Labshake search
Citations for Illumina :
1301 - 1350 of 1539 citations for Diethyl 2 chlorothiazol 5 yl methylphosphonate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... the pool of plasmids for each library (inputs) was amplified using primers bearing sequences from Illumina (Read 1 and Read 2) and complementary to PT1 and down to Moe (Figure 1C) ...
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bioRxiv - Genomics 2024Quote: We then pooled the original and targeted libraries at a 2:1 molar concentration and sequenced them together on a NovaSeq 6000 (Illumina, 20012850) with a S1 Reagent Kit v1.5 (200 cycles ...
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bioRxiv - Genomics 2024Quote: ... we further pooled the hybrid selection into 3 “super pools.” 2) The hybrid selected libraries were sequenced on a NovaSeq 6000 (Illumina, 20012850) with a S1 Reagent Kit v1.5 (200 cycles ...
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bioRxiv - Genetics 2024Quote: ... 1 µg of total RNA was used with the TruSeq RNA Sample Prep Kit v.2 (Illumina, San Diego, CA, USA). The cDNA libraries were quantified by quantitative Real-Time PCR (qPCR) ...
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bioRxiv - Bioengineering 2024Quote: ... and 60 ng of each were pooled into a single NGS library that was sequenced on a NextSeq 2000 using a P3 2×51 cycle paired-end run (Illumina, 20040559).
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bioRxiv - Developmental Biology 2024Quote: ... Fragments were sequenced using a 150×2 paired terminal sequencing scheme on the Illumina Novase Q6000 analyzer (Illumina HiSeq sequencing platform) to obtain the sequencing results ...
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bioRxiv - Genomics 2020Quote: ... The pool was denatured with sodium hydroxide according to Illumina recommendation and spiked with 5% PhiX (PhiX control v3 Illumina Catalogue FC-110-3001) before loading (Fig 1).
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bioRxiv - Microbiology 2020Quote: ... One nanogram of quantified DNA was sheared enzymatically at 55°C for 5 min using the Illumina Nextera XT tagmentation enzyme (Illumina, San Diego, CA, USA). Tagmented DNA fragments were enriched by 10 cycles of PCR amplification using PCR master mix and primers with the index from Illumina ...
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bioRxiv - Genomics 2020Quote: Small RNA libraries were prepared starting from 5 µL DNase treated and spike-in supplemented RNA eluate using a TruSeq Small RNA Library Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer’s protocol with two minor modifications(1) ...
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bioRxiv - Plant Biology 2020Quote: ... on Thermo Fisher Scientific’s Quantstudio 5 before multiplex pooling (13-16 samples per lane in equal representation) and sequencing on the NextSeq500 platform (Illumina, San Diego, California, USA) paired-end 2×150 bp High Output.
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bioRxiv - Immunology 2021Quote: ... The V(D)J enriched library was then constructed via Chromium Single Cell 5’ Library Construction Kit (10x Genomics Cat#1000020) and libraries were sequenced in a NovaSeq™ 6000 Sequencing System (Illumina, San Diego, CA). V(D)J sequences were collapsed using Cell Ranger ...
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bioRxiv - Genomics 2020Quote: ... Each pool (2nM per library; Agilent SureSelect XT HS, n=6; Agilent SureSelect XT RNA Direct, n=6; Illumina TruSeq RNA Exome, n=5) was sequenced on one lane on the Illumina HiSeq 3000 platform in the Technology Center for Genomics and Bioinformatics at UCLA ...
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bioRxiv - Developmental Biology 2024Quote: ... Pooled libraries with unique 5′ and 3′ index adapter pairs were sequenced on an NextSeq 2000 P3 flow cell (Illumina®: San Diego, CA) to generate 66M (±12M ...
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bioRxiv - Cell Biology 2020Quote: ... as paired-end sequencing on S4 flowcell (2×150 bp reads) on Illumina NovaSeq-6000 sequencer at GR (Illumina, San Diego, CA).
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bioRxiv - Genomics 2020Quote: ... sequencing libraries of the two deeply sequenced samples were constructed with 1.8-2 μg sample DNA and the TruSeq DNA PCR-Free kit (Illumina, San Diego, CA). The remaining 74 paired-end short insert (350 nt ...
