Labshake search
Citations for Illumina :
9301 - 9350 of 9484 citations for Cow Fibrinogen Like Protein 1 FGL1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... The sRNA-seq libraries were clustered and sequenced (1 × 75 bp) on a NextSeq 550 Sequencing System (Illumina) using a NextSeq 500/550 High Output Kit v2.5 (75 Cycles ...
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bioRxiv - Developmental Biology 2020Quote: ... This service utilizes the Ovation RNA-Seq System V2 kit (Nugen) for initial RNA amplification prior to library preparation and sequencing (Illumina HiSeq v4, 50bp paired-end reads). For each genotype ...
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bioRxiv - Evolutionary Biology 2020Quote: ... modified with 5’-overhangs designed to match the Illumina sequencing adapters and molecular identifiers (MIDs) of the Nextera® XT v2 Index Kit (Illumina Inc., San Diego, CA, USA). The second library included eight technical duplicates ...
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bioRxiv - Evolutionary Biology 2021Quote: ... All of the subsequent steps were performed by the CRG Genomics Unit (Barcelona): sequencing libraries were prepared from the pure high molecular weight DNA using TruSeq DNA HT Library Preparation kit (Illumina ® HiSeq ® Sequencing v4 Chemistry). A paired-end library with a target insert size of ∼500 bp was sequenced on an Illumina® HiSeq2500 platform in paired-end mode ...
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bioRxiv - Developmental Biology 2021Quote: ... We assessed the quality of RNA using fragment analysis to obtain RNA integrity number (RIN) before proceeding with Library preparation (NEBNext® Ultra™ RNA Library Prep Kit, Illumina, San Diego, CA, USA). Each sample was sequenced in Illumina HiSeq 2000 (Single read ...
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bioRxiv - Microbiology 2022Quote: The 16S and ITS libraries were prepared by the sequencing facility using the Illumina TruSeq≡Nano DNA Library Prep Kit (Illumina, Inc., San Diego, CA, United States), llumina MiSeq was run using a v2 Standard sequencing format with paired end reads (2 x 250 bp) ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA quality control was performed in a fragment analyzer and sequencing libraries were constructed using a Nextera DNA Flex kit (now renamed to Illumina DNA Prep, Illumina, San Diego, CA) and 2×150 BP paired-end sequencing was performed in an Illumina NextSeq500 ...
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bioRxiv - Genomics 2020Quote: A total of 720 metagenomic libraries were prepared using Illumina-specific Nextera DNA transposase adapters and a Tagment DNA Enzyme and Buffer Kit (Illumina, San Diego, CA, cat. no. 20034197) (modified from Baym et al ...
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bioRxiv - Systems Biology 2020Quote: ... Supernatants were discarded and nuclei were re-suspended in 50 μl reaction buffer (2.5 μl of Tn5 transposase and 25 μl of TD buffer from a Nextera DNA Library Prep Kit from Illumina, and 22.5 μl nuclease-free H2O). The reaction was incubated at 37°C for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... A second PCR was performed to attach dual indices and Illumina® sequencing adapters using the Nextera XT Index kit (Illumina®; San Diego, CA, USA). The conditions of this PCR included ...
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bioRxiv - Microbiology 2020Quote: ... WGS libraries were sequenced with paired-end 150 base pair reads on an Illumina NovaSeq 6000 by the UW Biotechnology Center (Illumina NovaSeq 6000 S1 Reagent Kit v1.5).
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bioRxiv - Cell Biology 2019Quote: ... transcripts were purified from total RNA and mRNA sequencing libraries were prepared with the TruSeq Stranded mRNA Library Preparation Kit (Illumina, RS-122-2101, RS- 122-2102). The detailed protocol for RNA sequencing was discussed in earlier (34 ...
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bioRxiv - Microbiology 2019Quote: ... A paired-end 250-bp sequencing run was performed using the Illumina MiSeq instrument according to the MiSeq™ Reagent Kit v2 Preparation Guide (Illumina, Inc., San Diego, CA, USA).
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bioRxiv - Microbiology 2023Quote: ... and short read sequencing using the Illumina platform (Illumina DNA Prep kit and IDT 10bp UDI indices, and sequenced on an Illumina NextSeq 2000 producing 2×151bp reads) was performed by the Microbial Genome Sequencing Center ...
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bioRxiv - Plant Biology 2023Quote: ... RNA libraries were prepared from samples that met the Illumina TruSeq® Stranded Total RNA Sample Preparation Guide (15031048 E) input guidelines using the Illumina TruSeq® Stranded Total (Plant) RNA Sample Preparation kit (Illumina Inc., San Diego, California, USA). For each library preparation ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries for each sample were prepared from ≥200 ng of sheared DNA with TruSeq® Nano DNA High Throughput Library Prep Kit (20015965, Illumina Inc., San Diego, CA, USA) and IDT for Illumina – TruSeq® DNA UD Indexes v1 (Illumina Inc. ...
