Labshake search
Citations for Illumina :
901 - 950 of 1053 citations for 6 1 BROMO ETHYL 4 CHLORO 5 FLUOROPYRIMIDINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... and sequenced as 100 base single end reads on an Illumina HiSeq 2500 using TruSeq Rapid SBS sequencing kit version 1 and HCS version 2.0.12.0 data collection software (Illumina, San Diego, CA). Base-calling is performed using Illumina’s RTA version 1.17.21.3 ...
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bioRxiv - Microbiology 2023Quote: ... Amplicons were quantified with Qubit and sequenced as 1 x 78 nt reads on MiSeq or NextSeq 1000 instruments (Illumina)
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bioRxiv - Microbiology 2023Quote: ... Libraries for next-generation sequencing were constructed from 1-ng amplified genomes using the Nextera XT DNA Library Preparation Kit (Illumina) and sequenced using the MiSeq Reagent Kit v3 on the MiSeq Platform (Illumina) ...
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bioRxiv - Genomics 2023Quote: ... 96 libraries from 16 animals in batch 1 were performed using TruSeq RNA Library Preparation Kit v2 (RS-122-2001, RS-122-2002, Illumina) and were sequenced 50 base pairs ...
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bioRxiv - Genomics 2023Quote: ... Libraries for RNA-seq were prepared from 1 μg of total RNA using Truseq Total RNA Library Prep Kit (Illumina) following manufacturer’s protocols ...
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bioRxiv - Developmental Biology 2023Quote: ... Nuclei were mixed with 50 µl of transposition reaction mix containing Tagment DNA Enzyme 1 (Nextera DNA Library Preparation Kit, Illumina). The transposition reaction was carried out at 37°C and 600rpm for 30 min ...
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bioRxiv - Developmental Biology 2023Quote: Ribosomal RNAs were depleted from 1 µg of total RNA using TruSeq Stranded Total RNA kit Ribo-Zero components (Illumina) according to the manufacturer’ s protocol ...
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bioRxiv - Physiology 2023Quote: ... 1 ng of amplified cDNA was used to generate barcoded libraries using the Nextera XT DNA library preparation kit (Illumina). Basically ...
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bioRxiv - Genomics 2023Quote: ... All cDNA libraries were sequenced on an Illumina NextSeq 500 instrument with 1.45 pM input and 1% PhiX control library spiked in (Illumina, USA) targeting 30-40 Million reads pairs per sample.
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bioRxiv - Neuroscience 2023Quote: ... Libraries were sequenced Paired-End 151 bases (in addition: 8 bases for index 1 and 8 bases for index 2) setup using the NovaSeq 6000 instrument (Illumina) and the S1 Flow-Cell loaded at a final concentration in Flow-Lane loaded of 340pM and including 1% PhiX.
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bioRxiv - Bioengineering 2023Quote: Pair-end libraries (2×150 bp) were prepared from 1 µg of genomic DNA of the evolved isolates and sequenced using an MiSeq sequencer (Illumina).
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bioRxiv - Genomics 2023Quote: RNA-seq libraries were prepared from 1 μg of total RNA using the TruSeq RNA Library Prep Kit v2 (Illumina) with poly(A ...
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bioRxiv - Microbiology 2023Quote: ... All sequencing runs were performed with a spike-in of 1% PhiX control library V3 (Illumina, Cat. FC-110-3001). The sequencing mean library size was 134,629,540.5 reads [range ...
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bioRxiv - Developmental Biology 2024Quote: ... Custom paired-end sequencing (read 1 = 15 bp; read 2 = 250 bp) was performed using the NovaSeq 6000 instrument (Illumina) by the Genomics Core at Van Andel Institute ...
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bioRxiv - Microbiology 2024Quote: ... using 28 + 91 -bp paired-end reads and 8-bp Index 1 reads with a 100-cycle kit (Illumina, 20028400).
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bioRxiv - Genomics 2024Quote: ... The filtered read 1 was then mapped on the mm10 was mapped on the mm10 reference genome (source: Illumina igenomes) using the STAR package (v 2.7.9a ...
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bioRxiv - Microbiology 2020Quote: ... MDA-amplified PCR products were then diluted to DNA concentrations of 0.2 ng μL−1 and used in metagenomic library preparations with the Nextera XT DNA Library Prep Kit (Illumina, San Diego), then quantified on an Agilent 2100 Bioanalyzer System (Agilent Genomics ...
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bioRxiv - Molecular Biology 2020Quote: Libraries of eDNA and iDNA samples at 50 ng μL−1 were sequenced using a MiSeq PE300 benchtop sequencer (Illumina, USA). Library preparation was done with a TruSeq DNA PCR-Free kit (LT Lib PREP KT-ST B PhiX control v3) ...
