Labshake search
Citations for Illumina :
9551 - 9600 of 10000+ citations for Cow Putative Phospholipase B Like 2 PLBD2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... 1ug of total RNA was then used to generate sequencing libraries using the TruSeq RNA Sample prep kit v2 (Illumina RS-122-2001), as per recommended instructions ...
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bioRxiv - Microbiology 2021Quote: ... Next the amplicons were checked for their size and purity on a 1.5 % agarose gel and if suitable subjected to the index PCR using the Nextera XT Index Kit v2 Set C/D (Illumina, FC-131-2003). After index PCR the samples were cleaned again with AmPure XP Beads (Beckman Coulter Life Science Cat ...
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bioRxiv - Microbiology 2020Quote: ... The genome was sequenced with Illumina technology using a GAIIx sequencing platform on paired-end libraries prepared with the Nextera XT DNA Kit (Illumina, San Diego, CA). A total of 84 contigs (0.5 to 330 kb ...
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bioRxiv - Plant Biology 2021Quote: RNA-seq libraries were constructed by the POPS platform (IPS2) using the TruSeq no stranded mRNA library prep kit (Illumina®, California, U.S.A.) according to the supplier’s instructions ...
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bioRxiv - Neuroscience 2021Quote: Library preparation and sequencing of the samples were conducted at the Mayo Clinic Genome Analysis Core using TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA). The library was sequenced on Illumina HiSeq2000 instruments ...
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bioRxiv - Neuroscience 2021Quote: ... 300 pg of cDNA from each sample was used as input for library preparation with the Nextera XT DNA Library Prep Kit (Illumina FC-131-1096). Fragmentation and adaptors insertion were performed by tagmentation ...
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bioRxiv - Genomics 2021Quote: ... Genomic DNA libraries for short- and long-read sequencing were generated with the TruSeq DNA PCR-Free Kit (Illumina, San Diego, CA, USA) and the SMRTbell Express Template Prep Kit 2.0 (PacBio ...
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bioRxiv - Immunology 2020Quote: ... 500 ng of total RNA underwent polyA selection and TruSeq library preparation according to instructions provided by Illumina (TruSeq Stranded mRNA LT Kit), with 8 cycles of PCR ...
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bioRxiv - Microbiology 2020Quote: ... Individual libraries were constructed from some of the strain DNA preparations described above using an Illumina Nextera XT DNA sample preparation kits with appropriate indices tags according to the manufacturer’s instructions (Illumina Inc., San Diego, CA). The libraries were pooled together and run on an Illumina MiSeq DNA sequencer (Illumina Inc. ...
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bioRxiv - Microbiology 2021Quote: The cDNA synthesis and library construction were done using a TruSeq Stranded mRNA LT Sample Prep Kit (Illumina Inc., San Diego, CA, USA) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: Messenger RNA capture based libraries were prepared starting from 8.5 µL DNase treated and spike-in supplemented RNA eluate using the TruSeq RNA Exome Library Prep Kit (Illumina, San Diego, CA, USA). Each sample underwent individual enrichment according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... is a 30-gene NGS test for somatic mutations in myeloid neoplasms using the Truseq Amplicon Cancer Panel kit (Illumina, San Diego, CA). It contains amplicons for hot-spot locations of the following genes ...
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bioRxiv - Cancer Biology 2020Quote: ... Libraries were pooled and single end-sequenced (1×75) on the Illumina NextSeq 500 using the High output V2 kit (Illumina, San Diego, CA). Quality control was performed using Fastqc (v0.11.5 ...
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bioRxiv - Neuroscience 2020Quote: ... Library preparation for RNA-Seq was performed using the TruSeq™ RNA Sample Prep Kit v2 (Illumina, Cat. N°RS-122-2002, U.S.A.) starting from 50 ng of total RNA ...
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bioRxiv - Developmental Biology 2021Quote: ... libraries were prepared using the Quant-seq 3’ mRNA Library Prep Kit FWD (Lexogen, Greenland, NH) and single-end 75bp reads were sequenced on the NextSeq 500 (Illumina, San Diego, CA). Genes queried for hiPSC identity were listed on the TaqMan hPSC Scorecard Assay (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Next generation sequencing and bioinformatics analysis was performed by the National Genomics Infrastructure (NGI) at the Science for Life Laboratory on the HiSeq 2500 System using the HiSeq Rapid SBS Kit v2 (Illumina, San Diego, CA) generating >13.5 M reads/sample ...
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bioRxiv - Genetics 2022Quote: ... Libraries were constructed using the Lexogen QuantSeq 3′ mRNA-Seq Library Kit FWD (Lexogen, Greenland, NH) and sequenced on an Illumina NextSeq 500 producing 85 nt single-end reads (Illumina, San Diego, CA) with each plate being split in half and each half being sequenced on a single lane to achieve desirable coverage.
