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Citations for Illumina :
51 - 93 of 93 citations for Pemetrexed disodium Impurity 30 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Axon-secreted chemokine-like Orion is a signal for astrocyte infiltration during neuronal remodelingbioRxiv - Neuroscience 2020Quote: ... Genomic DNA was extracted from 30 adult females (mutant and control) and directly sequenced on a HiSeq2000 next-generation sequencing platform (Illumina). Bioinformatics analysis for read alignment and variant investigation was carried out through the 72 kb selected by duplication mapping (see above ...
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bioRxiv - Neuroscience 2020Quote: ... we used one batch of 20 ng of total photoreceptor (n = 8) RNA and 30 ng for the other three batches (n = 24) according to the manufacturer’s protocol (Illumina Platforms). For generating libraries from RPE samples we used 20 ng of RNA ...
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bioRxiv - Molecular Biology 2020Quote: ... nuclei were isolated from 100,000 cells and sequencing adapters were transposed for 30 minutes at 37°C using 5 μl of TDE1 (Nextera Tn5 transposase, Illumina). After PCR and gel purification ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Approximately 30% of Illumina libraries were constructed in-house with the Nextera DNA Library Preparation Kit (Illumina, FC-121-1030) using a protocol based off of previously published protocols51,52 ...
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bioRxiv - Immunology 2020Quote: ... The samples were mixed in equimolar ratios to obtain 30 μl of a final concentration of 10-nM and sequenced by Illumina MiSeq (v3 kit ...
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bioRxiv - Evolutionary Biology 2022Quote: ... using a v3 Miseq sequencing kit (300 × 2, MS-102-3003) with 30% PhiX (Illumina, Inc. San Diego, CA, USA) and a starting concentration of 4nM denatured to a final concentration of 8pM.
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bioRxiv - Cancer Biology 2024Quote: ... the sequencing was performed under the setting of single read 1×51 bp to generate ∼30 million reads per sample on HiSeq 1000 sequencer (Illumina).
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bioRxiv - Genomics 2024Quote: ... before carrying out the Tn5 transposase reaction with Illumina Tagment DNA TDE1 enzyme (37°C, 30 min, 1000 RPM, Illumina) following the standard “OmniATAC protocol” (Corces et al. ...
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bioRxiv - Genomics 2024Quote: ... Barcoded libraries were sequenced to a read depth of 30 million 150 base pair paired-end reads using the Novaseq 6000 (Illumina).
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bioRxiv - Microbiology 2023Quote: ... DNA-Seq libraries were prepared using Nextera XT library preparation kit with 700 pg DNA input per sample and 6:30 min tagmentation at 55 °C and barcoded using Nextera XT indexes (Illumina). RNA-Seq libraries were prepared using KAPA RNA HyperPrep kit (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... The size selected fragments were circularization by blunt end ligation for 16 hrs at 30°C using circularization ligase (Illumina). Non-circularized fragments were eliminated by DNA exonuclease treatment ...
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bioRxiv - Systems Biology 2023Quote: ... Purified amplicons were then pooled (with 30% customized random library to increase the diversity) and sequencing was performed by NextSeq 500 sequencing platform (Illumina) with custom primers (Integrated DNA Technologies):
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bioRxiv - Genomics 2023Quote: ... coverage and eight additional female genomes (4 Asian and 4 African) at ~30× (1 lane of Illumina Hiseq X Ten) coverage using 10x Linked-Reads at HudsonAlpha Institute of Biotechnology ...
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bioRxiv - Biochemistry 2023Quote: ... using 100 bp paired-end reads at a depth of ∼30 million reads/sample and concurrently run with the PhiX quality control library (Illumina).
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bioRxiv - Cell Biology 2022Quote: Sequencing libraries were prepared by polyA selection for mRNA and sequenced to a depth of 20-30 Mio reads (HiSeq 4000 2 × 150 paired-end configuration, Illumina). Sequence reads were trimmed to remove possible adapter sequences and nucleotides with poor quality using Trimmomatic v.0.36 ...
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bioRxiv - Microbiology 2023Quote: ... 200 ng of PCR product for each sample were normalized in 30 µl volume for NGS library construction using Illumina DNA Prep (M) Tagmentation kit (Illumina). IDT for Illumina Nextera DNA Unique Dual Indexes were used for pooling multiple samples together ...
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bioRxiv - Genetics 2023Quote: ... 30 million paired-end 150bp reads were obtained for each ChIP-ISO and input sample using a NextSeq 2000 (Illumina) instrument ...
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bioRxiv - Cancer Biology 2024Quote: ... Library preparation was performed by the YCGA followed by sequencing to a depth of 30 million paired-end 150 bp reads (2×150) per sample on NovaSeq (Illumina).
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bioRxiv - Genetics 2024Quote: ... Individual sequencing barcodes were added to each sample by amplifying the entire 40 μL elution in a 100 μL Q5 NEBNext reaction with 0.5 μM of TruSeq_Universal_Adapter primer and a reverse primer containing a unique 8 bp index (Illumina_Multiplex, Supplementary Table 30) for sample demultiplexing post-sequencing ...
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bioRxiv - Cell Biology 2020Quote: ... Tagmentation was performed during 30 minutes at 25°C in a 50 μl reaction in TD Buffer (Illumina, FC-121-1030) containing 2.5 μl Tn5 Transposes (Illumina ...
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bioRxiv - Developmental Biology 2022Quote: ... After centrifugation supernatant was used for RNA cytosolic fraction by adding to 1 ml of TRIZOL and isolated nuclei of CMs were incubated in 30 µl and of EC in 10 µl transposase mixture with 5% Nextera Tagment DNA Enzyme TDE (Illumina #15027916) in transposition buffer (20 mM Tris-HCl pH 7.6 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The Omni-C library was sequenced to approximately 30× coverage on an Illumina NovaSeq 6000 instrument (Illumina, Inc., San Diego, CA).
