system (Agilent Technologies ...
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bioRxiv - Microbiology 2021Quote: The sequencing libraries were constructed and indexed with Nextera DNA Sample Preparation kit and Index kit as recommended by the manufacturer (Illumina, San Diego, CA, USA). Paired-end sequencing was performed using a HiSeq 4000 instrument (2 x 150 bp ...
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bioRxiv - Genomics 2022Quote: ... A total of 100 ng of RNA was used as input to the mRNA sequencing library prep kit (TruSeq RNA Access Library Prep Kit by Illumina, San Diego, CA, USA) according to the protocols of the manufacturer ...
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bioRxiv - Microbiology 2022Quote: ... Illumina shotgun libraries were prepared using the Nextera XT DNA Sample Preparation Kit and subsequently sequenced on a MiSeq system with the reagent kit v3 with 600 cycles (Illumina, San Diego, CA, USA) as recommended by the manufacturer ...
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bioRxiv - Molecular Biology 2022Quote: mRNA-seq libraries were generated from 500 ng of total RNA using TruSeq® Stranded mRNA Library Prep kit and TruSeq® RNA Single Indexes kits A and B (Illumina, Cat#20020595) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Shotgun metagenomic sequencing libraries were prepared using either the Illumina Nextera XT Library Preparation Kit (39 samples) or the Illumina DNA Prep library Kit (Illumina Inc., San Diego, CA) (13 samples) ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA libraries were constructed from 1 μg/sample of total RNA using library preparation kits (NEBNext Ultra II RNA Library Prep Kit for Illumina 7770L, NEBNext Multiplex Oligos for Illumina E7335S, NEBNext Poly(A) mRNA Magnetic Isolation Module E7490L ...
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bioRxiv - Microbiology 2023Quote: ... Illumina shotgun libraries were prepared using the Nextera XT DNA Sample Preparation Kit and subsequently sequenced on a MiSeq system with the reagent kit v3 with 600 cycles (Illumina, San Diego, CA, USA) as recommended by the manufacturer ...
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bioRxiv - Microbiology 2023Quote: ... Illumina shotgun libraries were prepared using the Nextera XT DNA Sample Preparation Kit and subsequently sequenced on a MiSeq system with the reagent kit v3 with 600 cycles (Illumina, San Diego, CA, USA) as recommended by the manufacturer ...
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bioRxiv - Microbiology 2023Quote: ... Illumina shotgun libraries were prepared using the Nextera XT DNA Sample Preparation Kit and subsequently sequenced on a MiSeq system with the reagent kit v3 with 600 cycles (Illumina, San Diego, CA, USA) as recommended by the manufacturer ...