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bioRxiv - Genomics 2023Quote: ... we processed 500 ng of total RNA using the TruSeq Stranded mRNA Library Prep kit (Illumina, Cat # 20020595; Indexes: IDT for Illumina TruSeq RNA UD Indexes, cat # 20020591). The resulting libraries were then analyzed on a TapeStation (Agilent ...
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bioRxiv - Neuroscience 2024Quote: ... Sequencing libraries were generated with Illumina Stranded mRNA Prep kit and sequencing of 2×50 bp paired-end reads was performed on an Illumina NovaSeq 6000 (Illumina Inc., San Diego, CA, United States). The sequences were aligned to the human GRCh38 reference genome using STAR (58) ...
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bioRxiv - Genomics 2022Quote: ... Libraries for Illumina sequencing were generated with NEBNext® Ultra™ RNA Library Prep Kit for Illumina and sequenced on the Illumina NovaSeq6000 (Illumina, Inc., San Diego, CA, USA). Libraries for long-read sequencing were generated with the cDNA PCR sequencing kit (SQK-PCS109 ...
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bioRxiv - Immunology 2019Quote: ... The fragmented mRNA samples were subjected to cDNA synthesis using Illumina TruSeq® RNA sample preparation kit version 2 (Illumina, #RS-122-2001 and #RS-122-2002) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... and sequenced on an Illumina NextSeq 500 system using the NextSeq 500/550 Mid Output Kit v2.5 (#20024904, Illumina; 150 cycles, paired-end run 2x 75 bp) with an average of 1×107 reads per RNA sample.
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bioRxiv - Cancer Biology 2023Quote: ... Triplicates were pooled for a target total cell number of 60,000 in 50uL to load less than 20uL into the 10X controller or perform TDE1-based tagmentation reactions on small volumes (Illumina Tagment DNA TDE1 Enzyme and Buffer Kit). The 10X Genomics Chromium Platform was used to generate a targeted 5,000 single cell Gel Bead-In-Emulsions (GEMs ...
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bioRxiv - Cancer Biology 2023Quote: ... The library preparation for bulk tumors and cell lines was generated using the TruSeq Stranded mRNA sample preparation kit (Illumina Inc., San Diego, RS-122-2101/2) according to the manufacturer’s instructions (Illumina ...
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bioRxiv - Genomics 2024Quote: ... bidentatus DNA extraction and library preparation was performed by Dovetail Genomics (Scotts Valley, CA, USA) with the TruSeq DNA PCR-Free kit (Trueq Index 5, Illumina, San Diego, CA, USA, mean size 475bp). 150 bp paired-end sequencing was performed on one lane of HiSeqX by Novogene (Cambridge ...
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bioRxiv - Developmental Biology 2024Quote: ... we prepared n=40 sequencing libraries from 1µg total RNA per sample using Illumina® Stranded mRNA Prep kits with unique 5′ and 3′ index adapter pairs (IDT for Illumina® RNA UD Indexes, Set A). Libraries were PCR amplified for 10 cycles ...
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bioRxiv - Cell Biology 2020Quote: ... Whole genome sequencing was performed with 1 μg of the digested DNA using a HiSeq X Ten Sequencer (Illumina) at Macrogen (South Korea).
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bioRxiv - Evolutionary Biology 2021Quote: ... eurysternus samples (complete metadata including barcodes are available in Supplementary data 1. The purified library was sequenced on Illumina Miseq using V2 chemistry with 500 cycles (Norwegian High Throughput Sequencing Centre ...
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bioRxiv - Cancer Biology 2019Quote: ... Sequencing was performed on an Illumina NovaSeq platform (paired-end, Read 1: 26 cycles and Read 2: 98 cycles) according to the manufacturer’s instructions (Illumina).
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bioRxiv - Physiology 2020Quote: Sequencing of the library was performed at a final concentration of 1.8 pM and with a 1 % PhiX v3 control library spike-in (Illumina) on the NextSeq500 sequencing system (Illumina) ...
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bioRxiv - Genetics 2019Quote: ... We tagmented 5 ng of cDNA using 1 uL Nextera Tagment DNA Tn5 transposase (Illumina, San Diego, CA, 15027916) in a 10 uL tagmentation mix for 10 minutes at 55 °C.
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bioRxiv - Microbiology 2019Quote: ... 1 µg purified total RNA was used as input for the Truseq stranded total RNA Gold library prep (Illumina). Ribosomal RNA was depleted using Ribo-Zero H/M/R Gold and depleted RNA fragmented for 8min at 94C ...
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bioRxiv - Genomics 2019Quote: ... and the libraries were prepared from 1 µg of RNA using TruSeq RNA Library Prep (Illumina, San Diego, CA). These samples were sequenced on a Illumina HiSeq 2000 lane as 75 bp single ends ...
