Labshake search
Citations for Illumina :
1051 - 1100 of 1246 citations for Cytosolic arginine sensor for mTORC1 subunit 2 CASTOR2 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and finally sequenced with phiX as control using paired-end 2×250-bp chemistry on the Illumina MiSeq 2000 platform (Illumina Inc., USA)
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bioRxiv - Microbiology 2023Quote: ... The quantified and denatured libraries were sequenced using paired-end 2×250-bp chemistry with 5% PhiX as an internal control on the Illumina MiSeq 2000 platform (Illumina Inc., USA). The quality-filtered reads were de-novo assembled using SPAdes (version 3.11.1) ...
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bioRxiv - Microbiology 2023Quote: ... The prepared library was sequenced using a MiSeq sequencing system with a V3 reagent kit (300 × 2 bp; Illumina, 4–6 samples). Lake Biwa viral contigs/genomes (LBVs ...
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bioRxiv - Cell Biology 2023Quote: The libraries are sequenced in paired-end (2 x 150 cycles) through the SBS (sequencing by synthesis) technology using NovaSeq® 6000 (ILLUMINA®).
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bioRxiv - Genetics 2023Quote: ... in a 100 µl reaction with 2 µl gDNA (∼100 ng) and primers oJMP697 and oJMP698 (nested primers with partial adapters for index PCR with Illumina TruSeq adapter) according to the manufacturer’s protocol using a BioRad C1000 thermal cycler with the following program ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing was conducted on an Illumina MiSeq using a paired-end 2 x 300 base-pair method (Illumina, San Diego, CA, USA).
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bioRxiv - Systems Biology 2023Quote: ... in a 100 µl reaction with 2 µl gDNA (∼100 ng) and primers oJMP697 and oJMP698 (nested primers with adapters for index PCR with Illumina TruSeq adapter) according to the manufacturer’s protocol using a BioRad C1000 thermocycler with the following program ...
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bioRxiv - Genomics 2023Quote: ... 6.3 Gbp of data in 41.8 million reads (2 × 150 cycles) were produced using the MiniSeq System (Illumina, San Diego, Ca, USA). Illumina short reads were quality checked using FastQC v0.11 (http://www.bioinformatics.babraham.ac.uk/projects/fastqc) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... insert size 550 nt) were constructed from 1.8-2 µg gDNA with the TruSeq DNA PCR-Free kit (Illumina, San Diego, CA) and sequenced on the Illumina HiSeq 2500 by the Genomics Core Facility at Pennsylvania State University ...
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bioRxiv - Microbiology 2024Quote: ... Equal amounts of DNA from each library (except xGen-prepared) were pooled and sequenced using 2×150 bp paired-end settings on an Illumina NextSeq550 platform (Illumina, CA, USA). The xGen-prepared libraries were sequenced separately on an iSeq100 System (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing was conducted on an Illumina MiSeq using a paired-end 2 x 300 base-pair method (Illumina, San Diego, CA, USA). We estimated this should provide a mean of 60 000 reads per sample ...
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bioRxiv - Genomics 2023Quote: ... All libraries were pooled and subjected to paired-end 75 bp sequencing (paired- end 76 nt reads with the first 1 nt of Read 1 and the last 1 nt of Read 2 trimmed) using the NextSeq500 system (Illumina, CA, USA). For each library ...
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bioRxiv - Cancer Biology 2023Quote: ... Bacterial 16S rRNA gene V1-V2 region was amplified using 27F and 338R PCR primers and sequenced on a MiSeq (2×250 bp; Illumina, Hayward, CA). Bioinformatic processing and statistical analysis were performed in R software environment as described previously 62 ...
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bioRxiv - Microbiology 2022Quote: ... and variant calling as described by the software manufacturer (https://help.geneious.com/hc/en-us/articles/360045070991-Assembly-of-SARS-CoV-2-genomes-from-tiled-amplicon-Illumina-sequencing-using-Geneious-Prime). For each sample ...
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bioRxiv - Microbiology 2022Quote: ... in a 100 µl reaction with 2 µl gDNA (∼100 ng) and primers oJMP697 and oJMP698 (nested primers with adapters for index PCR with Illumina TruSeq adapter) according to the manufacturer’s protocol using a BioRad C1000 thermalcycler with the following program ...
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bioRxiv - Microbiology 2022Quote: ... the constructed DNA libraries were sequenced with a 2 × 150 bp read length using the NextSeq 500 Sequencing System (Illumina, CA, USA).
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bioRxiv - Plant Biology 2023Quote: ... The nuclei pellet was then immediately resuspended in 25 μ L of 2x tagmentation buffer containing 2 μ L of TDE1 (Illumina). The reaction was placed at 37C for 30 minutes with gentle mixing three times ...
