Labshake search
Citations for Illumina :
9151 - 9193 of 9193 citations for Mouse CXCL2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and sequenced with a 2×250bp paired-end configuration on an Illumina MiSeq platform applying the MiSeq v2 500-cycle sequencing kit (Illumina Inc., San Diego, CA, USA).
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bioRxiv - Immunology 2023Quote: ... RNA-seq libraries were generated via mRNA isolation from total RNA by polyA-positive selection using the TruSeq™ RNA/DNA Library Preparation Kit (Illumina #RS-122-2001, USA) prior to sequencing using the NextSeq™ 550 system (Illumina).
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bioRxiv - Cancer Biology 2023Quote: ... we dispensed 600 nL of tagmentation reaction mix containing Tagmentation DNA buffer (TD) and Amplicon Tagment Mix (ATM) at 2:1 v/v ratio (Nextera kit, Illumina, cat. no. FC-131-1096) into each well and performed tagmentation at 55° C for 5 min followed by hold at 4° C in a PCR thermocycler ...
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bioRxiv - Genomics 2023Quote: ... libraries were prepared from liver tissues using an Illumina TruSeq Nano DNA Library Prep Kit and paired-end (PE150) sequenced on the Illumina Novaseq 6000 sequencing platform (Illumina, Inc., San Diego, CA, USA). We used gonad tissues for ONT PromethION library preparation and sequencing ...
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bioRxiv - Microbiology 2023Quote: ... Library preparation for VLP genomic DNA involved using >25ng input DNA with the Nextera DNA Flex Library Preparation Kit (currently named Illumina DNA prep, Illumina, CA, USA). Deviating from the Illumina protocol’s full reaction volume (50 µL) ...
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bioRxiv - Cell Biology 2023Quote: cDNA libraries were prepared from 1 μg total RNA of each sample using the TruSeq Stranded mRNA Sample Prep Kit (Illumina, Inc., San Diego, CA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... The subsequent steps of library preparation were all carried out on the Agilent NGS Bravo workstation in 96-well plates following the instructions for the Illumina TruSeq Stranded mRNA kit (Illumina Cat no: 20020595, Illumina, USA). mRNA was purified from 300-800 ng of total RNA through selective-binding on poly dT-coated beads ...
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bioRxiv - Microbiology 2024Quote: ... Parallel aliquots of the DNA samples used for ONT sequencing were used to prepare Illumina libraries using the Nextera DNA Flex Library Prep Kit as per protocol (Illumina Inc., San Diego, CA, USA). Library quality was measured using an Agilent 4200 TapeStation System as described above ...
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bioRxiv - Genomics 2024Quote: ... The libraries were prepared with Ampliseq Library Plus for Illumina kit (Illumina, San Diego, CA, USA and using Illumina’s Ampliseq CD indexes set A(Illumina), and their quality was assessed with High Sensitivity DNA reagents on an Agilent 2100 Bioanalyzer System ...
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bioRxiv - Genetics 2024Quote: ... Samples were preamplified using High-Fidelity 2X PCR Master Mix (New England Bio-labs, #M0541L) and adapters (Illumina Nextera XT Index kit #FC-131-1001). The number of additional cycles was determined by quantitative PCR ...
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bioRxiv - Microbiology 2024Quote: ... The pool was run at a final concentration of 10pM on an Illumina MiSeq instrument using MiSeq® Reagent Kit v3 (600 cycle) (Illumina Catalogue FC-102-3003) following the Illumina recommended denaturation and loading recommendations which included a 20% PhiX spike in (PhiX Control v3 Illumina Catalogue FC-110-3001) ...
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bioRxiv - Developmental Biology 2020Quote: ... This service utilizes the Ovation RNA-Seq System V2 kit (Nugen) for initial RNA amplification prior to library preparation and sequencing (Illumina HiSeq v4, 50bp paired-end reads). For each genotype ...
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bioRxiv - Evolutionary Biology 2020Quote: ... modified with 5’-overhangs designed to match the Illumina sequencing adapters and molecular identifiers (MIDs) of the Nextera® XT v2 Index Kit (Illumina Inc., San Diego, CA, USA). The second library included eight technical duplicates ...
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bioRxiv - Evolutionary Biology 2021Quote: ... All of the subsequent steps were performed by the CRG Genomics Unit (Barcelona): sequencing libraries were prepared from the pure high molecular weight DNA using TruSeq DNA HT Library Preparation kit (Illumina ® HiSeq ® Sequencing v4 Chemistry). A paired-end library with a target insert size of ∼500 bp was sequenced on an Illumina® HiSeq2500 platform in paired-end mode ...
