Labshake search
Citations for Illumina :
1001 - 1045 of 1045 citations for Mouse IgG2a Isotype Control Antibody HOPC 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Individual purified libraries were pooled into two six-sample equimolar pools containing the indexes 3-8 (NEBNext multiplex oligos for Illumina, set 1, E7335) and deep-sequenced at BGI on a HiSeq4000 (paired-end 100-bp reads).
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bioRxiv - Molecular Biology 2019Quote: ... RNA-Seq libraries were made from 1 µg total RNA per tissue sample using TruSeq stranded mRNA Library Prep Kit (Illumina, kit cat. no. 20020597) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... or 6 bp (Nextera Index Read 1 Primer/i7) additional base pairs to the 3’ ends of standard Nextera primer sequences [100] (Illumina Inc., San Diego, California). Although we have not confirmed this directly ...
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bioRxiv - Genomics 2019Quote: ... and then incubated with 5 μl of Tn5 Transposase of TTE Mix V50 in 45 μL of 1 x TTBL buffer (TruePrep® DNA Library Prep kit V2 for Illumina®, Vazyme, China) at 37°C for 30 min ...
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bioRxiv - Cell Biology 2021Quote: Libraries were generated from 1 µg of genomic DNA and high-throughput sequencing was performed on a HiSeq 3000 instrument (Illumina, San Diego, United States). Library preparation and Illumina sequencing was carried out by the Next Generation Sequencing (NGS ...
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bioRxiv - Plant Biology 2021Quote: ... 10 ng of template DNA and index primer 1 (N7xx) and index primer 2 (N5xx) of Nextera XT Index Kit v2 Set A (Illumina, San Diego, CA, USA) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... of the 16S rRNA gene and the internal transcribed spacer (ITS) 1 rDNA variable region on the Illumina MiSeq platform (Illumina, San Diego, CA, USA), as detailed previously (Phoma et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA (1 μg) was then converted into cDNA libraries according to the Illumina TruSeq Stranded mRNA Sample Preparation Guide (Illumina, San Diego, CA, USA) following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... Pooled libraries were diluted to 1.8 pM for 1×86 bp single-end sequencing with 75-cycle High Output v2 Kit on the NextSeq 550 Sequencing System (Illumina, San Diego, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: ... Pooled RPFs were diluted to 100 ng/uL and 1 ug was used as input for rRNA depletion using RiboZero Gold (part of Illumina 20020598, San Diego, CA) per manufacturer protocol ...
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bioRxiv - Physiology 2020Quote: ... RNA-seq libraries were sequenced using paired-end reads (50-nt read 1 and 25-nt read 2) on a NextSeq 500 instrument (Illumina, San Diego, CA, USA). Obtained reads were mapped to the mouse GRCm38.p6 genome using STAR (version 2.7.3a) ...
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bioRxiv - Genomics 2021Quote: ... reporter cDNA was PCR amplified using a reporter specific forward primer and a reverse primer binding the anchor sequence of the oligo- dT primer (corresponding to the Illumina TruSeq Read 1 sequence):
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bioRxiv - Neuroscience 2021Quote: ... Libraries were pooled and loaded for clustering on 1 lane of a Single-read Illumina Flow cell (for an average of 50 million of reads per library) (Illumina, San Diego, CA, USA). Reads of 100 bases were generated using the TruSeq SBS chemistry on an Illumina HiSeq 4000 sequencer (Illumina ...
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bioRxiv - Cancer Biology 2022Quote: ... Libraries were pooled and sequenced single-end (1×75) on an Illumina NextSeq 500 using the High output V2 kit (Illumina Inc., San Diego CA) at a sequencing depth of 23-31 million reads ...
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bioRxiv - Genomics 2022Quote: ... The concentration of amplified cDNA was measured with Qubit and 1 ng was used as input for Nextera XT (Illumina, San Diego, CA, USA) library preparation using New P5 SMART PCR oligo and Nextera index oligos ...