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bioRxiv - Microbiology 2023Quote: ... The resulted pool of normalized DNA was cleaned one last time using magnetic beads at a ratio of 0.65:1 and thereafter sequenced at a commercial company (Baseclear, Leiden, The Netherlands) on the Illumina MiSeq platform using 2 × 300 bp paired-end sequencing (Illumina MiSeq PE300). Three negative control samples were included to detect possible contamination during library preparation and sequencing ...
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bioRxiv - Genomics 2023Quote: ... These five tissue-specific samples were subjected to Illumina’s TruSeq Stranded mRNA library preparation kit and sequenced on NovaSeq 6000 (Illumina, 2×150 bp). For long-read RNA sequencing ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was amplified using ARTIC v4 primer pools (https://github.com/artic-network/artic-ncov2019) and variant calling results were generated using the SIGNAL (SARS-CoV-2 Illumina GeNome Assembly Line) pipeline v1.5.0 42 ...
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bioRxiv - Cell Biology 2023Quote: ... and 2×150 bp paired-end sequencing was performed at a depth of approximately 400 million reads (HiSeq; Illumina, San Diego, USA). Cellranger (Version 3.0.2 ...
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bioRxiv - Plant Biology 2024Quote: ... A 2×150 bp paired-end sequencing library was prepared with the NovaSeq 6000 SP Reagent Kit v1.5 (Illumina, San Diego, USA) and sequenced in two lanes of a NovaSeq 6000 instrument (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... which was confirmed using the Qubit dsDNA high-sensitivity assay on a Qubit 3.0 fluorimeter and sequenced as 2 x 250 bp libraries on an Illumina Miseq (Illumina, San Diego, California) at the UConn MARS facility ...
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bioRxiv - Microbiology 2024Quote: ... The prepared DNA libraries underwent paired-end (2 x 150 bp) sequencing using the Illumina NextSeq550 platform (Illumina, San Diego, CA, USA).
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bioRxiv - Immunology 2024Quote: ... MHC class I genotyping was performed using genomic DNA isolated from PBMCs by sequencing a 150 bp region of exon 2 (Illumina MiSeq system). Sequences were analyzed by comparison to an inhouse database as previously described49 ...
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bioRxiv - Microbiology 2024Quote: ... Sequencing was performed using a 2 × 300 bp paired-end protocol on an Illumina MiSeq platform (Illumina, Inc., San Diego, CA, USA). Negative controls were included for both extraction and PCR protocols.
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bioRxiv - Microbiology 2024Quote: ... 2 µl of the plasmid was used as the template and amplified using Nextera DNA Indexes (Illumina Inc., San Diego, CA, USA) index primers ...
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bioRxiv - Genomics 2024Quote: ... Hybrid capture libraries were sequenced by Illumina short-read sequencing via NextSeq 2000 paired-end (2 x 150 bp) sequencing using standard NextSeq 2000 P3 Reagents (300-Cycles) (Illumina catalog #20040561) and analyzed using a custom hybrid capture bioinformatics pipeline called tbREVEAL developed at Tome Biosciences (described below).
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bioRxiv - Evolutionary Biology 2024Quote: ... and sequencing were performed by the University of Florida’s Interdisciplinary Center for Biotechnology Research’s Gene Expression and Genotyping core facility (ICBR; RRID:SCR_019145) using a 2 x 150 paired-end sequencing format on a NovaSeq6000 (Illumina; San Diego, CA, USA).
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bioRxiv - Molecular Biology 2021Quote: ... and 2.5 µl of each Nextera Index 1 (N7XX) and Nextera Index 2 (N5XX) primers from a Nextera DNA Sample Preparation Index Kit (Illumina, FC-121-1011). PCR was performed according to the following protocol ...
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bioRxiv - Genomics 2021Quote: ... Whole-genome sequencing data were generated using (i) an Illumina NextSeq-500 platform with a 2 × 150 nt paired-end protocol (Nextera XT library, Illumina, San Diego, CA); and (ii ...
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bioRxiv - Immunology 2021Quote: ... 62 samples including the mock community were pooled at equimolar concentrations and the library was sequenced using a MiSeq sequencer with 2 x 250 bp paired-end run (Illumina MiSeq, v2 kit). The resulting sequence data was processed using ‘Quantitative insights into microbial ecology 2’ (QIIME2 version 2020.2 ...
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bioRxiv - Plant Biology 2020Quote: ... followed by paired end sequencing (100bp x 2) on a HiSeq2000 with a HiSeq Paired-End Cluster Generation Kit and HiSeq Sequencing Kit (Illumina, San Diego, U.S.A.). Trimmomatic (ver ...