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bioRxiv - Developmental Biology 2021Quote: ... We assessed the quality of RNA using fragment analysis to obtain RNA integrity number (RIN) before proceeding with Library preparation (NEBNext® Ultra™ RNA Library Prep Kit, Illumina, San Diego, CA, USA). Each sample was sequenced in Illumina HiSeq 2000 (Single read ...
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bioRxiv - Microbiology 2022Quote: The 16S and ITS libraries were prepared by the sequencing facility using the Illumina TruSeq≡Nano DNA Library Prep Kit (Illumina, Inc., San Diego, CA, United States), llumina MiSeq was run using a v2 Standard sequencing format with paired end reads (2 x 250 bp) ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA quality control was performed in a fragment analyzer and sequencing libraries were constructed using a Nextera DNA Flex kit (now renamed to Illumina DNA Prep, Illumina, San Diego, CA) and 2×150 BP paired-end sequencing was performed in an Illumina NextSeq500 ...
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bioRxiv - Genomics 2020Quote: A total of 720 metagenomic libraries were prepared using Illumina-specific Nextera DNA transposase adapters and a Tagment DNA Enzyme and Buffer Kit (Illumina, San Diego, CA, cat. no. 20034197) (modified from Baym et al ...
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bioRxiv - Systems Biology 2020Quote: ... Supernatants were discarded and nuclei were re-suspended in 50 μl reaction buffer (2.5 μl of Tn5 transposase and 25 μl of TD buffer from a Nextera DNA Library Prep Kit from Illumina, and 22.5 μl nuclease-free H2O). The reaction was incubated at 37°C for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... A second PCR was performed to attach dual indices and Illumina® sequencing adapters using the Nextera XT Index kit (Illumina®; San Diego, CA, USA). The conditions of this PCR included ...
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bioRxiv - Cancer Biology 2020Quote: ... and 1 μl of each Nextera XT Index Kit v2 set B or set C barcoding primer (Illumina, Cat# FC-131-2002 or FC-131-2003). These reactions were then incubated at 95°C for 15 minutes ...
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bioRxiv - Microbiology 2020Quote: ... WGS libraries were sequenced with paired-end 150 base pair reads on an Illumina NovaSeq 6000 by the UW Biotechnology Center (Illumina NovaSeq 6000 S1 Reagent Kit v1.5).
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bioRxiv - Cell Biology 2019Quote: ... transcripts were purified from total RNA and mRNA sequencing libraries were prepared with the TruSeq Stranded mRNA Library Preparation Kit (Illumina, RS-122-2101, RS- 122-2102). The detailed protocol for RNA sequencing was discussed in earlier (34 ...
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bioRxiv - Microbiology 2019Quote: ... A paired-end 250-bp sequencing run was performed using the Illumina MiSeq instrument according to the MiSeq™ Reagent Kit v2 Preparation Guide (Illumina, Inc., San Diego, CA, USA).
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bioRxiv - Microbiology 2023Quote: ... and short read sequencing using the Illumina platform (Illumina DNA Prep kit and IDT 10bp UDI indices, and sequenced on an Illumina NextSeq 2000 producing 2×151bp reads) was performed by the Microbial Genome Sequencing Center ...
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bioRxiv - Plant Biology 2023Quote: ... RNA libraries were prepared from samples that met the Illumina TruSeq® Stranded Total RNA Sample Preparation Guide (15031048 E) input guidelines using the Illumina TruSeq® Stranded Total (Plant) RNA Sample Preparation kit (Illumina Inc., San Diego, California, USA). For each library preparation ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries for each sample were prepared from ≥200 ng of sheared DNA with TruSeq® Nano DNA High Throughput Library Prep Kit (20015965, Illumina Inc., San Diego, CA, USA) and IDT for Illumina – TruSeq® DNA UD Indexes v1 (Illumina Inc. ...
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bioRxiv - Genomics 2023Quote: ... we processed 500 ng of total RNA using the TruSeq Stranded mRNA Library Prep kit (Illumina, Cat # 20020595; Indexes: IDT for Illumina TruSeq RNA UD Indexes, cat # 20020591). The resulting libraries were then analyzed on a TapeStation (Agilent ...
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bioRxiv - Neuroscience 2024Quote: ... Sequencing libraries were generated with Illumina Stranded mRNA Prep kit and sequencing of 2×50 bp paired-end reads was performed on an Illumina NovaSeq 6000 (Illumina Inc., San Diego, CA, United States). The sequences were aligned to the human GRCh38 reference genome using STAR (58) ...