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bioRxiv - Developmental Biology 2021Quote: ... Illumina TruSeq RNA libraries were prepared from 1 μg total RNA and sequenced to generate 50 base pair single-end reads using the the HiSeq 1500 (Illumina, USA) at the Children’s Mercy OMICs Research Core Lab (Kansas City ...
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bioRxiv - Microbiology 2019Quote: ... Illumina recommended denaturation and loading recommendations which included a 1% PhiX spike in (PhiX Control v3 Illumina Catalogue FC-110-3001). Whole genome sequencing data has been deposited in the Sequence Read Archive under PRJNA529870.
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bioRxiv - Bioengineering 2019Quote: ... sequencing adaptors and unique dual barcodes combinations were attached to each library PCR amplicons using the Nextera XT Index Kit primers (index 1 N7XX and index 2 S5XX, Illumina, CA), and the amplicon size and concentration were confirmed using a Bioanalyzer (Agilent ...
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bioRxiv - Neuroscience 2020Quote: ... RNA-seq libraries were prepared from 1 μg of total RNA using the TruSeq Stranded mRNA LT Sample Prep kit (Illumina, USA). Sequencing was performed on the Illumina NovaSeq 6000 (CNS-RNA-seq cohort subset ...
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Cis-regulatory divergence underpins the evolution of C3-C4 intermediate photosynthesis in MoricandiabioRxiv - Plant Biology 2021Quote: ... cDNA libraries were prepared using 1 μg of total RNA with the TruSeq RNA Sample Preparation Kit (Illumina, San Diego, USA). The cDNA library was qualified on the Agilent Technologies 2100 Bioanalyzer to check the library quality and fragment size of the sample ...
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bioRxiv - Cell Biology 2021Quote: ... Sequencing libraries were constructed from 1 ng of pre-amplified cDNA using DNA library preparation kit (TruePrep DNA Library Prep Kit V2 for Illumina, Vazyme). Libraries were sequenced on a HiSeq-PE150 ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 µg of total RNA was used for library preparation using the TruSeq Stranded mRNA kit (Illumina, cat# RS-122-2103) after rRNA depletion using Ribo-Zero plus (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... 1 µg of gDNA per sample was analyzed using the TruSeq® DNA PCR-Free Low-Throughput Library Prep Kit (Illumina) according to the manufacturer’s directions ...
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bioRxiv - Microbiology 2022Quote: ... and sequenced on an Illumina NovaSeq using single-end 1×100 reads with the Illumina NovaSeq SP reagent kit (Illumina, 20027464). A total of 122,190,150 raw sequence reads (mean of 4,213,453 per sample (n = 29 ...
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bioRxiv - Cell Biology 2019Quote: ... Total RNA (1 μg) was used for extracting rRNA using the Ribo-Zero rRNA Removal Kit (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... The sequencing was performed by doing a cluster generation and 150 cycles paired-end sequencing of the 24 libraries in 1 lane using the HiSeq X system and v2.5 sequencing chemistry (Illumina Inc., USA). Read data were delivered to us as fastq.
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bioRxiv - Molecular Biology 2021Quote: RNA-seq libraries were prepared with 1 µg of total RNA for each sample using the Illumina TruSeq mRNA kit (Illumina, USA) as recommended by the manufacturer using the Illumina CD RNA indexes (Illumina ...
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bioRxiv - Systems Biology 2020Quote: ... Final loading concentration of 1.8 pM and 1% PhiX (v/v) was used with the NextSeq® 500/550 High Output Kit v2 (150 cycles) (Illumina). Samples were sequenced paired-end with 75 cycles and single index of 6 cycles.
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bioRxiv - Immunology 2021Quote: ... Samples were sequenced on an Illumina Novaseq 6000 S4 flowcell (67 bp read 1 and 140 bp read 2) (Illumina, USA) to a depth of approximately 100,000 reads per cell ...
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bioRxiv - Immunology 2020Quote: ... Pooled libraries of 1 nM were denatured and diluted as per the Standard Normalization Method for the NextSeq® System (Illumina). Final loading concentration of 1.7 pM and 1% (v/v ...
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bioRxiv - Immunology 2020Quote: ... Final loading concentration of 1.7 pM and 1% (v/v) PhiX was used with the NextSeq® 500/550 High Output Kit v2 (150 cycles) (Illumina Samples were sequenced paired-end with 75 cycles of each read and single index of 6 cycles.