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bioRxiv - Genomics 2022Quote: ... 2 μg of Rohu-1 genomic DNA was used with an Illumina TruSeq DNA PCR-free Library Prep Kit (Illumina, San Diego, CA, USA) to create an Illumina sequencing library ...
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were prepared from purified RNA (∼1 μg) using Illumina TruSeq standard mRNA library preparation kit as per manufacturer protocol (Illumina Inc., San Diego, CA). Briefly ...
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bioRxiv - Plant Biology 2023Quote: ... 1 μg total RNA was used for preparation of cDNA libraries with the TruSeq Small RNA Library Preparation Kit (Illumina, San Diego, CA, USA). Libraries were sequenced as 50 bp paired-end reads on an Illumina Genome Analyzer ...
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bioRxiv - Cancer Biology 2022Quote: ... Libraries were pooled and sequenced single-end (1×75) on an Illumina NextSeq 500 using the High output V2 kit (Illumina Inc., San Diego CA). The RNA-seq samples were sequenced at a sequencing depth of 11-16 million reads.
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bioRxiv - Cell Biology 2024Quote: ... 8 bases for index 1 and 8 bases for index 2) on NextSeq 500 using the NextSeq 500 High Output Kit 75-cycles (Illumina, Cat# FC-404-1005). Flow lanes were loaded at 1.8pM ...
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bioRxiv - Cell Biology 2024Quote: ... 8 bases for index 1 and 8 bases for index 2) on NextSeq 500 using the NextSeq 500 High Output Kit 75-cycles (Illumina, Cat# FC-404-1005). Flow lanes were loaded at 1.8pM ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA samples were then diluted to 0.2-0.3 ng per uL and 1 ng of cDNA was carried over into library preparation using the Nextera XT DNA Library Preparation Kit (Illumina, cat. # FC-131-1096) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Libraries were prepared from 1 µg of input DNA using a TruSeq DNA Sample Prep Kit according to the manufacturer’s instructions (Illumina, Inc., San Diego, CA, USA). The sheared DNA fragments underwent end-repair ...
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bioRxiv - Microbiology 2023Quote: ... a sequencing library was generated from 1 ng of the resulting SAG DNA with the NexteraXT library preparation kit (Illumina, San Diego, CA, USA) and the library was then sequenced on an Illumina MiSeq instrument (2×300 bp) ...
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bioRxiv - Bioengineering 2023Quote: ... Sequencing was performed in paired-end mode with HighOutput flow cell (read 1: 28 cycles and read 2: 90 cycles) and a NextSeq 550 sequencer (Illumina, San Diego, CA, USA) at the Transcriptome Core Facility of the Institute in Regenerative Medicine and Biotherapy ...
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bioRxiv - Plant Biology 2024Quote: ... RNA-seq libraries were prepared from 1 μg of total RNA using the Illumina TruSeq RNA Library Prep Kit (Illumina, San Diego, CA, USA) and sequenced on an Illumina NovaSeq 6000 platform (2 × 150 bp ...
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bioRxiv - Genetics 2024Quote: ... which uses proximity extension assay technology coupled to a readout methodology based on next generation sequencing (Illumina NovaSeq 6000, NextSeq 550, and NextSeq 2000; all manufactured by Illumina; appendix 1 p 2), to quantify protein targets ...
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bioRxiv - Genetics 2021Quote: ... A second PCR step was run to add the index primers for sequencing (NEBNext Multiplex Oligos for Illumina Dual Index Primers Set 1 and 2). PCR products were purified using SPRIselect beads (Beckman ...
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bioRxiv - Cancer Biology 2020Quote: ... sequencing was run in the rapid run flow cell and paired-end sequenced (read 1 – 26 bp, read 2 – 100 bp) on a HiSeq 1500 (Illumina, San Diego, CA 92122 USA).
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bioRxiv - Plant Biology 2021Quote: ... for 96-plex Genotyping-by-Sequencing (GBS) library preparation using the ApeKI restriction enzyme and sequenced (1 × 100 bp) on the Illumina HiSeq 2500 (Illumina, Inc., San Diego, CA, USA) platform.