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Host transcriptomic profiling of COVID-19 patients with mild, moderate, and severe clinical outcomesbioRxiv - Genomics 2020Quote: ... as previously described.12,13,14 Libraries were then sequenced using the NovaSeq SP Reagent kit (2 × 150 cycles) from Illumina (San Diego, CA, USA) to generate a minimum of 15M reads per sample (Supplementary Table 1).
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bioRxiv - Neuroscience 2022Quote: ... The concentration was adjusted to 2 nM prior to sequencing (50 bp) on a HiSeq 2000 sequencer (Illumina, Inc., San Diego, USA) using TruSeq SR Cluster kit v3-cBot-HS (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: ... using a Pipin prep (Sage Science, Beverly, MA, USA) and sequenced using S1 chemistry (paired-end, 2×50 bp sequencing) on a Novaseq sequencer (Illumina, Cambridge, UK). 2 biological replicates were analysed per condition.
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bioRxiv - Microbiology 2022Quote: ... 15 Gb output) was used according to the manufacturer’s instructions for library preparation and sequencing on a MiSeq 2 (Illumina, San Diego, USA). The quality of the obtained sequences was checked with FastQ files generated at the end of the run (MiSeq Reporter Software ...
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bioRxiv - Genomics 2020Quote: ... Samples were pooled and sequenced in a single lane on a MiSeq (2×250 bp paired-end, Illumina Inc., San Diego, CA) at the National Genomics Infrastructure ...
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bioRxiv - Genomics 2020Quote: ... Sequencing was performed by the Vienna Biocenter Core Facilities NGS on an Illumina HiSeq2500 platform (2×125bp; Illumina, San Diego, CA, USA).
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bioRxiv - Genomics 2021Quote: ... whole-genome of each strain was sequenced using Illumina NextSeq 2×150 bp after NextEra XT library preparation (Illumina, San Diego, CA) as in de Lastours et al ...
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bioRxiv - Genomics 2021Quote: ... and the other smaller cohort for replicating the effect size of methylation sex differences (AEMS450K2, Athero-Express Methylation Study Illumina 450K 2). RNA-sequencing was performed on plaques for which DNA methylation data was available (420 out of 485 plaques) ...
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bioRxiv - Microbiology 2020Quote: ... Paired-end sequencing libraries were constructed and sequenced as 2×151 bp using the Illumina NovaSeq platform (Illumina Inc, San Diego, CA).
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bioRxiv - Neuroscience 2021Quote: ... Immediately following the cell preparation, the pellet was resuspended in the transposase reaction mix (25 μL 2× TD buffer, 2.5 μL transposase (Illumina, San Diego, USA) and 22.5 μL nuclease-free water) ...
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bioRxiv - Plant Biology 2021Quote: ... The pooled library was sequenced on an Illumina MiSeq platform using a 2× 300-bp MiSeq Reagent Nano Kit v2 (Illumina, CA, USA).
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bioRxiv - Microbiology 2020Quote: ... and submitted for DNA sequencing using the MiSeq Reagent kit v3 with the 2 × 300 bp paired-end sequencing protocol (Illumina Inc., USA).
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bioRxiv - Cancer Biology 2020Quote: ... Sequencing was performed at The Centre for Applied Genomics (Toronto, Canada) across 2 lanes of an Illumina HiSeq2500 rapid run flowcell (Illumina, San Diego) with SR100 bp.
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bioRxiv - Neuroscience 2022Quote: ... The mRNA sequencing (read length of 2×100 bases) was performed using Illumina HiSeq2000 and 2500 machines (Illumina, San Diego, CA, USA) as per the standard Illumina RNA-seq protocol ...
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bioRxiv - Systems Biology 2022Quote: ... The barcoded amplicons were sequenced on the Illumina MiSeq platform using 2 × 250 paired-end (PE) configuration (Illumina, San Diego, CA, USA).
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bioRxiv - Immunology 2022Quote: ... Paired-end next-generation sequencing was performed on the Illumina MiSeq system 500 (2 x250 bp) (Illumina, San Diego, California, United States). TCR sequencing analysis was performed using RTCR as previously described(46).
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bioRxiv - Cancer Biology 2022Quote: ... Samples were sequenced in depth of 30 to 52 million paired-end 2×150bp reads on Illumina NovaSeq 6000 sequencer (Illumina, CA, USA).
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bioRxiv - Immunology 2021Quote: ... commercial reagents were used for unique dual indexed amplicon library generation according to the Artic protocol (NEBNext® ARTIC SARS-CoV-2 Library Prep Kit (Illumina®)) ...