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bioRxiv - Immunology 2021Quote: ... whose lengths were about 600 base pairs were sequenced using an Illumina Novaseq 6000 S4 flowcell (67 bp read 1 and 140 bp read 2) (Illumina, USA). Only read2 contained the sequence regarding the definition of T cell clones.
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bioRxiv - Molecular Biology 2022Quote: ... PolyA + fraction (1 ng for each sample) was used for whole-transcriptome library preparation using ScriptSeq v2 RNA-Seq kit (Illumina, USA). Libraries amplified in 14 PCR cycles were purified using Agencourt AMPure XP beads (Beckman-Coulter ...
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bioRxiv - Neuroscience 2022Quote: ... Single-end sequencing (i.e, 1×75bp) was performed in a single run on an Illumina NextSeq 500 (Illumina, San Diego, CA). Three independent experimental replicates were run for the Cebpg shRNA-mediated knockdown and shRNA control transgenic lines for a total of 6 libraries.
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Dysregulated expanded endocannabinoid system as therapeutic targets of amyotrophic lateral sclerosisbioRxiv - Neuroscience 2024Quote: ... A library was independently prepared with 1 µg of total RNA for each sample by an Illumina TruSeq Stranded mRNA Sample Prep Kit (Illumina, Inc.). The first step in the workflow involved purifying the poly-A-containing mRNA molecules using poly-T-attached magnetic beads ...
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bioRxiv - Plant Biology 2024Quote: ... total RNA from Col-0 and hakai-1 (Mock and Hpa) three biological replicates (same samples used for Illumina RNA-seq) were pooled into a single sample ...
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bioRxiv - Microbiology 2024Quote: Libraries for Illumina short read sequencing were prepared from 1 ng of extracted DNA utilizing the Nextera XT DNA Library Prep Kit according to the manufacturer’s recommendations (Illumina Inc., USA). Sequencing was carried out in paired-end (2×300 base pairs ...
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bioRxiv - Cancer Biology 2023Quote: ... RNAseq libraries were prepared from total RNA with the QuantSeq 3′ mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen, 015.24) in combination with the UMI Second Strand Synthesis Module for QuantSeq FWD (Illumina, Read 1) (Lexogen ...
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bioRxiv - Genetics 2023Quote: ... was used to isolate total RNA and approximately 1 ug of RNA was processed with the TruSeq Stranded mRNA Library Prep Kit (Illumina 20020594) to prepare libraries for paired-end sequencing on Nova-seq 6000 ...
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bioRxiv - Developmental Biology 2023Quote: ... PolyA+ RNA-seq libraries were produced from 1 µg of RNA using the TruSeq® Stranded mRNA Library Prep (Illumina, 20020594) kit ...
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bioRxiv - Synthetic Biology 2024Quote: ... Barcoded RNA-seq libraries were prepared from 1 μg of total RNA using the TruSeq Stranded mRNA Library Prep kit according to the manufacturer’s instructions (Illumina Inc., USA). RNA-seq libraries were sequenced on an Illumina NovaSeq 6000 System according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... Amplicon sequencing libraries were prepared as previously described54,70,71 and sequenced with a 1 x 75 single end read on a NextSeq instrument (Illumina, San Diego CA). Individual gRNA reads were extracted from FASTQ files with PoolQ and analyzed for enrichment using MAGeCK72 ...
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bioRxiv - Genomics 2024Quote: Both read 1 and read 2 files from each spatial region were mapped to the mm10 reference genome (source: Illumina igenomes) using the bowtie2 package (v2.5.1 ...
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bioRxiv - Developmental Biology 2024Quote: ... at 1/8th reaction volumes (except for Cas13d control and bckdk mRNA KD samples where 1/4th of the reaction volume were used) paired with IDT for Illumina DNA/RNA UD Indexes Set A (Illumina, 20027213), and purified using the Ampure XP bead-based reagent (Beckman Coulter ...
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bioRxiv - Genomics 2021Quote: ... Total RNA (1 μg) from each sample was used for NEBNext PolyA selection and Ultra Directional RNA Library preparation (Illumina, E7490, E7420). Prepared libraries were sequenced using 2×150 bp paired-end read chemistry on an Illumina HiSeq 4000 platform.
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bioRxiv - Molecular Biology 2021Quote: ... 1 ug of treated RNA was then input into the Illumina TruSeq Small RNA Library Preparation Kit (Illumina; Cat #: RS-200-0012) with 11 cycles of PCR amplification ...
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bioRxiv - Genomics 2020Quote: ... Samples were prepared for sequencing using 1 ug of genomic DNA following the TruSeq PCR free kit protocol (Illumina, FC-121-3001). Resulting libraries were quality checked on an Agilent DNA 1000 bioanalyzer (Agilent Technologies ...