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bioRxiv - Genomics 2022Quote: ... Libraries for Illumina sequencing were generated with NEBNext® Ultra™ RNA Library Prep Kit for Illumina and sequenced on the Illumina NovaSeq6000 (Illumina, Inc., San Diego, CA, USA). Libraries for long-read sequencing were generated with the cDNA PCR sequencing kit (SQK-PCS109 ...
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bioRxiv - Immunology 2019Quote: ... The fragmented mRNA samples were subjected to cDNA synthesis using Illumina TruSeq® RNA sample preparation kit version 2 (Illumina, #RS-122-2001 and #RS-122-2002) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... and sequenced on an Illumina NextSeq 500 system using the NextSeq 500/550 Mid Output Kit v2.5 (#20024904, Illumina; 150 cycles, paired-end run 2x 75 bp) with an average of 1×107 reads per RNA sample.
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bioRxiv - Cancer Biology 2023Quote: ... Triplicates were pooled for a target total cell number of 60,000 in 50uL to load less than 20uL into the 10X controller or perform TDE1-based tagmentation reactions on small volumes (Illumina Tagment DNA TDE1 Enzyme and Buffer Kit). The 10X Genomics Chromium Platform was used to generate a targeted 5,000 single cell Gel Bead-In-Emulsions (GEMs ...
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bioRxiv - Cancer Biology 2023Quote: ... The library preparation for bulk tumors and cell lines was generated using the TruSeq Stranded mRNA sample preparation kit (Illumina Inc., San Diego, RS-122-2101/2) according to the manufacturer’s instructions (Illumina ...
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bioRxiv - Genomics 2024Quote: ... bidentatus DNA extraction and library preparation was performed by Dovetail Genomics (Scotts Valley, CA, USA) with the TruSeq DNA PCR-Free kit (Trueq Index 5, Illumina, San Diego, CA, USA, mean size 475bp). 150 bp paired-end sequencing was performed on one lane of HiSeqX by Novogene (Cambridge ...
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bioRxiv - Developmental Biology 2024Quote: ... we prepared n=40 sequencing libraries from 1µg total RNA per sample using Illumina® Stranded mRNA Prep kits with unique 5′ and 3′ index adapter pairs (IDT for Illumina® RNA UD Indexes, Set A). Libraries were PCR amplified for 10 cycles ...
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bioRxiv - Microbiology 2020Quote: ... Samples that met the Illumina sample input guidelines were prepared according the TruSeq® Stranded mRNA Sample Preparation Guide (Rev. E) using the Illumina® TruSeq® Stranded mRNA Sample Preparation kit (Illumina Inc., San Diego, California, USA). For each library preparation ...
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bioRxiv - Cancer Biology 2020Quote: ... The PCR-amplified cDNA was purified using AMPure XP beads and processed with the Nextera XT DNA Library Preparation Kits (Illumina, Cat. # FC-131-1024 and FC-131-1096) as per the protocol provided by the manufacturer.
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bioRxiv - Genetics 2020Quote: ... Libraries were made according to the manufacturer’s directions for the TruSeq Stranded mRNA LT Sample Prep Kit – sets A and B (Illumina, Cat. No. RS-122-2101 and RS-122-2102). Resulting short fragment libraries were checked for quality and quantity using the Bioanalyzer (Agilent ...
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bioRxiv - Molecular Biology 2023Quote: ... and sequenced on an Illumina NovaSeq 6000 system using the NovaSeq 6000 S4 Individual Lane Loading Reagent kit (#20028313, Illumina; 150 cycles, paired end run 1x 111 bp) with an average of 2×107 reads per sample ...
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bioRxiv - Cancer Biology 2022Quote: ... ribosomal RNA depleted strand-specific RNA libraries with the TruSeq Stranded Total RNA LT sample preparation kit with Ribo-Zero Gold (Illumina, cat# RS-122-2301 and #RS-122-2302), and transcriptome capture based libraries with the TruSeq RNA Access Library Prep Kit (Illumina ...
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bioRxiv - Cancer Biology 2023Quote: ... ribosomal RNA depleted strand-specific RNA libraries with the TruSeq Stranded Total RNA LT sample preparation kit with Ribo-Zero Gold (Illumina, cat# RS-122-2301 and #RS-122-2302), and transcriptome capture based libraries with the TruSeq RNA Access Library Prep Kit (Illumina ...
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bioRxiv - Genomics 2020Quote: ... was performed on an Illumina NextSeq machine using either a 300 cycle v2 mid output or high output kit (Illumina, Code FC-404-2003 or Code FC-404-2004) under standard Illumina® procedure as previously described[9].