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bioRxiv - Microbiology 2019Quote: ... Five paired-end libraries were prepared using 1 ng of extracted DNA and MiSeq Technology with the paired-end method and the Nextera XT kit (Illumina Inc., San Diego, CA, USA). DNA was fragmented ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The libraries were sequenced after on-board cluster generation for 80 cycles using 1× HiSeq Rapid SBS Kit v2 (Illumina, Cat. No. FC-402-4022) and HiSeq SR Rapid Cluster Kit v2 (Illumina ...
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bioRxiv - Cell Biology 2022Quote: ... a PCR enrichment step was performed (8 cycles) using unique primer pairs for each library (NEBNext Multiplex Oligos for Illumina-Dual Index Primers Set 1).
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bioRxiv - Genomics 2022Quote: Genomic libraries were prepared from 1 ng of high-quality genomic DNA using the Nextera DNA Flex Library Prep kit (now called Illumina DNA Prep, Illumina C.A., USA), following the manufacturer instructions ...
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bioRxiv - Cell Biology 2022Quote: The libraries were generated using depleted RNA obtained from 1 μg of total RNA by TruSeq Sample Preparation RNA Kit (Illumina, Inc., San Diego, CA, USA) according to the manufacturer’s protocol without further modifications ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA quality was determined and 1 ug RNA was used in library preparation using TruSeq RNA library preparation kit (Illumina, cat no RS-122-2001). Library preparation was performed according manufacturers protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... we dispensed 600 nL of tagmentation reaction mix containing Tagmentation DNA buffer (TD) and Amplicon Tagment Mix (ATM) at 2:1 v/v ratio (Nextera kit, Illumina, cat. no. FC-131-1096) into each well and performed tagmentation at 55° C for 5 min followed by hold at 4° C in a PCR thermocycler ...
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bioRxiv - Genomics 2023Quote: The libraries were sequenced on NextSeq 500 machine with customized sequencing primers as follows: Read 1 sequencing primer: ACA CTC TTT CCC TAC ACG ACG CTC TTC CGA TCT (the same as Illumina Tru-seq i5 sequencing primer), Read 2 sequencing primer ...
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bioRxiv - Cell Biology 2023Quote: cDNA libraries were prepared from 1 μg total RNA of each sample using the TruSeq Stranded mRNA Sample Prep Kit (Illumina, Inc., San Diego, CA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... and 1 μl of each Nextera XT Index Kit v2 set B or set C barcoding primer (Illumina, Cat# FC-131-2002 or FC-131-2003). These reactions were then incubated at 95°C for 15 minutes ...
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bioRxiv - Microbiology 2019Quote: ... RNA from cultures grown on PM-4-HBA and PM-syringic acid was processed and sequenced at the University of Wisconsin-Madison Biotechnology Center (Illumina HiSeq2500, 1×100 bp, single end). RNA from cultures grown on PM-succinate was processed and sequenced at the U.S ...
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bioRxiv - Molecular Biology 2020Quote: ... the NEBNext Ultra II DNA Library Preparation kit was used to generate libraries using 5-10 ng of input or immunoprecipitated DNA and barcode adaptors (NEBNext Multiplex Oligos for Illumina (Set 1, E7335 and Set 2, E7500)) ...
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bioRxiv - Genomics 2021Quote: ... 1 μg of DNA-free total RNA was subjected to sRNA library construction according to manufacturer’s protocol (Illumina, cat. No. RS-200-0012 and RS-200-0024).
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bioRxiv - Microbiology 2021Quote: ... Nextera XT indices were used to generate libraries from 1 ng of cDNA material for each sample following the instructions provided by Illumina (Illumina FC-131-1096 and FC-131-2001). Library preparations were purified using AMPure XP beads and checked for quality using the Agilent Bioanalyzer at Stanford Functional Genomics Facility (SFGF) ...
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bioRxiv - Genomics 2022Quote: ... the ScriptSeq™ Index PCR Primers (Sets 1 to 4) and the FailSafe™ PCR enzyme system (all sourced from Epicentre®/Illumina® Inc., Madison, WI, USA) according to the manufacturer’s instructions ...