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bioRxiv - Cancer Biology 2021Quote: ... adding Illumina sequencing adapters and dual-index barcodes (index 1(i7) and index 2(i5)) (Illumina, San Diego, CA) to the amplicon target ...
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bioRxiv - Bioengineering 2022Quote: ... and 1 ×107 cells from each gate were barcoded and the sequences for each cell were determined by Illumina next-generation sequencing15 ...
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bioRxiv - Genomics 2022Quote: Combined with four types of libraries constructed: (1) the short-read sequencing library with 350-bp insertions from Illumina HiSeq X Ten instrument platforms ...
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bioRxiv - Immunology 2020Quote: ... Libraries were sequenced on the iSeq (cases 1 - 6 and controls) or NovaSeq 6000 (case 7 and controls) (Illumina) using 150nt paired-end reads.
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bioRxiv - Microbiology 2021Quote: ... All cDNA libraries were sequenced on an Illumina NextSeq 500 instrument at the Boston University microarray and sequencing core according to Illumina and 10x Genomics guidelines with 1.4-1.8pM input and 1% PhiX control library spike-in (Illumina, USA).
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bioRxiv - Molecular Biology 2022Quote: ... The chromatin was then tagmented by resuspending beads in 29 µl Tagmentation Buffer (10 mM Tris-HCl pH 8.0, 5 mM MgCl2, 10% dimethylformamide) and adding 1 µl of transposase (Illumina). Samples were incubated at 37 °C for 10 min and the reaction was terminated by adding 150 µl RIPA buffer ...
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bioRxiv - Genetics 2022Quote: ... sequence reads were mapped to the mouse reference genome GRCm38/mm10 (iGenomes, Illumina; chromosomes 1-19, X, Y, M) using the Rsubread v1.28.1 package in R v3.4.4 ...
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bioRxiv - Systems Biology 2019Quote: ... Washed AMPure XP beads attached to double-stranded cDNAs were directly eluted using 3.75 μL of 1× diluted Tagment DNA Buffer (Illumina) and mixed well using a vortex mixer and pipetting ...
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bioRxiv - Genetics 2020Quote: ... for PPI (see supplementary methods).14 The 148 additionally selected SNV markers (supplementary table 1) were genotyped in 1,012 F2 mice by Illumina BeadArray genotyping (Illumina Golden Gate assay ...
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bioRxiv - Genomics 2021Quote: ... 30 μL of NEBNext Ultra II Ligation Master Mix, 1 μL of NEBNext Ligation Enhancer, 2.5 μL of NEBNext Adapter for Illumina) at 20 °C for 15 min. ...
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bioRxiv - Developmental Biology 2022Quote: ... The PCR product was purified in a 1 % agarose gel and sequenced using a NextSeq 550 and NovaSeq (Illumina) to obtain 79+79-nt ...
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bioRxiv - Plant Biology 2022Quote: The triplicate total RNA samples used for single-end 1×75bp RNA-Seq on a NextSeq500 sequencing platform (Illumina). After examining the data quality using FastQC (v0.11.5) ...
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bioRxiv - Genomics 2022Quote: ... 5 μl of Index primer (both provided in NEBNext Multiplex Oligos for Illumina Index Primers Sets 1 and 2), and 25 μl NEBNext Ultra II Q5 Master Mix (PCR cycling conditions ...
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bioRxiv - Plant Biology 2023Quote: ... The library was diluted to 0.5 nM with EB in 18 μL and spiked with 1% PhiX Control v3 (Illumina) before being prepared for loading using a NovaSeq XP 2 lane kit v1.5 (20043130 ...
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Resolution of SARS-CoV-2 infection in human lung tissues is driven by extravascular CD163+ monocytesbioRxiv - Immunology 2024Quote: ... All cDNA libraries were sequenced on an Illumina NextSeq 500 instrument at the Boston University microarray and sequencing core according to Illumina and 10x Genomics guidelines with 1.4-1.8pM input and 1% PhiX control library spike-in (Illumina, USA).
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bioRxiv - Pathology 2023Quote: ... All cDNA libraries were sequenced on an Illumina NextSeq 500 instrument according to Illumina and 10X Genomics guidelines with 1.4–1.8 pM input and 1% PhiX control library spike-in (Illumina, USA). Sequencing data were processed using 10X Genomics’ Cell Ranger pipeline to generate feature/barcode matrices from raw count data.
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bioRxiv - Developmental Biology 2023Quote: ... bead-bound complexes were resuspended in tagmentation mixture (25µL reaction containing 1 µL of enzyme in 1X buffer, Illumina) and incubated at 37°C for 25minutes ...
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bioRxiv - Pathology 2023Quote: ... Barcoded libraries from each experimental sample were combined in equimolar concentrations of 1.5 pM prior to sequencing at 75bp x 1 (single-end) read metric on a NextSeq 550 (Illumina) system.