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bioRxiv - Immunology 2022Quote: ... The amplicons were quantified using KAPA Library Quantitation kit (KAPA, Cape Town, South Africa) and an equimolar amount of each sample was sequenced on the MiSeq System (Illumina, San Diego, CA) with 2×150 paired-end run parameters ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA-Seq libraries were generated from 300 ng of total RNA using TruSeq Stranded mRNA Library Prep Kit and IDT for Illumina - TruSeq RNA UD Indexes (96 Indexes, 96 Samples) (Illumina, San Diego, USA), according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Post-ligation cleanup proceeded according to Illumina’s instructions with 110 µL Sample Purification Mix from the TruSeq Methyl Capture EPIC LT Library Prep Kit (Illumina catalog # FC-151-1002). After purification ...
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bioRxiv - Genetics 2022Quote: ... Individual barcoded libraries were randomly multiplexed into groups of four samples using equimolar quantities and sequenced on a MiSeq using a v3 600 reagent kit (Illumina, San Diego, CA) to generate paired-end 250 bp reads ...
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bioRxiv - Genetics 2022Quote: ... Output total RNA was depleted for ribosomal RNA and prepped for RNA-seq using the Scriptseq Complete Gold (Epidemiology) kit (Illumina, San Diego, CA). A total of ~50 ng of ribosomal depleted RNA was used as an input to transcriptome libraries ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Two independent paired-end libraries were constructed per population from these DNA pools using the Genomic DNA Sample Preparation Kit (Illumina, San Diego, CA) (technical replicates) ...
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bioRxiv - Microbiology 2019Quote: ... we sequenced the pooled library on the Illumina MiSeq platform using 250 × 250 base pair (bp) paired-end reads (Illumina Reagent Kit v2) at the Indiana University Center for Genomics and Bioinformatics Sequencing Facility ...
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bioRxiv - Cancer Biology 2020Quote: RNA libraries were prepared from 550ng of total RNA using the TruSeq Stranded Total RNA HT kit with Ribo-Zero Gold (Illumina, San Diego, USA) as per manufacturer’s instructions and subjected to paired end 75 sequencing on a HiSEQ4000 platform (Illumina ...
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bioRxiv - Genomics 2019Quote: ... and a 3 kb mate-pair library using an Illumina Nextera Mate Pair Sample Preparation kit and following the manufacturer’s protocol (Illumina, San Diego, CA, USA). Both libraries were sequenced on an Illumina HiSeq 2500 at the Bauer Core ...
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bioRxiv - Genomics 2019Quote: ... An aliquot of each DNA sample was diluted to 0.2 ng/μl and used as input DNA to the Nextera XT DNA Library Preparation Kit (Illumina Inc. San Diego, CA), according to manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA-Seq libraries preparation and sequencing was performed by the IGA Technology Services (Udine, Italy) using the Illumina TruSeq Stranded mRNA Kit (Illumina, San Diego, CA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Subsequent amplicon sequencing was performed on a MiSeq DNA-sequencing platform using a 600-cycle MiSeq Reagent Kit v3 (Illumina, San Diego, CA). Sequencing yielded 5,594,739 paired end sequences ...
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bioRxiv - Immunology 2019Quote: ... Non-stranded sequencing libraries were prepared using Illumina TruSeq RNA library kits and sequenced on an Illumina Hi Seq2500 using a SE70 protocol (Illumina, San Diego, CA). Raw sequenced reads were trimmed for adapter and bar-codes sequences and quality assessed using FastQC (http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) ...
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bioRxiv - Genomics 2019Quote: ... The barcode sequencing libraries were subjected to a final bead clean-up SPRIselect reagent and quantified by quantitative PCR (KAPA Biosystems Library Quantification Kit for Illumina platforms; KK4824). Sequencing libraries were loaded on an Illumina sequencer with 2 × 50 paired-end kits using the following read length ...
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bioRxiv - Developmental Biology 2019Quote: ... and Nextera XT DNA sample prep and indexing library preparation kits as recommended by the manufacturer (FC-131-2001, FC-131-2004, and FC-131-1096, Illumina, San Diego, CA). Resulting short fragment libraries were checked for quality and quantity using a Bioanalyzer (Agilent ...
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bioRxiv - Synthetic Biology 2019Quote: ... RNAseq libraries were produced from all samples according to the manufacture protocol (NEBNext UltraII Directional RNA Library Prep Kit for Illumina, cat no. E7760), using 800 ng total RNA per sample ...