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bioRxiv - Microbiology 2020Quote: ... 30 μL of the 20 nM library was diluted to 500 uL using Illumina buffer HT1 and loaded onto a 500 cycle MiSeq (version 2) flowcell (Illumina, MS-102-2003). Paired-end run cycle parameters were 260 (Read1 ...
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bioRxiv - Plant Biology 2021Quote: ... Prepared library was loaded into Illumina platform for cluster generation and sequencing was performed using paired-end (PE) 2×150 bp library on Illunima platform (Illumina TruSeq RNA 500) (Bolger et al. ...
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bioRxiv - Plant Biology 2021Quote: ... Paired-end (2 × 150 bp) sequencing of the cDNA libraries was performed on the Illumina HiSeq 2000 (Illumina Inc., San Diego, CA, USA).
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bioRxiv - Microbiology 2021Quote: ... Goe13 and lysogens and sequenced with the MiSeq system and reagent kit V.3 (2 x 300 bp) (Illumina, San Diego, CA, USA) and the NovaSeq system (2x 150bp ...
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bioRxiv - Microbiology 2021Quote: Paired-end DNA and cDNA libraries (2 × 150 bp) were constructed using TruSeqTM DNA Sample prep kit (Illumina Inc., San Diego, CA, USA) and TruSeqTM RNA Sample prep kit (Illumina Inc. ...
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bioRxiv - Microbiology 2021Quote: ... Each pool of V4-V5 16S rRNA amplicons was sequenced (2×300nt paired end) on one lane of a MiSeq V2 sequencer (Illumina, San Diego, CA) at the Carver Biotechnology Center of the University of Illinois ...
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bioRxiv - Microbiology 2021Quote: ... All 24 samples were multiplexed together into a single High Output 2×75bp run on a NextSeq 500 instrument (Illumina, San Diego, CA). Raw reads were mapped to the C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... one RNA pool of 5 livers from each treatment and sex were independently subjected to sequencing (Illumina Novaseq 6000 paired-end (2×150)) ...
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bioRxiv - Cancer Biology 2022Quote: ... Sequencing was performed on the Illumina HiSeq 4000 paired end at 2 X126 bp or single end 126 bp using the TruSeq SBS Kit v4-HS (Illumina, Inc, California, USA).
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bioRxiv - Genomics 2022Quote: ... Libraries depleted of ribosomal RNA (figure 2 and 3A-B) were constructed with the TruSeq Total RNA with Ribo Zero plant Kit (Illumina, San Diego, USA), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... Illumina compatible P5 P7 sequences were added during post capture amplification and libraries were submitted for 2 × 150bp paired-end next generation sequencing (Illumina, San Diego, CA), targeting 15 million clusters per sample ...
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bioRxiv - Microbiology 2021Quote: Illumina libraries were prepared for paired-end 250-bp sequencing (2 × 250 bp) using the Nextera DNA Flex Library kit (Illumina, San Diego, CA). The libraries were sequenced at the UC Davis Genome Center (Davis ...
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bioRxiv - Microbiology 2020Quote: ... The pool was sequenced on an Illumina MiSeq using the 2 × 250 bp v2 kit with a 10% PhiX control following the manufacturer’s protocol (Illumina, Inc., San Diego, CA) and using custom primers developed from (55) ...
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bioRxiv - Microbiology 2021Quote: Metagenomic libraries (2×150 paired-end) were prepared and sequenced on an Illumina NextSeq 500 sequencing platform (Illumina Inc., San Diego, CA, USA). Quality of the reads and presence of adaptor sequences were checked using FastQC v.0.11.5 (Andrews ...
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bioRxiv - Genomics 2021Quote: ... ~100X sequencing coverage with Illumina short reads was generated on Illumina MiSeq (2 × 300 bp mode) instrument in a high output mode (Illumina, San Diego, CA) to support PacBio read correction and assembly polishing as needed.
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bioRxiv - Evolutionary Biology 2020Quote: ... Barcoded DNA paired end libraries with insert size of approximately 150bp were prepared using Illumina Truseq HT library prep and sequenced on 2 lanes using Illumina NovaSeq (Illumina Inc., California, USA). Raw paired-end reads were checked for quality using FastQC ...
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bioRxiv - Immunology 2021Quote: ... Sequencing was performed in the Human Immunology Core Facility at the University of Pennsylvania using an Illumina 2× 300-bp paired-end kit (Illumina MiSeq Reagent Kit v3, 600-cycle, Illumina MS-102-3003).