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bioRxiv - Cell Biology 2024Quote: ... A total of 25-30 million reads per sample were obtained using paired-end 100 pb read length (Illumina NovaSeq 6000). A quality control analysis of the RNA-seq raw data was performed with FastQC v0.12.0 (Babraham Bioinformatics ...
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bioRxiv - Genetics 2023Quote: ... Enriched DNA fragments were then cleaned up using AMPure XP beads and final DNA libraries eluted in 30 uL of RSB (Illumina #20020599).
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bioRxiv - Genomics 2023Quote: ... Urine samples were then sequenced with (either the 2 x 150 bp or 2 x 75 bp configuration) to a mean depth of 30 M reads per sample on NextSeq 2000 (Illumina Inc); sequencing length did not impact gene detection efficiency (Supplementary Fig ...
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bioRxiv - Genomics 2023Quote: ... The genome of TY-11 was sequenced using a whole-genome (WGS) strategy (30-fold genome coverage) with NextSeq 550 (Illumina, USA).
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bioRxiv - Genomics 2024Quote: ... The normalized libraries were sequenced as paired end 100bp reactions targeting 30 million reads/sample on the Illumina NovaSeq 6000 (Illumina, CA). The raw FASTQ files were processed through an in-house bioinformatics pipeline including adapter trimming by TrimGalore (v0.6.10 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Reads were trimmed to remove adapter sequences and low-quality regions (Q < 30 and Q < 20 for Illumina and 454 data, respectively) were removed using Cutadapt 1.4.1 (Martin 2011 ...
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bioRxiv - Cell Biology 2021Quote: ... 20 ul H2O) at 37◻°C for 30◻min (TruePrep® DNA Library Prep Kit V2 for Illumina, Vazyme, China). After the tagmentation ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were sequenced in depth of 30 to 52 million paired-end 2×150bp reads on Illumina NovaSeq 6000 sequencer (Illumina, CA, USA).
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bioRxiv - Genomics 2022Quote: ... fecal microbial DNA of the two subjects was sequenced by paired-end sequencing with a coverage of more than 30 million reads per sample using Illumina HiSeq 2500 (Illumina, CA, USA) and BGISEQ-500 (BGI ...
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bioRxiv - Bioengineering 2024Quote: ... The resulting cell nuclei were subjected to incubation for 30 min with Tagment DNA TDE1 Enzyme and Buffer (Illumina, #FC-121-1030). This step fragmented the chromatin and inserted sequencing adapters ...
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bioRxiv - Genetics 2020Quote: ... one unit of input was diluted to 30 μl and mixed with 50 μl of 2x TD buffer and 20 μl of TDE (Illumina 20034197, Lot 20436911), and incubated at 55 °C for 10 min (Note ...
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bioRxiv - Cell Biology 2022Quote: ... the sequencing was performed under the setting of single read 1×51 bp to generate ∼30 million reads per sample on HiSeq 1000 sequencer (Illumina, San Diego, CA). Data analysis was performed as described before (56).
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bioRxiv - Cancer Biology 2020Quote: ... is a 30-gene NGS test for somatic mutations in myeloid neoplasms using the Truseq Amplicon Cancer Panel kit (Illumina, San Diego, CA). It contains amplicons for hot-spot locations of the following genes ...
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bioRxiv - Immunology 2021Quote: ... 300 cycles) or asymmetric (r1:30 cycles and r2: 270 cycles) fashion using the MiSeq PE-300 v3 reagent kit (Illumina, MS-102-3001) or NovaSeq 6000 S1 reagent kit (Illumina ...
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bioRxiv - Genomics 2023Quote: ... Isolated nuclei were lysed and transposed for 30 minutes at 37°C using the prokaryotic Tn5 transposase system (Nextera DNA library kit, Illumina, FC-121–1030). Transposed DNA was then purified on Diapure columns (Diagenode Cat# C03040001) ...
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bioRxiv - Microbiology 2021Quote: ... DNA yields of all samples were diluted with EB buffer to 30 ng/μl concentrations and DNA samples were sent to MicrobesNG for sequencing (Illumina 30x coverage; http://www.microbesng.uk). MicrobesNG conducted library preparation using Nextera XT Library Prep Kit (Illumina ...
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bioRxiv - Genetics 2023Quote: ... Individual libraries were pooled 30-plex at equimolar concentrations and sequenced on one lane of an Illumina HiSeq 2500 (Illumina, Inc, San Diego, CA) employing a paired-end ...
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bioRxiv - Microbiology 2023Quote: ... 7pM of the pooled library with 30% PhiX phage as a control was sequenced using a 300-cycle Illumina MiSeq Kit (Illumina, Inc., San Diego, CA), generating 150bp paired-end reads ...
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bioRxiv - Evolutionary Biology 2023Quote: ... by processing the short-read sequencing data of the HG001 genome (SRR8454588, data generated by Illumina HiSeq 4000 with a coverage of 30 fold), and benchmarking using BEDtools tool.
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bioRxiv - Genomics 2020Quote: ... Further sample QC was performed by Illumina to ensure that the concentration of the DNA was > 30 ng/μl and that every sample generated high quality genotyping results (Illumina Infinium Human Core Exome microarray). Samples with a repeated array genotyping call rate < 0.99 ...
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bioRxiv - Microbiology 2024Quote: ... and controls (n=30) were amplified using PCR with the F515 and R806 primer pair and sequenced using Illumina MiSeq platform (Illumina Inc., San Diego, CA, USA) (82) ...