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bioRxiv - Immunology 2019Quote: ... Sequencing was performed on the Illumina HiSeq 4000 single end 125 bp using the TruSeq SBS Kit v4-HS (Illumina, Inc, California, USA).
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bioRxiv - Immunology 2019Quote: ... Sequencing was performed on the Illumina HiSeq with single end 125 bps using the HiSeq 3000/4000 SBS Kit (Illumina, Inc, California, USA). Reads quality was checked with FastQC (https://www.bioinformatics.babraham.ac.uk/projects/fastqc/ ...
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bioRxiv - Systems Biology 2019Quote: ... Sequencing library DNA preparation was performed using the Tn5 tagmentation-based method with 1/4 volumes of the Nextera XT DNA Library Preparation Kit (Cat# FC-131-1096, −2001, −2002, −2003, and −2004, Illumina, San Diego, CA) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2019Quote: ... 1.5 pM of pool (combined with 40% spiked-in) was loaded onto a NextSeq 500 Mid-Output Kit (150 cycles) cartridge (catalog #FC-102-1001; Illumina, San Diego, CA) for high throughput sequencing on a NextSeq 500 instrument (Illumina) ...
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bioRxiv - Systems Biology 2020Quote: ... Total-RNA stranded transcriptome libraries were prepared using the TruSeq Stranded Total RNA LT Sample Prep Kit starting from the fragmentation step in the manufacturers protocol (Illumina, RS-122-2201). Paired-end sequencing (2×125-bp ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RNA-seq libraries were generated using SENSE Total RNA-seq Library Prep Kit v2 (Lexogen, Vienna, Austria) following the manufacturer’s protocol and sequenced on NextSeq 500 (Illumina, San Diego, CA, USA) using single-end 75 bp chemistry.
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bioRxiv - Developmental Biology 2020Quote: ... cDNA libraries were then tagmented using the Illumina/Nextera DNA prep kit (FC 121-1030) with index tags from Illumina (FC 121-1031), cleaned up with Ampure XP beads ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA-seq libraries were prepared from a minimum of 10ng of total RNA using the Illumina Truseq mRNA stranded library prep kit (Illumina, San Diego, USA) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2019Quote: ... The cleaved RNA fragments were reverse-transcribed to create the final cDNA library for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), with an average of 300 bp (±50 bp ...
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bioRxiv - Genomics 2020Quote: ... Sequencing was performed on the Illumina HiSeq 2500 single end 126 bp using the TruSeq SBS Kit v4-HS (Illumina, Inc, California, USA).
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bioRxiv - Cell Biology 2019Quote: ... The resulting full-length enriched cDNA library was processed into the sequencing library using the Nextera XT DNA Library Preparation Kit (Illumina, FC-131-1096). The quality of the libraries was inspected by size and concentration by agarose gel electrophoresis before pooling the libraries ...
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bioRxiv - Molecular Biology 2019Quote: ... Sequencing was performed on the Illumina HiSeq 2500 single end 126 bp using the TruSeq SBS Kit v4-HS (Illumina, Inc, California, USA).
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bioRxiv - Neuroscience 2020Quote: ... cDNA libraries with an average size of 1.5-2 kb were tagmented and indexed during a second PCR amplification step with the Illumina Nextera XT DNA preparation kit (Illumina, FC-131-1024). Tagmentation was performed according to the manufacturer’s protocol with an input of 500 pg cDNA and amplicon tagment mix for 5 min at 55 °C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... All samples and all libraries were of good quality and 75 bp single end reads were generated for each library using the Illumina NextSeq®500 v2.5 High-output 150 cycle kit (Illumina Inc., Cambridge, UK). A total of 153,887,020 reads were generated for the 36 samples (4,274,639 ± 1,905,806 reads per sample).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and 75 bp paired-end reads were generated for each library using the Illumina NextSeq®500 v2.5 High-output 150 cycle kit (75bp paired-end reads, Illumina Inc., Cambridge, UK). A total of 455,308,427 reads (average of 12,647,456 ± 2,461,518 reads per sample ...
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bioRxiv - Microbiology 2021Quote: ... The sequencing amplicon pools were diluted to 0.2 ng/µl and tagmented with Nextera XT library prep kit (Illumina, Cat#FC-131-1024). Nextera libraries were dual-barcoded and sequenced on an Illumina NextSeq1000 instrument ...
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bioRxiv - Physiology 2020Quote: ... Library preparation was performed with 1μg total RNA using the TruSeq Stranded mRNA Library Prep Kit High Throughput (Cat# RS-122-2103, Illumina, San Diego, CA, USA). Libraries were quality-checked on the Fragment Analyzer (Advanced Analytical ...