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bioRxiv - Microbiology 2020Quote: ... The final pool was sequenced on Illumina MiSeq with a 2×250bp PE Illumina Reagent Kit v2 (Illumina, Inc., San Diego, CA) in the Biotechnology Center at the University of Wisconsin-Madison
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bioRxiv - Microbiology 2021Quote: ... according to the manufacturer’s instructions and sequenced using the Illumina MiSeq platform with a read length of 2 × 150 bp (Illumina, San Diego, USA). In total ...
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bioRxiv - Cancer Biology 2021Quote: Strand-specific libraries were generated using the TruSeq Stranded mRNA samples preparation kit (illumine Inc., San Diego, RS-122-2101/2) according to manufacturer’s instructions (Illumina, part #15031047 Rev.E). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... libraries were prepared with Nextera XT DNA Library Preparation Kit69 and paired-end (2×150 bp) sequencing was performed on a NextSeq 500 machine (Illumina Inc., USA) at the George Washington University Genomics Core facility ...
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bioRxiv - Plant Biology 2020Quote: RNA sequencing libraries of time course JA-treated root samples were made using 2 μg of total RNA following TruSeq® Stranded mRNA Library Preparation protocol (Illumina, USA). Equal amount libraries were pooled and sequenced using Illumina Novoseq platform ...
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bioRxiv - Genomics 2020Quote: ... 2 µl each of P7 and P5 Nextera XT Index Kit v2 index primers (Illumina Catalogue numbers FC-131-2001 to 2004) were added to each well ...
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bioRxiv - Cancer Biology 2021Quote: ... and PCR and sequencing were performed as described previously (2 x 250bp paired-end reads, on an Illumina Miseq (Lemont, IL, USA)) [68–70] ...
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bioRxiv - Cancer Biology 2021Quote: ... Strand-specific libraries were generated using the TruSeq Stranded mRNA samples preparation kit (illumine Inc., San Diego, RS-122-2101/2) according to manufacturer’s instructions (Illumina, part #15031047 Rev.E). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... The sequencing data were generated with paired-end sequencing (2 × 300 nucleotides) on the MiSeq platform (Illumina, Inc., San Diego, CA, USA).
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bioRxiv - Genomics 2020Quote: ... Up to 96 AOIs were pooled and sequenced on a NextSeq High Output v2.5 (75 cycles, 2×38 bp; Illumina, cat. no. 20024906).
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bioRxiv - Genomics 2020Quote: ... we ran the library on a 2×100 cycle sequencing run on an Illumina MiSeq v2 (Illumina, Inc., San Diego, CA, USA). The qualified library was sequenced using the Illumina NovaSeq6000 (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... and variant calling as described by the software manufacturer (https://help.geneious.com/hc/en-us/articles/360045070991-Assembly-of-SARS-CoV-2-genomes-from-tiled-amplicon-Illumina-sequencing-using-Geneious-Prime). For each sample ...
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bioRxiv - Microbiology 2022Quote: ... in a 100 µl reaction with 2 µl gDNA (∼100 ng) and primers oJMP697 and oJMP698 (nested primers with adapters for index PCR with Illumina TruSeq adapter) according to the manufacturer’s protocol using a BioRad C1000 thermalcycler with the following program ...
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bioRxiv - Microbiology 2022Quote: ... the constructed DNA libraries were sequenced with a 2 × 150 bp read length using the NextSeq 500 Sequencing System (Illumina, CA, USA).
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bioRxiv - Plant Biology 2023Quote: ... The nuclei pellet was then immediately resuspended in 25 μ L of 2x tagmentation buffer containing 2 μ L of TDE1 (Illumina). The reaction was placed at 37C for 30 minutes with gentle mixing three times ...
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bioRxiv - Evolutionary Biology 2023Quote: ... insert size 550 nt) were constructed from 1.8-2 µg gDNA with the TruSeq DNA PCR-Free kit (Illumina, San Diego, CA) and sequenced on the Illumina HiSeq 2500 by the Genomics Core Facility at Pennsylvania State University ...
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bioRxiv - Microbiology 2024Quote: ... Equal amounts of DNA from each library (except xGen-prepared) were pooled and sequenced using 2×150 bp paired-end settings on an Illumina NextSeq550 platform (Illumina, CA, USA). The xGen-prepared libraries were sequenced separately on an iSeq100 System (Illumina ...